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[Variability of muscular tissue distribution in the hindlimb of lambs. I. Individual muscles].

Using multivariate analysis of the centered data, this paper describes the extent of variation of the weight distribution of individual muscles in the hindlimb of lambs of 18.9 +/- 3.1 kg carcass weight. We studied a sample of 52 ram lambs and 36 ewe lambs of different morphological types slaughtered at a comparable stage of body development (same relative state of carcass fattening). Variability in muscle weight was similar in the two sexes (average CV = 21.95% in ram lambs and 22.5% in ewe lambs) and changed from 14.84 to 43.91% in the former and from 14.91 to 41.93% in the latter. Some of the mass variability of the different muscles was related to the total muscle weight of the hindlimb, but a part was also due to intrinsic variation related to the nature of the muscles. The most discriminant variables of the population were of very low weight: Soleus (SO), Extensor digitorum lateralis (ED), Gemelli (GE) and Quadratus femoris (QF); moderately low weight: Sartorius (SA); or average weight: Vastus medialis (VM). Their order of importance was not the same in the two sexes. The breed groups could be distinguished by the weight ratio values of these muscles.

Animals↗

Innervation zones of the upper and lower limb muscles estimated by using multichannel surface EMG.

The distribution of innervation zones was investigated in 3 subjects for 17 muscles and 8 muscle groups in the upper and lower limb, by detecting bi-directional propagation of motor unit action potentials (MUAPs) with the multichannel surface electrode array. Clarification of the distribution of innervation zones depended on the ease in detecting the propagation of MUAPs and the actual scattering of innervation zones, which were closely related with muscle morphology with respect to the arrangements of muscle fibers. In muscles having fibers running parallel to each other, such as the biceps brachii, intrinsic hand muscles, vastus lateralis and medialis, tensor fasciae latae, peronei, soleus, tibialis anterior, and hypothenar muscles in the foot, it was relatively easy to detect the propagating MUAPs, and the innervation zones were distributed in a relatively narrow band around muscle belly. On the other hand, in muscles with a complicated structure including pinnation of muscle fibers, in-series muscle fibers and aponeurotic tissues, such as the deltoid, flexors and extensors in the forearm, rectus femoris, sartorius, hamstrings and gastrocnemius, it was more difficult to detect the propagating MUAPs and to identify the innervation zones, which were widely scattered or distributed in complex configurations. The distribution of the innervation zones clarified in the present study can be used to find the optimal location of electrodes in surface EMG recordings and of stimulus electrodes in the functional and therapeutic electrical stimulations. It may also be useful in motor point biopsy for diagnosis of neuromuscular diseases as well as in the botulinum toxin injection for the treatment of spasticity.

Action Potentials↗

[Comparative characteristics of electrophysiologic changes in frog muscle fibers after denervation and following blockade of axoplasmic transport].

Using anatomical denervation and a single application of colchicine the k ACh sensitivity and electric features of the membrane of the m. sartorius fibers were studied. Within 2 weeks after anatomical denervation a similar enlargement of the area sensitive to ACh occurred as well as a decrease in the MP values, in effective impedance, and in time constant of the muscular membrane. These data suggest that the postdenervation changes in the frog muscle are due mainly to cessation of the substances inflow with the fast axoplasmatic transport. Two latter parameters change at that more rapidly after colchicin which suggests a greater effect of the deficit of trophic substances transported along the axons, on the functioning muscle (colchicine) than on the inactive (denervation).

Animals↗

Efferent and afferent activity in a gastrocnemius nerve branch during locomotion in the thalamic cat.

