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[The "Young Researchers" project--the effect on scientific research and educational potential in Slovenia].

The aims and the development of the "Young Researchers" project, financed and headed by the "Research Association of Slovenia", have been described in this article. The goal of this project is to obtain new personnel resources for scientific research and developmental functions with a substantial expansion of personnel potential in research activity; a proportion of the 2000 young researchers is supposed to take part in the project by the end of 1990. They will renew the advisory personnel (researchers for renovation), and the other part, together with a defined number of already qualified researchers from research and university organizations, should be involved directly in immediate development and new production (researchers for development). The authors pointed to employment problems of new resources of young researchers (problems of administrative nature), as well as problems of qualification, and those of financial nature which appeared gradually during the implementation of the project. The actual project status has been presented after four years of its implementation, and the success of the project up to now, has been evaluated in terms of the project in general, and in medical science especially.

Research Personnel↗

[Eccentric projection for the observation of the root canals of the upper premolars].

Information on the length and the shape of the root canals is necessary in dental care. They are obtained from X-ray examination of teeth. However, it is useful to apply eccentric projection for optimization in dental radiography. We observed 135 teeth and 355 films of extracted upper premolars, and tried to establish the standard of eccentric projection for the observation of root canals of the upper premolars, on the basis of the calculations and the experiments. 1. Shapes of root canals in the upper premolars are classified into 3 types with 3 subtypes. The rate of teeth with one root was 69.6%, but the rate of teeth with two root canals was 43%. These indicate the necessity of the eccentric projection in dental radiography, although there is a limit to the ability of X-ray film for confirmation of real condition of the root canals. 2. Optimal eccentric projection angle was 20 degrees-25 degrees. There is no significant difference between the paralleling technique and bisecting technique. There is no significant difference between the mesial and distal eccentric projection. But, the mesial eccentric projection is more effective for the observation of whole root and the ease of radiographic technique than the distal eccentric projection.

Bicuspid↗

[Olivocerebellar, corticonuclear and nucleocortical projections in the "four limbs area" of the paramedian lobule in the cat: a combined electrophysiological and HRP study].

The present study examined the olivocerebellar, corticonuclear and nucleocortical projections in the medial and lateral parts of the "four limbs area" of the paramedian lobule in the cat. Each part of the area could be identified electrophysiologically on the basis of their climbing fiber input. A small volume of 20% horseradish peroxidase (HRP) was injected iontophoretically into the center of an identified area. Results obtained are as follows: 1) Labeled cells were found in the rostral one-third of the medial accessory olive (MAO) of the inferior olive following injections of HRP in the four limbs area of the paramedian lobule. 2) The central MAO projects to the medial and lateral parts of the four limbs area in the caudal folium of the rostral part of the paramedian lobule. 3) The lateral and central MAO project to the medial part of the four limbs area in the intermediate part of the paramedian lobule, and the medial and central MAO project to the lateral part of the four limbs area in the intermediate part of the paramedian lobule. 4) The posteromedial three-fifths of the ipsilateral nucleus interpositus posterior (NIP) receive the corticonuclear projections from the four limbs area. 5) The medial two-fifths of the ipsilateral NIP project to the four limbs area. No strict point-to-point reciprocal relationship was found between corticonuclear and nucleocortical projections.

Animals↗

The pontocerebellar efferents from the pontine reticular tegmental nucleus in the rabbit: differential projections to the paramedian sublobules as revealed with tracing of retrograde axonal transport of horseradish peroxidase.

