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PubMedDate not supplied

Sensitivity of Trichomonas vaginalis, Tritrichomonas foetus and Giardia intestinalis to bacitracin and its zinc salt in vitro.

The activity of bacitracin in vitro against Giardia intestinalis, Trichomonas vaginalis and Tritrichomonas foetus was enhanced 5-10 times by equimolar concentrations of zinc. Bacitracin and bacitracin zinc activity was tolerant to either aerobic or anaerobic culture conditions and equally effective against T. vaginalis isolates sensitive or insensitive to metronidazole. This enhancement was not due to zinc toxicity and was zinc dose dependent.

Animals↗
PubMed1985

Bacitracin enhances intracellular accumulation of insulin in rat adipocytes.

Bacitracin (1 mg/ml) markedly increased (approx. 75%) the cell-associated specifically bound 125I-labelled insulin without altering the affinity of the binding sites. Bacitracin also exerted a modest inhibitory effect on the degradation of insulin in the incubation medium determined as radioactivity not precipitated by trichloroacetic acid (from 9.6 to 4.8%). The effect on insulin binding was about 5-times as sensitive as the effect on degradation. The increased binding was due to intracellular accumulation of radioactivity which could not be removed by treating the cells with trypsin. This increase was not seen when the internalization process was reduced by ATP-depletion or low temperature. Since the trypsin-sensitive fraction of cell-associated radioactivity was apparently not altered, it is suggested that bacitracin, in addition to its well-known inhibition of extracellular degradation, also inhibits the intracellular degradation of insulin.

3-O-Methylglucose↗
PubMed1980

Hormonal measurement in rat anterior pituitary cell cultures: loss of immunoreactive LH counteracted by fetal calf serum and bacitracin.

Immunoassayable LH in media samples from rat anterior pituitary cell cultures declines during storage and only 20% of the LH remains after 4 weeks at -20 degrees C. The LH loss was not due to bacterial contamination or to damage to the hormone from repeated freezing and thawing. SDS-PAGE of 125IrLH in media samples showed greater recovery of 125IrLH when 1 mM bacitracin or 2% fetal calf serum were present in the medium. The ratio of intact: subunit 125IrLH was unchanged by the presence of bacitracin or fetal calf serum indicating that the loss of immunoreactive LH was not due to dissociation of intact hormone. LH appears to be irreversibly altered in stored culture media, a process which can be prevented by the addition of bacitracin or fetal calf serum to the media prior to storage. The use of either substance allows accurate and reproducible measurement of LH released from pituitary cells in culture.

Animals↗
PubMed2004

Mechanism of bacitracin permeation enhancement through the skin and cellular membranes from an ethosomal carrier.

The main objective of the present work was to investigate the dermal and intracellular delivery of bacitracin, a model polypeptide antibiotic, from ethosomes. Bacitracin and fluorescently labeled bacitracin (FITC-Bac) ethosomes were characterized for shape, lamellarity, fluidity, size distribution and entrapment capacity by scanning electron microscopy (SEM), transmission electron microscopy (TEM), differential scanning calorimetry (DSC), dynamic light scattering (DLS) and ultracentrifugation, respectively. Confocal laser scanning microscopy (CLSM) experiments revealed that ethosomes facilitated the copenetration of antibiotic and phospholipid into cultured 3T3 Swiss albino mice fibroblasts. These results, confirmed by data obtained in fluorescent-activated cell sorting (FACS) experiments, suggest that ethosomes penetrate cellular membrane releasing the entrapped molecule within cells. Additional work was focused on skin permeation behavior of FITC-Bac from ethosomal systems in in vitro and in vivo experiments through human cadaver and rat skin, respectively. These studies demonstrated that the antibiotic peptide was delivered into deep skin layers through intercorneocyte lipid domain of stratum corneum (SC). Occlusion had no effect on the permeation profile of the drug from ethosomes in in vitro experiments. Efficient delivery of antibiotics to deep skin strata from ethosomal applications could be highly beneficial, reducing possible side effects and other drawbacks associated with systemic treatment. Furthermore, ethosomal delivery systems could be considered for the treatment of a number of dermal infections, requiring intracellular delivery of antibiotics, whereby the drug must bypass two barriers: the SC and the cell membrane.

