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Multivariate analysis of the effects of manganese on the reproductive physiology and behavior of the male house mouse.

Chronic exposure to Mn3O4 in the diet at 1050 ppm Mn retarded the sexual development and lowered reactive locomotor activity levels in male mice. A multivariate analysis of variance indicated that testis, seminal vesicle, and preputial gland weights were significantly smaller as a result of Mn administration. These results support earlier observations of altered locomotor activity levels and reproductive development in male rats in the absence of other signs of toxicity.

Animals↗

Mental and physical distress and high-risk behaviors among reproductive-age women.

OBJECTIVE: To examine the prevalence of mental and physical distress indicators among women of reproductive age and the association of these indicators with cigarette smoking and alcohol use, by pregnancy status. METHODS: The Behavioral Risk Factor Surveillance System data for several years were aggregated across states and weighted for this analysis. Seven measures of self-reported mental and physical distress and general health were examined along with demographic variables. RESULTS: Overall, 6.7% (95% confidence interval [CI] 6.5-6.9) of women reported frequent physical distress, 12.3% (95% CI 12.0-12.6) reported frequent mental distress, 9.9% (95% CI 9.4-10.4) reported frequent depression, 18.4% (95% CI 17.8-19.1) reported feeling anxious, and 34.3% (95% CI 33.5-35.1) reported that they frequently did not get enough rest. At the time of the survey 4.6% of the women were pregnant. Pregnant women were less likely than nonpregnant women to report frequent mental distress. Although there was attenuation of cigarette smoking and alcohol use during pregnancy, those with mental and physical distress were more likely to consume cigarettes and alcohol than were those without such experiences. CONCLUSION: High proportions of reproductive-age women report frequent mental and physical distress. Women experiencing mental and physical distress were more likely to report consuming cigarettes and alcohol than women without such experiences.

Adolescent↗

Influence of gonadal sex hormones on behavioral components of the reproductive hierarchy in naked mole-rats.

Naked mole-rats (Heterocephalus glaber) are fossorial, eusocial rodents that live in colonies which typically include 60-80 individuals. Generally, only one of the females and 1-3 of the males in a colony are reproductives. The reproductives engage in mutual genital nuzzling behavior that is rarely exhibited by subordinates (non-reproductives). Thus, genital nuzzling may represent a mechanism of bonding and/or specific recognition between reproductive individuals. We investigated whether gonadal hormones are involved in the maintenance of genital nuzzling behavior and mating behaviors in isolated pairs of mole-rats and also in established breeding pairs of mole-rats within colonies. We also explored whether sex hormone deprivation would alter the strict partner preference for performance of nuzzling within colonies. Our results indicate (a) considerable variation between pairs in the frequency of nuzzling, (b) a reduction in the frequency of nuzzling following castration of the male and restoration of the 'baseline' frequency after replacement of testosterone in castrated males, (c) the failure of either castration or combined castration and ovariectomy to eliminate genital nuzzling in established pairs, and (d) the exhibition of nuzzling behavior by some of the subordinates in all three experimental colonies beginning several weeks after gonadectomy of both of the reproductives. No cases of lordosis behavior were seen during the approximately 109 h of behavioral observations. This is not surprising, since female mole-rats have an approximately 30-day ovulatory cycle, and lordosis only occurs during a peri-ovulatory period of a few hours. A total of 44 cases of mounting behavior were recorded; all these involved breeding males in colonies or males from isolated pairs, and all occurred when males were either gonad-intact or castrated with testosterone replacement. Thus, in contrast to nuzzling behavior, male sex behavior appeared to be eliminated during androgen deprivation.

Animals↗

Factors affecting post-flight behavior in primary reproductives of the Formosan subterranean termite, Coptotermes formosanus (Isoptera: Rhinotermitidae).

After swarming, reproductive dealates of the Formosan subterranean termite, Coptotermes formosanus, run together in tandem. The tandem running is an age-related behavioral activity in C. formosanus. This behavior was not evoked in pre-flight alates less than 6-d-old that were artificially dealated. Female age was more important than male age for evoking tandem behavior. Females and males, older than 35 days, did not exhibit this behavior. Mating status was not important for female and male dealates to form the tandem pairs. The titers of the major tergal gland component, trilinolein, did not decline significantly and remained high, not only in virgin females, but also in mated females for a period of time after swarming. On the other hand, increasing amounts of the female-specific compound trilinolein were detected in male dealates 7, 14, and 42 days after pairing. This suggests that trilinolein in the females might be transferred to the males as a nuptial gift in C. formosanus. In addition, females have structurally different lateral setae that may constitute a morphological factor involved in the tandem behavior in this species. Covering the setae with dimethyl sulfoxide prevented the tandem behavior.

Age Factors↗

Reproductive condition and behavior in relation to plasma levels of gonadal steroids in the spiny damselfish Acanthochromis polyacanthus.

