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PubMed2006

Eliminating cardiac contamination from myoelectric control signals developed by targeted muscle reinnervation.

The electrocardiogram (ECG) artifact is a major noise contaminating the myoelectric control signals when using shoulder disarticulation prosthesis. This is an even more significant problem with targeted muscle reinnervation to develop additional myoelectric sites for improved prosthesis control in a bilateral amputee at shoulder disarticulation level. This study aims at removal of ECG artifacts from the myoelectric prosthesis control signals produced from targeted muscle reinnervation. Three ECG artifact removal methods based on template subtracting, wavelet thresholding and adaptive filtering were investigated, respectively. Surface EMG signals were recorded from the reinnervated pectoralis muscles of the amputee. As a key parameter for clinical myoelectric prosthesis control, the amplitude measurement of the signal was used as a performance indicator to evaluate the proposed methods. The feasibility of the different methods for clinical application was also investigated with consideration of the clinical speed requirements and memory limitations of commercial prosthesis controllers.

Adult↗
PubMed1991

Neuromuscular transmission to identified primary and secondary myotubes: a reevaluation of polyneuronal innervation patterns in rat embryos.

The early morphogenesis of rat skeletal muscle is a biphasic process involving two sequentially generated populations of myotubes. A small population of primary myotubes appears early and is followed by a much larger population of secondary myotubes which appear progressively over a number of days. All previously published electrophysiological studies of developing muscle have failed to appreciate the relevance of biphasic myotube production. Here we reevaluate the status of early motor innervation, taking into account the wide range of sizes and levels of maturity within the two myotube populations. Evoked end-plate potentials (EPPs) were recorded from fibers of E17-20 rat sternocostalis muscles. Impaled fibers were then marked by ejection of HRP from the recording pipet, enabling ultrastructural identification of fibers from which recordings had been made. The average number of synaptic inputs per fiber increased to a peak at E19, and the rate of rise of the EPPs increased with age. The majority of impaled fibers (76%) were subsequently found to be primary myotubes, even at ages when secondary myotubes formed the majority of fibers in the muscle. Electrophysiological studies during early stages of secondary myotube development therefore sample largely from the more mature primary fibers and probably give the wrong impression of the extent and degree of polyneuronal innervation and of synaptic rearrangement within the muscle. In addition, the results show that very young secondary myotubes are distinguished by EPPs of longer latency, slower rate of rise, and smaller size than those of other types of myotubes. These results suggest that young secondary myotubes are predominantly activated by EPPs which originate in adjoining primary myotubes and propagate electronically to the secondary myotube. We propose a new model of early synaptic rearrangement which accommodates the biphasic nature of muscle development. We also suggest that secondary myotubes do not require direct neural input for the initiation of their development.

Animals↗
PubMed1967

The fine structure of embryonic chick skeletal muscle cells differentiated in vitro.

Dissociated myoblasts from 12-day chick embryos were cultured in monolayer, and the differentiation of skeletal muscle cells was studied by electron microscopy. The results have revealed a striking ultrastructural similarity between the in vivo and the in vitro developing muscle, particularly with respect to the myofibrils and sarcoplasmic reticulum. This study demonstrates that all the characteristic organelles of mature skeletal muscle can develop in vitro in the absence of nerves.

Animals↗
PubMedDate not supplied

MyoD and the transcriptional control of myogenesis.

The basic helix-loop-helix myogenic regulatory factors MyoD, Myf5, myogenin and MRF4 have critical roles in skeletal muscle development. Together with the Mef2 proteins and E proteins, these transcription factors are responsible for coordinating muscle-specific gene expression in the developing embryo. This review highlights recent studies regarding the molecular mechanisms by which the muscle-specific myogenic bHLH proteins interact with other regulatory factors to coordinate gene expression in a controlled and ordered manner.

Animals↗
PubMed2004

Progress of cell proliferation in striated muscle tissues during development of the mouse tongue.

The developmental stages of and places for the proliferation of tongue muscle cells have not yet been determined. To determine the stages of and places for proliferation between embryonic day (E) 9 and birth, we analyzed the expression of cyclin D1 mRNA and the immunolocalization for proliferating cell nuclear antigen (PCNA). The ratio of PCNA-positive nuclei to total nuclei (PCNA-labeling index) was obtained in the anterior, middle, and posterior regions. Cyclin D1 mRNA was highly expressed between E11 and E13, but decreased thereafter until birth. The distribution of PCNA-positive cell nuclei was consistent with that of myogenic cells in the occipital somites at E9. The PCNA-labeling index was highest at E11, then decreased until birth without a significant difference among the 3 regions. These findings suggest that some tongue muscle progenitor cells begin proliferation in the occipital somites at E9, and that the proliferation in the whole tongue region occurred most actively between E11 and E13, then decreased until birth without regional differences.