Projections from the nucleus reticularis tegmenti (NRTP) pontis to the cerebellar paramedian lobule were studied using a horseradish peroxidase retrograde transport technique. The rabbit's NRTP consists of a medial principal part (the nucleus papillioformis: PLF) and smaller lateral part (the processus tegmentosus lateralis: PTL). The PLF can be subdivided into a dorsomedial part--zone A, a ventrolateral part--zone C, and a main part--zone B. Zone B is furthermore divisible into the subzones: B1, B2, and B3. In addition, some cells of zone B3 invade the fiber bundles of the medial lemniscus. Experimental data have indicated a topographically organized bilateral projection from the NRTP to the anterior, the middle and the posterior folia of the paramedian lobule with some degree of contralateral dominance. In contrast to an earlier report in cats we find that not only the ventral, but also the dorsal region of the NRTP send projections onto the paramedian lobule. The neurons sending axons to the paramedian lobule are localized mainly in the middle and to a some degree in the posterior thirds of the NRTP. Zones A, B1 and B3 project onto all of sublobules of the paramedian lobule. While zone A projects primarily onto the anterior sublobules (e, f), zone B1 emanates to the middle paramedian sublobules (c, d), Zone B3 sends fibers mainly into the posterior sublobules (a, b) and to a some degree to the middle paramedian sublobules. Zone B2 distributes a few axons exclusively to the middle folia of the paramedian lobule, while the middle part of the PTL gives rise to small bilateral projection to sublobules c and d of the paramedian lobule. Neurons of zone C send projections into the middle folia of the paramedian lobule. These findings are discussed in relationship to other morphological and physiological data on neurons of the pontine reticular tegmental nucleus.

Animals↗

Differential ascending projections to aural regions in the 60 kHz contour of the mustache bat's inferior colliculus.

The inferior colliculus of the mustache bat is similar in many respects to the inferior colliculus of more commonly studied mammals. However, the isofrequency contour devoted to processing 60 kHz, the dorsoposterior division (DPD) is greatly expanded, encompassing an area approximately equal to one-third of the central nucleus. Of particular significance is that monaural and binaural neurons are segregated in the DPD into 4 spatially distinct aural regions. In this study we exploit the great enlargement of the 60 kHz region in the central nucleus of the inferior colliculus (ICc) of the mustache bat to determine the source of ascending projections to the 4 different aural regions of the DPD. Small iontophoretic deposits of HRP were made within each of the physiologically defined aural regions, and the locations and numbers of retrogradely labeled cells in the auditory brainstem nuclei were determined. Two major features of collicular organization were found. The first is that each aural region receives a unique set of projections from a subset of lower auditory nuclei and thus is distinguished both by its neural response properties and by the pattern of ascending projections it receives. The dorsomedial EE region receives inputs primarily from the ipsilateral intermediate nucleus of the lateral lemniscus (INLL) and ventral nucleus of the lateral lemniscus (VNLL), and the contralateral ICc. In contrast, the ventrolateral EE region receives projections from the ipsilateral medial superior olivary nucleus (MSO), VNLL, and INLL. The inputs to the EI region originate primarily from the dorsal nucleus of the lateral lemniscus (DNLL) and lateral superior olivary nucleus (LSO) bilaterally and from the ipsilateral INLL. The afferents to the EO region include the contralateral cochlear nucleus, the ipsilateral VNLL and INLL and MSO. The second major organizational feature is that the binaural nuclei of the brain-stem project upon the DPD in a more restricted manner than do some of the lower monaural nuclei, such as the VNLL and INLL, which project in a more widespread manner. The unique set of projections terminating in each aural region of the DPD suggests that the neurons should have substantially different properties, even when neurons in different regions are of the same general aural type. Moreover, the elucidation of the micro-organization of the DPD provides insights into the different ways that binaural properties of DPD neurons are created by the convergence of inputs from particular subsets of lower auditory nuclei.(ABSTRACT TRUNCATED AT 400 WORDS)

Afferent Pathways↗

[Projection of the neurons of the substantia nigra and ventral field of the midbrain tegmen in the cat to the putamen].

By means of retrograde axonal transport of the horseradish peroxidase and fluorochromes in the cat, it has been stated that neurons of all the parts of the substantia nigra (SN) make projections to the putamen. These projections are organized in such a way that the rostral part of the putamen gets the projected fibers from less number of the SN parts than the caudal part. To the caudal part of this formation all parts of the SN are projected, and in the equal degree to its dorsal and ventral segments. Projections to the rostral part are sent only from two parts of the SN--compact and dorsal. To the dorsal segment of this part only axons from a small amount of the nigral neurons are sent. A small amount of neurons of the tegmental ventral field give projections to the ventral segments along the whole rostrocaudal extent of the putamen. Convergence of the SN neuronal axons in the formations of the striated body has been determined, as well as overlapping of the terminal fields in the putamen from the projective neurons of the nigral various parts. Besides in different parts of the SN discrepancy has been revealed in the neuronal populations, labelled with different stainings, that contain cells, marked with two markers, injected into the nucleus caudatus and into the putamen.

