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PubMed1999

Efferent connections of the anteromedial nucleus of the thalamus of the rat.

The projections from the anteromedial nucleus of the thalamus (AM) were investigated using anterograde and retrograde tracing techniques. AM projects to nearly the entire rostrocaudal extent of limbic cortex and to visual cortex. Anteriorly, AM projects to medial orbital, frontal polar, precentral agranular, and infraradiata cortices. Posteriorly, AM projects to retrosplenial granular, entorhinal, perirhinal and presubicular cortices, and to the subiculum. Further, AM projects to visual cortical area 18b, and to the lateral and basolateral nuclei of the amygdala. AM projections are topographically organized, i.e., projections to different cortical areas arise from distinct parts of AM. The neurons projecting to rostral infraradiata cortex (IRalpha) are more caudally located in AM than the neurons projecting to caudal infraradiata cortex (IRbeta). The neuronal cell bodies that project to the terminal field in area 18b are located primarily in ventral and lateral parts of AM, whereas neurons projecting to perirhinal cortex and amygdala are more medially located in AM. Injections into the most caudal, medial part of AM (i.e., the interanteromedial [IAM] nucleus) label terminals in the rostral precentral agranular, caudal IRbeta, and caudal perirhinal cortices. Whereas most AM axons terminate in layers I and V-VI, exceptions to this pattern include area 18b (axons and terminals in layers I and IV-V), the retrosplenial granular cortex (axons and terminals in layers I and V), and the presubicular, perirhinal, and entorhinal cortices (axons and terminals predominantly in layer V). Together, these findings suggest that AM influences a widespread area of limbic cortex.

Animals↗
PubMed1999

Automatic detection of bursts in spike trains recorded from the thalamus of a monkey performing wrist movements.

In a previous paper (Churchward PR, Butler EG, Finkelstein DI, Aumann TD, Sudbury A, Horne MK. J Neurosci Methods 1997;76:203-210), we showed that a simple back propagation neural network could reliably model visual inspection by human observers in detecting the point of change of neuronal discharge patterns. The data for that study was deliberately chosen so that the point of change was readily detected and there would be high concordance between human observers. We wished to extend this investigation by comparing a variety of automatic analysis methods on more complex data sets. Two automatic analysis methods have been discussed in this paper. The knowledge based spike train analysis (KBSTA) was designed to emulate the detection of bursts by human observers. The self-organizing feature map (SOFM) spike train analysis determined a burst by classifying the patterns of neuronal discharge. Neuronal discharge was recorded from the motor thalamus and nucleus ventralis posterior lateralis caudalis (VPLc) of a monkey performing consecutive trials of skilled wrist movements. Recordings were made from 36 neurons whose discharge patterns were related to wrist movement. Three hundred and sixty trials performed during the recording of these 36 neurons were chosen at random and used to compare the three methods, KBSTA, SOFM, and visual inspection. The main results of this study show that for the 360 trials the three detection methods have very similar results in detecting the onset and offset of neuronal bursts. The SOFM method is not the best first approach for detecting a burst, but it does provides independent evidence to support the KBSTA and visual inspection methods. In conclusion we propose the KBSTA method as a practical, automatic technique to identify bursts of neuronal discharge.

Action Potentials↗
PubMed2002

Paradoxical locomotor activating effects of kappa-opioid receptor stimulation in the preweanling rat: role of the ventromedial thalamus and superior colliculus.

Stimulating kappa-opioid receptors in the substantia nigra pars reticulata robustly increases the locomotor activity of preweanling rats. To determine whether nigrothalamic and nigrotectal connections are necessary for this kappa-opioid-mediated locomotor activity, preweanling rats were given a systemic injection of saline or 5 mg/kg U50,488 (a kappa-opioid receptor agonist) 2 days after receiving sham or bilateral electrolytic lesions of the ventromedial thalamus (VMT) or superior colliculus (SC). Results showed that lesions of the VMT and SC attenuated the U50,488-induced locomotor activity of preweanling rats, indicating that the locomotor activating effects of kappa-opioid receptor stimulation require that nigrothalamic and nigrotectal connections be intact.

3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cycloh↗
PubMed2002

Role of the anterodorsal and anteroventral nuclei of the thalamus in spatial memory in the rat.