Animals↗
PubMed1982

Development of contractile properties in avian embryonic skeletal muscle.

The development of the twitch and tetanic responses of the embryonic chick posterior latissimus dorsi muscle has been studied during the last week in ovo. Normalized twitch and tetanic forces increased 3- and 12-fold, respectively, during this period. The changes in the kinetics of the twitch and tetanic responses differed during this developmental period. The time to peak twitch force progressively decreased. The decrease in time to half-peak tetanic force and the increase in the time differential of force production of the tetanic response did not continue after day 18. A prolonged tonic contractile component was described for both the twitch and tetanic responses, particularly in muscles from the younger embryos (days 14-18). A large decrease in the time to one-half relaxation of the twitch response also takes place during the final week in ovo. This detailed description of the development of the contractile properties provides a model system of fast-twitch muscle development in which neurogenic and myogenic components of muscular differentiation can be studied from several approaches.

Animals↗
PubMed2001

Sequential expression of three receptor subtypes for extracellular ATP in developing rat skeletal muscle.

In this study, we investigated the expression of the P2X receptor subtypes (P2X(1-7)) during the development of skeletal muscle and in relation to acetylcholine receptors in the rat embryo and pup. By using immunohistochemistry, we showed that three receptor subtypes, P2X(2), P2X(5), and P2X(6), were expressed in developing skeletal muscle. The timing and pattern of receptor expression seemed to be precisely regulated. P2X(2), P2X(5), and P2X(6) were expressed in a sequential manner, which was consistent for all regional muscles tested (intercostal, paravertebral, and lower limb): P2X(5) expression appeared first (E15-E18) followed by P2X(6) (E16-E18), and finally P2X(2) (E18-adult). At no developmental stage did we observe colocalization of P2X(2) and acetylcholine receptors. In the case of P2X(2) and P2X(6), immunoreactivity was found to be widespread, immunopositive cells being apparent throughout the muscle. However, staining for P2X(5), both at the beginning and end of expression, was restricted to regions of muscle close to the myotendinous junctions. Because the timing of receptor expression is closely related to key events in skeletal muscle development, notably the generation of secondary myotubes and the redistribution of acetylcholine receptors, it is possible that ATP-signaling by means of P2X receptors could be involved in these processes.

Adenosine Triphosphate↗
PubMed1982

Incorporation of donor muscle precursor cells into an area of muscle regeneration in the host mouse.

Normal muscle precursor cells, prepared by the enzymatic disaggregation of neonatal mouse muscle, were implanted into an area of regenerating muscle in a genetically different inbred strain. This was done in an attempt to determine, first, whether donor muscle precursor cells prepared in this way would fuse with the developing muscle fibres of the host; and second, whether in the "mosaic" muscle fibres thus formed donor as well as host genes were expressed. As markers of the host and donor genes we used the allelic isoenzyme variants of glucose-6-phosphate isomerase (GPI). In 43 out of 60 grafts we detected the presence of a "hybrid" isoenzyme intermediate between host and donor types. This "hybrid" indicated that donor muscle precursor cells had fused with regenerating host muscle cells, and had expressed their GPI genes within the resulting mosaic muscle fibres. We have developed this technique with a view to inserting normal genes into genetically abnormal myopathic muscle.

Animals↗
PubMed2005

Proteolytic processing of myostatin is auto-regulated during myogenesis.

Myostatin, a potent negative regulator of myogenesis, is proteolytically processed by furin proteases into active mature myostatin before secretion from myoblasts. Here, we show that mature myostatin auto-regulates its processing during myogenesis. In a cell culture model of myogenesis, Northern blot analysis revealed no appreciable change in myostatin mRNA levels between proliferating myoblasts and differentiated myotubes. However, Western blot analysis confirmed a relative reduction in myostatin processing and secretion by differentiated myotubes as compared to proliferating myoblasts. Furthermore, in vivo results demonstrate a lower level of myostatin processing during fetal muscle development when compared to postnatal adult muscle. Consequently, high levels of circulatory mature myostatin were detected in postnatal serum, while fetal circulatory myostatin levels were undetectable. Since Furin proteases are important for proteolytically processing members of the TGF-beta superfamily, we therefore investigated the ability of myostatin to control the transcription of furin and auto-regulate the extent of its processing. Transfection experiments indicate that mature myostatin indeed regulates furin protease promoter activity. Based on these results, we propose a mechanism whereby myostatin negatively regulates its proteolytic processing during fetal development, ultimately facilitating the differentiation of myoblasts by controlling both furin protease gene expression and subsequent active concentrations of mature myostatin peptide.