Gonadal condition and plasma levels of gonadal steroids were measured in relation to behavior in the biparental brood-protecting spiny damselfish Acanthochromis polyacanthus. Fish were captured by SCUBA divers from natural populations on Australia's Great Barrier Reef and immediately bled underwater, following diver or video observation of precapture behaviour. In winter (July), most fish were nonterritorial, with a low proportion of mature males, or vitellogenic females present. In spring (November), most fish were territorial with broods of young at varying stages of maturity, and all stages of gonadal development were represented. Territorial males were larger than nonterritorial fish, but territorial fish that had large (older) young (the end of the brooding phase) had lower condition factors than fish at other stages. Males of all gonad stages had a high proportion of spermatozoa in the testis, but this was higher in November than in July. Ovaries of females commonly had several classes of developing follicles present, although fish that were brooding large young had regressed ovaries with a high incidence of atresia. Plasma levels of testosterone (T) and 11-ketotestosterone were elevated in males of advanced gonadal maturity, and also in relation to recent or imminent spawning behaviour, but there were no changes in plasma 17, 20beta-dihydroxy-4-pregnen-3-one (17,20betaP), which was near assay detection limits at all times. Females had elevated T and 17beta-estradiol (E2) in association with vitellogenesis and elevated T in relation to spawning activity in some fish, but as in males, 17,20betaP levels were low and unchanging. Territorial females without young had lower cortisol levels than nonterritorial fish, or females protecting young. The results confirm the importance of elevated androgens to spawning activity in territorial male fish, but not females where endocrine activity is more closely related to stage of ovarian development. Extended brooding appears to inhibit vitellogenesis, perhaps via a stress-related mechanism.

Animals↗

Reproductive health in adolescence.

The health and well-being of adolescents is closely intertwined with their physical, psychological and social development, but this is put at risk by sexual and reproductive health hazards which are increasing in much of the world. Changes in population growth and distribution, the rise of telecommunications, the increase in travel and a decline in the family, as well as a generally earlier start of menarche and later age of marriage are contributing to an increase in unprotected sexual relations before marriage. This, combined with risks from early marriage, result in too early or unwanted pregnancy and childbirth, induced abortion in hazardous circumstances and sexually transmitted diseases, including HIV infection leading to AIDS. With more than half the world's population below the age of 25, and 4 out of 5 young people living in developing countries with inadequate access to prevention and care, there is an urgent need for action. Young women are particularly vulnerable. Mortality and morbidity from early pregnancy whether ending in childbirth or abortion, is much higher for the younger adolescent. Young women, especially those who have less formal education, are more vulnerable to pressures for marriage, or sexual relations before marriage, often with older men. Young people generally lack adequate knowledge about their own development and information on how to get help. Those who could help are rarely trained for working with adolescents, and services which are generally designed for adults or children often deter young people from getting help when they most need it. Policy and legislation relating to sexual and reproductive health issues are often contradictory, and unclear or unenforced.(ABSTRACT TRUNCATED AT 250 WORDS)

Abortion, Induced↗

Prenatal stress alters reproductive responses of rats in behavioral estrus and paced mating of hormone-primed rats.

Adult female offspring of dams exposed to gestational stress (prenatal stress, PNS) may show altered reproductive behavior, exploration in novel environments, and/or social interactions than do their non-PNS counterparts. These behavioral differences may be more readily observed in a seminatural, paced mating paradigm, in which females have greater control of their sexual contacts, than in a standard mating situation. Adult offspring of dams exposed to restraint and lights for 45 min on Gestational Days 14-20 (PNS) were compared with those not subjected to stress (non-PNS, control condition). The motor, reproductive, and sociosexual behaviors of hormone-primed (Experiment 1) or cycling adult offspring in behavioral estrus (Experiment 2) were examined following 20 min of restraint stress under bright lights (postnatal stress). Hormone-primed PNS rats displayed less motor behavior in a novel arena than did non-PNS rats. In a standard mating test, hormone-primed PNS females tended to be more aggressive toward the male than were non-PNS rats. In a seminatural mating situation, hormone-primed PNS females showed increased avoidance behavior, such as longer latencies to the initial intromission, greater return latencies following mounts and intromissions, and more exiting subsequent to mounts and intromissions, than did non-PNS rats. PNS rats in behavioral estrus had decreased incidence and intensity of lordosis, and fewer solicitation behaviors, in both standard or paced mating situations, in which latency to and number of mounts were also increased. Thus, hormone-primed PNS rats exposed to restraint showed more avoidance behaviors in paced mating situations, while cycling PNS rats in behavioral estrus had greater disruption of reproductive responses in standard or paced mating paradigms than did non-PNS control rats.

Animals↗

Male rat behavior, endocrinology and reproductive physiology in a mixed-sex, socially stressful colony.

