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Frozen preservation of human amnion and its use as a burn wound dressing.

A method of freezing and sterilizing the human amnion enabled it to be stored at -60 degrees C for more than six months. The membranes were obtained from twenty-four cesarean section mothers without a history of endometritis, premature rupture of their membranes or a positive serology. The elongation and break strength of the membranes were evaluated before and after storage. The membranes as prepared have been utilized on twenty-two wounds of ten burn patients. The results indicate that amnion is quite safe and effective as a biological dressing using this preservation method.

Adult↗

Modern dressings: what to use.

There have been significant advances in the understanding of wounds and wound management since the 1960s. The author briefly discusses these advances and provides a practical introduction to current dressing routines.

Alginates↗

[Vacuum dressing in septic wound treatment].

During the last 2 years, we used the vacuum-sealing technique in more than 300 patients. We have seen several advantages: clean and sterile wound dressing and safe wound healing. In addition, we have used the technique successfully in infected areas in combination with alloplastic material. The only problem we found is the dependency on operating theatres and a vacuum. We think the technique should be recommended for septic wounds in surgery.

Combined Modality Therapy↗

[Lidocaine tape (Penles--a dressing tape based on 60% lidocaine--) reduces the pain of postherpetic neuralgia].

The treatment of postherpetic neuralgia (PHN) by topical administration of local anesthetics has a number of drawbacks. Lidocaine tape (Penles) is a 15 cm2 dressing tape based on 60% lidocaine used to anesthetize skin when an intravenous catheter is inserted. This study aims to evaluate the analgesic efficacy of lidocaine tape in patients with PHN by comparing the results with those of surgical drape (Tegaderm). In a single-blind, two session study, lidocaine tape or surgical drape was applied to the affected skin of 10 patients. In one session, lidocaine tape was applied to the painful skin area, and in another, surgical drape was applied to the same area. Pain score and side effects were measured over 12 hours. Pain score was reduced at measurements taken starting from 1 hour after lidocaine tape application (P < 0.05). Lidocaine tape induced minor side-effects, erythema in a patient and increase in pain in another patient. In conclusion, lidocaine tape is effective for relief of PHN.

Aged↗

Cutaneous Rhizopus infection. Occurrence as a postoperative complication associated with an elasticized adhesive dressing.

A 29-year-0ld woman in good health except for scoliosis suffered severe sequelae during the postoperative course for placement of a Harrington rod. A cutaneous Rhizopus infection in and about the incision site was attributed to the use of a contaminated elasticized adhesive (Elastoplast) dressing. The comtamination was established as a nosocomial problem, which is extremely difficult to control. The extent of the infection, subsequent long recovery course, and remarkable sequelae make this case unusual.

Adult↗

Preparation and characterization of chitin beads as a wound dressing precursor.

Chitin was dissolved in N, N-dimethylacetamide/5% lithium chloride (DMAc/5%LiCl) to form a 0.5% chitin solution. Chitin beads were formed by dropping the 0.5% chitin solution into a nonsolvent coagulant, ethanol. The beads were left in ethanol for 24 h to permit hardening, consolidation, and removal of residual DMAc/5%LiCl solvent in order to give spherical chitin beads uniform size distribution. The ethanol-gelled chitin beads had an average diameter of 535 microm. The chitin beads were subsequently activated in 50% (w/v) NaOH solution and reacted with 1.9 M monochloroacetic acid/2-propanol solution to introduce a carboxymethylated surface layer to the chitin beads. The bilayer character of the surface-carboxymethylated chitin (SCM-chitin) beads was verified by Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and confocal microscopy. The bilayered SCM-chitin beads were found to absorb up to 95 times their dry weight of water. These SCM-chitin beads have potential as a component of wound dressings.

Algorithms↗

Pressure measurements beneath below-knee amputation stump bandages: elastic bandaging, the Puddifoot dressing and a pneumatic bandaging technique compared.

Elastic wrap bandaging is unreliable and dangerous in terms of pressure and pressure distribution (tourniquet effect). The Puddifoot dressing is safe and is less likely to produce a tourniquet effect. The range of pressures it produces is low--possibly too low to achieve ideal moulding or oedema control. A pneumatic bandage achieved a relatively narrow range of pressures between capillary and arteriolar blood pressures. It minimized tourniquet effects and differences between skilled and unskilled bandagers. It is sufficiently promising to merit development.

Amputation Stumps↗

Foam elastomer dressing in the management of open granulating wounds: experience with 250 patients.

Our experience in the use of a silicone foam elastomer in the dressing of granulating wounds is described in a series of 250 patients. Among the types of wound treated were a pilonidal sinus, abdominal, perianal and perineal wounds, bed sores and diabetic gangrene of the foot. The technique has marked advantages over conventional packing of wounds in relation to patient comfort and saving of nursing time in hospital, and can eliminate the need for district nursing care in the domiciliary situation. Details of preparation, application and surgical management are given.

