Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “SCALDS”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,495 records · Page 83Linked to original sources
PubMed2002

Cross-contamination of carcasses and equipment during pork processing.

AIMS: The cross-contamination events within a commercial pork processing line were examined by a combination of ERIC-PCR DNA fingerprinting of Escherichia coli and plate counts. METHODS AND RESULTS: Sponge sampling of environmental surfaces and carcasses was performed over an 8-h processing period. Prior to the start of processing the scraper and dry polisher blades were found to harbour substantial Enterobacteriaceae and Escherichia coli populations. From plate count data the key cross-contamination site for the transfer of bacteria between carcasses occurred during evisceration. However, DNA fingerprints of representative E. coli isolates identified that genotypes initially present on the scraper/dry polisher became distributed on wet polisher blades, band-saw and butcher's hands despite a singeing step being performed post dry polishing. A high proportion of E. coli on post-eviscerated carcasses could be traced to down-stream (pre-singe) environmental contact surfaces. CONCLUSIONS: DNA fingerprinting has demonstrated that E. coli and potential enteric pathogens can be transferred between pork carcasses throughout the processing line. In this respect scalding and singeing cannot be relied upon to control cross-contamination of enteric bacteria between carcasses. SIGNIFICANCE AND IMPACT OF THE STUDY: Sole reliance on indicator organism counts to identify cross-contamination events as currently advocated is limited.

Aerobiosis↗
PubMed2002

A simple random amplified polymorphic DNA genotyping method for field isolates of Dermatophilus congolensis.

Dermatophilus congolensis is the pathogenic actinomycete that causes dermatophilosis in cattle, lumpy wool in sheep and rain scald in horses. Phenotypic variation between isolates has previously been described, but its genetic basis, extent and importance have not been investigated. Standard DNA extraction methods are not always successful for D. congolensis due to its complex life cycle, one stage of which is encapsulated. Here we describe the development of rapid and reliable DNA extraction and random amplified polymorphic DNA (RAPD) methods that can be used for genotyping D. congolensis field isolates. Our results suggest that genotypic variation between isolates correlates with host species. Several DNA extraction methods and RAPD protocols were compared. An extraction method based on incubation of the bacterium in lysozyme, sodium dodecyl sulphate (SDS) and proteinase K treatments and phenolic extraction yielded high-quality DNA, which was used to optimize RAPD-polymerase chain reaction (PCR) protocols for two random primers. An alternative rapid, non-phenolic extraction method based on proteinase K treatment and thermal shock was selected for routine RAPD typing of isolates. DNA extracted from reference strains from cattle, sheep and horse using either method gave reproducible banding patterns with different DNA batches and different thermal cyclers. The rapid DNA extraction method and RAPD-PCR were applied to 38 D. congolensis field isolates. The band patterns of the field and type isolates correlated with host species but not with geographical location.

Actinomycetales↗
PubMed2001

Child injury morbidity in New Zealand, 1987-1996.

OBJECTIVES: New Zealand is one of a small number of countries that has a national hospital discharge database. The aim of this study was to use these data to provide an epidemiological description of child injury morbidity and to discuss options for prevention. METHODOLOGY: Using national data, all public hospital admissions for the fiscal year 1995/6 were identified and grouped according to cause. This allowed identification of all children (0-14 years) hospitalized for injury in the period 1987-1996. Causes of injury, diagnoses and injury severity were examined in four age groups. RESULTS: Injury was the second leading cause of public hospital admissions. Children were hospitalized with injury at the rate of 1333 per 100 000 person-years. The victims were predominantly male (61%). In all age groups, falls were the leading cause of morbidity. 'Striking events', motor vehicle traffic crashes and poisoning were also major contributors to the child injury toll. The commonest injury diagnosis was a fracture of the upper limb (25%), followed by intracranial injury (18%). CONCLUSIONS: The leading causes of injury morbidity were noted to differ from previously reported leading causes of injury mortality. Review of the data in light of research literature suggests the need for policy changes to make alternatives to motor vehicle transportation safer and to encourage increased use of child restraints in order to reduce road traffic trauma. Legislation requiring child-resistant packaging for all toxic medications and household products is necessary to reduce rates of child poisoning. Further research is required on policies concerning playground equipment heights and surfacing requirements, and on interventions for hot water scalds.

