SEARCH · Search PubMed
Results for “ChIP”
Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.
Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.
Bone chip plastic repair of congenital chest deformities.
Explore the source record for details and available documents.
Potato chip laceration of the throat.
Explore the source record for details and available documents.
Cumulative radiation tallied by tiny chips.
Explore the source record for details and available documents.
Lab-on-a-chip shows promise in defining and diagnosing cancers.
Explore the source record for details and available documents.
Gene chips inch toward the clinic.
Explore the source record for details and available documents.
Breast cancer gene chip study under way: can new technology help predict treatment success?
Explore the source record for details and available documents.
Comparative genomic hybridization: FISH and chips in our future?
Explore the source record for details and available documents.
From gels to chips: "minisequencing" primer extension for analysis of point mutations and single nucleotide polymorphisms.
In the minisequencing primer extension reaction, a DNA polymerase is used specifically to extend a primer that anneals immediately adjacent to the nucleotide position to be analyzed with a single labeled nucleoside triphospate complementary to the nucleotide at the variant site. The reaction allows highly specific detection of point mutations and single nucleotide polymorphisms (SNPs). Because all SNPs can be analyzed with high specificity at the same reaction conditions, minisequencing is a promising reaction principle for multiplex high-throughput genotyping assays. It is also a useful tool for accurate quantitative PCR-based analysis. This review discusses the different approaches, ranging from traditional gel-based formats to multiplex detection on microarrays that have been developed and applied to minisequencing assays.
Liquid chromatography on-chip.
Explore the source record for details and available documents.
An enhanced microfluidic chip coupled to an electrospray Qstar mass spectrometer for protein identification.
The combination of microfabricated fluidic systems (muFAB) and electrospray mass spectrometers (ESI-MS) will provide multiplexed and integrated analytical systems for proteins and other biomolecules. Implementation of this novel approach requires the development of robust and user-friendly muFAB devices. Here, we present new approaches that improve the robustness, user friendliness and performance of muFAB devices coupled to MS. First, we present the development of a convenient mount to connect a muFAB device to the ESI-MS and the incorporation of filters in the reservoirs and exit of the muFAB. This mount facilitates interfacing and significantly reduces the chemical noise observed by the MS. Furthermore, we demonstrate improvements in sample handling and delivery by using either a nonaqueous electrolyte or a cationic coating on the surfaces in the muFAB device and transfer capillary. These improvements are applied to protein analysis by continuous infusion of proteolytic digests.
A free-flow electrophoresis chip device for interfacing capillary isoelectric focusing on-line with electrospray mass spectrometry.
When electrospray ionisation mass spectrometry (ESI-MS) is used on-line with capillary isoelectric focusing (CIEF), the presence of the carrier ampholytes creating the IEF pH gradient is not desirable. With the purpose of removing these ampholytes, we have developed a free-flow electrophoresis (FFE) device and coupled it to CIEF. The different parameters inherent to the resulting CIEF/FFE system were optimised using ultraviolet absorbance (UV) detection. The on-line coupling of this system with ESI-MS was successfully realised for three model proteins (myoglobin, carbonic anhydrase I and beta-lactoglobulin B).
Clinical and histological findings in guided bone regeneration (GBR) around titanium dental implants with autogeneous bone chips using a new resorbable membrane.
The aim of this study was to test the value of a polydioxanon-membrane (PDS(R), Ethicon, Norderstedt, Germany) in combination with autogenous bone as a spacer for guided bone regeneration of denuded implant surfaces in comparison to simple augmentation. Altogether, in 8 Beagle dogs of 12 months, the three mandibular premolars were extracted on both sides and, after an uneventful healing of the extraction sockets in each mandibular site, two seize defined bone defects were produced. The defects were 7 mm long, had a vertical extension of 7 mm, and were 7 mm deep. These also included, therefore, the resection of the lingual cortical bone wall. In the center of all these 32 bone defects, Brânemark fixtures with a diameter of 3 mm and a length of 10 mm were implanted. Every implant was primarily stable due to the fixation in the native bone under the created defects. In each mandible site, one bone defect was filled with autologous bone resulting from the previous defect preparation. Half of the defects were covered with a PDS membrane, and the other half were covered only by the relocation of the mucoperiosteal flap. During the observation period of 90-180 days, continuous clinical and radiological controls were executed. Aside from the observed clinical healing problems in the test sites, such as exposure of the membranes and serious inflammatory reactions, the histological and radiological results of the control group regarding bone regeneration were superior to these of the test group, which were valid also for the subgroups with or without autologous bone fillings.
A Chip-Based Biosensor for the Functional Analysis of Single Ion Channels We thank E. Ermanntraut, L. Giovangrandi, T. Wohland, A. Brecht, M. Köhler, C. Bieri, D. Stamou, and R. Hovius for advice. This work was supported by the Swiss National Science Foundation (Priority Program for Biotechnology) and by an interdepartmental grant of the Swiss Federal Institute of Technology Lausanne (EPFL, Project Microtechnique 96).
Explore the source record for details and available documents.
Fabrication of carbohydrate chips for studying protein-carbohydrate interactions.
Explore the source record for details and available documents.
Proteomics on a chip: promising developments.
The field of proteomics is expanding rapidly due to the completion of the human genome and the realization that genomic information is often insufficient to comprehend cellular mechanisms. This considerable expansion of proteomics towards high-throughput platforms is stressing its current technical capabilities. In recent years, technologies in microfluidic and array technologies have appeared for proteomics. These novel approaches might help solve current technical challenges in proteomics. This review presents a general survey of the recent development in microfluidic and array technologies from a proteomics perspective.
New approaches for fabrication of microfluidic capillary electrophoresis devices with on-chip conductivity detection.
In practice, microfluidic systems are based on the principles of capillary electrophoresis (CE), for a large part due to the simplicity of electroosmotic pumping. In this contribution, a universal conductivity detector is presented that allows detection of charged species down to the microM level. Additionally, powderblasting is presented as a novel technique for direct etching of microfluidic networks. This method allows creation of features down to 50 microm with a total processing time (design to device) of less than one day. The performance of powderblasted devices with integrated conductivity detection is illustrated by the separation of lithium, sodium, and potassium ions and that of fumaric, malic, and citric acid.
High-throughput characterization for organic pollutants in environmental waters using a capillary electrophoresis chip.
To evaluate organic pollution in water, we did preliminarily studies on high-throughput characterization of organic pollution in water using microchip-based capillary electrophoresis (CE) with laseer-induced fluorescence (LIF) detection. The applied voltage was investigated to control the gated valve injection and CE separation for conventional cross type microchips using a self-made personal computer (PC)-based controller as the voltage supply. We obtained high-throughput data for the reproducible separation of fluorescein isothiocyanate (FITC)-labeled river-water samples using a zwitter-ion based buffer solution to avoid adsorption of the labeled sample onto the channel of a microchip made from quartz glass. We used real samples from the Hino River that flows into Lake Biwa, from ten sampling points and obtained several reproducible peaks in different separation patterns for each sample within 2 min. We successfully demonstrated high-throughput characterization of dissolved organic carbon (DOC) in environmental water using the microchip.