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Results for “Complement Fixation Tests”

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At least 1,531 records · Page 85Linked to original sources
PubMed1987

Influenza A and B virus IgG and IgM serology by enzyme immunoassays.

Enzyme immunoassays (EIA) for IgG and IgM antibodies against influenza A and B virus are described. One hundred and seven subjects with a clinical diagnosis of acute respiratory infection (influenza, bronchitis or pneumonia) were selected for this study during two epidemics of influenza A which occurred in Finland in 1983 and 1985. Paired sera and nasopharyngeal secretions were obtained from all subjects. The sera were tested for influenza A and B antibodies by IgG and IgM EIAs and by complement fixation tests. The nasopharyngeal secretions were tested by an indirect EIA for influenza A and B antigens. The IgG EIA was found to be better than complement fixation for the diagnosis of influenza A infections: only 22% of the significant increases detected by this test were also positive by complement fixation. The additional contribution of the IgM EIA to the number of positives was minimal. It was also found that testing a single 1/1000 dilution of serum for influenza A and 1/100 dilution for influenza B in the IgG EIA gave as many positives as the conventional method of testing several dilutions.

Adult↗
PubMed1978

Enzyme-linked immunosorbent assay for measurement of serological response to respiratory syncytial virus infection.

An enzyme-linked immunosorbent assay was applied to the detection of serum antibodies against respiratory syncytial virus. The end points of the various sera tested in the assay were approximately 100 times higher than in the complement-fixation test and 2 to 4 times higher than in the plaque reduction test. In addition, the immunosorbent assay appeared to be more efficient than the plaque reduction and complement-fixation techniques for detecting a serological response in young infants (1 to 6 months old) with serous respiratory syncytial virus lower respiratory disease. The simplicity, sensitivity, and rapidity of the enzyme-linked immunosorbent assay make it a useful tool for immunological studies with respiratory syncytial virus.

Antibodies, Viral↗
PubMed1979

Poliomyelitis, rubella, and dengue antibody survey in Barbados. A follow-up study.

A follow-up study of poliomyelitis, rubella, and dengue antibodies has been made in light of results obtained in a 1972 health and serological survey in Barbados, W.I. Poliomyelitis antibody neutralization tests performed on sera from 307 children under age 15 using overnight serum/virus mixtures on microtiter plates at low serum dilutions revealed the absence of polio antibody at 1:2 dilution in 13.7% for type 1, 6.5% for type 2, and 14.3% for type 3 virus. A significant correlation of the presence or absence of poliomyelitis antibody to types 2 and 3 was seen with the response to immunization histories. Forty-three of 49 girls (88%) given rubella vaccine (RA 27/3) in 1972 had demonstrable haemagglutination-inhibition antibody 4 years later. Neutralization tests for dengue antibody confirmed the results of the complement-fixation tests and indicated that type 2 was probably the sole infecting strain.

Adolescent↗
PubMed1975

Viral hepatitis, type A. Identification by specific complement fixation and immune adherence tests.

Hepatitis A antibody was detected by specific immune adherence and complement-fixation tests in a study involving 473 serum specimens from 20 patients who had viral hepatitis, Type A. In all 20 patients who had no detectable immune adherence antibody (less than 1:5) before onset of hepatitis high levels (greater than or equal to 1:1024) developed one to four weeks later, occasionally reaching peak levels exceeding greater than or equal to 1:81,920 several months thereafter. Five to 10 years later the immune adherence antibody levels ranged between 1:640 and 1:20,480. In general, the complement-fixation test was not as sensitive or as specific as the immune adherence test. These findings indicate that the immune adherence test should be a valuable tool for diagnosis, for epidemiologic surveys, for identification of susceptible and immune persons, for quantitative assays of gamma globulin and for identification of hepatitis A virus in attempts to propagate the virus in cell culture.

Animals↗
PubMed1978

On the mechanism of the persistence of lymphocytic choriomeningitis virus in the continuous cell line Detroit-6.

A persistent lymphocytic choriomeningitis virus, noninfectious for mice, was revealed in the continuous human cell line, Detroit-6. The virus was detected in the cell monolayer by an indirect immunofluorescence test and in the cell homogenate by a complement-fixation test. Between 30 and 80% of the cells produced viral antigen in subsequent passages of the culture. Thymidine analogues (BrDU and IDU) stimulated the synthesis of antigen. DNA of the persistently infected cell line was transfected to the mouse lymphoid cell line, L1210, which became persistently infected with the virus.

Antigens, Viral↗
PubMed1983

Prevalence of antibodies to Mycoplasma hyopneumoniae in Iowa swine.