Animals↗

Spinal projections of brainstem respiratory related neurons in the cat as revealed by retrograde fluorescent markers.

By means of retrograde axonal transport of fluorescent tracers, connections between brainstem respiratory related regions and the spinal cord has been studied in the cat. Neurons at the pneumotaxic center project bilaterally (90% ipsi-, 10% contra-) to cervical and lumbar spinal cord and ipsilaterally to thoracic levels. The ventrolateral nucleus of the tractus solitarius project mainly contralaterally (85%) to cervical levels and only contralaterally to thoracic levels; no efferent projections were found to lumbar levels. The ventral respiratory group showed a great number of neurons projecting to the spinal cord especially from the nucleus retroambiguus. Both nuclei, ambiguus and retroambiguus, project mainly contralaterally (70%) to the spinal cord. The Bötzinger complex showed rather scarce bilateral projections to cervical and only ipsilateral projections to lower cervical, thoracic and lumber levels.

Amidines↗

Capital projects funding for psychiatric hospitals, 1972-1981.

Data on capital projects funding in nonfederal U.S. hospitals in the years 1972, 1977, 1978, and 1981 were analyzed to identify changes in the number, cost, and funding sources of capital projects in public and private psychiatric hospitals and to compare them with changes in public and private general hospitals. The study identified three major trends over the ten-year period: a larger decrease in the number of capital projects and a larger increase in the average cost per project in public psychiatric hospitals compared with other types of hospitals, and a greater dependence by all types of hospitals on borrowed money to fund capital projects. The author suggests that the decreased number and higher cost of capital projects in public psychiatric hospitals may be related to the hospitals' increasing reliance over the ten-year period on debt financing rather than on government funding. Because of the high costs involved in debt financing--costs unrelated to the size of the debt--these hospitals may have consolidated their building projects.

Capital Expenditures↗

The organisation of the efferent projections of the zona incerta.

The hypothesis that the six cytoarchitectonically distinct subdivisions of the zona incerta send efferent projections to different parts of the brain was tested using retrograde fluorescent tracing techniques. Injections of tracer in the superior colliculus, pontomesencephalic tegmentum, thalamus or spinal cord resulted in characteristic and distinctive patterns of labelling in the zona incerta, suggesting that the different subdivisions of the zona incerta give rise to different sets of efferent projections. Thus, injections into the deep layers of the superior colliculus resulted in retrogradely labelled neurons predominantly in the zona incerta pars ventralis, whereas injections into the pontomesencephalic tegmentum, avoiding the superior colliculus, labelled neurons mostly in the zona incerta pars dorsalis. Parafascicular thalamic injections resulted in labelled neurons in the zona incerta pars rostropolaris, pars dorsalis, pars caudalis and a few in the pars ventralis. The spinal cord injections labelled predominantly the magnocellular cells of the zona incerta. The results of the double labelling experiment also give support to the conclusion that different areas of the zona incerta give rise to largely individual sets of efferent projections. Combined injections of tracers into the superior colliculus and thalamus resulted in the labelling of two essentially independent cell groups, the tectal projecting cells being found mainly in the zona incerta pars ventralis and the thalamic projecting cells being found in the zona incerta pars rostropolaris, pars dorsalis and pars caudalis. However, a small number of double labelled cells was found. These cells, which presumably send collateral projections to both the tectum and the thalamus, were found mainly in the dorsal part of the zona incerta pars ventralis, immediately next to the pars dorsalis. In the light of these findings it would appear that the zona incerta may be organised into largely distinct subdivisions, each of which has its own set of cytoarchitectonic and efferent projection characteristics.

Animals↗

The organization of neocortical projections from the ventrolateral thalamic nucleus in the brush-tailed possum, Trichosurus vulpecula, and the problem of motor and somatic sensory convergence within the mammalian brain.

The retrograde transport of HRP was used to determine the extent and organizational details of the VL neocortical projection in Trichosurus. The major, or external, part of VL projects homotypically to the anteromedial part of the parietofrontal cortex, overlapping the VP cortical projection field extensively, but not completely. Overlap between the VL field and SmI is most extensive in the area of rear limb representation, and decreases considerably in the area of the head representation. A small internal subdivision of VL projects discontinuously to the SmI head region, and appears to be limited to portions of the cortical barrel field. The VL cortical projection is much less extensive than that of VP, but does include a small area of cortex which does not receive a VP projection. Thus VL and VP do not display congruent cortical projections. Details of the thalamocortical relationships, as determined from both anatomical and physiological studies, are compared with other mammals.

