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PubMed1975

Outbursting wedges of the colonies of Clostridium welchii containing chain forming mutants after ultra violet irradiation.

Cl. welchii NCTC 6785 was irradiated with UV light. The 40 minutes irradiated cells after 3-8 days ageing at room temperature (22-25 degrees C) and 48 hours initial incubation at 37 degrees C produced hairy outbursting wedges. These wedges gave rise to the colonies composed of chain forming mutants, which on transfer into the fluid medium grew into a pure chain forming culture.

Cell Division↗
PubMed1991

Is interindividual variation of cellular radiosensitivity real or artifactual?

A recently developed dose-survival assay using human G0 T lymphocytes from peripheral blood was employed to assess possible interindividual variation of cellular radiosensitivity by comparing variability between a single test for different individuals and repeated tests for a single donor. The surviving fraction at each X-ray dose level fluctuated similarly between the two groups, and the X-ray dose required to kill 90% of the cells (D10) was 3.59 +/- 0.18 Gy (mean +/- SD) for 31 different individuals and 3.66 +/- 0.21 Gy for 28 repeated tests of one individual. Analysis of variance to compare the two sets of data showed that variation in the D10 value was not significantly greater in the former group. Analysis of D50 and D90 showed similar results. These results support the hypothesis that interindividual variation in cellular radiosensitivity is quite small, if it exists at all, as far as can be determined by the loss of colony-forming ability of irradiated G0 lymphocytes.

Cell Survival↗