Previous research suggests that social stress in the absence of females disrupts male reproductive functioning. The presence of females often increases intermale aggression and, presumably, the probability that fighting----social stress----hyperadrenalism----reproductive disruption. Colony membership of male rats was manipulated in the present research to provoke high or low aggression and the consequent environments that were characterized as high or low socially stressful. The principle comparisons were between all-male and mixed-sex colonies. Results were that the presence of females predictably increased aggressive behavior in both high and low stress environments, yet the adrenal response was different in the two environments. When females were present, adrenal weights and circulating corticosterone levels of males increased in the low stress setting but decreased in the high stress setting. Males cohabitating with females in both environments, on the other hand, experienced elevated titers of circulating testosterone, increased activity of various androgen-sensitive tissues and greater epididymal sperm reserves. The conclusion is that the profound changes in males with sexual contact can attenuate the stress----reproductive disruption relationship.

Adrenal Glands↗

Social suppression of female reproductive maturation and infanticidal behavior in cooperatively breeding Mongolian gerbils.

In several cooperatively breeding species, reproductively suppressed, nonbreeding females are attracted to infants and routinely provide alloparental care, while breeding females may attack or kill other females' infants. The mechanisms underlying the transition from alloparental to infanticidal behavior are unknown. In this study, we tested the hypothesis that this transition is associated with cessation of reproductive suppression and onset of ovarian activity in the Mongolian gerbil (Meriones unguiculatus), a cooperatively breeding rodent. Young female gerbils were housed with their natal family (FH), with a female pairmate (PH) or singly (SH). When females were either 11-13 or 16-18 weeks of age, we characterized their behavioral responses to an unfamiliar pup, reproductive development, and plasma progesterone, cortisol, and prolactin concentrations. In both age groups, FH females were significantly less likely to attack pups than PH or SH females and in fact never did so. FH females also had smaller ovaries and uteri, less developed scent glands, and lower progesterone levels, and were more likely to be anovulatory than PH or SH females, especially in the 11- to 13-week-old age group. Prolactin concentrations did not differ with reproductive status but were significantly higher in females that did not attack pups than in those that did. We found no other significant associations, however, between reproductive or endocrine measures and behavioral responses to pups. These results suggest that cohabitation with the natal family inhibits both infanticidal behavior and reproductive maturation in young female gerbils but that these two effects may not be causally related.

Analysis of Variance↗

Reproductive functions illustrating direct and indirect effects of genes on behavior.

Effects of gene products on reproductive behavior which are relatively direct include those of the estrogen receptor and progesterone receptor. For example, work with estrogen receptor-deficient (ERKO) female mice has extended previous evidence contributing to the neurochemical analysis of lordosis behavior. On the other hand, sex differences in behavior present a classic example of indirect effects of genes on behavior. Work with ERKO male mice shows the necessity of ER gene expression for normal masculinization of the brain. In particular, behavioral assay results distinguish apparent motivational performance of ERKO males from male mating reflexes: the former is similar to that of wild-type males in important respects, while the latter are deficient in ERKO males. The present paper first reviews a small number of clear genetic contributions to reproductive behaviors, and then reports one experiment pertinent to the interpretation of the behavioral status of ERKO male mice.

Animals↗

Role of the amygdala in the reproductive and aggressive behavior of the lizard, Anolis carolinensis.

Thirteen male green anole lizards were lesioned in the ventromedial nucleus (VMN) of the posterior dorsal ventricular ridge ("amygdala") and/or the paleostriatum (PS) to determine the influence of these structures on assertion and challenge displays addressed to male intruders, or courtship displays and copulatory neckgrip directed toward females. Lesions that affected both VMN and PS reduced or eliminated both challenge and courtship displays as well as the neckgrip, a crucial component in courtship. Subjects with lesions limited to VMN had assertion and challenge left unimpaired but courtship was reduced and neckgrip eliminated in most subjects. A lesion restricted to PS caused a significant deficit in challenge while other measures were unaffected. These data indicate that the VMN is involved in reproductive function in the green anole lizard.

Aggression↗

Light and electron microscopic observations of feeding behavior, nutrition, and reproduction in laboratory cultures of Thalassicolla nucleata.

T. nucleata is a skeletonless, single-celled radiolarian commonly found in ocean surface water. Specimens were cultured in the laboratory in a seawater--antibiotic medium. Feeding experiments show that T. nucleata is omnivorous with preference for crustacea, colorless flagellates, and occasional diatoms. Pigmented algae bearing organic thecae are occasionally consumed, but Dunaliella sp. is rejected. Rhizopodial activity during algal predation is compared to activity during crustacean predation, and evidence for differentiation of function among rhizopodia is presented. Electron microscopic observations and evidence from cytochalasin B treatment support the hypothesis that microfilaments mediate organized rhizopodial streaming. Reproduction is by isospores which resemble those in other spumellarian Radiolaria. Survival time in laboratory culture indicates that T. nucleata has a life span of at least 3 weeks. These findings are discussed in relation to the unique requirements imposed by a pelagic habitat on survival of a single-celled floating organism.

Animals↗