This study tests the hypothesis that the anterior thalamic nuclei play a significant role in spatial learning and memory. Adult, male Sprague-Dawley rats with bilateral ibotenic acid lesions of the anterior thalamus were tested for 5 days in a repeated acquisition water maze task. Compared with Controls, rats with nearly complete lesions of both anterodorsal (AD) and anteroventral (AV) thalamic nuclei (AD/AV) were only mildly impaired in their spatial learning and memory. Larger lesions that extended into the anteromedial (AM) thalamic nucleus (AD/AV+) caused a more severe impairment and complete lesions of all three anterior nuclei (AD/AV/AM) resulted in even greater impairment that extended to all aspects of the task. In probe trials, only the Control animals had a preference for the correct quadrant. Approximately one-half of the rats were tested for a second week to determine if the impaired groups would benefit from further training. AD/AV/AM rats showed little improvement, but the other groups all improved significantly in all aspects of the task except the probe trial. Together, these data indicate that the anterior thalamic nuclei contribute to spatial learning and memory, but neither AV nor AD independently plays a dominant role.

Animals↗
PubMed1999

The involvement of the mediodorsal nucleus of the thalamus and the midbrain extrapyramidal area in locomotion elicited from the ventral pallidum.

Motor activity is regulated by projections from the nucleus accumbens to the ventral pallidum, but it is unclear which efferents regulate behavioral output from the ventral pallidum. Motor activity was elicited pharmacologically by microinjecting either the mu opioid receptor agonist, Tyr-D-Ala-Gly-NmePhe-Gly-OH (DAMGO) or the glutamate receptor agonist, alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionate (AMPA) into the ventral pallidum. The involvement of efferent projections was determined by microinjecting the local anesthetic procaine into the mediodorsal nucleus of the thalamus (MD) or the midbrain extrapyramidal area (MEA) prior to administering DAMGO or AMPA into the ventral pallidum. The motor activity induced by DAMGO was blocked by procaine microinjected into either the MD or the MEA. In contrast, procaine microinjected into the MD did not block motor activity elicited by AMPA while procaine into the MEA abolished the behavioral activation. These data indicate that the involvement of efferent projections from the ventral pallidum to either the MD or MEA in motor activation depends upon the type of receptor stimulated in the ventral pallidum.

Analgesics, Opioid↗
PubMed2000

Mechanical and thermal hypersensitivity develops following kainate lesion of the ventral posterior lateral thalamus in rats.

Damage to the ventral-posterior lateral nucleus (VPL) of the thalamus or its afferent pathways can produce moderate to severe on-going pain and pain in response to normally innocuous stimuli (allodynia) and hypersensitivity to mildly noxious stimuli (hyperalgesia). The present study measured the responses to mechanical and thermal stimuli before and 2, 8, 24 and 48 h after a kainate-induced lesion of the VPL in male rats. Compared with control animals, hypersensitivity to mechanical stimulation of the hindpaw was apparent by 24 h post-lesion. At 48 h, the frequency of mechanical response increased from a baseline response frequency of 17+/-4.7 to 46+/-11.6%. Thermal withdrawal latencies 48 h after the lesion decreased from a baseline latency of 9.9+/-1.8 to 5.3+/-0.4 s. It is concluded that a neurotoxic lesion of the VPL results in a delayed onset of mechanical and thermal hyperalgesia. This study suggests a potential model for studying the basic mechanisms and potential therapies for central pain syndrome.

Afferent Pathways↗
PubMed2002

Reversible changes of presumable synaptic connections between primary somatosensory cortex and ventral posterior lateral thalamus of rats during temporary deafferentation.

Many single neurons were simultaneously recorded from forepaw areas of both primary somatosensory cortex and the ventral posterior lateral thalamus of anesthetized rats to characterize the changes of presumable excitatory synaptic connections between two nuclei following temporary deafferentation (TD). Thalamic and cortical interactions were examined by analyzing spike-triggered cross-correlation histograms (STCCHs, n=426). Before TD, 46.48% of STCCHs exhibited thalamocortical (TC) excitation and 7.51% of STCCHs showed corticothalamic (CT) connectivity. After TD, these connections were less frequently observed (after 20 min of TD, TC: 13.38% of STCCHs, CT: 5.40% of STCCHs). Fifty-seven TC and nine CT connections were reversibly suppressed during TD. However, 23 CT connections were reversibly augmented following TD. These results imply that temporary blocking of afferent information may induce system-wide plasticity involving corticofugal modulation.