Adolescent↗
PubMed2003

Unintentional residential child injury surveillance in Hong Kong.

OBJECTIVES: To provide an overall pattern of morbidity in unintentional residential childhood injuries (URCI) in Hong Kong. METHODOLOGY: A cross-sectional telephone survey of caregivers of children aged under 16-years and adolescents suffering from URCI and admitted to three selected local Accident and Emergency Departments. RESULTS: Falls, cuts and scalds were the most common external causes of URCI observed, while boys predominated in the sample population. Most of the observed URCI were of moderate to mild severity. Children of new immigrant mothers were more likely to receive first aid immediately after the incidents. Parents were aware of potentially injurious behaviour and intervened on occasion, but most resorted to verbal warnings only. CONCLUSIONS: Prevalence of falls among observed URCI offers evidence in support of the hypothesis that the high population density in Hong Kong plays an integral role in understanding mechanisms of morbidity. Parents show concern about URCI but often lack substantial action that modifies injury risk. Considering the local injury differentials, an active prevention effort such as behavioural intervention and education for parents may be useful.

Accident Prevention↗
PubMed2002

Four case reports of families with esophageal cancer in a high-risk region on the Guangdong Chaoshan coast of China.

Four families with a history of esophageal cancer were studied and their family trees analyzed. All the families had lived in Chaoshan for about 20 generations, speak Chanshan dialect, and generally have a predilection for drinking scalding Gong Fu tea and eating pickled Chinese cabbage. The majority of the esophageal cancer patients of the first generation were diagnosed 40 or 50 years ago after presenting with the typical symptom of dysphagia, whereas patients of the second and third generations were diagnosed mainly by means of radiography and pathology. The ratio of male to female patients was 14:5, which corresponds to that in the general population. The average age at occurrence of esophageal carcinoma in the patients studied was lower than in the general population and had progressively decreased from generation to generation.

Aged↗
PubMed1996

Burns to the genitals and the perineum in children.

OBJECTIVE: To determine the management of perineal and genital burns in children. PATIENTS AND METHODS: Twenty-seven children (aged 6 months to 12 years) suffering from genital or perineal burns between 1981 and 1995 were reviewed. Most burns occurred in boys (70%), were due to scalds (85%) and caused superficial second-degree lesions (63%). The initial treatment consisted of topical antimicrobials or grafting with allografts. RESULTS: Spontaneous healing by conservative therapy, not early excision therapy, occurred in 96% of the patients. Contractures occurred in two patients and were treated with multiple Z-plasties and circumcision. CONCLUSION: The management of perineal and genital burns in children should be conservative.

Burns↗
PubMed1997

Genes for albicidin biosynthesis and resistance span at least 69 kb in the genome of Xanthomonas albilineans.

All Tn5 insertion mutants of Xanthomonas albilineans, the cause of leaf scald disease of sugar cane, which failed to produce albicidin antibiotics failed to cause chlorosis in inoculated sugar cane but remained resistant to albicidin. Southern analysis revealed that mutants deficient in albicidin production carried the transposon on different chromosomal restriction fragments spanning at least 50 kb in the X. albilineans genome, which is larger than any reported cluster of genes involved in the production of a bacterial phytotoxin. Albicidin-resistant cosmid clones from a Tox- Tn5 insertion mutant did not carry the transposon, and the subcloned albicidin resistance gene did not hybridize to any of the restriction fragments carrying Tn5 in the Tox- mutants, indicating that the albicidin biosynthesis and resistance genes are not closely linked in X. albilineans.