Animals↗
PubMed1999

Influence of growth factors on poultry myogenic satellite cells.

Skeletal muscle development in avian and mammalian embryos depends on the proliferation, differentiation, and fusion of embryonic myoblasts. During the late fetal period and following birth or hatching, myogenic satellite cells are responsible for this developmental function. Satellite cells, which are found adjacent to existing skeletal muscle fibers fuse with these fibers and their nuclei direct the synthesis of new protein and function in the maturation of muscle. These events are controlled by specific growth factors that are produced locally by satellite cells and other cells in the muscle. Progress in our understanding of the early events in myogenesis has been made possible by the development of satellite cell cultures and media formulations that allow the assessment of the role of growth factors in skeletal muscle growth and development. Because of the key role that satellite cells play in skeletal muscle growth, development, and regeneration, many scientists in both the agricultural and medical communities have focused their research on understanding the physiology of this cell. From an agricultural perspective, a better understanding of the mechanisms regulating satellite cell activity may lead to procedures to increase the deposition and efficiency of lean muscle (meat) accretion and, perhaps, improve the nutrient composition of meat products.

Animals↗
PubMed2000

Maturational differences between vascular and bladder smooth muscle during ovine development.

Maturation rates of vascular and visceral smooth muscle (SM) during ovine development were compared by quantifying contractile protein, myosin heavy chain (MHC) isoform contents, and contractile properties of aortas and bladders from female fetal (n = 19) and postnatal (n = 21) sheep. Actin, myosin, and protein contents rose progressively throughout development in both tissues (P </= 0.003); however, expression patterns differed. During the last trimester, i. e., 101-130 days (term approximately 145 days), bladder actin and MHC contents were approximately twofold greater (P < 0.04) than those in the aorta. Although the fractional content of 204-kDa SM1 MHC in the bladder decreased from 74 +/- 3% at midgestation to 48 +/- 2% 3 mo postnatal, the aorta exhibited an increase from 30 +/- 2% to 65 +/- 2%. Bladder MHC (MHC-B) migrating at 200 kDa contained only SM2 throughout development. In contrast, 200-kDa MHC in the aorta was predominantly nonmuscle MHC-B at midgestation, which was gradually replaced by SM2 as development progressed. Along with its early expression of SM2, bladder muscle obtained maximal stress generating capacity (1.7 x 10(5) N/m(2)) by term gestation, whereas the aorta exhibited no contractions until after birth. We conclude that whereas aortic SM maturation is delayed until after birth, bladder SM matures biochemically and functionally during prenatal development, thus supporting early requirements for micturition.

Actins↗
PubMed1987

Diversification of the myofibrillar M-band in rat skeletal muscle during postnatal development.

The fine structure of the M-band in soleus (SOL) and extensor digitorum longus (EDL) muscles in newborn and four-week-old rats was studied using electron-microscopic techniques. In newborn rats, all myotubes and fibres in both muscles had an identical myofibrillar appearance. A five-line M-band pattern was seen in longitudinal sections and distinct M-bridges in cross-sections. The Z-discs were of medium width. On the other hand, in four-week-old rats, different muscle fibre types were observed on the basis of their myofibrillar pattern. In SOL two fibre types were distinguished in longitudinal sections. One had a four-line M-band pattern and very broad Z-discs, whereas the other type had five lines in the M-band and broad Z-discs. In EDL, three different myofibrillar patterns were observed. The M-bands were composed of three, four or five lines. Fibres had either thin, broad or medium Z-disc widths, respectively. In cross-sections of the SOL muscle one group of fibres showed indistinct M-bridges, whereas distinct M-bridges were seen in the other fibres and in all observed EDL muscle fibres. We conclude that initially there seems to be a single intrinsic program for M-band genesis; this program becomes modified upon the induction of functionally differentiated fibres.

Aging↗
PubMed1990

Regulation of satellite cells during skeletal muscle growth and development.

