The medicinal leech: historical perspectives.
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Retzius cells of Hirudo medicinalis were cultivated on extracellular matrix protein so that extended arborizations were formed. The propagation of voltage transients along 1-microns-thick neurites was observed at a resolution of 8 microns at 10 kHz by use of a voltage-sensitive dye. Delay and width of the fluorescence transients caused by hyperpolarization of the soma are described by passive spread of voltage in a homogeneous cable (time constant, 10 ms; space constant, 320 microns). The local sensitivity of the dye was determined from a comparison of the amplitudes of fluorescence and of fitted voltage. The fluorescence transients caused by depolarization were scaled using the sensitivity profile. Action potentials were found to pervade the neurites without significant change of amplitude but with enhanced pulse width.
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In 1826, Jean-Pierre Gama, a French military surgeon, treated a young locksmith who had self-inflicted posttraumatic transient cortical blindness. This may be the earliest detailed, firsthand description of this condition by a medically and scientifically trained observer. Gama's report sheds light on the concept of the mechanism of coup-contrecoup of cerebral concussion and its treatment in the early 19th century and on the germinating discipline of cerebral localization.
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