Afferent Pathways↗
PubMed1998

Removal of GABAergic inhibition in the mediodorsal nucleus of the rat thalamus leads to increases in heart rate and blood pressure.

The mediodorsal nucleus of the thalamus (MD) has connections with central autonomic centers involved in cardiovascular control and undergoes severe degeneration in fatal familial insomnia, a human disease characterized by progressive dysautonomia. Microinjections of the GABAA antagonist bicuculline methiodide (BMI) into the medial and central portion of the MD lead to significant, dose-dependent increases in both heart rate and blood pressure. Similar injections into surrounding regions elicited little to no change. The data suggest that the medial and central portion of the MD plays a role in central cardiovascular regulation. Neurons of the MD may be under tonic GABAergic inhibition, and disruption of circuits at this level may underlie dysautonomia in many neurological diseases.

Animals↗
PubMed2001

Projections from the marginal zone and deep dorsal horn to the ventrobasal nuclei of the primate thalamus.

It has been concluded recently that if a projection from the marginal zone to the ventral posterior lateral (VPL) nucleus exists, it is sparse. Given the importance of the marginal zone in nociception, this conclusion has raised doubts about the significance of the role of the ventrobasal complex in nociception. We have reexamined this projection using injections of the retrograde tracer, cholera toxin subunit B, into one side of the lateral thalamus in macaque monkeys. The injections were confined to the ventrobasal complex (with minimal spread to adjacent nuclei that do not receive spinal projections) in two animals. Many retrogradely labeled neurons were found in lamina I (as well as in lamina V) of the contralateral spinal and medullary dorsal horn. The results are consistent with the view that neurons in the marginal zone contribute prominently to the spinothalamic and trigeminothalamic projections to the VPL and ventral posterior medial (VPM) nuclei. This pathway is likely to be important for the sensory-discriminative processing of nociceptive information with respect to the location and intensity of painful stimuli.

Animals↗
PubMed2000

Effect of high-frequency stimulation of the subthalamic nucleus on the neuronal activities of the substantia nigra pars reticulata and ventrolateral nucleus of the thalamus in the rat.

Electrophysiological recordings were made in anaesthetized rats to investigate the mode of function of high-frequency stimulation of the subthalamic nucleus used as a therapeutic approach for Parkinson's disease. High-frequency electrical stimulation of the subthalamic nucleus (130 Hz) induced a net decrease in activity of all cells recorded around the site of stimulation in the subthalamic nucleus. It also caused an inhibition of the majority of neurons recorded in the substantia nigra pars reticulata in normal rats (94%) and in rats with 6-hydroxydopamine lesions of the substantia nigra pars compacta (90%) or with ibotenic acid lesions of the globus pallidus (79.5%). The majority of cells recorded in the ventrolateral nucleus of the thalamus responded with an increase in their activity (84%). These results show that high-frequency stimulation of the subthalamic nucleus induces a reduction of the excitatory glutamatergic output from the subthalamic nucleus which results in deactivation of substantia nigra pars reticulata neurons. The reduction in tonic inhibitory drive of nigral neurons induces a disinhibition of activity in the ventrolateral motor thalamic nucleus, which should result in activation of the motor cortical system.

Adrenergic Agents↗
PubMed2003

Neuronal interactions are higher in the cortex than thalamus in the somatosensory pathway.

Previous studies have shown significant correlated discharges (noise correlation) and synergistic information coding among adjacent cortical neurons. In order to investigate whether such interactions are present at an earlier stage of sensory processing, we compared noise correlation and synergistic information transmission in the ventral posterolateral nucleus (VPLn) of thalamus and primary somatosensory cortex (SI) of anesthetized rats. A hind paw was stimulated electrically and responses of several neighboring neurons were recorded simultaneously with a tetrode. Analyses indicated that noise correlation in the SI was about four times higher than in the VPLn, and, interestingly, it was significantly reduced following sensory stimulation in both regions. Spike count distributions of individual VPLn units contained higher amounts of information about the delivery of external stimulation compared with those of SI units. When simultaneously recorded units were considered together, transmission of information was more interactive (synergistic or redundant) among SI than VPLn units. On average, information transmission was independent in the VPLn, but synergistic in the SI. The difference in synergistic information coding was largely attributable to different levels of noise correlation and their modulation by external sensory stimulation. These results indicate that neuronal interactions are relatively low at the thalamic level, but much enhanced at the cortical level along the somatosensory pathway. The enhanced neuronal interactions in the cortex may reflect the role of cortex in extracting higher features of sensory stimuli.