Animals↗

Demonstration of postsynaptic opioid modulation of thalamic projection neurons by the combined techniques of retrograde horseradish peroxidase and enkephalin immunocytochemistry.

Thalamic projection neurons represent a major source of nociceptive information from the dorsal horn to higher centers of the neuraxis. The synaptic relationship between thalamic projection neurons and the opioid peptide enkephalin (ENK) was examined at the light (LM) and ultrastructural (EM) level using the combined techniques of retrograde transport of horseradish peroxidase and ENK immunocytochemistry. Utilizing two different chromogens to develop the peroxidase reaction product, the two labeled neural elements could be readily distinguished at the LM level in the same tissue section. In the medullary, cervical, and lumbar levels of the dorsal horn of both the cat and monkey, at least 30% of the thalamic projection neurons in lamina I were observed at the LM level to be contacted by ENK-immunoreactive varicosities. In lamina V, approximately 50% of the thalamic projection neurons received ENK contacts. Since some neurons were not observed to receive a dense ENK innervation on their somata and proximal dendrites, these data suggest that there may be different functional types of thalamic projection neurons. At the EM level, the ENK-immunoreactive varicosities were observed to form asymmetrical synaptic contacts on the labeled somata and proximal dendrites of the projection neurons. In all cases, the ENK varicosities were morphologically similar and contained round or oval agranular vesicles and a few dense-core vesicles. These observations suggest that ENK acts to a substantial degree on postsynaptic opiate receptors located on thalamic projection neurons.

Afferent Pathways↗

The visuotectal projections made by Xenopus 'pie slice' compound eyes.

In xenopus embryos at stages 28-32 one quarter to one third of the left eye rudiment was replaced by a similarly sized piece from a different position in a right eye rudiment. Three groups of operations were performed: (1) temporal tissue was placed in a nasal position; (2) nasal tissue was placed temporally; (3) ventral tissue was placed dorsally. The visuotectal projections made by these 'pie-slice' compound eyes were assessed electrophysiologically at 1 week to 6 months after metamorphosis. Of 97 animals, 71 yielded interpretable projections. In most cases two projections could be identified in each map. One, ascribed to the host part of the retina, extended over the entire tectal surface mapped. The other, identified as that from tissue derived from the pie-slice graft, projected to the tectum in register with that part of the host retina which matched the pie-slice in origin. Both projections were well ordered, and in the orientation expected if the corresponding piece of retinal tissue had participated in a normal projection. Consistent differences in pie-slice size and tectal coverage between the three groups were found. Pie-slices of nasal origin gave maps showing that they came from a relatively large portion of the retina and projected to a relatively large amount of the tectum; those of temporal origin occupied relatively small amounts of field and tectum. It was concluded that these results are further evidence for the existence of positional markers in the retina which are used for the assembly of the retinotectal map.

Animals↗

Factors involved in the development of ipsilateral retinothalamic projections in Xenopus laevis.

The extent, and the development, of the ipsilateral retinothalamic projection in the frog Xenopus laevis have been studied using terminal degeneration and autoradiographic techniques. This ipsilateral projection derives only from those retinal areas receiving visual information from the binocular portion of the visual field. In Xenopus, the ipsilateral retinothalamic projection arises from a larger area of the retina than was found to be the case in earlier studies on Rana. This correlates with the fact that Xenopus has a larger binocular visual field than does Rana. The ipsilateral retinothalamic projection is just detectable at about stage 56 of larval life, considerably later than its contralateral counterpart. Experimental manipulation of the developing eye vesicle at early larval stages followed by histological studies of the ipsilateral retinothalamic projections showed, however, that the retinal areas which give rise to this projection are determined by stage 32 of larval life. Further studies, in which monocular enucleation was performed at different larval stages with subsequent examination of the retinothalamic projections from the remaining eye, indicated that the selective pattern of decussation and non-decussation of retinothalamic fibres at the optic chiasma does not require interactions, at the chiasma, between optic fibres from the two eyes.

Animals↗

[Projection of different ganglion cell classes in the albino rat retina. A study using horseradish peroxidase].