Satellite cells are myogenic cells attributed with the role of postnatal growth and regeneration in skeletal muscle. Following proliferation and subsequent differentiation, these cells will fuse with one another or with the adjacent muscle fiber, thereby increasing myonuclei numbers for fiber growth and repair. The potential factors which could regulate this process are many, including exercise, trauma, passive stretch, innervation, and soluble growth factors. Three classes of growth factors in particular (fibroblast growth factor, insulin-like growth factor, and transforming growth factor-beta) have been studied extensively with respect to their effects on satellite cell proliferation and differentiation in culture. Fibroblast growth factor has been shown to stimulate proliferation but depress differentiation. Insulin-like growth factor stimulates both proliferation and differentiation, although the latter to a much greater degree. Transforming growth factor-beta slightly depresses proliferation but inhibits differentiation. When administered in combination, these factors can induce satellite cell activities in culture which mimic those typical of satellite cells found in vivo in growing, regenerating, or healthy mature muscle. Alterations in the concentrations of these growth factors in the muscle environment as well as alterations in the cell's sensitivity or responsiveness to these factors represent potential mechanisms for regulating satellite cell activity in situ.

Animals↗
PubMed1989

A myosin isoform repressed in hypertrophied ALD muscle of the chicken reappears during regeneration following cold injury.

A library of monoclonal antibodies specific for myosin heavy chain (HC) was used to study myosin expression in regenerating fibers. The response to cold injury of slow skeletal ALD muscle previously induced to eliminate SM1 myosin by weight overload was compared to that of its contralateral control. Native gel electrophoresis combined with immunoblotting demonstrated that slow SM1 myosin HC eliminated from hypertrophic muscle reappeared both at the site of active regeneration and unexpectedly, also distal to the site of injury. The regeneration response of hypertrophied muscles was similar to that of the controls. In addition to SM1 myosin HC, ventricular-like and embryonic/fast isoforms were also expressed in both muscles during the early stages of regeneration and disappeared as the muscle fibers matured. These observations demonstrate that regenerating slow muscle fibers reexpress myosins' characteristic of developing muscle irrespective of the myosin phenotype prior to injury. The reappearance of repressed myosin HC in the hypertrophied ALD muscle is consistent with the presence of newly differentiated myonuclei.

Animals↗
PubMed2006

The effects of work experience, lift frequency and exposure duration on low back muscle oxygenation.

BACKGROUND: Previous studies have shown changes in low back muscle oxygenation after static muscle contractions or short-term dynamic lifting exertions. The aim of this study was to document the changes in low back muscle oxygenation during prolonged lifting activity over an entire workday as of function of work experience and lift frequency. METHODS: Four novice and six experienced subjects participated in a lifting study in which they lifted load with a given weight at one of five different frequencies (2, 4, 8, 10, 12 lifts/min) for an 8-h period. Oxygen saturation of the left and right erector spinae was measured continuously and non-invasively using near-infrared spectroscopy during each lifting session. FINDINGS: Exposure duration had a statistically significant effect on muscle oxygenation level (P<0.0001). Oxygen saturation in the erector spinae increased during the 8-h lifting period. As lift frequency increased, back muscle oxygenation in experienced subjects also increased. In general, the increase in muscle oxygenation for experienced subjects was less than that for novice subjects. INTERPRETATION: This study suggested that the requirement of oxygen for the low back muscle in a typical industrial lifting job increased over time and experienced workers responded differently from the novice subjects. These findings may provide more insight into the physiological changes of the working muscle and the potential risks of developing muscle injury.

Back↗
PubMed1996

A case of ectopic anus localized on the right gluteus.

A case of a gluteus-localized ectopic anus on the right side is described. A 5-month-old boy had a severe sacral anomaly with atrophy of the right lower extremity and a pes equinus deformity. Transcutaneous muscle stimulation showed a well-developed muscle complex, and parasagittal muscle fibers have been localized obliquely. Although he has severe sacral anomalies, the posterior sagittal anorectoplasty procedure could be performed. This unique case provides a good demonstration of not only the ectopically localized anus but also the ectopically localized muscle complex.

Abnormalities, Multiple↗
PubMed1997

Effects of neurochemically excited group III-IV muscle afferents on motoneuron afterhyperpolarization.