Anti-Bacterial Agents↗
PubMed1998

Isolation of Salmonella resistant to hypochlorous acid from a poultry abattoir.

Salmonella is a Gram-negative bacterium that has been implicated in several food-borne poisoning outbreaks. Poultry products are considered to be the major vehicles of food-borne poisoning caused by Salmonella. The resistance of Salmonella, isolated from different stages in a local poultry abattoir, to hypochlorous acid (HOCl) was studied. Isolates cultured in quarter strength Tryptone Soya Broth were treated with 72 ppm HOCl, incubated at 30 degrees C with shaking, and absorbance was measured at 660 nm every 20 min. A number of resistant isolates, which carried on growing following HOCl addition, were obtained. All the isolates from the bleeding stage were sensitive to HOCl, whereas those from the scalding stage were resistant to HOCl. Other stages had a population of resistant and sensitive isolates. It is evident that some Salmonella will grow in the presence of concentrations of HOCl deemed to be inhibitory. Hence, an effective concentration of HOCl must be applied in poultry abattoirs to ensure that all the Salmonella are eradicated.

Animals↗
PubMed1993

Effect of burn injury on corticosteroid-binding globulin levels in plasma and wound fluid.

Corticosteroids exert inhibitory effects on wound healing. They circulate, largely bound to corticosteroid-binding globulin, and the plasma concentrations of this protein determine their bioavailability. The amount of corticosteroid-binding globulin in wounds and the related effects of burn injury are not known. We have therefore measured corticosteroid-binding globulin in serum and wound fluid obtained from subcutaneously implanted sponges, retrieved 1, 3, and 10 days after insertion in rats. The effect of burning was studied by comparing rats that had a small scald burn with sham-burned control rats. In serum, corticosteroid-binding globulin levels were lower in burned rats than in control animals: the difference was 22%, 28%, and 37% for days 1, 3, and 10, respectively (p < 0.05 for each comparison), and values at day 1 were lower than at days 3 and 10 in control rats (p < 0.05) but not in burned rats. In wound fluid, corticosteroid-binding globulin levels were lower in burned rats than in control animals: the difference was 23%, 24%, and 34% for days 1, 3, and 10, respectively (p < 0.01 for all comparisons), and the values were significantly higher (p < 0.05) at day 1 when compared with values at day 10 in both groups. We therefore conclude that a small burn injury has significant effects on levels of corticosteroid-binding globulin on serum and wound fluid corticosteroid-binding globulin. The decreased concentration of wound fluid corticosteroid-binding globulin at day 10 versus day 1, with a concomitant increase in serum corticosteroid-binding globulin, suggests an accelerated degradation of the protein within the wound; this phenomenon is exaggerated by the burn injury. This is supported by Western blot analysis, which revealed the appearance of a small polypeptide that reacts with an antiserum against rat corticosteroid-binding globulin in wound fluid at day 10.

Journal Article↗
PubMedDate not supplied

Elevated monocyte chemoattractant protein-1 levels following thermal injury precede monocyte recruitment to the wound site and are controlled, in part, by tumor necrosis factor-alpha.

In previous studies, mice given a full-thickness scald injury had an influx of neutrophils into the skin that followed a local increase in a neutrophil chemoattractant. Because macrophages are known to infiltrate the wound area after neutrophils and are essential for normal wound repair, studies were designed to characterize the time course of macrophage accumulation in the wound and to identify the factor(s) responsible for this influx. A macrophage infiltrate into the wound was observed at 4 days post-injury and persisted through at least 10 days. This influx was preceded by an initial fourfold increase in dermal monocyte chemoattractant protein-1 levels at 24 hours post-injury (p < 0.05). This elevation in monocyte chemoattractant protein-1 was enhanced at 4 and 10 days postburn resulting in a sixfold increase over baseline (p < 0.01). Levels of tumor necrosis factor-alpha, a proinflammatory cytokine known to induce chemokine production, were elevated at 90 minutes after injury in burn- versus sham-injured groups (p < 0.05). Furthermore, administration of tumor necrosis factor-alpha neutralizing antibody in vivo reduced the dermal levels of monocyte chemoattractant protein-1 seen at 10 days postburn by 57% (p < 0.01); however, macrophage accumulation was not altered. Thus, elevated systemic TNF-alpha levels may influence the local chemokine milieu following burn injury.