Action Potentials↗
PubMed1997

Postnatal development of GABA-immunoreactive terminals in the reticular and ventrobasal nuclei of the rat thalamus: a light and electron microscopic study.

The postnatal development of inhibitory GABAergic circuits in the thalamic reticular and ventrobasal nuclei was studied in rats ranging from the day of birth to the end of the third postnatal week by means of a postembedding immunogold staining procedure to visualize GABA. In the reticular nucleus, GABA labeling was present from birth in cell bodies, dendrites, growth cones and a few synaptic terminals, whereas in the ventrobasal nucleus it was exclusively in axonal processes identifiable as growth cones, vesicle-rich profiles and synaptic terminals. In both nuclei, GABA-labeled synaptic terminals were, however, very scarce and immature in neonatal animals and they became numerous and morphologically mature only after the end of the second postnatal week. These findings suggest that inhibitory synaptic responses in the somatosensory thalamus are not yet fully mature throughout the first two postnatal weeks and support the hypothesis that GABA may initially play trophic roles. The relatively late maturation of the thalamic GABAergic system may have important functional consequences, as the reticulothalamic circuits are responsible for the generation of spindle wave oscillations whose cellular mechanisms are also involved in the generation of spike-and-wave (absence) seizures in humans and in animal models.

Animals↗
PubMed2003

Dlx transcription factors regulate differentiation of dopaminergic neurons of the ventral thalamus.

Recent studies have provided many lines of evidence that specific homeodomain factors act to regulate differentiation into specific neuron types. However, these studies have mainly focused on the caudal CNS, while in the forebrain, the regulation of neuron specification remains relatively unknown. To investigate the genetic regulatory networks that control neuron differentiation in the forebrain, we have analyzed the expression patterns and functions of DLX homeodomain factors in the ventral thalamus of early mouse embryos. During initial neurogenesis (E9.5-E10.5), DLX(+) cells are the first progenitors to make terminal divisions and differentiate as neurons. We have defined a set of regulatory genes coexpressed with DLX, in both progenitors (PAX6 and MASH1) and in the differentiating neurons (PAX6, along with a combination of LIM-type homeodomain factors, including ISL1, Lhx1/Lim1, and Lhx5/Lim2). These initial neurons express tyrosine hydroxylase (TH), and become the PAX6-expressing A13 dopaminergic neurons of the zona incerta. To test for DLX function, the initial differentiation of the ventral thalamic neurons was examined in embryos mutant for Dlx1 and Dlx2. Dlx1/2 double homozygous mutants formed ventral thalamic neurons, but these neurons lacked PAX6, ISL1, and TH expression. These data suggest that DLX genes act as forebrain-specific factors linking general neuron-inducing signals to region-specific neuron differentiation programs.

Animals↗
PubMedDate not supplied

Distribution of terminals from pedunculopontine tegmental nucleus and synaptic organization in lateralis medialis-suprageniculate nucleus of cat's thalamus: anterograde tracing, immunohistochemical studies, and quantitative analysis.

