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Reproductive performance of the mite Brevipalpus phoenicis (Geijskes, 1939) on citrus and coffee, using life table parameters.

The flat-mite Brevipalpus phoenicis (Geijskes, 1939) (Acari: Tenuipalpidae) is considered important in citrus (Citrus spp.) and coffee plants (Coffea spp.) in Brazil, and is known as the leprosis and coffee ring spot mite, as being a vector of the Citrus Leprosis Rhabdovirus - CitLV and Coffee Ring Spot Virus - CoRSV. The objective of this work is to find out about the reproductive success of B. phoenicis on citric fruits and coffee leaves by fertility life table parameters and its biology. The experiments were carried out in laboratory conditions at 25 +/- 2 degrees C, 70 +/- 10% of relative humidity and 14 h of photophase. The lengths of embryonic and post-embryonic periods were different due to the host where the mite was reared. B. phoenicis showed better development and higher survival and fecundity in citric fruits than coffee leaves. The intrinsic rate of the population increase (r(m)) was 0.128 and 0.090 - females/female/day on citric fruits and coffee leaves, respectively. The citric fruits were more appropriate for the development of B. phoenicis than coffee leaves.

Animals↗

Comparative laboratory toxicity of neem pesticides to honey bees (Hymenoptera: Apidae), their mite parasites Varroa jacobsoni (Acari: Varroidae) and Acarapis woodi (Acari: Tarsonemidae), and brood pathogens Paenibacillus larvae and Ascophaera apis.

Laboratory bioassays were conducted to evaluate neem oil and neem extract for the management of key honey bee (Apis mellifera L.) pests. Neem pesticides inhibited the growth of Paenibacillus larvae (Ash, Priest & Collins) in vitro but had no effect on the growth of Ascophaera apis (Olive & Spiltoir). Azadirachtin-rich extract (neem-aza) was 10 times more potent than crude neem oil (neem oil) against P. larvae suggesting that azadirachtin is a main antibiotic component in neem. Neem-aza, however, was ineffective at controlling the honey bee mite parasites Varroa jacobsoni (Ouduemans) and Acarapis woodi (Rennie). Honey bees also were deterred from feeding on sucrose syrup containing > 0.01 mg/ml of neem-aza. However, neem oil applied topically to infested bees in the laboratory proved highly effective against both mite species. Approximately 50-90% V. jacobsoni mortality was observed 48 h after treatment with associated bee mortality lower than 10%. Although topically applied neem oil did not result in direct A. woodi mortality, it offered significant protection of bees from infestation by A. woodi. Other vegetable and petroleum-based oils also offered selective control of honey bee mites, suggesting neem oil has both a physical and a toxicological mode of action. Although oils are not as selective as the V. jacobsoni acaricide tau-fluvalinate, they nonetheless hold promise for the simultaneous management of several honey bee pests.

Animals↗

Toxicity of the herbicide glufosinate-ammonium to predatory insects and mites of Tetranychus urticae (Acari: Tetranychidae) under laboratory conditions.

The toxicities of the herbicide glufosinate-ammonium to three predatory insect and two predatory mite species of Tetranychus urticae Koch were determined in the laboratory by the direct contact application. At a concentration of 540 ppm (a field application rate for weed control in apple orchards), glufosinate-ammonium was almost nontoxic to eggs of Amblyseius womersleyi Schicha, Phytoseiulus persimilis Athias-Henriot, and T. urticae but highly toxic to nymphs and adults of these three mite species, indicating that a common mode of action between predatory and phytophagous mites might be involved. In tests with predatory insects using 540 ppm, glufosinate-ammonium revealed little or no harm to larvae and pupae of Chrysopa pallens Rambur but was slightly harmful to eggs (71.2% mortality), nymphs (65.0% mortality), and adults (57.7% mortality) of Orius strigicollis Poppius. The herbicide showed no direct effect on eggs and adults of Harmonia axyridis (Pallas) but was harmful, slightly harmful, and harmless to first instars (100% mortality), fourth instars (51.1% mortality), and pupae (24.5% mortality), respectively. The larvae and nymphs of predators died within 12 h after treatment, suggesting that the larvicidal and nymphicidal action may be attributable to a direct effect rather than an inhibitory action of chitin synthesis. On the basis of our data, glufosinate-ammonium caused smaller effects on test predators than on T. urticae with the exception of P. persimilis, although the mechanism or cause of selectivity remains unknown. Glufosinate-ammonium merits further study as a key component of integrated pest management.

Aminobutyrates↗

Population dynamics and within-plant distribution of the mite Calacarus flagelliseta (Acari: Eriophyidae) on papaya in Hawaii.

