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PubMed1984

Complement fixing antibodies to Orungo virus in food animals of Northern Nigeria.

In 1981 serum samples were collected from 1106 domestic ruminants in seven different locations in Kaduna and Sokoto States of Northern Nigeria. These states represent the part of the country where there has been no reports of clinical disease or antibody to Orungo virus. Orungo virus antibodies were detected in 26.7% of the sheep, 6.8% of the cattle, 30.7% of the goats and 37.5% of the camels tested by the complement fixation method. The antibody titer in the various positive samples were also determined. Hitherto, Orungo virus infection has not been reported in goats and camels in this country.

Animals↗
PubMed1983

Eimeria cameli (Henry and Masson, 1932) Reichenow, 1952: redescription and prevalence in the Eastern Province of Saudi Arabia.

Eimeria cameli is reported for the first time from Saudi camels. This parasite occurs throughout the year but is commonest during September. Of 960 camels of which fecal samples were examined 14% harboured oocysts. The oocyst of E. cameli measures 86-108 X 61-86 microns and is dark brown to black in the tissues and when passed out in the feces. Giant schizonts are seen in the jejunum and not in the abomasum as previously reported. This and other morphological features departing from previous descriptions are discussed in the text.

Animals↗
PubMed1978

Identification of Echinococcus granulosus strains by enzyme electrophoresis.

Hydatid cysts were collected from camels, horses, oxen and sheep in various geographical locations. Extracts from them were compared for electrophoretic forms of glucose phosphate isomerase. All horse cyst extracts had identical zymogram patterns. These differed markedly in mobility from those of cattle and sheep cysts, all of which were identical to one another. Extracts from camels were compared indirectly and may show a third type of pattern.

Animals↗
PubMed1978

[Position of tylopoda in the system of placental mammals].

Anatomical features of the Tylopoda are determined to a great extent by the convergence with the Ruminantia. The dental system adapted to the double mastification of plant food. The convergent character of the structure of limbs follows from the reduction of carpal and tarsal bones in the Tylopoda and their presence in the Ruminantia. The Ruminantia have no mandibular angular processes which are present in the Tylopoda. In the Tylopoda, the foramina in the transversal processes of cervical vertebrae from a canal by which the vertebral artery opens in the brain. In the Ruminanta there is no such a canal. The embryonic development of camels is characterized by the promitive features of provisory organs. The mating of camels occurs in winter, a feature probably showing that they have preserved the reproduction cycle of the Southern Hemisphere animals.

Anatomy, Comparative↗
PubMed1995

Wesselsbron virus antibody in domestic animals in Nigeria: retrospective and prospective studies.

Retrospective and prospective serological surveys to determine the prevalence of Wesslsbron (WSL) virus infections in animal populations were carried out in different vegetational zones in Nigeria. Sera from 1,492 animals comprising 292 camels, 81 horses, 4 donkeys, 320 cattle, 235 sheep, 260 goats, 114 pigs, 101 dogs and 85 domestic fowls were assayed by haemagglutination-inhibition (HI) test for presence of antibodies to WSL virus and other flavivirus antigens: Yellow Fever (YF), Potiskum (POT), Banzi (BAN), Uganda S (UGS) and West Nile (WN) viruses. Four hundred and eighty one (32%) of the total sera tested were positive for the presence of flavivirus antibodies. The prevalence rates among animals varied with species and vegetational zones of the country. The highest prevalence was noted in animals from a swamp forest zone and was higher among camels, horses, donkeys and sheep when compared with goats, pigs and fowls in different zones. Although monotypic reactions with WSL virus antigen were observed in positive sera, the majority of the WSL virus positive sera cross-reacted with more than two other flavivirus antigens. Serological cross-reactions were most extensive in WSL virus positive horse sera. A ten month sentinel survey among 28 cattle, 68 sheep and 30 goats revealed considerable activity of WSL virus in Nigeria. Of these, 11 cattle and 12 sheep showed antibody conversion to WSL virus antigen. None of the goats seroconverted. Although, there are no records of outbreak of WSL disease in Nigeria, this study revealed that WSL virus is actively circulating among livestock populations in this environment. Flavivirus nucleotide data are needed for final determination of genetic relatedness in this group of viruses.