The firing patterns of alpha and gamma efferent fibres and of group I and group II afferent fibres innervating the gastrocnemius muscle were observed during spontaneous locomotor movements in the thalamic cat. Multi-unit discharges of each kind of fibre were obtained by electronic sorting of the action potentials from the whole activity of a thin branch of gastrocnemius lateralis or medialis nerve. The main results were: During the locomotor cycle the activity of the afferent and efferent populations was highly modulated. alpha- and gamma-motoneurones were co-activated within the locomotor cycle during ankle plantar-flexion. The gamma discharge began to rise earlier and to fall later than did the alpha discharge. The amplitude of the gamma discharge, unlike that of the alpha discharge, was largely independent of the vigour of walking. Between the cyclic discharges, most of gamma populations were tonically active whereas alpha populations were silent. Subgroups of the alpha and gamma populations were not usually activated according to the cell-size principle, but, the activation of the latest gamma subgroup always preceded that of the earliest alpha subgroup. Modulation of the group I and II afferent discharges was closely related to the cyclic length changes of the parent muscle. Fusimotor activation during the active shortening of gastrocnemius muscle prevented the afferent discharges from pausing. The pattern of afferent and efferent activity during selective curarisation of the extrafusal junctions indicated that the discharge of static gamma-motoneurones is modulated during the locomotor cycle. After curarisation of both extrafusal and intrafusal junctions, an efferent-discharge pattern of central origin persisted alternately in extensor- and flexor-muscle nerves (fictive locomotion). The durations of the fictive locomotor cycle and of the cyclic discharge in the sartorius nerve were increased as a consequence of the suppression of phasic afferent inputs to the C.N.S. Maintained ankle dorsi-flexion slowed the fictive locomotor rhythm and elicited opposite effects, respectively excitation and depression, on the magnitude of the alpha and gamma discharges. Maintained ankle plantar-flexion scarcely perturbed the duration of the fictive locomotor cycle, but the duration of the sartorius-nerve discharge lengthened at the expense of that of the gastrocnemius discharge. Both gastrocnemius alpha- and gamma-motoneurones were depressed, the former considerably more than the latter. The roles of the gastrocnemius afferents and gamma-efferents during the locomotor cycle are discussed in the light of these results.

Animals↗

Effects of bilateral lesions of the dorsolateral funiculi and dorsal columns at the level of the low thoracic spinal cord on the control of locomotion in the adult cat. I. Treadmill walking.

1. A quantitative and longitudinal analysis of locomotion was made after bilateral lesions of the dorsolateral funiculi (DLF) and/or the dorsal columns (DC) in the lower thoracic cord (T12 or T13) in five adult cats. All cats were chronically implanted several weeks before the spinal cord lesion to permit the recording of electromyographic (EMG) activity from selected flexor and extensor muscles of the fore- and hindlimbs of each side. This allowed each cat to act as its own control when comparing the pattern and amplitude of EMG activity before and after the lesion. All experiments were also videotaped to allow an analysis of the kinematic changes before and after the lesions. Kinematic data were only analyzed for the side of the cat facing the camera; for all cats this was the left side. 2. After recovery periods of 2-5 mo, wheat-germ-agglutinated horseradish peroxidase (WGA-HRP) was injected caudal to the lesion site (normally at L2). The extent of the lesion was verified both histologically and by evaluating the number of HRP-labeled neurons in different supraspinal structures. These analyses showed that the cortico- and rubrospinal tracts (CST and RST, respectively) were completely interrupted, bilaterally, in two of five of the cats; in one of these cats the DCs were also interrupted. In the other three cats there was more variable damage, and the CST and RST were only completely interrupted on the right side of one of these animals. The DCs were completely sectioned in two of these cats. 3. During the 1st wk subsequent to the lesion, most cats had difficulty in supporting their weight and in walking. However, within 10 days all were able to walk, unaided, for extended periods on the treadmill at speeds of at least 0.35 m/s. In the two cats with the complete, bilateral DLF lesions, the animals dragged both their left and right hindpaws along the treadmill belt during the swing phase of the step cycle (paw drag) throughout the testing period of 3-5 mo. In the other three cats, paw drag in either hindlimb was only seen in the 1st 2-3 wk after the lesions, with the exception of the cat with the complete lesion of the DLF on the right side, which showed sustained paw drag in the right hindlimb throughout the testing period. 4. Significant increases in step cycle and swing duration following the lesion were observed only in the two cats with the largest lesions. In all five cats, statistical comparisons of the slopes from a linear regression analysis showed that the relationship between swing and step cycle duration was unchanged by the lesions. 5. Joint angles in the left hindlimb of the two cats with the largest lesions were generally smaller (more flexed) than in the prelesion controls. This was particularly true for the knee and ankle joints. The other three cats showed changed joint angle values for the hip, knee, and ankle only in the 1st 2-3 wk after the lesions. All of the cats, except the one with the least damage to the left DLF, exhibited increased joint excursions at the metatarsophalangeal (MTP) joint of the left limb throughout the recovery period. 6. There were sustained changes in the coupling between the hip and the knee of the left limb, together with smaller changes in the coupling of the knee and ankle in the two cats with the complete lesions of the DLF. In contrast to the prelesion controls, flexion at the ankle occurred before swing onset in these same two cats. The changes in the coordination between the hip and the knee were associated with changes in the temporal coupling between the hip flexor, sartorius (Srt), and the knee flexor, semitendinosus (St). Despite some improvement, the coupling between these two muscles never fully recovered to the prelesion values. Similar, but smaller, changes in the delay between Srt and St were also seen in two of the other three cats. (ABSTRACT TRUNCATED)