3T3 Cells↗
PubMed1999

EXAFS study of zinc coordination in bacitracin A.

Bacitracin is a dodecapeptide antibiotic produced by Bacillus sp. The antibacterial activity depends upon the peptide binding a divalent metal. Hitherto, the exact coordination of the cation has not been established. In particular the role played by the sulphur and nitrogen atoms of the thiazoline ring of bacitracin A has not been clear. Here the coordination of Zn2+ by bacitracin A has been studied using extended X-ray absorption fine structure. The experimental data are consistent with a model in which zinc is coordinated by one oxygen and three nitrogen atoms with the sulphur atom of the thiazoline ring not being directly involved in the zinc coordination.

Amines↗
PubMed1987

Allergic contact dermatitis from bacitracin.

Bacitracin is a commonly used topical antibiotic. Although occasional reports of adverse reactions to bacitracin have appeared in the medical literature, it is considered to be a nonsensitizing agent by most dermatologists. A description of two patients who demonstrated allergic contact dermatitis from bacitracin is presented, with a review of the pertinent literature.

Adult↗
PubMed1978

Structure analysis of small proteins by electron microscopy: valinomycin, bacitracin and low molecular weight cell growth stimulators.

Dark field electron microscopy was combined with optical filtering to study at high resolution the structure of the cyclopeptide antibiotics, bacitracin and valinomycin, and two proteins of unknown structure, LMW-CSA N and B, low molecular weight granulocyte colony stimulating activity isolated from medium conditioned with normal or leukemic leukocytes. For bacitracin and valinomycin the images faithfully represented the known structural features at a resolution of 0.5 nm or better, depicting a two-ring structure for bacitracin, as well as the position of the potassium ion in valinomycin. Both proteins of unknown structrue had at least one cyclic peptide portion. LMW-CSA N had a size of 2.0 nm, LMW-CSA B of 2.4 nm. A potential site of the calcium ionophoric activity in the latter protein was found to be in the larger of the two ring portions constituting the molecule.

Bacitracin↗
PubMed2001

Optimization of HPLC method for stability testing of bacitracin.

A stability indicating HPLC assay for bacitracin has been developed and validated. The assay is based on a gradient elution, reversed phase column and UV diode array detection. On the basis of our previous analytical work several additional systematic HPLC tests for optimization of analytical method were performed. In order to achieve the highest selectivity of HPLC method, tests were conducted with extremely complex samples -- zinc bacitracin feed grade as food additive for animals. The influence of pH of mobile phase and type of columns on chromatographic separation of active (A, B(1) and B(2)) and inactive (F) polypeptide components of bacitracin were investigated in detail. It was found also that the peak B(1) comprises three and the peak F two subunits -- probably isomers. The obtained analytical procedure proved to be very selective and effective for the simultaneous determination of active polypeptide A, B(1) and B(2), impurities, known and unknown degradation products and ballast material.

Anti-Bacterial Agents↗
PubMed1986

Insulin binding to cultured adult hepatocytes. Effects of bacitracin and chloroquine on the nature of cell-associated radioactivity.