Animals↗
PubMed1995

Potential hazards associated with influenza virus vaccines.

There is general agreement that human reassortant vaccine strains should be used only for the preparation of the viral glycoproteins (split vaccine), and not in toto after UV-inactivation. Live vaccines with lowered pathogenicity obtained by reassortment between human and avian strains may carry a risk of causing epizootics in other species, even though they are useful for the host for which they were designed. It has been argued that this dangerous situation is avoided when both parent strains are of human origin. For this reason, cold-adapted human master strains are used for reassortment with the most recent isolates from cases of human influenza. There is, however, convincing evidence that a reassortant between two human strains has caused severe epizootics among camels, which were not regarded as natural hosts for influenza A viruses. This sudden appearance of a reassortant camel virus had a precedent in experiments in which, starting from parent strains that are non-pathogenic for mice, highly pathogenic reassortants for this species were created. Safety requirements for cold-adapted reassortants must therefore take account of the fact that these new strains may have a high pathenogenicity for other species.

Animals↗
PubMed1975

Leptospirosis in Ethiopia: a serological survey in domestic and wild animals.

Seven hundred and fifty-eight serum samples from domestic and wild animals in Ethiopia were tested for leptospiral antibodies by the microscopic agglutination test. The following percentages of seropositivity were obtained: horse (91.3 per cent), cow (70.7 per cent), pig (57.1 per cent), goat (47.3 per cent), sheep (43.4 per cent), camel (15.4 per cent), and dog (8.3 per cent). All 54 samples from birds and wild animals were seronegative. Most of the positive sera exhibited reaction to more than one serotype and antibodies to serotype butembo were predominant in sera of the majority of the examined species. However, grippotyphosa, rather than butembo was the predominant reacting serotype in camels and dogs. It is concluded that leptospirosis is of significant prevalence in domestic animals in Ethiopia and may constitute an occupational health hazard to man.

Agglutination Tests↗
PubMed1993

Report of the Thirteenth Meeting of the OIE Ad hoc Group on Non Tsetse-Transmitted Animal Trypanosomoses.

There is increasing interest in many parts of the world in trypanosomoses other than those transmitted by tsetse flies, as shown by numerous research projects and field studies. The refinement of techniques for studying the behaviour of trypanosomes (techniques of molecular biology) in axenic culture or in the parasitised host has led to progress in diagnosis and immunology, and a rational approach to chemotherapy and chemoprophylaxis of these infections. Field trials of enzyme-linked immunosorbent assays in Africa, Asia and South America have shown that these tests may now be regarded as reliable in demonstrating antibodies or antigens for Trypanosoma evansi infection in buffalo, cattle and camels, and for mono-infection with T. equiperdum in equines. However, it is not yet possible to differentiate reliably between infections with T. evansi and T. equiperdum in equines. The card agglutination trypanosomosis test (CATT) has been adapted to T. evansi infection and can also be recommended. Immunosuppression induced by T. evansi infection inhibits the immune response to vaccination against Pasteurella haemolytica. In areas freed from tsetse flies (Cameroon, Central African Republic, Zambia) it has been observed that Trypanosoma vivax can be transmitted mechanically by other biting insects, which are at present being identified. Research on trypanocides has led to the toxic factor for Trypanosoma brucei or T. equiperdum present in human or simian serum being localised to the high density lipoprotein of serum lipoproteins. Various derivatives are being tested under laboratory conditions, and the efficacy of some (e.g. ronidazole) is being checked at present, while others are ready to pass to the development stage (e.g. IMOL 881). Melarsomine, already available commercially (as Cymelarsan) for the treatment of T. evansi infection in camels, is being studied for possible use in other species of animals.

