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PubMed1989

Effect of segregation on prevention of intramammary infections by Staphylococcus aureus.

Effectiveness of segregating cows with Staphylococcus aureus intramammary infections was studied over 1 yr. Nine herds were split into control (n = 5) or segregated (n = 4) groups. Cows with S. aureus intramammary infections were milked last in segregated herds. Monthly milk samples were collected aseptically for microbiologic analysis. Mean incidences of S. aureus intramammary infections were 3.7 and 4.3 cases/100 cow-mo in segregated and control herds. The mean prevalence of S. aureus intramammary infections decreased in both segregated and control herds during the study. Mean percentages of cows with S. aureus intramammary infections at the beginning and end of the study were 33.7 and 21.5 in segregated herds and 25.3 and 15.0 in control herds. Cows in all herds with S. aureus intramammary infections were preferentially culled. There were no significant differences in incidence and prevalence of S. aureus intramammary infections between groups, suggesting that S. aureus intramammary infections can be controlled without segregation.

Animals↗
PubMed1993

Efficacy of intramammary antibiotic therapy for treatment of clinical mastitis caused by environmental pathogens.

For three California dairy herds with bulk tank SCC < 200,000/ml, twice daily milking, and no mastitis vaccine, 254 quarters with mild clinical mastitis were randomly assigned to three groups. Group A (n = 74) was treated with 62.5 mg of intramammary amoxicillin every 12 h for three milkings. Group C (n = 75) was treated with 200 mg of intramammary cephapirin every 12 h for two milkings. Group O (n = 105) was treated with 100 units of intramuscular oxytocin every 12 h for two or three milkings. Aseptic pretreatment quarter samples revealed 94 (37%) coliforms, 65 (26%) environmental streptococci, 34 (13%) other bacteria, and 61 (24%) with no isolate on bovine blood agar plates. Contagious pathogens were not isolated. Clinical cure (return of quarter and milk to normal) and bacterial cure (absence of primary pathogen isolated pretreatment) were assessed at milking 8 and d 20 after initial treatment. No difference existed in clinical (67.6, 67.7, or 66.7%) or bacterial (43.9, 55.0 or 49.1%) cure rate among groups. Clinical cure rates did not differ when quarters were grouped by etiology, but clinical cure rates for quarters with pathogens other than streptococci or coliforms were lower in group O.

Amoxicillin↗
PubMed1994

Coagulase-positive Staphylococcus intramammary infections in primiparous dairy cows.

Objectives were to determine the prevalence of coagulase-positive staphylococcal IMI in primiparous cows at first parturition, to contrast the differences in coagulase-positive staphylococcal IMI in primiparous cows at parturition in herds with high and low prevalences of coagulase-positive staphylococcal IMI in the lactating herd, and to determine the percentage of primiparous cows having persistent coagulase-positive staphylococcal IMI. Milk samples were collected aseptically from cows at the start and end of the study, at dry-off, and at parturition. Herds (n = 18) were split evenly into two categories: high (> 10%) or low (< 5%) prevalence of coagulase-positive staphylococcal IMI. At the start, the mean prevalence of coagulase-positive staphylococcal IMI in high prevalence herds was 30%, ranging from 13 to 65%, and in low prevalence herds was 2%, ranging from 0 to 5%. Overall the prevalence of coagulase-positive staphylococcal IMI in primiparous cows at parturition was 8.1% (67 of 828), ranging from 0 to 27%. Although primiparous cows from high prevalence herds had a higher prevalence of coagulase-positive staphylococcal IMI (9.2%; 40 of 436) at parturition than did primiparous cows from low herds (6.9%; 27 of 392), the difference was not significant. Of primiparous cows with coagulase-positive staphylococcal IMI at parturition, 43% had coagulase-positive staphylococcal IMI at least 2 mo after parturition. Primiparous cows with coagulase-positive staphylococcal IMI at parturition may represent significant reservoirs of infection to uninfected herdmates.

Animals↗
PubMed1994

Staphylococcus aureus colonization of teat skin as affected by postmilking teat treatment when exposed to cold and windy conditions.

