Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Barium Compounds”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,585 records · Page 88Linked to original sources
PubMed1997

Volume regulation in NIH/3T3 cells not expressing P-glycoprotein. I. Regulatory volume decrease.

Exposure of NIH/3T3 fibroblasts not expressing P-glycoprotein to 50, 30, 20, and 10% hyposmotic solutions led to cell volume increases of 70, 32, 21, and 12%, respectively. After swelling, NIH/3T3 cells exhibited regulatory volume decrease (RVD), attaining complete volume recovery after 30 min except in 50% hyposmotic solution, in which volume recovery was 76%. RVD was accelerated by gramicidin and inhibited by the Cl channel blockers 5-nitro-2-(3-phenylpropylamino)-benzoic acid, 1,9-dideoxyforskolin, dipyridamole, and niflumic acid and by the K channel, blocker quinidine. RVD was reduced 15% by removal of extracellular Ca. The pathway opened by hypotonicity was highly permeable to K and Rb and only partly permeable to other cations. Most anions were able to permeate, with a permeability ranking of nitrate > benzoate = iodide > thiocyanate > chloride > > gluconate. The pathway was permeable to neutral amino acids, with a permeability ranking of glycine > alanine > glutamate > taurine > gamma-aminobutyric acid > glutamine. The pathway was not permeable to basic amino acids. These results show that, despite the absence of P-glycoprotein, NIH/3T3 cells exhibit RVD with properties similar to those expressed in most cell types.

3T3 Cells↗
PubMed1990

Regulation of small-conductance K+ channel in apical membrane of rat cortical collecting tubule.

We used the patch-clamp technique to study the activity and regulation of single potassium channels in the apical membrane of isolated cortical collecting tubules (CCT) of rat kidney. With 140 mM K+ in the pipette the inward conductance of the channel in cell-attached patches at 37 degrees C was 35 pS (n = 106, NaCl-Ringer or 70 mM KCl and 70 mM NaCl in the bath), and the outward conductance was 15 pS (n = 15, 70 mM NaCl + 70 mM KCl in the bath). Mean open probability (Po) of the channel is voltage independent and 0.96 (n = 106). The channel displayed one open state with a mean lifetime of 18.6 ms and one closed state with a mean lifetime of 0.7 ms (n = 20). Selectivity ratio between K+ and Na+ is 20 (n = 5). High-potassium diet increased channel incidence from control 32% (53 patches with channel from 165 patches) to 64% (53 patches with channels from 83 patches). The channel could be blocked by 1 mM Ba2+ (n = 7, Ba2+ in the pipette); however, 5 mM tetraethylammonium (n = 9, TEA in the pipette) did not block the channel activity. The channel was very sensitive to intracellular pH (n = 6). Changing bath pH facing cytoplasmic side of inside-out patches from 7.4 to 6.9 reversibly reduced Po from 0.9 to 0.1. Addition of 1 mM ATP (n = 7) to bath almost completely inhibited channel activity in inside-out patches. This ATP-induced inhibition was fully reversible and was found to be dependent on the ratio of ATP to ADP, since adding 0.5 mM ADP to bath solution relieved the ATP-induced blockade. Results indicate that intracellular pH, concentration of ATP, and ratio of ATP to ADP are important regulators of potassium channel activity in the apical membrane of rat CCT, and the properties of the channel make it a strong candidate for K+ secretion in this nephron segment.

Adenosine Triphosphate↗
PubMed2004

99mTc-stannous colloid white cell scintigraphy in childhood inflammatory bowel disease.

UNLABELLED: 99mTc-Labeled white cell scintigraphy (WCS) has been used for the investigation of inflammatory bowel disease (IBD) in adults, but data on children are limited. The most common agent used is (99m) Tc-hexamethylpropyleneamine oxime (HMPAO); however, this agent has limitations. In a retrospective study, we assessed the use of (99m)Tc-stannous colloid WCS for the initial evaluation of children with suspected IBD. METHODS: Diagnostic, endoscopic, and contrast radiography results were retrospectively collected from the medical records. Two experienced nuclear physicians unaware of the patient data interpreted the WCS results, with agreement reached by consensus. Statistical analysis was performed on the ability of WCS to detect active disease and localize it topographically and on a comparison of diagnostic methods, using a combination of clinical features and endoscopy as the reference standard. RESULTS: Between 1996 and 1999, 64 patients (35 male and 29 female; mean age, 12.5 y; age range, 2-19 y) had WCS performed, with IBD subsequently diagnosed in 34 patients. (99m)Tc-Stannous colloid WCS had an 88% sensitivity, 90% specificity, and 8.8 likelihood ratio for initial investigation of IBD. Agreement was poor for topographic localization of disease. Small-bowel series had a 75% sensitivity, 50% specificity, and 1.5 likelihood ratio for detecting endoscopic disease of the terminal ileum and proximal colon. CONCLUSION: Our results confirm that WCS is a useful imaging technique for the initial evaluation of patients with suspected IBD. (99m)Tc-Stannous colloid had results at least comparable to those of other WCS agents, and in children, (99m)Tc-stannous colloid WCS should be preferred in view of lower cost, shorter preparation time, and the smaller blood volumes required.

