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At least 1,603 records · Page 89Linked to original sources
PubMed1999

[Analysis of the influence of temperature on the stability of sex steroids in cow feces].

Up to the present natural and synthetic steroids have only rarely been considered a cause of disturbances of sexual behavior and anomalies of sex organs. The increasing environmental contamination with chemicals showing estrogenic effects underlines the importance of further investigations in that matter. Therefore, the concentrations of estrogens and progesterone in the faeces of cattle were examined over a 13 weeks period. The values obtained are presented as equivalents of the standard substances estrone and 4-pregnene-20 beta-ol-3-one. The samples were stored in a refrigerator or incubator at temperatures of 5 degrees C and 30 degrees C, respectively, and the investigations were carried out by using an enzyme-immunoassay. Estrogen and progesterone concentrations decreased more rapidly when incubation was performed at 30 degrees C as compared to storage at 5 degrees C, and estrogen values declined more slowly than those of progesterone. When keeping the samples at 5 degrees C the estrogen concentrations decreased to below the basal values only beyond the eleventh week of storage. In contrast, comparable concentrations were already obtained during the third week of the experiment if the samples were stored at 30 degrees C. Progesterone levels decreased to below the basal values during the fourth week of storage at 5 degrees C and during the second week at 30 degrees C.

Animals↗
PubMed1998

[Well water pollution in the Khombole district: research on the contamination by organochlorine pesticide residues and organic substances (feces)].

The study realized in the district of Khombole (SENEGAL) has permit to estimate the contamination levels of wells waters used by the populations. The research and the dosage of the organichlorine pesticide residues, nitrites and nitrates and microbiologic analysis have been done on 19 wells chosen after a drawning of lots. The organochlorine pesticide residues which have been found prove that the wells are permanently exposed to these chemical substances which don't constitute nevertheless a major risk for the populations health. The results of our research proved also that there is a real risk of intoxication with the nitrogen oxides. In effects more than 50% of the wells have revealed nitrates contents up to the indicative value (25 mg/l). As for the nitrates, with a few exceptions (5/18), the contents are superior to the authorized norm (0.1 mg/l). By another way the bacteriologic analysis has revealed in the one hand a DBT (Total Bacterian Count) up to 10,000 germs/l for all the wells, and in the other hand the presence of Escherichia coli, Enterococcus faecalis that confirm a faecal contamination.

Colony Count, Microbial↗
PubMed1999

[Occurrence of Escherichia coli O157 in feces of healthy persons, from selected groups, in the country].

The purpose of the study was determination of the occurrence of E. coli O157 in faeces samples of healthy subjects and characterization of the isolated strains with respect to their potential pathogenicity. The study was carried out in two stages. In the first one in 5 sanitary-epidemiological stations samples were tested from healthy subjects after inoculation onto McConkey (MC) or/and McConkey with sorbitol (SMC) media and isolating from each culture 10 lactose-positive (on MC medium) or sorbitol-negative (on SMC) colonies. Then latex test was done with each isolate for E. coli O157 presence. In all, 1005 samples were studied, including 260 taken from children aged 0-2 years, 180 samples from children aged 3-10 years, and 565 samples from older children and adults. E. coli O157 rods were cultured from 6 adults (0.6%). In the second stage carried out at the Laboratory of Enterobacteriaceae, Bacteriology Department, National Institute of Hygiene strains obtained from territorial laboratories were studied determining their phenotypic and genotypic traits regarded as virulence markers of verotoxic E. coli O157 strains, such as inability to ferment sorbitol and MUG breakdown, and production of verotoxins and enterohaemolysin. By the PCR method fragments were sought of genes coding for production of verotoxins, intimin and enterohaemolysin. The results showed that no E. coli O157 strain obtained from healthy individuals produced verotoxins, but three studied strains contained the eae gene determining intimin production and they were regarded as enteropathogenic.

Adult↗
PubMedDate not supplied

[Electrolyte levels in feces and urine in healthy breeding calves between the ages of 5 days to 6 months].

In contrast to the situation in adult cattle there are high concentrations of sodium and low concentrations of potassium in the faeces of suckling calves. It was investigated in this study in which stage of the development of the ruminant these changes in electrolyte levels occur, and whether there are changes in electrolyte levels in urine as well. Samples of faeces and urine from healthy calves were examined fortnightly starting with the 5th day after birth. There was a distinct decrease in sodium concentration in faecal water within the first three weeks of life, i.e. without any relation to the intake of solid feed. However, the levels of sodium, potassium and chloride in urine samples and of potassium in faecal water increased with the development of rumination. The concentration of chloride in faeces was on a low level during the whole period of investigation.

Age Factors↗
PubMed2001

[High sensitivity of Escherichia coli to antimicrobial agents used for first-line treatment of urinary tract infections; results of an examination of feces of healthy subjects in Friesland].

OBJECTIVE: To determine the basic sensitivity of Escherichia coli in the province of Friesland, the Netherlands, to antimicrobial agents used by general practitioners to treat urinary tract infections. DESIGN: Inventory. METHOD: Fifty general practitioners in the province were asked in 1999 to have faeces submitted by patients who had not been using antibiotics for at least one month. E. coli was isolated from the faeces using an elective medium. The proportions of resistance were compared with those of strains isolated in urine sent for examination to Friesland Public Health Laboratory by a clinic, outpatient department, general practice or nursing home. RESULTS: The sensitivities were tested of 240 strains from 240 healthy subjects (73 males and 167 females; mean age 47 years (range 0-84)). The proportions of strains resistant to the agents tested were as follows: nitrofurantoin: 0.8%, trimethoprim: 10%, co-trimoxazole: 10%, amoxicillin: 15%, amoxicillin-clavulanic acid 0.4%. Forty-eight per cent of the strains showed intermediate susceptibility to amoxicillin, 63% to amoxicillin/clavulanic acid. The resistance was lower than in isolates submitted for examination from general and specialist practices. CONCLUSION: The antimicrobial agents mentioned are still useful for treatment of urinary tract infections in the general practice.

Adolescent↗