Study 1 was conducted to determine whether postmilking teat treatment with ointment before exposure to cold and wind resulted in better skin health than standard teat treatment. Teat treatments tested were 1% I2 and 10% glycerin, ointment with 1% chloroxylenol, ointment with .3% 8-hydroxyquinoline sulfate, and no treatment (control). Teats were treated 7 d prior to chapping. A broth culture of Staphylococcus aureus was applied once to teats after chapping was established. Treatments were applied after milking and before sample collection for 11 d following S. aureus application. Milk samples were collected aseptically, teat skin swabbing solutions were collected, and teat condition was scored. Cows were exposed to ambient winter conditions, and a wind velocity of 152.4 m/min was applied to the mammary gland surface for 15 min immediately postmilking. Ointment and control teats had significantly better skin condition than teats treated with I2 solution. Colonization of S. aureus was greatest on ointment treated teats. Study 2 was conducted to determine whether teat condition of cows receiving postmilking I2 solution treatments would be improved if teats were blotted dry before exposure to wind and cold ambient conditions. Two mammary quarters of each cow received I2 solution treatment of study 1, but teats were blotted dry prior to exit from the milking parlor. No treatment was applied to the other teats. Teat condition scores were similar between treatments, but S. aureus colonization was significantly greater on control teats. Results indicate a possible disadvantage to treating teats with ointments after milking, as evidenced by increased S. aureus colonization.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed2001

[Clinical findings and diagnostic procedure in a dairy sheep with visna].

The goal of this report was to describe the clinical signs and diagnosis of Visna in a seven-year-old East Friesian milk sheep. A striking feature was that the ewe's behaviour changed frequently. At one time, the ewe was somnolent. A few minutes later, the sheep was alert and eating hay. The ewe was thin. It had a slight head tilt and a severe generalised ataxia. Based on the neurological symptoms and chronic weight loss, a tentative diagnosis of visna was made. Serological testing for maedi-visna was positive, and the ewe was euthanised. A postmortem examination was performed, and lung and brain samples were collected aseptically. Cell cultures from these organs were positive for viral enzymatic reverse transcriptase and for maedi-visna RNA.

Animals↗
PubMed2000

[The relationship between postburn gene expression of modulators in gut associated lymph tissue and the change in IgA plasma cells].

OBJECTIVE: To investigate the relationship between the postburn change in IgA plasma cells and those of IL- 4 and IL-6 in gut associated lymph tissue (GALT). METHODS: One hundred and twenty -- five SPF mice were enrolled in the study and randomly divided into three groups -- normal control (A), microbial inoculation (B) and microbial inoculation followed by burn injury. (C) In groups Band C, the mice were gavaged with candida albicans, and the mice in group C, were inflicted with 20% TBSA III degree burn at the 14th day after inoculation. The mice were sacrificed thereafter on 1, 2 and 3 postburn days and the samples were obtained aseptically. The number of candida albicans adhering to intestinal mucosal membrane was counted. The number of IgA plasma cells in lamina propria was determined by immunohistochemical staining. The gene expression of IL-4 in Peyer patch was detected by dothybridization and that of IL-6 in lamina propria was determined by insitu hybridization. RESULTS: (1) The postburn gene expression of IL-4 in Peyer patch decreased, but the IL-6 level in intestinal lamina propria increased. (2) The number of IgA plasma cells in lamina propria decreased significantly, but the adherence of candida albicans to intestinal mucous membrane increased obviously. CONCLUSION: The decrement of postburn gene expression of IL-4 in Peyer patch might lead to IgA plasma cell decrease, resulting in the increase of candida albicans adhering to intestinal mucosal membrane.

Animals↗
PubMed1996

Intramuscular administration of ceftiofur sodium versus intramammary infusion of penicillin/novobiocin for treatment of Streptococcus agalactiae mastitis in dairy cows.

OBJECTIVE: To determine the efficacy of intramuscular administration of ceftiofur sodium as treatment for intramammary infections attributable to Streptococcus agalactiae, compared with that for a standard treatment of intramammary infusion of penicillin/novobiocin. DESIGN: Prospective, randomized, controlled trial. ANIMALS: 72 lactating Holstein cows with intramammary infections caused by S agalactiae from 5 commercial dairies in Michigan. PROCEDURE: In 36 of 72 infected cows, ceftiofur was administered (2.2 mg/kg of body weight, IM, q 24 h) for 5 days; 150 mg of novobiocin and 100,000 U of procaine penicillin G was infused daily into each mammary gland of the other 36 cows for 2 days. Milk samples were collected aseptically at approximately 4 and 8 weeks after initial treatment. If cows were determined to be infected at 4 weeks after initial treatment, the treatment was repeated. RESULTS: The cure rate at 4 weeks (91.7%) and at 8 weeks (96.8%) after initial treatment for the penicillin/novobiocin-treated cows was significantly (P < 0.0001) higher, compared with that of the ceftiofur-treated cows (2.8 and 9.1%, respectively). Somatic cell counts were significantly (P < 0.0001) lower in the penicillin/novobiocin-treated group after treatment. CLINICAL IMPLICATIONS: Intramuscular administration of ceftiofur is not efficacious as a treatment to eliminate intramammary infections caused by S agalactiae and should not be used to reduce the prevalence of this organism in dairy herds.

