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PubMed2002

Mechanical properties of skeletal bone in gene-mutated stöpsel(dtl28d) and wild-type zebrafish (Danio rerio) measured by atomic force microscopy-based nanoindentation.

An atomic force microscopy (AFM)-based nanoindenter was used to evaluate the mechanical properties of skeletal bones in wild-type and gene-mutated zebrafish (Danio rerio), stöpsel(dtl28d). Both skeletons were isolated from adult zebrafish and tested under a load of 5 mN. It was found that stp/stp bone has a similar nanohardness but significantly greater elastic modulus compared with that of wild-type bone. The residual indenter impressions using AFM and the fracture surfaces of both bones using scanning electron microscopy were examined and showed that the bone of zebrafish becomes more brittle after the stp mutation. This first observation of the alteration of bone mechanical behavior by gene mutation in zebrafish system is of scientific and clinical relevance to many areas of study, such as bone fracture and fragility mechanisms in human heritable disorders and bone-materials fabrication via gene engineering.

Animals↗
PubMed1996

Human stratum corneum lipids have a distorted orthorhombic packing at the surface of cohesive failure.

The cohesive strength of the stratum corneum is determined by its unique molecular composition and structural architecture. Whereas the structure responsible for corneocyte cohesion has been visualized at the microscopic level, the structure of the intercellular domain has not been characterized at the molecular level. In this report, new insight into the molecular architecture of the stratum corneum has been provided by atomic force microscopy and x-ray photoelectron spectroscopy. The surface layer of human stratum corneum was stripped, yielding the characteristic polygonal corneocytes shown by scanning electron microscopy as well as low resolution atomic force microscopy. With atomic force microscopy, the resolution was increased to allow imaging of the molecular architecture of the stratum corneum. With the high resolution image, a repetitive pattern characteristic of lipids in an ordered state was visualized. The lattice appeared to be orthorhombic where the lattice distances were about 5.5 and 9 angstroms, and the lattice angle was close to 90 degrees. The atomic composition of the superficial layers was 82% carbon, 16.5% oxygen, and 1.4% nitrogen as determined by x-ray photoelectron spectroscopy. The high nitrogen content compared to the calculated stratum corneum lipid composition and measured model lipid composition suggests that proteins were detected. In summary, although proteins are present, the fracture plane of the stratum corneum is largely composed of lipids that appear to have a distorted orthorhombic packing.

Female↗
PubMed2006

Fabrication of polymeric replicas of cell surfaces with nanoscale resolution.

We report an approach for fabricating biomimetic surface replicas of cells with nanoscale resolution. Fixed cells serve as a template for a two-stage replica molding process. Cast from the template, the impression replica contains a reproduction of cellular topographical features indented into its surface, and cast from the impression replica, the relief replica contains a copy of these features protruding from its surface. Various polymers and cells can be utilized, and scanning electron microscopy, atomic force microscopy, and white light interferometry analyses confirm the replication of nanoscale features. These replicas are useful for investigating cellular function and for biomimetic tissue engineering.

Animals↗