Adult↗

The use of silastic foam dressing in the treatment of advanced hidradenitis suppurativa.

We describe our experience in the use of a Silastic foam dressing in 17 patients undergoing radical excision of hidradenitis suppurativa whose wounds were allowed to heal by granulation. Axillary excisions were carried out in 9 patients and perineal excisions in 8 patients. The technique permits adequate excision of the disease and results in a cosmetically acceptable scar, superior to that obtained by skin grafting and with little limitation of movement. It avoids the pain of conventional management of granulating wounds by gauze packing. It is considered that this method of management is superior in most respects to other techniques used to manage the defect resulting from adequate excision of hidradenitis.

Adolescent↗

Physical and biological properties of a new synthetic amino acid copolymer used as wound dressing.

A new synthetic amino acid copolymer has been evaluated as wound covering. It is permeable to water vapor in the region of 4.1 kg/m2/24 h, it does not allow microbial proliferation after in vitro inoculation, it is impermeable to bacteria, and is stable and flexible. In vivo experiments were designed to provide qualitative and quantitative evaluation on its possible use as a skin substitute in full-thickness skin excision in the guinea pig. Two excisions, approximately 12-14 cm2 were performed on each side of the spine, leaving the panniculus carnosus. One site was treated with the membrane, the other with gauze. Each animal served as its own control. Photographs with a fixed focal-length camera were taken in identical conditions for all wounds immediately and 7, 14, and 21 days after excision. They were analyzed by planimetry. Histological studies were performed at 7, 14, and 21 days. The rate of healing between 0 and 21 days of the wounds treated with the copolymer membrane was significantly accelerated in comparison with wounds covered with a dry dressing (p less than 0.05). This increased epithelialization rate was confirmed by histology, which also suggested a reduction of the inflammatory response of the wound. In vivo biodegradation studies were also performed by subcutaneous implantation in the rat followed at 15, 30, 60, and 90 days by histology and physicochemical analyses. The results demonstrate that the membrane is not biodegradable.

Animals↗

Radiation synthesis of poly(N-vinyl-2-pyrrolidone)-kappa-carrageenan hydrogels and their use in wound dressing applications. I. Preliminary laboratory tests.

Poly(N-vinyl-2-pyrrolidone)-kappa-carrageenan hydrogels (PVP-KC) were prepared by irradiating the mixtures of aqueous solutions of PVP, KC, potassium chloride, and poly(ethylene glycol) by gamma-rays at different doses. Their preliminary laboratory tests were evaluated to identify their usability in wound dressing applications. For investigation of the effect of components on the gelation of PVP, sol-gel analyses were made and gel fractions of the hydrogels were determined. Mechanical experiments were conducted for both unirradiated and irradiated samples. For investigation of the fluid uptake capacity of the hydrogels, swelling experiments were performed in pseudo-extracellular fluid solution at various temperatures. Acidity/alkalinity (pH) and electrical conductivity tests were achieved from aqueous extracts of hydrogels, and bioadhesion strength of the hydrogels was investigated on human skin.

Bandages↗

Electrospun nanofibrous polyurethane membrane as wound dressing.

Produced via electrospinning, polyurethane membrane, which has a unique property, has been of interest in medical fields. Electrospinning is a process by which nanofibers can be produced by an electrostatically driven jet of polymer solution. Electrospun fibers are collected in the form of membranes. The porous structured electrospun membrane is particularly important for its favorable properties: it exudates fluid from the wound, does not build up under the covering, and does not cause wound desiccation. The electrospun nanofibrous membrane shows controlled evaporative water loss, excellent oxygen permeability, and promoted fluid drainage ability, but still it can inhibit exogenous microorganism invasion because its pores are ultra-fine. Histological examination indicates that the rate of epithelialization is increased and the dermis becomes well organized if wounds are covered with electrospun nanofibrous membrane. This electrospun membrane has potential applications for wound dressing based upon its unique properties.

Animals↗

A simple method for processing NMR spectra in which acquisition is delayed: applications to in vivo localized 31P NMR spectra acquired using the DRESS technique.

It is a requirement of many localized NMR spectroscopy experiments, such as DRESS, that collection of the free induction decay be delayed a significant time after the middle of the last pulse. This leads to distortion of the spectra and seriously impedes the retrieval of quantitative information from these spectra. A technique is described in which a correction function is derived from the convoluted spectrum and is used to produce a spectrum with much reduced distortion from which quantitative information may easily be obtained by conventional techniques.

Brain Chemistry↗

Risk posed to honeybees (Apis mellifera L, Hymenoptera) by an imidacloprid seed dressing of sunflowers.