The prevalence of complement-fixing antibodies to Mycoplasma hyopneumoniae was determined in 7,321 sera collected from breeding swine of various ages. Samples were selected randomly in approximate proportion to the number of hogs marketed annually from each of 9 crop-reporting areas in Iowa. Testing was accomplished by means of a Microtiter complement-fixation test. Of the 7,321 sera, 22% had antibody titers of 1:4 or greater to M hyopneumoniae. Of the 597 herds sampled, 60% or 357 had at least 1 animal with a titer of 1:4 or greater. Use of the chi-square association test indicated that animals which were M hyopneumoniae-positive were more often Haemophilus pleuropneumoniae-positive than those that were M hyopneumoniae-negative.

Animals↗
PubMed1982

Ovine contagious epididymitis: a review.

Ovine contagious epididymitis is predominantly associated with Brucella ovis, Actinobacillus seminis and a variety of organisms including Histophilus ovis. Transmission occurs venereally or by homosexual activity and, in the case of Actinobacillus seminis, ewe to lamb transmission is probably important. A chronic infection, mainly in the cauda epididymis may result in formation of spermatic granulomata and a reduction in ram fertility. Eradication of ovine brucellosis can be achieved by serological testing using a complement-fixation test in conjunction with palpation of lesions and removal of reactors. Vaccination to control ovine brucellosis is advocated in some countries. The nomenclature of the group of Gram-negative pleomorphic organisms to which Actinobacillus seminis and Histophilus ovis belong is urgently in need of review. This paper reviews the organisms capable of producing ovine contagious epididymitis, the pathogenesis of the condition as well as methods of diagnosis and control.

Actinobacillus Infections↗
PubMed2005

Validation of fluorescence polarization assay (FPA) and comparison with other tests used for diagnosis of B. melitensis infection in sheep.

Fluorescence polarization assay (FPA) is a new test for the serological diagnosis of Brucella spp. infection in animals. The FPA is validated for the diagnosis of B. melitensis infection in sheep. For this purpose, 166 sera originated from natural infected sheep (verified by culture) and 851 sera originated from healthy animals (reared in areas where B. melitensis was never been isolated) were tested. The optimum cut-off value that offers the highest diagnostic sensitivity (DSn) and diagnostic specificity (DSp) was determined at 87mP with the use of ROC analysis. The DSn and DSp of FPA using this cut-off value are calculated at 97.6 and 98.9% with a 95% confidence interval (CI) of 93.9-99.3% and 98.0-99.5%, respectively. The DSn and DSp of FPA have been assessed also using as positive reference (n=587), sera that gave positive results at least in two tests used for diagnosis of B. melitensis in sheep as Rose Bengal Test (RBT), modified Rose Bengal Test (m-RBT), complement fixation test (CFT), indirect Elisa (i-Elisa) and competition Elisa (c-Elisa) originated from animals reared in flocks infected by B. melitensis. The optimum cut-off value using the above panel of positive reference sera was the same offering a DSn of 95.9% with a 95% CI, 94.0-97.4%, since the DSp remains the same. The DSn and DSp as well as performance, accuracy and agreement of FPA's result were compared with those of other tests used. The accuracy of FPA is very high, similar with that of i-Elisa. FPA is a promising assay, which offers a DSn and accuracy better that of those of the tests currently approved for the diagnosis of B. melitensis in sheep and goats. Due to its simplicity, the sort time that results can be obtained and its accuracy it can be used and improve the laboratory testing capacity as well as the efficacy of the eradication program based on test-and-slaughter policy.

Animals↗
PubMed1994

Hydatid disease of the kidney: evaluation and features of diagnostic procedures.

We discuss 8 patients with hydatid cystic disease of the kidney. Diagnostic procedures included eosinophil count, Casoni's skin test, Ghedini-Weinberg complement fixation test, abdominal x-ray, excretory urogram, ultrasonography and computerized tomography. The combined findings of these different modalities aid greatly in establishing the correct diagnosis.

Adolescent↗
PubMed2000

Risk factors for Brucella spp. infection In dairy cattle farms in Asmara, State of Eritrea.

A cross-sectional study was conducted to identify risk factors for herd infection by Brucella spp. in dairy cattle in the suburbs of Asmara, Eritrea. Data were collected from 64 herds, randomly selected from a total of 99 herds with a minimum herd size of 9 cows. A questionnaire was used to gather data on management, hygiene and herd structure. Serum samples collected from all pregnant heifers, cows and bulls, were screened for Brucella infection by the Rose Bengal test (RBT), and all RBT-positive sera re-tested with the complement-fixation test (CFT) for confirmation. A seropositive herd was defined as one in which at least one animal tested positive in the CFT. There were 23 (36%) positive herds among the 64 studied. Both multiple logistic and multiple betabinomial regression modeling were used to analyze the data. Mixed-breed herds, compared to single (exotic)-breed herds, were found to be independently associated with increased herd seroprevalence (OR=5.2, 95% confidence interval 1. 4-18.7) in the multiple logistic model with the herd infection status as the dependent variable. The importance of this variable was supported by the multiple betabinomial regression model (OR=3.3, 1.4-7.6) with animal-level prevalence within herd as the outcome variable. Both models also revealed the presence of a negative association between seropositivity and cattle stocking density.