The cat's lateralis medialis-suprageniculate nuclear complex (LM-Sg) in the thalamus receives input from various brain regions such as the superior colliculus, brain stem, and spinal cord, as well as from visual association cortex. In a previous study, we demonstrated that LM-Sg receives cholinergic fibers from the pedunculopontine tegmental nucleus (PPT) and that cholinergic terminals make synaptic contacts with the dendrites of glutamatergic projection neurons and of GABAergic interneurons (Hoshino et al., 1997). In this study, we investigate the distribution and the organization of PPT terminals by means of a combined anterograde tracer (biotinylated dextran amine, BDA) and immunohistochemical methods. When stained by acetylcholinesterase (AChE), the LM-Sg is not uniformly immunoreactive, but rather is patchily labeled and shows a streaming type of reactivity. The tissue content appears high in enzyme activity in AChE-positive zones and is much lighter in activity in AChE-negative zones. We compared the synaptic organization between AChE-positive and AChE-negative portions of the LM-Sg in separate groups of electron-microscopic material: four types of vesicle containing profiles (RS, RL, F1, and PSD) as well as synaptic glomeruli were observed in this nucleus. Among these, the PSD profiles were observed more frequently in AChE-positive portions than in AChE-negative zones. Furthermore, the number of glomeruli was significantly higher in AChE-positive than in AChE-negative zones. Following the injection of BDA into PPT, labeled terminals within LM-Sg were rather more concentrated in the AChE-positive portion. Although the majority of PPT terminals made synaptic contacts with dendrites in the neuropil, a few terminals were involved in the synaptic glomeruli. The present results show that the synaptic organization is distinctly different between the AChE-positive and AChE-negative portions of LM-Sg. These results suggest that the AChE-positive portions of LM-Sg are relatively more involved in integrating information arising from a diverse set of inputs and processing that information within glomeruli in a complex manner than occurs in the AChE-negative portion of LM-Sg.

Acetylcholinesterase↗
PubMedDate not supplied

The role of the ventrolateral nucleus of the thalamus in the switching of descending influences to motor activity in the rat.

Studies on rats showed that the facilitating influence of preliminary transection of the rubrospinal tract on recovery of motor activity and operant reflexes disrupted by lesioning of the red nucleus was more apparent when lesioning was chemical than when lesioning was electrolytic. This is due to the survival of cerebellothalamic fibers to the ventrolateral nucleus of the thalamus after chemical lesioning of the red nucleus with quinolinic acid. It was also shown that preliminary lesioning of the ventrolateral thalamic nucleus strongly hindered the switching of motor activity under the control of the corticospinal tract in rats subjected to section of the rubrospinal tract and lesioning of the red nucleus.

Animals↗
PubMed1996

Gustatory thalamus lesions in the rat: I. Innate taste preferences and aversions.

Two experiments examined the innate taste preferences and aversions of rats with electrolytic lesions of the gustatory thalamus (GT). Contrary to previous research, GT lesions had only a minor influence on intake of the 4 basic tastes as assessed with the 24-hr, 2-bottle preference test in Experiment 1. The same lesioned rats, when tested with the same stimuli in the 15-min, single-bottle procedure in Experiment 2, showed normal consumption patterns except for sucrose intake, which was attenuated. The conflicting findings of previous and present research are considered to result from differences in lesion size. The current data suggest that the GT has a relatively minor functional role in the unconditioned acceptance or rejection of sapid stimuli.

Animals↗
PubMed1997

Lesions of the mediodorsal nucleus of the thalamus and classical eyeblink conditioning under less-than-optimal stimulus conditions: role of partial reinforcement and interstimulus interval.

Rabbits received lesions of the mediodorsal nucleus of the thalamus (MD) or sham lesions and were subjected to classical eyeblink (EB) and heart rate (HR) conditioning. Separate groups of sham and lesioned animals received either 50% or 25% reinforcement with a periorbital shock unconditioned stimulus. Other groups received an interstimulus interval of either 1.0 or 1.5 s. Animals with MD lesions acquired the EB conditioned response (CR) more slowly than sham-lesioned animals with either the 1.5-s ISI or with the 25% reinforcement schedule. The lesions had no significant effect on the HR CR, however. Results suggest that information processed by MD is relayed to the prefrontal cortex and is required for somatomotor response selection under nonoptimal learning conditions.

Animals↗
PubMed1999

Progressive ratio performance in rats with gustatory thalamus lesions.

To determine whether damage of the gustatory thalamus (the parvicellular region of the ventroposteromedial nucleus) disrupts the perceived value of reinforcing stimuli, the performance of rats with bilateral, electrophysiologically guided, electrolytic lesions of the area was examined in Experiment 1 with a progressive-ratio schedule of reinforcement. Lesioned rats showed normal concentration-dependent changes in break point (an index of the amount of effort a subject will expend to obtain reinforcement) and in consummatory responding (licking) for the sucrose reward. In the reward comparison procedure of Experiment 2, however, the same lesioned rats failed to show morphine-induced suppression of alanine consumption. The results provide no support for the view that the thalamic taste area is involved in the perception of the absolute reinforcing value of gustatory stimuli.

Alanine↗