Animals↗

Strategies of muscular support of varus and valgus isometric loads at the human knee.

In this paper we studied how subjects activate their muscles in response to static varus and valgus loads at the knee. The muscles' contributions to the external moments were estimated using an EMG driven biomechanical model of the knee. The individual muscle activation and loading patterns were examined to identify the strategies that the nervous system uses to support varus and valgus knee moments. It was found that the (1) co-contraction of the hamstrings and quadriceps, and (2) activation of the gracilis and tensor fascia lata increased with the increasing magnitude of the varus and valgus moments. These 2 activation patterns provided positive support of valgus and varus loads at the knee The sartorius appears to be activated to provide positive support of valgus loads at the knee, whereas during varus moments this muscle increases the varus load on the knee, i.e. provides negative support. Generally, the hamstrings and quadriceps co-contraction contributed to most of the muscular support of the varus and valgus moments. In addition, co-contraction supported 11-14% of the external moment in pure varus and pure valgus respectively. It appears that there are activation strategies with the specific purpose to support varus and valgus moments, albeit small, which suggest dual goals of the neuromotor system during the support of varus and valgus moments.

Adult↗

Neuromuscular blocking effects of an alkaloidal extract from Inula royleana: contractile and electrical studies on amphibian skeletal muscle in vitro.

The neuromuscular blocking properties of an alkaloidal extract from the root of Inula royleana have been investigated in vitro using a combination of mechanical and electrophysiological approaches. Neurogenic twitches of the frog sartorius were profoundly inhibited by concentrations of the extract > or = 20 micrograms/ml, being reduced to 50% of control amplitude in approximately 90 s at a concentration of > or = 20 micrograms/ml. They were partially reversed by neostigmine (6 micrograms/ml), and by prolonged washout of the extract. Muscle surface action potentials, recorded with extracellular electrodes, also declined rapidly in amplitude in the presence of the extract. Direct muscle stimulation during inhibition by the extract elicited contractions and action potentials whose magnitudes were similar to control responses. Resting membrane potentials, and the intracellular input impedance of the skeletal muscle cells, were not significantly changed by the alkaloids. These results indicate that the extract has significant neuromuscular blocking activity of a partially or slowly reversible nature. The block appears to be exerted at the postjunctional end-plate nicotine receptors, thus offering promise for the identification of novel cholinergic receptor antagonist(s).

Action Potentials↗

[Age and changes in the fascial compartment structures in the coxofemoral joint region].

Investigations performed on 66 corpses beginning from 5-month-old embryos up to 75 years of age by methods of anatomical preparations (sections were studied after N. L. Pirogov) and on total histotopographical preparations revealed anteromedial, anterolateral and posteroinferior fascial nodes in the area of the coxofemoral joint. The anteromedial node is formed by the network that consists of connective tissue fibres of fascial cases covering the lumboinguinal and pectinate muscles and the common sheaths for the artery and vein. The anterolateral node is formed by the network that consists of the connective tissue fibres of the rectal muscle case, the sheaths of the lateral vessels circumflexing the femur and fasciae of the musculus sartorius and m. tensor fasciae femoris. The posteroinferior node is formed by the network that consists of the connective tissue fibres of the fascial cases of the m. obturatorius, internal and external, fascial sheaths of the internal pudendal vessels and the ischiadic nerve. The fascial nodes in question morphologically complete their formation by the end of the fetal period. In young and adolescent age their components have abundant fibroblastic cells, collagenous and elastic fibres are correctly oriented. By 20 years of development, the fascial nodes are dense and their supporting-protective role is clearly evident. After 55 years of age, the fascial nodes are subjected to involutional alterations characterized by decreasing number of cells, rough collagenous fibre fasciculi, transformed elastic fibres. All these signs demonstrate that their protective and supporting functions decrease at this age.