In order to demonstrate the central projections of the different ganglion cell classes in the albino rat retina horseradish-peroxidase was injected into the superior colliculus (CS) and into the lateral geniculate nucleus (Cgl). The study presents the following results: a) Injection into the CS: Labelled cells are only in the contralateral retina. Topistically, the retino-collicular projection is in good agreement with the findings of physiological methods. The nasal half of the retina projects mainly to the posterior part and the superior half to the lataral part of the CS. Taking into consideration our cell-size-classification among 500 labelled cells we found 63% of the small group (7...10 micrometer soma diameter), 35% of the medium-sized (11...14 micrometer) and only 2% of the large group (15...23 micrometer). The small cells (W-system)project mainly, but not alone and not exclusively to the CS. b) Injection into the Cgl: In the ipsilateral retina we found a few labelled cells in the superior, temporal region. Contralateral--as after CS-injection--only half of ganglion cells is labelled. The quantitative analysis of 500 labelled cells indicates: 32% belong to hte small group, 51% to the medium-sized and 17% to the large group. There are fibers of all three classes, but in the majority those of the medium-sized class terminating in the Cgl. It is discussed whether these results allow a comparison of the size classes with the W-, X- and Y-system. Our findings show that there is no clear agreement between cell-size and projection site as demonstrated in cats. There is a large overlapping of the cell classes with respect to their central projection. We found that with increasing soma-diameter the ganglion cells project more and more to the Cgl. It is known that in the Golgi-picture cells with different soma diameter are of the same neuron type. Therefore, a morphological comparison of the ganglion cells of the rat with the W-, X- and Y-system is only ingenious taking into consideration also the dendritic structure.

Animals↗

Chemical coding of neurons projecting to pelvic viscera in the male guinea pig: a study by retrograde transport and immunohistochemistry.

Retrograde transport studies using Fast Blue dye demonstrated that the ductus deferens, seminal vesicle, prostate and rectum, but not the urinary bladder of the male guinea pig are at least in part innervated by the anterior major pelvic ganglion. In the ductus deferens, seminal vesicle and prostate innervation is derived from ipsilateral and contralateral ganglia. In addition to retrograde studies, dye-filled neurons were analysed immunohistochemically for neuronal markers and associations with specifically identified neuronal projections. Neurons of the ganglion projecting to the ductus deferens either contained tyrosine hydroxylase alone, tyrosine hydroxylase and neuropeptide Y, neuropeptide Y alone, neuropeptide Y and vasoactive intestinal peptide, or vasoactive intestinal peptide alone. These neurons were associated with three classes of neuronal projections, substance P-, leucine-enkephalin-, and methionine-enkephalin-immunoreactive. Neurons projecting to the seminal vesicles were similar to the neurons supplying the ductus deferens, except none of the seminal vesicle-specific neurons exhibited vasoactive intestinal peptide immunoreactivity. Neurons supplying the prostate were immunoreactive for either tyrosine hydroxylase or neuropeptide Y; these neurons were infrequently associated with the three classes of varicose neuronal projections. Neurons projecting to the rectum contained neuropeptide Y and were only associated with methionine-enkephalin immunoreactive neuronal projections in one animal.

Amidines↗

The dentatorubral projection in the rabbit with emphasis on distinction from the interpositorubral connectivity: an HRP retrograde tracer study.

The projection from the lateral (dentate) cerebellar nucleus (NL) to the red nucleus in the rabbit was investigated using free horseradish peroxidase (HRP). Following injections of this tracer into the red nucleus (RN), the distribution patterns of retrogradely labeled cells were checked in the NL. The projection from the NL was found to extend rostrocaudally throughout the contralateral RN except for the predominant part of the rostral one-quarter and the caudalmost part of the RN, i.e. the regions described to receive projections exclusively from the interpositus nuclear complex (NI). On the other hand, the only region of the RN receiving projection exclusively from the NL appeared to be rostralmost parvocellular region of the RN (RN-PA). This study suggested that, in contrast to other species, the projection from the NL is not confined to the most rostrally localized RN-PA but is distributed throughout predominant part of the nucleus and to a great extent overlapped with projection from the NI. This overlapping projection pattern parallels the absence of clear differentiation between parvo- and magnocellular parts of the rabbit RN.

Afferent Pathways↗

IMASIS computer-based medical record project: dealing with the human factor.

