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Improvement of fertility and semen quality in men treated with a combination of anticongestive and antibiotic drugs.

The effects of a combined therapy of antibiotics and antiinflammatory drugs was evaluated in 344 men referred to our clinic for treatment of infertility. Physical signs of congestion, usually not severe, were detected in 244 men. The treatment caused significant improvement in semen quality, especially in sperm concentration, morphology and motility. Forty percent of the wives became pregnant. There was a striking relationship between increase in morphologically normal spermatozoa and incidence of pregnancy. It is speculated that the therapy had its major effect at the level of the epididymis and/or testis.

Adult↗

Release of E-rosette augmenting factor (E-RAF) after stimulation of human leukocytes with mitogens or antigens.

Stimulation of human peripheral blood leukocytes (HPBL) with mitogens such as phytohemagglutinin or concanavalin A increases the total number of lymphocytes that form rosettes with sheep erythrocytes. A similar effect is seen when HPBL from skin test-positive, but not skin test-negative, donors are stimulated by a specific antigen. It was also found that the culture fluids from mitogen-stimulated lymphocytes contained a substance that significantly increased the percentage of E-rosette forming cells (85 to 95%) over that of control culture fluids (40%). A similar phenomenon was also observed with supernatant fluids derived from antigenic stimulation of cells from skin test-positive donors, but not from skin test-negative subjects. The factor that produces this effect has been designated E-rosette augmenting factor (E-RAF). It is nondialyzable; it appears in the supernatants of antigen or mitogen-stimulated cells within 12 hr; and its production is not blocked by mitomycin C. It is produced by cells that do not adhere to glass wool columns and by mitogen-stimulated thymocytes. Sephadex G-100 chromatography showed that mitogen-induced E-RAF eluted from the column in advance of antigen-induced E-RAF. E-RAF has many properties that are characteristic of lymphokines.

Antigens↗

[Cell-mediated immunity after blocking of the H2 lymphocyte receptors].

The effects of cimetidine have been evaluated in 10 normal subjects on cutaneous delayed reactions in vivo, and on E rosette forming cells in vitro, in order to define the significance of the lymphocytes H2 receptors in cell-mediated immunity. The results show the immuno-suppressive activity of histamine, but do not allow us to hypothesize an immuno-regulatory action of cimetidine.

Adult↗