Animals↗
PubMed1996

Evaluation of a coagulase-negative variant of Staphylococcus aureus as a cause of intramammary infections in a herd of dairy cattle.

A coagulase-negative variant of Staphylococcus aureus was identified in a herd of 250 lactating dairy cows. During testing of the entire herd, this strain of S aureus was isolated from aseptically collected milk samples of 25 cows. Cows with intramammary infections attributable to coagulase-negative S aureus had an increased somatic cell count in their milk, which was indicative of mastitis infection. Speciation of the Staphylococcus organisms was made, using a series of biochemical tests. A strain of a coagulase-positive S aureus also caused intramammary infections in the herd and shared identical biochemical characteristics with the coagulase-negative strain. Moreover, both strains could not be typed by the use of the International Set of Bovine Phages. Analysis of these findings indicated that a coagulase-negative variant of S aureus can cause intramammary infections in cattle, coagulase-negative variants of S aureus that cause mastitis can be more prevalent in herds than coagulase-positive variants, and clinicians should avoid misclassifying coagulase-negative S aureus as organisms that are clinically unimportant.

Animals↗
PubMed1995

A bacteriologic aid in the differential diagnosis of periapical and periodontal abscesses.

The aim of the present investigation was to determine whether spirochaetal count could help differentiate between periodontal and periapical abscesses. 23 draining abscesses were included, where 15 were diagnosed as periodontal and 8 as periapical, based on clinical and radiographic data. Exudate samples were collected aseptically and examined by both darkfield microscopy and Gram-stained smear. The results revealed a statistically high increase in spirochaetal count in periodontal abscesses compared to periapical ones. The obvious difference appeared adequate to differentiate both lesions and establish a rapid chairside diagnostic aid.

Colony Count, Microbial↗
PubMed1996

Sensitivity and specificity of mu-capture ELISA for detection of enterovirus IgM.

The sensitivity and specificity of an in-house mu-capture enzyme linked immunosorbent assay (ELISA) for enterovirus IgM in routine use was determined by analysing the results of 77 serum samples from 55 enterovirus culture-positive patients with aseptic meningitis and single serum samples from 140 patients with other infections. In addition, sera from 10 laboratory staff pre- and post-polio virus vaccination and 20 rheumatoid factor positive sera were tested for specificity. On testing the first serum specimen received, only 21 of 55 patients (38%) with aseptic meningitis yielded a positive result, rising to 33 of 55 (60%) on testing a second sample, where available. Out of 14 patients from whom multiple serum samples were tested and negative results obtained with the first serum, 12 were positive with the second sample (86%). Only patients with acute hepatitis A produced a significant number of false positives by the enterovirus ELISA (12 out of 20), but the reverse was not true: patients with enterovirus IgM did not produce false positive results in tests for hepatitis A IgM. Excluding samples positive for hepatitis A IgM, the number of non-enterovirus infections correctly reported as negative was 118 out of 120--a specificity of 98%. This test is probably the most useful serological test available at present for diagnosing recent enterovirus infection, although the limited sensitivity needs to be borne in mind.

Adolescent↗
PubMed2003

Laboratory diagnosis of enteroviral infections of the central nervous system by using a nested RT-polymerase chain reaction (PCR) assay.

Enteroviruses are the most common pathogens identified in infants hospitalized for suspected aseptic meningitis. Rapid detection of enterovirus infection is essential in taking the decision for treatment with antiviral agents and applying infection control measures in hospitalized pediatric patients. The purpose of this study was to compare the results of conventional virus isolation with those of enteroviral RNA detection by reverse transcription (RT)-PCR method in identical specimens from cases of suspected aseptic meningitis. Cerebrospinal fluid (CSF) samples were collected for viral examination from 68 pediatric patients with suspected aseptic meningitis from 1999 to 2002. These samples were inoculated in HeLa, Hep-2 and RD cell culture. The viral RNA was investigated by in-house RT-PCR method. The isolated viruses were typed by neutralization test. 36 of the 68 specimens were detected to be enterovirus positive by culture method, while 43 of them yielded positive results when RT-PCR method is used. Discrepancies occurred between the two methods in 15 specimens. While 11 specimens were positive by RT-PCR, these are found to be culture-negative. The isolated viruses were typed as Echovirus 30 (n: 30), Group B coxsackievirus (n: 5) and one isolate could not be typed by neutralization. Because of higher sensitivity and rapidity of RT-PCR, it is superior (p = 0.016) to virus culture of CSF for the diagnosis of enterovirus meningitis. Although the clinical usefulness of viral culture from CSF is limited, the final laboratory identification needs cultural techniques.