In a greenhouse metabolism study, sunflowers were seed-treated with radiolabelled imidacloprid in a 700 g kg-1 WS formulation (Gaucho WS 70) at 0.7 mg AI per seed, and the nature of the resulting residues in nectar and pollen was determined. Only the parent compound and no metabolites were detected in nectar and pollen of these seed-treated sunflower plants (limit of detection < 0.001 mg kg-1). In standard LD50 laboratory tests, imidacloprid showed high oral toxicity to honeybees (Apis mellifera), with LD50 values between 3.7 and 40.9 ng per bee, corresponding to a lethal food concentration between 0.14 and 1.57 mg kg-1. The residue level of imidacloprid in nectar and pollen of seed-treated sunflower plants in the field was negligible. Under field-growing conditions no residues were detected (limit of detection: 0.0015 mg kg-1) in either nectar or pollen. There were also no detectable residues in nectar and pollen of sunflowers planted as a succeeding crop in soils which previously had been cropped with imidacloprid seed-treated plants. Chronic feeding experiments with sunflower honey fortified with 0.002, 0.005, 0.010 and 0.020 mg kg-1 imidacloprid were conducted to assess potential long-term adverse effects on honeybee colonies. Testing end-points in this 39-day feeding study were mortality, feeding activity, wax/comb production, breeding performance and colony vitality. Even at the highest test concentration, imidacloprid showed no adverse effects on the development of the exposed bee colonies. This no-adverse-effect concentration of 0.020 mg kg-1 compares with a field residue level of less than 0.0015 mg kg-1 (= limit of detection in the field residue studies) which clearly shows that a sunflower seed dressing with imidacloprid poses no risk to honeybees. This conclusion is confirmed by observations made in more than 10 field studies and several tunnel tests.

Alkenes↗

In vitro evaluation of the antibacterial activity of Arctium lappa as a phytotherapeutic agent used in intracanal dressings.

The discovery of natural biocomponents from plants with antibacterial activity on endodontic microbiota may lead to new therapies. This study evaluated the antibacterial activity of a phytotherapeutic agent prepared from an ethyl acetate fraction (AcOEt) extracted from Arctium lappa. This agent was compared with calcium hydroxide as an intracanal dressing. Twenty-seven maxillary canines were instrumented, sterilized and inoculated with a mixed bacterial suspension of Pseudomonas aeruginosa, Escherichia coli, Lactobacillus acidophilus, Streptococcus mutans and Candida albicans. The teeth were divided into three groups and their canals filled with: group 1, calcium hydroxide and propylene glycol; group 2, a paste containing AcOEt fraction of A. lappa and propylene glycol; group 3, propylene glycol (control). At 7, 14 and 30 days, three teeth from each group were opened and a paper point was placed in the root canal for 5 min. The paper points were transferred to Petri dishes with Brain Heart Infusion (BHI). The bacterial growth was classified. Mild bacterial growth was found in group 1 at all time intervals; in group 2 there was severe growth at 7 days, but no growth at 14 and 30 days. The phytotherapeutic agent extracted from an AcOEt fraction of A. lappa inhibited the growth of all the microorganisms in this study.

Acetates↗

Frozen storage of dressed and pre-fried portions of minced sardine muscle.

The aim of this work was to study the modifications occurring during frozen storage (-18 degrees C) of dressed and pre-fried portions of minced muscle of the sardine (Sardina pilchardus). Various samples were prepared in order to discover the effect on the samples of different preparatory frying times, the influence of vacuum-packing and the possible alterations in the state of preservation of the portions due to the presence of anti-oxidant agents. The results indicate that the presence of tocopherols did not have the expected effect on rancidity; it reduced the degree of rancidity reached during storage only slightly, while texture with respect to the control sample remained the same. Although the washed-muscle sample presented minimum rancidity values, the tasting panel awarded it the lowest score for acceptability. In contrast, the vacuum-packed sample presented less toughness and rancidity and was considered more acceptable, in the light of which this latter procedure would appear to be the best suited for frozen storage for pre-fried battered sardine portions.

Animals↗

Feedlot manure top dressing for irrigated pasture: good agricultural practice or a health hazard?

Top dressing a spray-irrigated pasture of orchard-grass with 125 t/ha of overwintered beef feedlot manure almost doubled the yield of dry forage: 6353 kg/ha vs. 3578 kg/ha for the plots receiving no manure. Even at this rate of application, the manure did not contaminate the irrigated grass with enteropathogenic bacteria after irrigation. When the grass became contaminated through the use of fecally polluted irrigation water, the fecal coliforms, indicator organisms with survival characteristics similar to the salmonellae, were effectively eliminated from the grass after exposure to 65 hr of bright sunlight.

Agriculture↗