The Institut Municipal d'Assistència Sanitària (IMAS) is a health care organization in Barcelona, comprising two general hospitals, a psychiatric hospital, a surgical clinic, a geriatric center, some primary care clinics, and a research institute. Since 1984, IMAS has been engaged in creating a multicenter integrated hospital information system (IMASIS). Currently, IMASIS offers the possibility to manage administrative data, laboratory results, pathology and cytology reports, radiology reports, and pharmacy inpatient orders; it also shares this information on-line among IMAS centers. IMASIS users may also work with a word processor, a spreadsheet, a database, or a statistical package and have access to MEDLINE. A second phase of IMASIS development began in December 1993 focused on clinical information management. The goal was to move towards an integrated multimedia medical record [1]. As a first step, the implementation experiences of the most advanced hospital information systems around the world were studied. Some of these experiences detected behavioral, cultural, and organizational factors [2] as the main sources of delay, or even failure, in HIS projects. A preliminary analysis to define such factors, assess their potential impact, and introduce adequate measures to deal with them seemed unavoidable before structuring of the project. In our approach to physician attitudes analysis, two survey techniques were applied. First, every hospital service head was contacted to schedule an interview, with either a service representative or a group of staff physicians and residents. The aim was to provide detailed information about project objectives and collect personal opinions, problems encountered in the current HIS, and specific needs of every medical and surgical specialty (including imaging needs). Every service head was asked to distribute a questionnaire among all clinicians, which assessed frequency of use of IMASIS current applications, user's satisfaction level, problems to be solved in utilization of the system, errors detected in the systems' database, and the personal interest in participating in the IMASIS project. The questionnaire was also intended to be a tool to monitor IMASIS evolution. Our study showed that medical staff had a lack of information about the current HIS, leading to a poor utilization of some system options. Another major characteristic, related to the above, was the feeling that the project would negatively affect the organization of work at the hospitals. A computer-based medical record was feared to degrade physician-patient relationship, introduce supplementary administrative burden in clinicians day-to-day work, unnecessarily slow history taking, and imply too-rigid patterns of work. The most frequent problems in using the current system could be classified into two groups: problems related to lack of agility and consistency in user interface design, and those derived from lack of a common patient identification number. Duplication of medical records was the most frequent error detected by physicians. Analysis of physicians' attitudes towards IMASIS revealed a lack of confidence globally. This was probably the consequence of two current features: a lack of complete information about IMASIS possibilities and problems faced when using the system. To deal with such factors, three types of measures have been planned. First, an effort is to be done to ensure that every physician is able to adequately use the current system and understands long-term benefits of the project. This task will be better accomplished by personal interaction between clinicians and a physician from the Informatics Department than through formal teaching of IMASIS. Secondly, a protocol for evaluating the HIS is being developed and will be systematically applied to detect both database errors and systemUs design pitfalls. Finally, the IMASIS project has to find a convenient point for starting, to offer short-term re

Attitude of Health Personnel↗

Participatory research in a Native Hawaiian community. The Wai'anae Cancer Research Project.

BACKGROUND: Community participation was a key component of a cancer control research project in a Native Hawaiian community. This project tested the effectiveness of a culturally appropriate intervention as a means of increasing breast and cervical cancer screening practices among Native Hawaiian women on the Wai'anae Coast of the island of O'ahu. METHODS: The Wai'anae Cancer Research project was community driven, with Native Hawaiian community representatives involved in all phases of the project, from grant proposal development to data interpretation. A community health center administered the grant award from the National Cancer Institute. The policymaking steering committee included community representatives, health professionals, and researchers to balance community and scientific quality standards. A factor in the project's success was continuous involvement over 7 years by a core of community representatives and professional staff. RESULTS: More than 500 women participated in the intervention, and outcome measures indicated that there was a community wide impact on cancer-related knowledge, attitudes, and behaviors. Important contributions of the project also included direct economic benefits, improvements in health services and systems, increased research capabilities, and dissemination of findings to other communities and researchers. CONCLUSION: Community participation in all phases of the research was essential in generating community acceptance and resulted in an innovative and effective intervention. This participatory research project has left the community richer in knowledge, skills, experience, confidence, and resources. These qualities provide a strong foundation for building future programs and research.

Breast Neoplasms↗