Adolescent↗
PubMed2005

Structure and secretory activity of cultured chondrocytes from patients with osteoarthritis.

Cartilage samples were taken from OA patients in order to describe and quantify pro-inflammatory mediators. Samples were cultured under aseptic conditions in Dulbecco's modified Eagle medium at 37 degrees C for 10 days. Control samples, taken from non-inflammatory cartilage, were cultured under the same conditions. The levels of NO(-)2 and NO(-)3 were measured in the supernatant using a spectrophotometric assay. The activity of MMP-1 was quantified by ELISA. The concentration of NO(-)x was 47.3 +/- 4.1 microM in the OA cartilague and 10.7 +/- 1.8 microM in the controls. The average MMP-1 activity was 3,650 +/- 387 ng/ml in the OA cartilage and 2,150 +/- 190 ng/ml in the control samples. These increased values of MMP-1 and NO(-)x observed in the OA cartilage suggest a higher catabolic activity. A morphological analysis of OA chondral tissue using light microscopy shows that the surface of the tissue is characterized by the presence of aggregated chondrocytes or "clones" but in the deeper areas isolated cells are found. These results could be a significant contribution towards the identification of biological markers indicating the presence of OA activity.

Cartilage, Articular↗
PubMed2002

Bacteria in heart and lungs of young chicks.

AIMS: The purpose of these experiments was to determine whether the heart and lungs of young chicks harboured bacteria. METHODS AND RESULTS: Samples of the heart and lungs were aseptically removed from chicks on scheduled sampling days. Experiment 1 showed that of the 360 birds evaluated during the late embryonic and early post-hatching periods, only 10.8% harboured bacteria in the heart, lungs, and heart and lungs simultaneously. Experiment 2 suggested that bacteria in these organs were transient. Twenty-three bacterial species were found in the hearts whereas 30 were found in the lungs. Experiment 3 showed that only 1.4% of embryos harboured bacteria in the yolk, albumen, heart and lungs whereas 12.9% of the embryos had bacteria in the air cell. CONCLUSIONS: During the post-hatching period, there was a higher incidence of bacterial isolation in the heart and lungs, whilst during the embryonic development period, there was a lower incidence of bacterial isolation from these two organs. Results suggested that the heart and lungs do not have a residual bacterial flora; rather, opportunistic bacteria occasionally pass through these tissues. SIGNIFICANCE AND IMPACT OF THE STUDY: These experiments proved that bacteria could be isolated in the heart and lungs of healthy chicks reared from E17 to 3 weeks of age.

Animals↗
PubMed2004

Increasing resistance against antibiotics in bacteria isolated from the lower urinary tract of an outpatient population of spinal cord injury patients.

OBJECTIVE: To investigate changes of the bacterial spectrum and susceptibility in bacteria isolated from urine samples of spinal cord injury patients followed in a strict outpatient setting. SUBJECTS AND METHODS: Due to neurogenic dysfunction, urinary tract infections are common in spinal cord injury patients. Nosocomial urinary tract infections and resistance against antibiotics are increasing problems in hospitalized spinal cord injury patients. Urine samples were obtained by aseptic catheterization during 1,293 outpatient appointments at our institution over a period of 6 years. The urine samples were analyzed for bacterial colonization and microbiologically evaluated. RESULTS: We demonstrate significant changes in both bacterial spectrum and bacterial resistance in an outpatient population as well. Even multiresistant staphylococcus species were detected, in spite of excluding nosocomial infections. CONCLUSIONS: Antibiotic treatment should be limited to symptomatic urinary tract infections and be initiated after sensitivity testing only. Empiric use of antibiotics must be limited to highly symptomatic infections until the results of sensitivity testing are available.

Ambulatory Care↗
PubMed2006

A comparative study of the incidence of aseptic meningitis in symptomatic natural mumps patients and monovalent mumps vaccine recipients in Japan.