Animals↗
PubMedDate not supplied

Vulvar disease in children: a clinical audit of 130 cases.

We evaluated 130 prepubertal girls presenting with a vulvar complaint to determine the spectrum and frequency of conditions seen in this age group. Of the patients, 41 (33%) had atopic or irritant dermatitis, 23 (18%) had lichen sclerosus, 21 (17%) had psoriasis, 15 (12%) had vulvar lesions, most often hemangiomas and nevi, and 13 (10%) had streptococcal vulvovaginitis. Diagnoses less frequently seen were staphylococcal folliculitis (four patients), labial fusion (three patients), genital warts (two patients), molluscum contagiosum of the vulva only (one patient), vulvar bullous pemphigoid (two patients), scabies nodules (one patient), erythema annulare centrifugum (one patient), tinea (two patients), and vitiligo (one patient). We also encountered vulvar presentations of systemic diseases (varicella, staphylococcal scalded skin syndrome, and Henoch-Schönlein purpura, all one patient each). We did not see candidal vulvovaginitis in this age group nor did we encounter bacterial infection with pathogens other than Staphylococcus aureus and S. pyogenes.

Child↗
PubMed2001

Effectiveness of beta-glucan collagen for treatment of partial-thickness burns in children.

BACKGROUND/PURPOSE: Beta glucan collagen matrix (BGC), which combines the carbohydrate beta-glucan with collagen, has been used as a temporary coverage for adult partial thickness burns with reported good results. Observed advantages of BGC coverage include reduction of pain, improved healing, and better scar appearance. Potentially even more important in children is the elimination of painful daily dressing changes to the burned epithelial surface, as well as decreased fluid loss. This report details the authors' 2-year experience with BGC in a pediatric burn center. METHODS: Retrospective chart review of 225 consecutive pediatric patients treated at our institution between 1997 and 1999 identified 43 patients (19%) with suspected partial thickness burns treated with BGC as the primary wound dressing. BGC was applied to a debrided burn wound and secured with steri-strips, kerlix, and an ace wrap. After 24 hours, adherence of the BGC was confirmed and then left open to air. RESULTS: The most common cause of burn injury was scald (61%), followed by flame (37%), and contact (2%). The average age of patients was 5.5 years (range, 6 weeks to 16 years) and mean percent total body surface area burned was 9.3% (1% to 35%). Thirty-four patients (79%) had the BGC remain intact while the wound healed underneath, with excellent cosmetic results, minimal analgesic requirements, and no need for repetitive dressing changes. Nine patients (21%) had the BGC removed before wound healing: 6 patients lost the BGC because of progression of the burn to full thickness, 2 had BGC nonadherence over a joint, and 1 had an unexplained nonadherence. CONCLUSIONS: Partial-thickness burns in children can be effectively treated with BGC with good results, even in infants and toddlers. BGC markedly simplifies wound care for the patient and family and seems to significantly decrease postinjury pain.

Bandages↗
PubMed2001

The use of a bioactive skin substitute decreases length of stay for pediatric burn patients.