Adult↗
PubMed1995

[Clinical response of different colonic segments to chemical denervation: an experimental study].

An experimental study in rats with chemical myenteric plexus destruction of different segments of large intestine is presented. Forty Wistar rats weighing 200-250 g were used. Four groups of ten animals each were made. Group I: control; group II: myenteric denervation of 4 cm segment of rectosigmoid; group III: myenteric denervation of 4 cm segment of descending colon; group IV: myenteric denervation of 4 cm segment of transverse colon. Myenteric denervation was produced by serosal application of 0.1% benzalconium chloride during 30 minutes. Animals were evaluated by clinical parameters (survival, weight change, food intake, nutritional state), radiological (barium enema) and histomorphometry. Group IV showed the greatest mortality, nutritional deterioration and colonic obstruction while the results of group II are similar to control group.

Animals↗
PubMed2003

Diethylstilbestrol and tetrahydrochrysenes are calcium channel blockers in human platelets: relationship to the stilbene pharmacophore.

The effects of compounds with the stilbene pharmacophore [diethylstilbestrol (DES), DES derivatives, tetrahydrochrysene (THC), and THC derivatives] were examined for their ability to inhibit thrombin-induced Ca(2+) influx in human platelets. DES derivatives (DES dimethyl ether, DES dipropionate, dienestrol, and hexestrol) had lower inhibitory activity than DES. Esterification of DES with the bulky monobenzyl group eliminated inhibitory activity. Unsubstituted THC diol had the lowest inhibitory activity in the series of the THC derivatives bearing substituents in the 5,11 positions. These derivatives, either diethyl or dipropyl, cis or trans, were potent inhibitors of thrombin-induced [Ca(2+)](i) elevation (near 100% inhibition at 10 microM). Therefore, stilbene pharmacophore having bulk out of the plane of the double bond (from the twisting of the two aromatic rings or from addition of all substituents) seems to be requirement for the inhibitory activity. Free hydroxyl groups are also required for inhibitory activity, most likely for hydrogen bonding, since trans-diethyl tetrahydrochrysene dimethyl ether was inactive. Compounds bearing ethyl substituents (DES and THC derivatives) inhibited thrombin-induced release of calcium from the endoplasmic reticulum. These compounds also inhibited thapsigargin-induced Ca(2+) influx. This result implies that these compounds also block store-operated Ca(2+) influx directly, as well as internal Ca(2+) release. Compounds without ethyl substituents (trans-resveratrol, genistein, daidzein, and THC diol) only inhibited calcium influx into platelets.

Adult↗
PubMedDate not supplied

An example of abnormal glow curves identification in personnel thermoluminescent dosimetry.

The personal Dosimetry Service Seibersdorf analyses monthly a large number of thermoluminescent dosimeters (TLD). The dosimeters consist of LiF chips, and the readout is carried out with an automated Harshaw 8800 reader system. In some cases, the luminescent glow curves of the routine analysis do not have the expected form as a result of external chemical contamination, hardware problems, poor heat transfer, etc. It is therefore necessary to investigate the reasons for the irregularity of these curves. An algorithm for the investigation of the routine curves was developed. It is based on the fact that the shape of an abnormal glow curve differs from the shape of a normal one. An interesting type of abnormal glow curves in the routine service was found. Some dosimeters of a certain client, a steel industry, exhibit glow curves with an atypical shape and very high signals. In those dosimeters, a possible chemical contamination in the form of a powder was discovered, which interferes with the dosimetric signal. A quantitative analysis of that powder was made by means of inductively coupled plasma emission spectroscopy (ICP-OES) after microwave dissolution. Elements like aluminium, barium, calcium and others were found. Such elements are used in different combinations as thermoluminescent materials.

Algorithms↗
PubMed2004

pH-dependent isolations and spectroscopic, structural, and thermal studies of titanium citrate complexes.