To compare the incidence of aseptic meningitis associated with symptomatic natural mumps infection and in mumps vaccine recipients, we conducted a prospective comparative study. Consecutive samples of 1051 children with mumps were enrolled by 10 pediatricians and 21,465 vaccine recipients by 143 pediatric primary care practitioners, from January 1, 2000 to January 1, 2003. Parents used a daily diary to record symptoms during the period of illness (15 days) or 30-day period following immunization. Mumps infection was confirmed by virus isolation and/or detection of mumps virus genome in salivary and CSF samples. The incidence of aseptic meningitis was 13/1051 (1.24%) in patients with symptomatic natural mumps infection and was estimated to be 0.7-1.1% of overall infection in considering asymptomatic infection, and 10/21,465 (0.05%) in vaccine recipients. Although aseptic meningitis is a clear side effect of the mumps vaccine, the incidence is considerably lower than among those with symptomatic natural infection. Our results provide an informative data for consideration to resume mumps vaccine as a part of routine immunization schedule for Japanese children.

Adolescent↗
PubMed1996

An examination of nurses' practices when performing aseptic technique for wound dressings.

The purpose of this study was to establish if nurses' actions when carrying out 'aseptic technique' using the 'gloves technique' are simple and based on up-to-date knowledge and do not incur unnecessary wastage. A sample of convenience was used involving 21 trained nurses. Observation and formal interviews were used to collect quantitative and qualitative data. Results showed that not all nurses in the sample applied a 'simple aseptic technique'. The rationale for the practice of aseptic technique was not always research based, though other aspects of wound management were derived from research findings. The study highlighted other areas of aseptic technique which require investigations.

Asepsis↗
PubMed2004

Assessment of the California mastitis test usage in smallholder dairy herds and risk of violative antimicrobial residues.

This study evaluated how predictive the California Mastitis Test (CMT) is for sub-clinical mastitis under tropical smallholder dairy production conditions in Kenya. It intended to establish whether the CMT usage could be contributing to misdiagnosis and consequent mistreatment with animal drugs resulting in residue problems. Milk samples (n = 239) were aseptically collected from lactating cows in the Rift Valley of Kenya and tested using the CMT, somatic cell counts (SCC) and bacterial culture. The samples were also screened for violative drug residues using the commercial delvo test and compared to the milks mastitic status for possible association. There was a numerical but non-significant (p > 0.05) difference evident in the frequencies observed using the three different mastitis indicators. The prevalent bacterial species isolated from mammary glands with subclinical mastitis were Staphylococcus aureus (45.6%), coagulase-negative Staphylococci (13.0%), Streptococci (11.7%) and Escherichia coli 5.9%. There was an overall poor but significant (p < 0.05) correlation between the CMT and the violative antimicrobial residues in samples from all quarters, infected and non-infected respectively. The results suggest that the CMT use amongst the smallholder dairy sector as a mastitic indicator may not be a risk factor in violative antimicrobial residues problems in milk.

Animals↗
PubMedDate not supplied

Exchange nailing for aseptic tibial shaft nonunion: emphasis on the influence of a concomitant fibulotomy.

BACKGROUND: Exchange nailing is reported to have a high success rate for aseptic tibial nonunions. However, sample sizes in all series in the literature were small, and the influence of a concomitant fibulotomy was not evaluated. METHODS: Fifty-four aseptic tibial shaft nonunions for 1.2 approximately 4.3 (mean, 2.4) years were treated with exchange nailing. Indications for this technique included an aseptic nonunion of the tibial shaft with an inserted intramedullary nail and < 2 cm of shortening. A fibulotomy was performed in a nonunion with poor shaft alignment, which concomitantly required manipulation to correct. The success rate of exchange nailing was determined, and whether a concomitant fibulotomy affected the success rate was evaluated. RESULTS: Forty-seven nonunions were followed-up for 1.1 approximately 6.9 (mean, 3.8) years, and all nonunions healed. The union rate was 100% (47/47), and the average period required to achieve union was 4.7 (3.0 approximately 7.5) months. The average union periods between the fibulotomy and non-fibulotomy groups did not statistically differ (4.6 vs. 4.8 months, p = 0.61). There were no significant complications in either group. CONCLUSIONS: Exchange nailing is an excellent technique to treat aseptic nonunions of the tibia. It can be considered the treatment of choice for all indicated cases. A concomitant fibulotomy has minimal influence on the success rate. It can be performed when the shaft alignment needs correction.

Adult↗