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At least 1,495 records · Page 83Linked to original sources
PubMed1983

[Generation of membrane potential by aerobic bacteria Micrococcus lysodeikticus. Correlation between coupled and uncoupled respiration].

The effects of cyanide and nonylhydroxyquinoline-N-oxide on membrane potential generation and oxidase activities in intact cells and membrane particles of M. lysodeikticus were studied. Low concentrations of the inhibitors interacting with the components of one branch of a branched respiratory chain of M. lysodeikticus strongly inhibit the membrane potential generation and only slightly reduce the total respiration rate. It was assumed that the generation of delta psi takes place in the b-c locus of the respiratory chain branch terminated with cytochrome oxidase; over 90% of total O2 uptake provides for the uncoupled respiration via cytochrome omicron. Inside the b-c locus two sites responsible for delta psi generation were singled out; these sites differ in their sensitivities towards nonylhydroxyquinoline-N-oxide. Based on the data obtained and the effect of "extra-reduction" of cytochrome b566 a hypothetical model of the respiratory chain of M. lysodeikticus was developed, which includes the branching of the electron flux to two terminal pathways and redox equivalent transport cyclization via the menaquinone cycle. The physiological role of uncoupled respiration through cytochrome omicron is discussed.

Aerobiosis↗
PubMed1983

[Study of a free radical compound from the cells of the bacteria Micrococcus lysodeikticus].

Some physico-chemical properties of a compound previously detected in M. lysodeikticus cells were studied. This compound is hydrophilic, has hydrophilic, has a low molecular weight and does not contain The redox potentials of the free radical formation in the cytoplasm and in purified preparations were determined. A hypothetical reaction scheme is proposed.

Electron Spin Resonance Spectroscopy↗
PubMed1978

Repair of the double-strand breaks produced by 125I disintegrations in the DNA of Micrococcus radiodurans.

Wild-type M. radiodurans and two radiosensitive mutants were used to study the lethal effects of 125I disintegrations in their DNA. The relative sensitivities of these three strains to inactivation by gamma-radiation were reflected in their relative sensitivities to inactivation by 125I decay. The number of double-strand (ds) breaks in the DNA appeared to be similar at levels of gamma-radiation and of 125I decay that reduced survival to 10%. All three strains of M. radiodurans rapidly repaired ds breaks produced in their DNA by either gamma-radiation or 125I disintegrations. If one ds break per is a lethal event [Kirsch et al., 1975], cells of the three strains tested would die only when they had left unrepaired one ds break out of an initial 45,600 or 1800 ds breaks per single cell.

Cell Survival↗
PubMed1984

[Specific binding of fluorescein bimercuric acetate by histidine decarboxylase from Micrococcus].

The SH-groups of histidine decarboxylase were modified by means of fluorescent probe--fluorescein bimercuric acetate (FMA). Native histidine decarboxylase bound 3 molecules of FMA with complete inhibition of the enzymatic activity. Binding of FMA with the enzyme was accompanied by distinct alteration in absorption and fluorescence spectra. The FMA was also used in studies of SH-containing peptides of histidine decarboxylase; acid SH-peptides obtained after tryptic hydrolysis appears to contain the cysteine of the enzyme active site.

Binding, Competitive↗