When humans voluntarily and maximally contract a muscle under isometric conditions, the average firing rate of motor units decreases from an initially high value over several tens of seconds. The mechanisms underlying the rate reduction are probably manifold. One mechanism could involve changes in the motoneuron afterhyperpolarization, another reflex effects of group III-IV muscle afferents that are excited during developing muscle fatigue. It appears possible that changes in motoneuron afterhyperpolarization are mediated by these afferent inputs. We therefore studied effects on motoneuron afterhyperpolarization of small-diameter muscle afferents excited by intra-arterially injected metabolites such as bradykinin and serotonin. In decerebrate and mostly spinalized cats, lumbosacral alpha-motoneurons were recorded intracellularly. Current pulses were injected to test for input resistance and elicit action potentials and afterhyperpolarizations. Afterhyperpolarizations were averaged from c. 10 successive stimulus repetitions. Measurements were taken of afterhyperpolarization amplitude, half-width and area; and exponential functions were fitted to the afterhyperpolarization decay phase to determine afterhyperpolarization decay time-constants. In selected cases, the entire afterhyperpolarization trajectory was fitted with a sum of two exponentials to assess more precisely changes in afterhyperpolarization trajectory. Small catheters were inserted into side-branches of the sural artery and the accompanying vein to apply substances like bradykinin, serotonin and KCl to the calf muscles. Concentrations were in the range of those used by other workers. Intra-arterial injection of bradykinin and serotonin usually decreased blood pressure, which may at times have affected mean motoneuron membrane potentials. Afterhyperpolarization amplitude usually changed with membrane potential in a way expected from ensuing changes in driving potential. Whenever excitation of group III-IV muscle afferents caused moderate to strong increases in motoneuron synaptic noise, afterhyperpolarization amplitudes were reduced, usually in parallel to decreases in input resistance. Afterhyperpolarization half-widths were mostly unaffected, but occasionally decreased. There was a significant trend for afterhyperpolarization decay time-constants to increase during increased synaptic noise, this increase being inversely correlated with the reduction in afterhyperpolarization amplitude. The reduction in input resistance was associated with a decrease in the membrane time-constant, which could therefore not account for the prolongation of the afterhyperpolarization decay time-constant. The afterhyperpolarization area decreased, indicating that the reduction of afterhyperpolarization amplitude outweighed the prolongation of afterhyperpolarization decay time-constant. During a prolonged fatiguing muscle contraction group III-IV afferents become increasingly excited, produce augmenting synaptic inputs in motoneurons, and will change afterhyperpolarization properties. On average, these changes per se tend to diminish the effect of afterhyperpolarization on motoneuron discharge.

Animals↗
PubMed1994

Role of 5 alpha-reductase in health and disease.

The mechanism of androgen action varies in different tissues, but in the majority of androgen target tissues either testosterone or 5 alpha-dihydrotestosterone (DHT) binds to a specific androgen receptor to form a complex that can regulate gene expression. Testosterone is metabolized to DHT by the enzyme 5 alpha-reductase. The autosomal recessive genetic disorder of 5 alpha-reductase deficiency has clearly shown that the requirement for DHT formation varies with different tissues. In this syndrome genetic males contain normal male internal structures including testes, but exhibit ambiguous or female external genitalia at birth; at puberty they undergo partial virilization which includes development of a male gender identity even if brought up as females. Their development suggests that testosterone itself is able to stimulate psychosexual behaviour, development of the embryonic wolffian duct, muscle development, voice deepening, spermatogenesis, and axillary and pubic hair growth; DHT seems to be essential for prostate development and growth, the development of the external genitalia and male patterns of facial and body hair growth or male-pattern baldness. How different hormones operate to regulate genes via the same receptor is currently unknown, but appears to involve cell-specific factors. The 5-alpha-reductase enzyme has proved difficult to isolate biochemically, but recently at least two human isoenzymes have been identified using molecular biological methods. All the various 5 alpha-reductase-deficient kindreds have been shown to have mutations in 5 alpha-reductase 2, the predominant form in the prostate. The biological role of 5 alpha-reductase 1 has not yet been ascertained, but at present it cannot be ruled out that some of the actions ascribed to testosterone are indeed in cells producing DHT via this enzyme. The activity of 5 alpha-reductase is also implicated in benign prostatic hypertrophy, hirsutism and possibly male-pattern baldness; recent evidence discounts the role of 5 alpha reductase 2 in sebaceous glands and acne. Specific inhibitors of both enzymes are now available and finasteride, a 5 alpha-reductase 2 inhibitor, has been used successfully in clinical trials of benign prostatic hypertrophy. Knowledge of 5 alpha-reductase is expanding dramatically at the moment with the application of molecular biological methods. The advent of antibodies to the isoenzymes should herald further understanding of their biological and clinical roles.

Animals↗