[Vitamins in the immune response].
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Preliminary to a stimulant comparison study, 31 children with minimal brain dysfunction randomly received either placebo or a megavitamin combination. During a two-week trial, only two children responded so well that stiumlants were not considered necessary; both were in the placebo group. Change scores from pretest to posttest on four blind ratings by teachers and parents did not show a significant difference between the placebo and vitamin groups.
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Samples of hens' eggs produced throughout South Africa during the winter and summer of 1990 were analysed for nutrient content to obtain representative values for inclusion in food tables intended for local use. Proximate analyses and vitamin, mineral, fatty acid and amino acid determinations were carried out on all the samples. When the analysed values obtained for whole eggs, egg yolks and egg whites were compared with values listed in the Research Institute for Nutritional Diseases food tables, a number of differences were found. With regard to the inclusion of eggs in diets in general, the cholesterol content of whole eggs was found to be 23.5% lower and the total fat and saturated fatty acid contents 9% and 10% lower, respectively, than the listed values. The eggs were found to be rich sources of protein of animal origin, all the essential amino acids, thiamine, riboflavin, pantothenic and folic acids, vitamin B12, biotin, vitamin D3, vitamin E and phosphorus. Eggs are readily available and inexpensive and should be included in the diets of young children, adolescents, pregnant and breastfeeding women, adults, the elderly and particularly those of all ages who are undernourished.
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Pantothenate (vitamin B5) is a water-soluble vitamin essential for the synthesis of CoA and ACP (acyl-carrier protein, cofactors in energy yielding reactions including carbohydrate metabolism and fatty acid synthesis. Pantothenate is synthesized de novo by plants and micro-organisms; however, animals obtain the vitamin through their diet. Utilizing our knowledge of the pathway in Escherichia coli, we have discovered and cloned genes encoding the first and last enzymes of the pathway from Arabidopsis, panB1, panB2 and panC. It is unlikely that there is a homologue of the E. coli panD gene, therefore plants must make beta-alanine by an alternative route. Possible candidates for the remaining gene, panE, are being investigated. GFP (green fluorescent protein) fusions of the three identified plant enzymes have been generated and the subcellular localization of the enzymes studied. Work is now being performed to elucidate expression patterns of the transcripts and characterize the proteins encoded by these genes.
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