Titanium(IV) citrate complexes (NH(4))(2)[Ti(H(2)cit)(3)].3H(2)O (1), (NH(4))(5)[Fe(H(2)O)(6)][Ti(H(2)cit)(3)(Hcit)(3)Ti].3H(2)O (2), Ba(2)[Ti(H(2)cit)(Hcit)(2)].8H(2)O (3), and Ba(3)(NH(4))(7)[Ti(cit)(3)H(3)(cit)(3)Ti].15H(2)O (4) (H(4)cit = citric acid) were isolated in pure form from the solutions of titanium(IV) citrate with various countercations. The isolated complexes were characterized by elemental analyses, IR spectra, and (1)H NMR and (13)C NMR spectra. The formation of titanium(IV) citrate complexes depends mainly on the pH of the solutions, that is, pH 1.0-2.8 for the formation of ammonium titanium(IV) citrate 1, pH 2.5-3.5 for ammonium iron titanium(IV) citrate 2, pH 2.8-4.0 for dibarium titanium(IV) citrate 3, and pH 5.0-6.0 for ammonium barium titanium(IV) citrate 4. X-ray structural analyses revealed that complexes 2-4 featured three different protonated forms of bidentate citrate anions that chelate to the titanium(IV) atom through their negatively charged alpha-alkoxyl and alpha-carboxyl oxygen atoms. This is consistent with the large downfield shifts of the (13)C NMR spectra for the carbon atoms bearing the alpha-alkoxyl and alpha-carboxyl groups. The typical coordination modes of the barium atoms in complexes 3 and 4 are six-coordinated, with three alpha-alkoxyl groups and three beta-carboxyl groups of citrate ions. The strong hydrogen bonding between the beta-carboxylic acid and the beta-carboxyl groups [2.634(8) A for complex 2, 2.464(7) A for complex 3, and 2.467(7) A for complex 4] may be the key factor for the stabilization of the citrate complexes. The decomposition of complex 3 results in the formation of a pure dibarium titanate phase and 4 for the mixed phases of dibarium titanate and barium titanate at 1000 degrees C.

Citric Acid↗
PubMed2005

Surface complexation of DNA with insoluble monolayers. Influence of divalent counterions.

DNA interacts with insoluble monolayers made of cationic amphiphiles as well as with monolayers of zwitterionic lipids in the presence of divalent ions. Binding to dioctadecyldimethylammonium bromide (DODAB) or distearoyl-sn-glycero-3-phosphocholine (DSPC) monolayers in the presence of calcium is accompanied by monolayer expansion. For the positively charged DODAB monolayer, this causes a decrease of surface potential, while an increase is observed for the DSPC monolayers. Binding to dipalmitoyl-sn-glycero-3-phosphocholine preserves most of the liquid expanded-liquid condensed coexistence region. The liquid condensed domains adopt an elongated morphology in the presence of DNA, especially in the presence of calcium. The interaction of DNA with phospholipid monolayers is ion specific: the presence of calcium leads to a stronger interaction than magnesium and barium. These results were confirmed by bulk complexation studies.

Animals↗
PubMed1993

Voltage-activated ionic currents in goldfish pituitary cells.

The release of gonadotropin and growth hormone from goldfish pituitary cells has been shown to be dependent on the entry of extracellular Ca2+ through voltage-sensitive Ca2+ channels by pharmacological studies. As a first step to further investigate the involvement of voltage-dependent ion channels in the regulation of anterior pituitary hormone release in the goldfish, cell excitability and voltage-dependent ion currents were characterized using tight-seal whole-cell recordings in dispersed goldfish pituitary cells. Cultured goldfish pituitary cells had an average membrane potential of -36 +/- 3 mV. When held at membrane potentials more negative than -60 mV, these cells were excitable, responding to depolarizing current pulses or anode break with the firing of single action potentials. Results from total current voltage-clamp recordings suggested that all goldfish pituitary cells possess voltage-dependent Na+, Ca2+, and K+ currents. These currents were further characterized independently under isolated current recording conditions. The rapid, transient Na+ current activated at voltages more positive than -40 mV and was sensitive to tetrodotoxin. The steady state inactivation of this Na+ current was also voltage-dependent; at the measured resting potential, > or = 50% of the Na+ current was not available for activation. The voltage-dependence and activation kinetics of the tetraethylammonium-sensitive K+ current resembled those of the delayed rectifier K+ current. The K+ current activated slowly at potentials more positive than -40 mV, and showed little inactivation over the duration of a 1-sec depolarizing pulse. Steady-state inactivation characteristics indicated that < 50% of the K+ current was inactivated at resting potentials. Experiments with 4-aminopyridine indicated the presence of an early transient K+ current that activated in a similar voltage range as the delayed rectifier current. Using barium as the charge carrier to measure Ca2+ currents, a high-voltage activated, long-lasting Ca2+ current was revealed. This "L-type" Ca2+ current activated at potentials more positive than -30 mV and was inhibited by verapamil and nifedipine. This study indicates that goldfish pituitary cells possess the electrophysiological properties required for the participation of voltage-sensitive ion channels in the regulation of hormone release.

