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[Shared development of printed material as a health education strategy with public school employees in Rio de Janeiro State].

This article describes the process and results of an experience with preparing a mural entitled A Day in the Life of the School Lunch Cook, conducted with public school employees in the State of Rio de Janeiro by a team of researchers and students from the Specialization Course in Workers' Health at the Center for Studies on Workers' Health of the National School of Public Health, Oswaldo Cruz Foundation, in partnership with the School Employees' Union. "Health workshops" based on participatory research were used to raise questions related to daily work by school lunch cooks and kitchen staff. Produced to share the results of workshops with other schools, the mural is highly meaningful in terms of the reality of a specific segment of public employees and is intended as food for thought. Based on the Paulo Freire popular education model, six meetings were held, following a script for the production of an educational leaflet including the selection of contents, the communications approach, and the style and format of the leaflet. The creativity in the images prepared by the school lunch employees based on actual experiences from their daily work made the workshops an enriching moment of exchange, in which the participants shared their ideas, values, beliefs, feelings, and life histories.

Brazil↗

Diabetes portrayals in North American print media: a qualitative and quantitative analysis.

OBJECTIVES: This study investigated how media coverage has portrayed diabetes as newsworthy. METHODS: The quantitative component involved tabulating diabetes coverage in 2 major Canadian newspapers, 1988-2001 and 1991-2001. The qualitative component focused on high-profile coverage in 2 major US magazines and 2 major Canadian newspapers, 1998-2000. RESULTS: Although coverage did not consistently increase, the quantitative results suggest an emphasis on linking diabetes with heart disease and mortality to convey its seriousness. The qualitative component identified 3 main ways of portraying type 2 diabetes: as an insidious problem, as a problem associated with particular populations, and as a medical problem. CONCLUSIONS: Overall, the results suggest that when communicating with journalists, researchers and advocates have stressed that diabetes maims and kills. Yet even when media coverage acknowledged societal forces and circumstances as causes, the proposed remedies did not always include or stress modifications to social contexts. Neither the societal causes of public health problems nor possible societal remedies automatically received attention from researchers or from journalists. Skilled advocacy is needed to put societal causes and solutions on public agendas.

Attitude to Health↗

Parallel production of oligonucleotide arrays using membranes and reagent jet printing.

Here, we present a low-cost method to produce compact arrays using microporous materials and reagent jetting. Oligonucleotides are immobilized on membrane sheets as a series of lines. The membrane sheet is then rolled and bound, and the spiral structure is cut like a "jelly roll" to produce identical arrays. The spiral arrays behave much like larger formats using membranes, and hybridization detection can be accomplished using standard signal-generation mechanisms. The method is particularly useful for producing identical arrays from pre-synthesized oligonucleotides.

Equipment Design↗

LNA-modified oligodeoxynucleotide hybridization with DNA microarrays printed on nanoporous membrane slides.

We report a robust method for the detection of hybridization events using a microarray-based assay on a nanoporous membrane platform. The technique is characterized by a hybridization time of only 1 hour and uses Cy5-labeled, 7-mer oligodeoxynucleotide probes modified with locked nucleic acid (LNA) nucleotides. We show that the volume of the DNA spotted onto a nanomembrane can be reduced to approximately 4 nL with detectable signal intensity. Moreover, the amount of the DNA target could be reduced to 4 fmol. The described approach could dramatically increase the throughput of techniques based on sequencing by hybridization, such as oligofingerprinting, by decreasing the total number of probes that are needed for analysis of large clone sets and reduction of the sample/reagent consumption. The method is particularly advantageous when numerous hybridization-based assays must be performed for characterization of sample sets of 100,000 or more.

Membranes, Artificial↗

Environmental print as strategy for developmental literacy of young atypical children.

12 children with handicapping problems were taught various strategies during a two-year period. These programs, designed to enrich their appreciation and awareness of the sounds of letters and how they are used, increased the children's attention span, parental participation with children and teacher, and promoted active involvement in reading.

Child↗