Animals↗
PubMed1993

Evaluation of the in vitro transformation technique to distinguish Trypanosoma evansi from cyclically transmitted Trypanozoon stocks.

In order to initiate transformation into procyclic forms, bloodstream trypanosomes, were transferred to semi-defined medium at 27 degrees C. All stocks previously classified as Trypanosoma brucei rhodesiense, T.b. gambiense and T.b. brucei transformed into procyclic forms. None of the 31 characterized T. evansi stocks transformed into procyclic forms, but died between day 4 and day 6 in culture. On the other hand, 3 out of 64 stocks of monomorphic brucei subgroup field isolates from camels transformed into procyclic forms, confirming the existence of T.b. brucei infection in camels kept close to tsetse belts.

Animals↗
PubMed1995

Studies on Chrysomyia albiceps (Wiedemann) one of the most important carrion flies in Egypt.

Chrysomyia albiceps often feeds to repletion and then regurgitates a portion of the food, a behaviour that heightens its importance as a mechanical vector of enteric pathogens. Pupae of this fly are susceptible to the attack of the parasitic wasp, Nasonia vitripennis in the laboratory as well as in nature at different underground depths where they pupate. The fly needs to feed on proteinaceous meals to start and complete its ovarian development. Liver, beef, camel, chicken, rabbit and fish meat were tested. Of the 6 tested diets, liver proved to be the most advantageous as 98% pupation was obtained, while with beef, camel, chicken, rabbit and fish 95.2%, 95.9%, 90.9%, 89.1% and 86% pupation, respectively, were obtained. Field observations revealed that Chrysomyia albiceps prefers to breed in big carcasses over small ones as the latter contain less moisture or amount of food and are subjected to desiccation and consumption before the full growth of larvae especially during the hot and dry months. Anatomical studies of the females attracted to a piece of meat in laboratory and outdoors experiments showed that the degree of ovarian development is variable including completely undeveloped ovaries, sexually mature ones and different stages of ovarian development. So, attraction to meat fulfill to important functions: nutrition and oviposition. Feeding visits are short in duration (6.2 +/- 2.1 min) and behaviourally simple. The oviposition visits last from 40-60 min with more complex behaviour which can be divided into the time before, during and after oviposition. Females in selecting a place for oviposition, preferred the softer places of the fresh meat; round its lower moist edge where much liquid food is present and where the females oviposit in groups at one location. When a female began to oviposit, it was joined by others that located the eggs with their ovipositors and started to oviposit where a clumped irregular egg masses are deposited.

Animals↗
PubMed1996

In vivo and in vitro characterization of two camelpoxvirus isolates with decreased virulence.

Two camelpoxvirus (CPV) strains isolated from camels with generalized skin-disease were serially passaged on Vero cells. Various phenotypic properties were investigated in vitro and in vivo and compared with those of the corresponding wildtype strains. In many aspects no differences were observed. However, in a mouse model both passaged strains proved to be highly attenuated. In addition, both strains failed to replicate in a cell line derived from camel skin cells. Comparison of physical maps established for enzymes HindIII and Xhol revealed deletions accounting for a total of 22 kbp in one attenuated strain. In the second strain only minor alterations were noted.

Animals↗
PubMed1996

[Preliminary study of the safety and immunogenicity of the attenuated VD47/25 strain of camelpoxvirus].

The safety and immunogenicity of the attenuated VD47/25 strain of camelpoxvirus were tested on 30 camel calves in Mauritania. Post-inoculation clinical symptoms were absent during the 40 days of observation. Serum samples collected during this period showed low levels of neutralizing antibodies (1/4-1/16). In vivo titration of a virulent strain of camelpoxvirus in vaccinated camels and control animals enabled the calculation of the PD50 (50% protective dose) which contained the equivalent of 10(3.7) TCID50 (50% cell culture infective dose). Other studies are still required to determine the dose of this vaccine needed to protect 95% of vaccinated animals.

Animals↗
PubMed1997

Cigarette promotional items in public schools.