BACKGROUND: To optimize burn care for children, the authors introduced a protocol incorporating the use of a bioactive skin substitute, TransCyte (Advanced Tissue Sciences, La Jolla, CA). This study was designed to determine whether this management plan was safe, efficacious, and decreased hospital inpatient length of stay (LOS) compared with conventional burn management in children. METHODS: All pediatric burns greater than 7% total body surface area (TBSA) that occurred after October 1999 underwent wound closure with TransCyte (n = 20). These cases were compared with the previous 20 consecutive burn cases greater than 7% TBSA that received standard therapy. Standard therapy consisted of application of antimicrobial ointments and hydrodebridement. The following information was obtained: burn mechanism, age, size of burn, requirement of autograft, and LOS. Data were analyzed using the student's t test. RESULTS: Data for age, percent TBSA burn and LOS are reported as means +/- SEM. The children who received standard therapy were 2.99 +/- 0.7 years compared with those receiving TransCyte were 3.1 +/- 0.8 years. There was no difference between the treatment groups with regard to percent TBSA burn: standard therapy, 14.3 +/- 1.4% TBSA versus TransCyte, 12.7 +/- 1.3% TBSA. There was no difference in the type of burns in each group, the majority were liquid scald type, 70% in the standard therapy group versus 90% in the TransCyte group. Only 1 child in the TransCyte group required autografting (5%) compared with 7 children in the standard therapy group (35%). Children treated with TransCyte had a statistically 6 significant decreaed LOS compared with those receiving standard therapy, 5.9 +/- 0.9 days versus 13.8 +/- 2.2 days, respectively (P =.002). CONCLUSIONS: This is the first study using TransCyte in children. The authors found that this protocol of burn care was safe, effective, and significantly reduced the LOS. This new approach to pediatric burn care is effective and improves the quality of care for children with burns.

Burn Units↗
PubMedDate not supplied

Experimental evaluation of urinary bladder marsupialization in male goats.

OBJECTIVE: To evaluate the outcome of urinary bladder marsupialization in male goats. STUDY DESIGN: Prospective, experimental study. ANIMALS: Six healthy mixed-breed male goats. METHODS: After experimentally induced urethral obstruction, 6 male goats had urinary bladder marsupialization. Renal ultrasonography, complete blood count, and serum biochemical analysis were evaluated preoperatively (day 0), at 7 postoperative days, and then at 30-day intervals until 180 days. Stomal diameter was recorded immediately postoperatively and at each postoperative interval. Necropsy examination was performed on day 180 or when stomal stricture or death occurred. RESULTS: Stomal stricture occurred in 1 goat at 120 days. Another goat was found dead at 150 days; severe, suppurative cystitis was identified on necropsy. All goats had mild urine scald dermatitis. Serum biochemical values remained within normal limits, but significant decreases in white blood cell count, serum creatinine concentration, and stomal diameter occurred. At necropsy, all bladders were tubular in shape. Histological evidence of chronic suppurative cystitis and chronic, mild lymphoplasmacytic pyelitis occurred in all goats. Bacterial culture of renal tissue yielded growth in 3 goats, and bladder mucosal swabs yielded bacterial growth in all goats. CONCLUSIONS: Although clinical signs of ascending urinary tract infection were not observed in goats with patent stomata, urinary bladder marsupialization may result in ascending urinary tract inflammation or infection. CLINICAL RELEVANCE: Based on our results, urinary bladder marsupialization should be recommended with caution as the primary method for management of urinary tract obstruction in clinical cases.

Animals↗
PubMed2001

[Accidents in preschool children in the Boblingen district--accident causes and risk groups].