Adolescent↗

Iliac crest bone-muscle transfer in periacetabular osteotomy.

Ganz et al reported acetabular osteotomy for extensive acetabular reorientation without changing the acetabular diameter. This article reports our modification of this surgical technique, which preserves the integrity of the tensor fascia latea muscle as well as providing viable bone for bone graft at the anterior iliac osteotomy site. The interval between the sartorius and tensor fascia lata is developed distal to the anterosuperior iliac spine. After iliac osteotomy through the anterosuperior iliac spine, the distal portion of the anterosuperior iliac spine (acetabular segment), along with its slip of tensor fascia lata (1-2 cm), is separated and shaped to fit the anterior bone gap. Two small holes are drilled into that segment and also into the distal cut surface in the anterior portion of the remaining crest so that when the sutures are tightened, the tensor muscle is preserved anatomically and bone grafting is achieved.

Acetabulum↗

Synaptic effects in lumbar alpha-motoneurones evoked from group II muscle afferents via two different interneuronal pathways in the cat.

Conditioning of monosynaptic reflexes was used to investigate group II excitation from quadriceps (Q) and sartorius (Sart) in posterior biceps-semitendinosus (PBSt) motoneurones and different lesions were made to analyze the interneuronal pathways. The purpose of the investigation was to find if the excitation from group II Q and Sart afferents, which enter the spinal cord in L4-L6, is relayed to motoneurones by interneurones located in these segments and/or in the same segments (L7-S1) as the PBSt motoneurones. In some experiments a transection of the dorsal column in L6, which interrupts the group II input from Sart and Q to interneurones in the L7-S1 segments, resulted in a marked decrease (by about 2/3) of group II excitation from these nerves. In other experiments the same lesion did not reduce the group II effect. In the latter cases the effect could be abolished by a second lesion of the lateral funiculus (LF) in L5-L6. It is postulated that potent effects from Q and Sart group II afferents can be evoked by interneurones located in L7-S1, i.e. in the same segments as the PBSt motoneurones (L7-S1), and/or via interneurones in more rostral segments. The axons of the more rostrally located group II interneurones appear to descend mainly in the middle part of the LF.

Animals↗

[The diagnostic value of magnetic resonance imaging on neuromuscular diseases].

The article concentrated on the study of skeletal muscles of four extremities in 12 cases of different kinds of neuromuscular diseases and 4 volunteers with MRI. The results revealed: MRI could clearly display individual muscle, muscle groups or abnormal muscles morphologically. It could also demonstrate the difference between the neurogenic and myogenic changes. In patients with progressive muscular dystrophy (PMD), the gracilis m. and sartorius m. were relatively intact. In patients with primary myopathies, the severity of MRI changes was consistent with the severity of the illness and the EMG changes. The results indicated that MRI examination was helpful in the diagnosis of neuromuscular disorders. It can define the distribution and the severity of impaired muscles. In addition, MRI could be used to select the appropriate muscles for EMG examinations or muscle biopsies. Selective muscular impairments might be the characteristics of patients with PMD.

Adolescent↗

The clinical features of early bleeding into the muscles of the lower limb in severe haemophiliacs.

The clinical features, management and outcome of 178 early bleeding episodes into the musculature of the thigh and lower limb of 37 severe haemophiliacs are reported. Ninety-five per cent of all bleeds were treated in under three hours from onset of symptoms and the mean time to complete restoration of function was 3.5 days. The most frequent site of bleeding was the quadriceps (44 per cent) followed by the calf (35 per cent), anterior tibial compartment (seven per cent), adductors of the thigh (seven per cent), hamstrings (six per cent) and sartorius (one per cent). Bleeds of the quadriceps took longest to resolve (mean, four days), significantly longer than bleeds of the calf muscle (3.1 days). The first symptom in 66 per cent of bleeds was pain on movement. When the quadriceps was involved, this rapidly progressed to pain at rest. There was a significant prolongation of recovery time when bleeds of this muscle group were treated more than two hours after the onset of symptoms. Bleeds of the calf muscle required less transfusions and no prolongation in recovery time occurred in bleeds treated up to three hours from the onset of symptoms. The results of this study contrast markedly with earlier reports based on later presentations.

Blood Transfusion↗