OBJECTIVES: To assess the prevalence of ownership of cigarette promotional items (CPIs) by rural northern New England students and to examine the association between CPI ownership and smoking behavior. DESIGN AND SETTING: Voluntary, self-administered survey of 1265 sixth- through 12th-grade students representing 79% to 95% of all students attending 5 rural New Hampshire and Vermont public schools in October 1996. We examined the association between ownership of a CPI and smoking behavior through regression models and conducted a sensitivity analysis on the findings. MAIN OUTCOME MEASURES: Adjusted odds of being a smoker (lifetime use of > or = 100 cigarettes) and, among never smokers and experimental smokers, adjusted cumulative odds of having higher levels of smoking uptake given CPI ownership. RESULTS: One third of students owned a CPI. Prevalence of ownership did not vary by grade or sex, but was higher among poor-to-average school performers (45.0% vs 21.0% for excellent school performers, P < .001) and children whose friends and family members smoked (43.4% vs 13.8% for students with no family members or friends smoking, P < .001). Cigarette promotional items included articles of clothing (T-shirts, hats, backpacks, and jackets), smoking paraphernalia (lighters and ashtrays), camping gear, and electronics. More than half of CPIs (58.2%) bore the Marlboro logo, and almost one third (31.7%) bore the Camel logo. These items were obtained directly from catalogs or vendors 22.4% of the time. Whereas only 4.5% of students reported bringing a CPI to school with them the day of the survey, 44.5% reported seeing such an item at school the day of the survey. After controlling for confounding factors, such as having friends who smoke, students who owned CPIs were 4.1 times more likely to be smokers than those who did not own CPIs (95% confidence interval [CI], 3.1-5.5). Never and experimental smokers (n = 1008) who owned CPIs were more likely to be in a higher category on the smoking uptake index in grades 6 (cumulative odds ratio [OR = 5.7, 95% CI, 1.9-16.8), 7 (OR = 1.8, 95% CI, 0.9-3.7), 8 (OR = 2.3, 95% CI, 1.1-4.8), and 9 (OR = 2.1, 95% CI, 1.1-3.9), periods when children are most vulnerable to initiating cigarette use. A sensitivity analysis indicated that an unmeasured confounder of CPI ownership and smoking was unlikely to alter our conclusions. CONCLUSIONS: Cigarette promotional items are owned by one third of students in these rural northern New England schools. These items are highly visible in the public school setting, and their ownership is strongly associated with initiation and maintenance of smoking behavior. These data lend support to a ban on CPIs to be included in US Food and Drug Administration regulations to prevent tobacco use among US youth.

Adolescent↗
PubMed1998

Tobacco industry promotion of cigarettes and adolescent smoking.

CONTEXT: Whether tobacco advertising and promotion increases the likelihood that youths will begin smoking has important public policy implications. OBJECTIVE: To evaluate the association between receptivity to tobacco advertising and promotional activities and progress in the smoking uptake process, defined sequentially as never smokers who would not consider experimenting with smoking, never smokers who would consider experimenting, experimenters (smoked at least once but fewer than 100 cigarettes), or established smokers (smoked at least 100 cigarettes). DESIGN: Prospective cohort study with a 3-year follow-up through November 1996. SETTING AND PARTICIPANTS: A total of 1752 adolescent never smokers who were not susceptible to smoking when first interviewed in 1993 in a population-based random-digit dial telephone survey in California were reinterviewed in 1996. MAIN OUTCOME MEASURE: Becoming susceptible to smoking or experimenting by 1996. RESULTS: More than half the sample (n=979) named a favorite cigarette advertisement in 1993 and Joe Camel advertisements were the most popular. Less than 5% (n=92) at baseline possessed a promotional item but a further 10%(n=172) were willing to use an item. While having a favorite advertisement in 1993 predicted which adolescents would progress by 1996 (odds ratio [OR] = 1.82; 95% confidence interval [CI], 1.04-3.20), possession or willingness to use a promotional item was even more strongly associated with future progression (OR=2.89; 95% CI, 1.47-5.68). From these data, we estimate that 34% of all experimentation in California between 1993 and 1996 can be attributed to tobacco promotional activities. Nationally, this would be over 700000 adolescents each year. CONCLUSION: These findings provide the first longitudinal evidence to our knowledge that tobacco promotional activities are causally related to the onset of smoking.