The characteristics of childhood accidents including their socio-demographic distribution were analysed using a cross-sectional design. In December 1999, questionnaires were sent to 50 kindergartens in the 'Landkreis Böblingen' (Southern Germany). 2,963 children went to these kindergartens, and the parents of the children were asked to fill in the questionnaire which was then analysed anonymously by the local health authority of Böblingen. The parents of 1,866 children sent back the questionnaire; the response rate was 72.0% for children with German nationality and 31.6% for the other children. On the average the parents reported 26 'accidents leading to a physician contact' per 100 child-life-years. Four indicators were used for characterising the socio-economic status of the parents (per capita income, occupational status of the father, school education of the mother, summary index), and three groups (low, medium and high socio-economic status) were constructed with each including about 1/3 of the children. Children from the low status group had about 13% fewer accidents than children from the high status group; for severe accidents, however, a contrary association was found (OR of the low as compared with the high status group: 4.1; Conf. Inter.: 1.12-14.96). The children with another nationality than German had severe accidents more often than German children (OR: 4.63; Conf. Inter.: 2.24-9.52). Boys had 33% more accidents than girls. About 3/4 of all accidents took place at home or in its direct environment. Concerning the age distribution, a peak was seen at age 2. The following accidents occurred particularly often: falling from stairs (10.2% of the accidents), falling from a playground equipment (9.5%), falling from a bicycle (8.9%), falling from a high bed (6.0%), stabs or cuts (4.9%), burns or scalds (4.4%), falling from a high chair (3.9%), falling from a nursery table (3.2%). The results are used by a regional working group which is asked to develop a strategy aimed at reducing accidents of pre-school children in the 'Landkreis Böblingen' until 2006 by 10%.

Accidental Falls↗
PubMed2002

[Accidents of toddlers and youngsters].

The Public Health Department in Biberach an der Riss developed a questionnaire to investigate the incidence of accidents in children under school-starting age (6 years). This questionnaire was presented to the parents of more than 2,300 prospective first-graders from the town and rural district on the occasion of the pre-school medical examination 2000. As this examination is mandatory for all children starting school, and as the questions were answered by all the parents with very few exceptions (language reasons), a complete survey can be assumed. The investigation confirmed the results of last year: The incidence of children who suffered an accident requiring medical attention before reaching school age is approximately 33 %; boys are predominantly involved. The scene of accidents also changes with increasing age from living quarters to outside areas. The most frequent type of accidents are, of course, falls, resulting especially in injuries to the head and face. Scalds and burns, in particular at the age of 2, occur more frequently in the Biberach district than described in other up-to-date investigations in Germany. For this reason efforts have to be made to reduce this number over the next years. About 11 % of accidents occur in the streets or involve traffic, a result which is also higher in comparison to other investigations. According to the statement of parents, more than two-thirds of accidents are caused by the children themselves, including babies and toddlers. At the time of the accident 40 % of the children were without parental control, and 20 % completely alone.A great number of the accidents could certainly have been prevented. That is why the results of the study should be made available to all those responsible for the care and wellbeing of this age group. The last section of the paper deals with the most urgent needs of action to be implemented in the long run for the sake of the health of our children.

Accidents↗
PubMed2004

Conscious sedation: Off-label use of rectal S(+)-ketamine and midazolam for wound dressing changes in paediatric heat injuries.

BACKGROUND: Wound dressing changes after heat injuries expose the patient to repeated painful and frightening procedures in short intervals. Safe, adequate, and easily administered analgesia and sedation are required. The goal of this study was to evaluate the off-label use of rectally administered S(+)-ketamine and Midazolam by paediatric surgeons during repeated outpatient dressing changes for paediatric burns and scalding. PATIENTS AND METHODS: A total of 47 dressing changes of 30 children with I - IIa degrees burns were evaluated. Vital signs, side-effects, complications, anxiolysis, and analgesia were recorded during the procedure and for the following two hours. Patients were assessed by a discharge scoring system and an age-appropriate pain scoring system at regular intervals. Before discharge, parents were interviewed on their level of satisfaction with the protocol. RESULTS: Adequate sedation and analgesia was achieved in 44 procedures (94 %). No complications and, in particular, no compromise of breathing, ventilatory, and cardiovascular functions were recorded. The discharge scoring system indicated a return to baseline function 30 minutes after the procedure in all patients. The parents were generally very satisfied with the protocol. All children old enough to be questioned were found to have an anterograde amnesia for the duration of the procedure. CONCLUSION: Conscious sedation with rectally applied S(+)-ketamine and Midazolam allows safe and painless dressing changes after heat injuries in children.

Adjuvants, Anesthesia↗