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Post-inoculation changes in enzyme activity of Aedes aegypti infected with Chikungunya virus.

Levels of acetylcholinesterase, non-specific esterases, glutathione-S-transferase and glucose-6-phosphate dehydrogenase in Aedes aegypti (L.) mosquitoes inoculated intrathoracally with Chikungunya virus were elevated, as compared to uninoculated control insects. A number of these enzymes are important in the insects defence mechanism against xenobiotics, such as pesticides. Malathion bioassays indicated a reduction in the susceptibility of experimentally injected insects with virus or virus-free inoculum, compared to non-inoculated controls. However, insects which were mock-inoculated (injected with no inoculum) showed a similar reduction in susceptibility suggesting that the observed effect was due to the mobilization of a defence reaction in the mosquitoes in response to injury during inoculation.

Acetylcholinesterase↗

An outbreak of scabies in a school for children with learning disabilities.

An outbreak of scabies was detected in autumn 1992 at a school for children with learning disabilities. Because of the scale of the outbreak all the children at the school, and their contacts, were treated on one 'mass treatment day'. Scabies is still widely misunderstood and carries a social stigma, which makes investigation and control more difficult than it would otherwise be.

Age Factors↗

Therapy of scabies: nursing homes, hospitals, and the homeless.

Scabies epidemics in nursing homes, acute and chronic care hospitals, residential facilities, and communities have become commonplace. Patients with keratotic (crusted, Norwegian) scabies are highly contagious and often initiate outbreaks in institutions. This is a review of problems associated with the management of scabies epidemics in health care facilities. A model treatment plan, readily customized to suit specific epidemic scenarios, is detailed.

Clinical Protocols↗

[The insecticidal activity of the preparation K-othrin in relation to different species of arthropods].

A high insecticidal activity of K-otrin (flow), a preparation containing 2.5% of deltametrin, towards various arthropods, was revealed in laboratory experiments and in field trials. The effective doses were determined: 0.01-0.1 g/m2. Residual effects of the insecticide on the treated surfaces persist for 3 months and longer. Toxicologic studies revealed the possibility of using K-otrin for medical disinfection. The preparation is permitted for use by the Ministry of Health.

Animals↗

Scabies: diagnosis and treatment.

Scabies is a common, severely pruritic, contagious disorder. Treatment must be careful and methodical to avoid continuing infestation. Patients and their contacts must be treated, and clothing changed. Failure to discover all the contacts leads to continuing spread of the disease.

Contact Tracing↗

[Effect of water contamination with surface active substances and plant protecting agents on aquatic organisms].

The purpose of the study was establishing in what degree the presence of surface-active substances affects the influence of herbicides present in surface waters on aquatic organisms. The study was carried out under laboratory conditions using as herbicides promethrin (triazine group) and dichlorophenoxy-acetate sodium (2,4 D), and detergents - sodium alkylsuphonate ABS and non-ionic detergent Rokafenol N8P7. The effect of these substances was studied on the test organisms from own cultures - Daphnia magna Straus, larvae of insects Chironomus plumosus L, and young fish Lebistes reticulatus. The study showed that promethrin was the herbicide with stronger action on the studied organisms than 2,4 D. The presence of single detergents and their mixtures increased promethrin effects by 10-13% on the testes invertebrates even in concentrations permitted in surface waters. The toxic effect of the herbicide 2,4 D was potentiated by detergents in much higher concentrations, exceeding the permitted values. Contamination of surface waters with detergents may endanger the trophic chain of aquatic biocenoses.

2,4,5-Trichlorophenoxyacetic Acid↗

In vitro control of Phthirus pubis with four pediculocides: Eurax, Elimite, Licid and Benzanil.

Phthirus pubis, Linnaeus 1758 that causes phthiriasis is a common infestation disease. However, it is commonly be acquired in jails, brothels, and other similar places. The efficacy of four insecticides commercially known as Eurax, Elimite, Licid, and Benzanil was evaluated for controlling pubic lice in vitro. The four test insecticides proved to be very effective. However, they differed in time needed for causing 100 percent mortality and they could be arranged descendingly as follows: Licid, Eurax, Elimite, and Benzanil.

Animals↗

Infant parasite rates and immunoglobulin M seroprevalence as a measure of exposure to Plasmodium falciparum during a randomized controlled trial of insecticide-treated bed nets on the Kenyan coast.

Repeated cross-sectional surveys among infants sleeping under insecticide-treated bed nets (ITBN) and contemporary control infants were used to estimate changes in Plasmodium falciparum exposure due to ITBN use on the Kenyan coast. Presence of P. falciparum parasites or total P. falciparum Immunoglobulin M (IgM) seropositivity were used independently and in combination in a constant risk catalytic conversion model to estimate the force of infection in ITBN and control communities. Such studies during infancy avoid problems of early saturation of prevalence due to high forces of infection and persistence of infection, minimize problems of self-treatment, and can be conducted among large populations covering a wide geographic area. These contrast previous parasitologic studies of ITBN among older children and the traditional entomologic studies of transmission that are logistically demanding. Our investigations demonstrated that parasite prevalence, IgM seropositivity, and the force of transmission were all significantly reduced by 50%. In addition, more infants under ITBN entered their second year of life without previous exposure to P. falciparum than control infants. These effects upon delayed acquisition of effective immunity require careful monitoring during future vector control programs using ITBN.

Age Factors↗

Influence of ground ULV droplet spectra on adulticide efficacy for Aedes taeniorhynchus.

A series of 76 field trials in a vegetated habitat with caged adult Aedes taeniorhyncus mosquitoes determined that ground ultra-low volume treatment efficiency was significantly influenced by droplet size. Small droplets (7-microns volume median diameter [VMD]) produced the lowest mosquito mortality at all test distances (100-500 ft.). A VMD of 26 microns was effective at 100-300 ft., but was not effectual at 400-500 ft. Droplets in the 15-microns range were the most effective overall, at the distances evaluated.

Aedes↗

Drugs for head lice.

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Administration, Oral↗

Modification of AOAC multiresidue method for determining synthetic pyrethroid residues in fruits, vegetables, and grains. Part III: Studies of analyte stability and method ruggedness.

The stability of 8 synthetic pyrethroids in 9 crops during storage for 90 days were studied. The 8 pyrethroid insecticides were highly persistent in the 6 grains during storage. But their stabilities in 3 kinds of fruits and vegetables were different from those in the 6 grains: Most of them were degraded. Florisil purification conditions were studied with 6 batches of Florisil from 3 countries at various extents of deactivation and amounts. The best conditions of Florisil purification found in this present research agree with those found 1 year ago. The efficiencies of acetonitrile and acetone to extract the 8 pyrethroids from 6 fruit and vegetable samples were compared. The extraction efficiency of acetone was competitive with that of acetonitrile for the 6 fruit and vegetable samples. Method performance was evaluated by 6 analysts from different areas. The ruggedness tests demonstrate further that the proposed method is simple, accurate with good precision, and suitable for multiresidue analysis of pyrethroids in various agriculture products.

Acetone↗