Adolescent↗
PubMedDate not supplied

Characterization of sialidase from bloodstream forms of Trypanosoma vivax.

Sialidase (EC: 3.2.1.18) from Trypanosoma vivax (Agari Strain) was isolated from bloodstream forms of the parasite and purified to apparent electrophoretic homogeneity. The enzyme was purified 77-fold with a yield of 32% and co-eluted as a 66-kDa protein from a Sephadex G 110 column. The T. vivax sialidase was optimally active at 37 degrees C with an activation energy (E(a)) of 26.2 kJ mole(-1). The pH activity profile was broad with optimal activity at 6.5. The enzyme was activated by dithiothreitol and strongly inhibited by para-hydroxy mercuricbenzoate thus implicating a sulfhydryl group as a possible active site residue of the enzyme. Theenzyme hydrolysed Neu5Ac2,3lac and fetuin. It was inactive towards Neu5Ac2,6lac, colomic acid and the gangliosides GM1, and GDI. Initial velocity studies, for the determination of kinetic constants with fetuin as substrate gave a V(max) of 142.86 micromol h(-1) mg(-1) and a K(M) of 0.45 mM. The K(M) and V(max) with Neu5Ac-2,3lac were 0.17 mM and 840 micromole h(-1) mg(-1) respectively. The T. vivax sialidase was inhibited competitively by both 2,3 dideoxy neuraminic acid (Neu5Ac2,3en) and para-hydroxy oxamic acid. When ghost RBCs were used as substrates, the enzyme desialylated the RBCs from camel, goat, and zebu bull. The RBCs from dog, mouse and ndama bull were resistant to hydrolysis.

Animals↗
PubMed1994

The Osborn wave of hypothermia in normothermic patients.

The Osborn wave (also referred to as "the J wave," "the J deflection," or "the camel's hump") is a distinctive deflection occurring at the QRS-ST junction of approximately 80% of hypothermic patients (core body temperature < or = 95 degrees F). Generally, the amplitude and duration of Osborn waves are inversely related to core temperature. We report on eight normothermic patients whose 12-lead electrocardiograms demonstrated QRS-ST junction notches similar to those seen in hypothermia. These data support the concept that the Osborn wave is not pathognomonic of hypothermia.

Adult↗
PubMed2007

Antibody constructs in cancer therapy: protein engineering strategies to improve exposure in solid tumors.

Whereas over 85% of human cancers are solid tumors, of the 8 monoclonal antibodies (mAbs) currently approved for cancer therapy, 25% are directed at solid tumor surface antigens (Ags). This shortfall may be due to barriers to achieving adequate exposure in solid tumors. Advancements in tumor biology, protein engineering, and theoretical modeling of macromolecular transport are currently enabling identification of critical physical properties for antitumor Abs. It is now possible to structurally modify Abs or even replace full Abs with a plethora of Ab constructs. These constructs include Fab and Fab'(2) fragments, scFvs, multivalent scFvs (e.g., diabodies and tribodies), minibodies (e.g., scFv-CH3 dimers), bispecific Abs, and camel variable functional heavy chain domains. The purpose of the article is to provide investigators with a conceptual framework for exploiting the recent scientific advancements. The focus is on 2 properties that govern tumor exposure: 1) physical properties that enable penetration of and retention by tumors, and 2) favorable plasma pharmacokinetics. It is demonstrated that manipulating molecular size, charge, valence, and binding affinity can optimize these properties. These manipulations hold the key to promoting tumor exposure and to ultimately creating successful Ab therapies for solid tumors.

Antibodies↗