4-Aminopyridine↗
PubMed1998

Intracellular acidification induced by passive and active transport of ammonium ions in astrocytes.

We describe an unconventional response of intracellular pH to NH4Cl in mouse cerebral astrocytes. Rapid alkalinization reversed abruptly to be replaced by an intense sustained acidification in the continued presence of NH4Cl. We hypothesize that high-velocity NH4+ influx persisted after the distribution of ammonia attained steady state. From the initial rate of acidification elicited by 1 mM NH4Cl in bicarbonate-buffered solution, we estimate that NH4+ entered at a velocity of at least 31.5 nmol.min-1.mg protein-1. This rate increased with NH4Cl concentration, not saturating at up to 20 mM NH4Cl. Acidification was attenuated by raising or lowering extracellular K+ concentration. Ba2+ (50 microM) inhibited the acidification rate by 80.6%, suggesting inwardly rectifying K+ channels as the primary NH4+ entry pathway. Acidification was 10-fold slower in rat hippocampal astrocytes, consistent with the difference reported for K+ flux in vitro. The combination of Ba2+ and bumetanide prevented net acidification by 1 mM NH4Cl, identifying the Na(+)-K(+)-2Cl- cotransporter as a second NH4+ entry route. NH4+ entry via K+ transport pathways could impact "buffering" of ammonia by astrocytes and could initiate the elevation of extracellular K+ concentration and astrocyte swelling observed in acute hyperammonemia.

Acids↗
PubMed1997

Determination of NH4+/NH3 fluxes across apical membrane of macula densa cells: a quantitative analysis.

Luminal addition of 20 mM NH4+ produced a rapid acidification of rabbit macula densa (MD) cells from 7.50 +/- 0.06 to 6.91 +/- 0.05 at an initial rate of 0.071 +/- 0.008 pH unit/s. In the luminal presence of 5 microM bumetanide, 5 mM Ba2+ or both, the acidification rate was reduced by 57%, 35% and 93% of control levels. In contrast, intracellular pH (pHi) recovery after removing luminal NH4+ was unaffected by bumetanide and Ba2+ but was sensitive to 1 mM luminal amiloride (71% inhibition). The bumetanide-sensitive acidification rate represents most certainly the NH4+ flux mediated by the apical Na+:K+ (NH4+):2Cl- cotransporter, but the Ba(2+)-sensitive portion does not seem to be associated with the apical K+ channels previously characterized by us. The effects of NH4+ entry across the apical membrane were simulated using a simple model involving five adjustable parameters: apical and basolateral permeabilities for NH4+ and NH3 and a parameter describing a pH-regulating mechanism. The model shows that the apical membrane of MD cells is much more permeable to NH3 than it is to NH4+ and, under control conditions, the apical NH4+ flux appears surprisingly high (11-20 mM/s) and challenges the notion that MD cells present a low intensity of ionic transport.

Amiloride↗
PubMed2004

Modulation of single channels underlying hippocampal L-type current enhancement by agonists depends on the permeant ion.

The influx of calcium (Ca(2+)) ions through L-type channels underlies many cellular processes, ranging from initiation of gene transcription to activation of Ca(2+)-activated potassium channels. L-type channels possess a diagnostic pharmacology, being enhanced by the dihydropyridine BAY K 8644 and benzoylpyrrole FPL 64176. It is assumed that the action of these compounds is independent of the ion conducted through the channel. In contrast to this assumption, modulation of L-type channel activity in acutely dissociated rat CA1 hippocampal neurons depended on the divalent ion identity. BAY K 8644 and FPL 64176 substantially increased single-channel open time only when barium (Ba(2+)) was the permeant ion. BAY K 8644 increased single-channel conductance when either Ba(2+) or Ca(2+) ions were the charge carrier, an effect not observed with FPL 64176. BAY K 8644 enhanced the whole cell L-type channel Ca(2+)- or Ba(2+)-carried current without a change in deactivation tail kinetics. In contrast, enhancement by FPL 64176 was associated with a dramatic slowing of deactivation kinetics only when Ba(2+) and not Ca(2+) was the charge carrier. Current activation was slowed by FPL 64176 with either charge carrier, an effect arising from a clustering of agonist-modified long-duration openings toward the end of the voltage step. These data indicate that agonists enhanced L-type current by distinct mechanisms dependent on the permeant ion, indicating that care must be considered when used as diagnostic tools.

3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethy↗