Animals↗
PubMed2001

Comparison of an indirect ELISA with the Brucella milk ring test for detection of antibodies to Brucella abortus in bulk milk samples.

An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of Brucella abortus antibodies in bovine bulk milk samples was evaluated. About 31 individual milk samples from B. abortus infected cows were diluted into bulk milk from a brucellosis free herd. Individual milk samples obtained from 96 negative or positive herds to ELISA or Brucella ring test (BRT), were tested by ELISA. All positive cows were bled and serum samples were tested by the complement-fixation test (CFT) which was considered the definitive test. A herd was considered infected if at least, one cow was positive in the CFT. Four samples were negative in the BRT at the dilution 1:10 but positive in the ELISA. For samples positive in both tests, BRT titers ranged from 1:10 to 1:480 while ELISA titers ranged from 1:10 to 1:3200. Using bulk milk samples, the sensitivity of the ELISA (98.1%) was higher than the BRT (72.2%) but the specificity of BRT (90.5%) was not statistically different (P=1.0) from the ELISA (88.1%). The implications of the results for brucellosis control are discussed.

Animals↗
PubMed1977

Heparin induced thrombocytopenia: eight cases with thrombotic-hemorrhagic complications.

Increased heparin tolerance and recurrent thromboembolism which included myocardial infarction (3 patients), pulmonary embolism (2 patients) and complete aorto-iliac occlusion (2 patients), heralded the development of thrombocytopenia between the eighth and twelfth day of heparin therapy in six women and two men. The thrombocytopenia persisted until heparin was discontinued. Bleeding (cerebral hemorrhage) was the initial complication in one patients and occurred in conjunction with thrombotic complications in four other patients. Agglutination absorption testing in one and complement fixation testing in five patients suggested the presence of heparin dependent antiplatelet antibodies. After platelet recovery, four of the eight patients responded to parenteral heparin rechallenge with rapid decreases in their platelet counts. The early recognition of the syndrome with cessation of heparin therapy is imperative for the successful management of afflicted patients.

Adult↗
PubMed1985

Microplate enzyme immuno-assay for detection of platelet antibodies.

A simplified and sensitive enzyme immuno-assay employing microtiter plates as the solid phase carrier for detection of circulating platelet antibodies and bound antiplatelet IgG has been described. The assay was done using fresh as well as frozen platelets and commercially available peroxidase labeled anti-human IgG. The sensitivity of the enzyme immuno-assay was found similar or superior to that of the platelet suspension immunofluorescence test and superior to the lymphocyte cytotoxicity test and the platelet complement fixation test. The use of platelets frozen in the wells of the microtiter plates (stored for up to 17 months at -20 degrees C without loss of antigenicity) facilitates the performance of the test. In addition the small volumes of sera and platelets needed in using microtiter plates makes the assay particularly suitable in testing platelets from thrombocytopenic patients. In one of 31 sera from normal Zwa-negative donors a weak anti-Zwa (P1A1) was found only detectable by enzyme immuno-assay. Alloantibodies were found in 14 of 23 patients suffering from non-haemolytic transfusion reactions. All of three patients with post-transfusion purpura had anti-Zwa antibodies in serum. Anti-Zwa antibodies were demonstrated in the sera from all of 9 Zwa-negative mothers, who had given birth to children with alloimmune neonatal thrombocytopenia. In 8 of 12 Zwa-positive mothers alloantibodies of other specificities were found in the serum. In one case the antibody was a known anti-Baka. Six of 7 patients with autoimmune thrombocytopenic purpura had values of platelet-bound IgG exceeding the normal range and circulating platelet antibodies were seen in 4 patients.

Antigens, Human Platelet↗
PubMed2000

Anaplasmosis control and diagnosis in South Africa.

Anaplasmosis is widespread in South Africa with more than 99% of the total cattle population at risk. Five tick species have been experimentally shown to be capable of transmitting Anaplasma in South Africa. Mechanical transmission through blood contaminated instruments and biting flies also occurs. Vaccination against Anaplasma marginale by administration of an Anaplasma centrale live-blood vaccine has been practiced in this country since 1912. Although generally a mild pathogen, Anaplasma centrale can cause severe clinical reactions following vaccination and also does not afford complete protection against all A. marginale isolates. Anaplasmosis vaccine is routinely available in a deep-frozen form and approximately 220,000 doses of vaccine are sold per annum. Microscopic examination of stained thin blood smears is still the most reliable and cost effective method of confirming a clinical diagnosis of anaplasmosis. Several diagnostic tests, such as the complement fixation tests, card agglutination test, and enzyme-linked immunosorbent assay (ELISA) have been developed to identify carrier cattle. A competitive inhibition ELISA test, based on antibody binding to a recombinant MSP-5 protein conserved among Anaplasma species, is routinely used at this laboratory.

Anaplasmosis↗