Sephadex (G-50 fine grade)-gel chromatography and trichloroacetic acid (TCA) precipitation were used to investigate the effects of chloroquine and bacitracin on the nature of cell-associated radioactivity in studies on the binding and degradation of 125I-insulin in cultured rat hepatocytes. Sephadex peak I, eluted with the void volume, increased with hepatocyte incubation time and comprised 6% of total cell-bound radioactivity at 120 min. However, all radioactivity in this peak was due to unspecific binding. Peak II, corresponding to intact insulin, represented 95% of specifically cell-associated label at 5 min and decreased to 77% at 120 min. Peak III, containing the final low-Mr degradation products, increased with incubation time (22% of specifically bound label at 120 min). The TCA-precipitable and TCA-soluble fractions of hepatocytes extracted with 0.1% SDS were within 4-7% of the proportions of radioactivity in peaks II and III respectively. Scatchard plots based on insulin-binding data from Sephadex chromatography or TCA precipitation were identical. Dissociation studies revealed that at least 75% of the intact insulin associated with the hepatocytes was bound to receptors at the cell surface. Bacitracin increased the proportion of cell-associated intact hormone and decreased that of ligand degraded when analysed by either Sephadex chromatography or TCA precipitation. The proportion of surface-bound to internalized intact hormone remained unaltered, indicating that bacitracin acted predominantly at the cell surface. In the presence of chloroquine, which dramatically increased the contribution of peak I to specific binding, 'intact' insulin was substantially overestimated when determined as the TCA-precipitable fraction. In addition, all peak I material and 50% of cell-associated label in peak II was trapped intracellularly, thereby pointing to the lysosomal or prelysosomal site of action of this drug.

Animals↗
PubMed1972

Inhibition of sterol biosynthesis by bacitracin.

Bacitracin is an inhibitor of the biosynthesis of squalene and sterols from mevalonic acid, C(5)-isopentenyl pyrophosphate, or C(15)-farnesyl pyrophosphate catalyzed by preparations from rat liver. The antibiotic is active at extremely low ratios of antibiotic to substrate. The mechanism of inhibition appears to be the formation of a complex between bacitracin, divalent cation, and C(15)-farnesyl pyrophosphate and other isoprenyl pyrophosphates. It is similar to the formation of the complex with C(55)-isopropenyl pyrophosphate in microbial systems. The toxicity of bacitracin for animal cells could be due in part to the formation of these complexes.

Alkaline Phosphatase↗
PubMed2001

Influence of zinc bacitracin, light regimen and dustbathing on the health and welfare of broiler chickens.

1. The influence of zinc bacitracin, an antibiotic growth promoter, and the combined influence of two environmental factors (8 h of darkness and access to sand) on health and welfare variables were studied in 96 broiler chickens (kept as pairs). 2. Chickens fed with zinc bacitracin (ZB) had a higher frequency of tibial dyschondroplasia (TD) and a higher frequency of dustbathing than chickens fed without zinc bacitracin. 3. When 42 d old, chickens exposed to 8 h of darkness and access to sand were heavier than chickens reared under continuous light and with no access to sand. 4. Fluctuating asymmetry was higher in the 'light/no sand' groups than in the 'night/sand + ZB' group. 5. There was a negative correlation between tonic immobility and weight and a positive correlation between weight and TD.

Animal Husbandry↗
PubMed1987

Responses of broilers to dietary zinc bacitracin at two different planes of nutrition.

Four concentrations of Zn bacitracin (0, 50, 100 and 150 mg/kg) added to diets of two different planes of nutrition were fed to male broiler chicks for 7 weeks. Feeding broilers Zn bacitracin significantly improved growth rate and food conversion, and increased shank pigmentation. The effects of Zn bacitracin were independent of the plane of nutrition. Regression analyses revealed that the responses of birds were linear.

Animal Nutritional Physiological Phenomena↗
PubMed1996

The influence of Lactobacillus acidophilus and bacitracin on layer performance of chickens and cholesterol content of plasma and egg yolk.

1. The influence of Lactobacillus acidophilus alone or in combination with zinc bacitracin on the performance of laying hens was monitored over a period of 4 months. 2. Lactobacillus acidophilus improved egg production, food conversion and reduced the cholesterol concentration in the eggs, but zinc bacitracin had no effect when administered alone. 3. In combination, bacitracin had an adverse effect on the otherwise beneficial activity of the culture.

Animal Feed↗
PubMed1999

Bacitracin versus mupirocin for Staphylococcus aureus nasal colonization.

We performed a randomized prospective study of 5-day treatment with topical mupirocin or bacitracin for the elimination of Staphylococcus aureus nasal colonization in healthcare workers (HCWs). Nasal cultures were obtained from 141 HCWs, 37 (26%) of whom showed S. aureus. After 72 to 96 hours of treatment, the organism was eradicated in 15 (94%) of 16 by mupirocin and in 8 (44%) of 18 by bacitracin (P = .0031). Similar efficacy was demonstrated at 30 days. Mupirocin may be more effective than bacitracin for eradication of S. aureus in healthy HCWs.

Adult↗
PubMed2004

From road rash to top allergen in a flash: bacitracin.

BACKGROUND: Bacitracin is an antibiotic that is produced by Bacillus subtilis, which is used in several types of consumer products, including cosmetics and ophthalmic and cutaneous ointments. OBJECTIVE: To call attention to the rising allergic contact dermatitis associated with bacitracin. RESULTS: Mass usage has resulted in an increasing number of clinically relevant allergic contact dermatitis reactions and near fatal anaphylaxis. The North American Contact Dermatitis Group has recorded its emergence as a leading allergen and continues to monitor the ever-growing allergic reaction rates. CONCLUSION: The clinical impact, scientific evidence, and need for medical cost containment all advocate the discontinuation of routine usage of bacitracin in clean surgical wounds.

Administration, Topical↗
PubMed1976

Grouping of beta-haemolytic streptococci by using coagglutination, precipitation or bacitracin sensitivity.

This study was made to compare coagglutination to precipitin test in grouping beta-haemolytic streptococci from clinical specimens and to investigate the accuracy of the bacitracin test in identification of group A streptococci. Results of grouping 126 strains with coagglutination and precipitation were identical in all except two cases. These two strains were nongroupable with precipitation but appeared as group B and C by coagglutination. When the distribution of group A, B, C and G streptococci in various clinical sources was investigated it was found that group B strains were the most frequently (41 per cent) isolated streptococci and even in isolates from pharyngeal swabs their proportion was 33 per cent. The accuracy of the bacitracin test in identification of group A streptococci was unsatisfactory as 26/62 (42 per cent) strains reported as group A by using this test were in fact group B, C or G streptococci. One of the reasons for this high number of false positives appeared to be the medium used for the preparation of the blood agar plates. In view of the frequent occurrence of non-A-streptococci in clinical specimens and high incidence of false positive in the bacitracin test it is suggested that this test should be replaced by a more efficient method of serological grouping.

Agglutination Tests↗
PubMed1978

Bacitracin production by the high-yielding mutant Bacillus licheniformis strain AL: stimulatory effect of L-leucine.

The high-yielding mutant Bacillus licheniformis AL produced only small amounts of bacitracin in the chemically defined M2 medium. L-leucine markedly stimulated bacitracin production and restored the mutant strain to its place as a superior producer as compared to Bacillus licheniformis ATCC 10716. Leucine also stimulated the growth rate of the mutant. The stimulatory effect of leucine on bacitracin production is discussed in relation to control mechanisms and overproduction of antibiotics.

Amino Acids↗
PubMed1979

Bacitracin production by whole cells immobilized in polyacrylamide gel.

Whole cells of Bacillus sp., bacitracin-producing bacteria, were immobilized in polyacrylamide gel prepared by using 5% total acrylamide (95% acrylamide monomer and 5% N, N'-methylenebis acrylamide). Bacitracin production by the immobilized whole cells was examined by using various fermentation media. In starch-bouillon medium, the initial activity of immobilized whole cells for bacitracin production was 20 to 25% that of an equivalent amount of washed cells. With successive utilizations in 1% peptone as reaction medium, activity of the immobilized whole cells increased gradually and reached a steady-state maximum having a value of 80 to 90% of the activity obtained initially with washed cells, whereas the washed cells lost most activity when utilized successively. What appeared to be growth of the bacteria in the gel was observed by electron microscopy. Therefore, the activation of immobilized whole cells during successive utilizations apparently resulted from the growth of whole cells in the gel, especially at the gel surface.

Acrylamides↗