An important element in developing a management strategy for a new pest is the study of its seasonal dynamics and within-plant distribution. Here, we studied the mite Calacarus flagelliseta Fletchmann, De Moraes & Barbosa on papaya, Papaya carica L. (Caricaceae), in Hawaii to quantify 1) patterns of seasonal abundance, 2) its distribution across different vertical strata of the papaya canopy, and 3) shifts in its use of the upper versus the lower surfaces of papaya leaves. Nondestructive sampling conducted in two papaya plantings revealed that 1) populations of C. flagelliseta peak during the summer; 2) mites are most abundant in the middle and lower strata of the plant canopy, and least abundant on the youngest leaves found in the upper canopy; and 3) mites are found more predominantly on the upper leaf surfaces when overall population density peaks, suggesting that individuals move from the lower to the upper leaf surfaces when food resources on the lower leaf surface have been exploited by conspecifics. These results have significant implications for the development of sampling plans for C. flagelliseta in papaya.

Animals↗

Phylogeny and host specificity of Psoroptic mange mites (Acarina: Psoroptidae) as indicated by ITS sequence data.

We used molecular phylogenetic techniques to study the systematic relationships and host specificity of Psoroptes mange mites, which are pests of numerous domestic and wild ungulates. Phylogenetic analysis of DNA sequence data from the internal transcribed spacer region 1 (ITS1) of nuclear ribosomal DNA indicated that populations of Psoroptes are not host specific. Furthermore, the currently used taxonomy of Psoroptes is not concordant with the phylogeny derived from ITS1. During the course of the study, we discovered apparent paralogous ITS sequences within individual mites as a result of varying polymerase chain reaction reaction conditions. This finding concords with other studies of ITS and suggests a cautious approach when interpreting data from ITS sequences. Host DNA contamination was also found to be a significant problem in data collection, and we report on the development of methods to overcome the problems of contamination in parasitic mites.

Animals↗

Permethrin-impregnated mattress liners: a novel and effective intervention against house dust mites (Acari: Pyroglyphididae).

This study tested the efficacy of permethrin-impregnated mattress liners in reducing house dust mites in the homes of volunteers with no previous recorded history of asthma, atopic eczema, or perennial rhinitis. The field trial using permethrin-impregnated (450 mg/m2 of pure permethrin in polyester netting weighing 35 g/m2) mattress liners (n = 9) was conducted for 27 mo. The permethrin-impregnated bedding significantly reduced house dust mites in mattresses for at least 27 mo. Allergen concentrations were significantly lowered at 15-mo postintervention. No adverse side-effects were reported. This is a promising development in house dust mite control.

Animals↗

Eosinophil viability-enhancing activity in mite-sensitive bronchial asthma.

We examined the eosinophil viability-enhancing activity (EVEA) of peripheral blood mononuclear cells (PBMNCs) obtained from 6 patients with mite-sensitive bronchial asthma (BA) and 9 normal control subjects. Mite concentrations of 1 microgram/ml and 10 micrograms/ml significantly increased EVEA in PBMNC culture supernatants from BA patients compared with PBMNCs from normal control subjects (76.1 +/- 11.0% at 10 micrograms/ml and 56.3 +/- 16.0% at 1 microgram/ml vs 20.6 +/- 12.6% at 10 micrograms/ml and 7.4 +/- 2.3% at 1 microgram/ml; p < 0.05). The level of IFN-gamma in PBMNC culture supernatants in BA patients was 2.3 +/- 0.9 IU/ml and in normal control subjects 0.7 +/- 0.3 IU/ml. A combination of mAbs (anti-IL-3, anti-IL-5 and anti-GM-CSF, with or without anti-IFN-gamma) neutralized the EVEA (p < 0.001, p < 0.001, respectively). Dexamethasone (10(-8) M to 10(-5) M), cyclosporin A (10(-7) M to 10(-5) M) and FK506 (10(-8) M to 10(-6) M) significantly inhibited EVEA in BA patients (p < 0.05 to p < 0.001). The release of eosinophil cationic protein (ECP) from eosinophils in the presence of mite-stimulated PBMNC culture supernatants was higher in patients with bronchial asthma (569 +/- 147 micrograms/l) than in normal control subjects (203 +/- 99 micrograms/l; p < 0.05).

Adolescent↗

Serum IgE, skin and radioallergosorbent tests for house dust and mites in asthmatic children.

Thirty asthmatic children were given an intradermal skin test, total IgE measurements and the radioallergosorbent test (RAST) against house dust and house dust mites (Dermatophagoides pteronyssinus and Dermatophagoides farinae). Results of the intradermal skin test and RAST were highly positive with overall agreement in 95.6%. The correlation coefficient between the total IgE and the RAST to house dust was 0.35 (p less than 0.05). There was no significant difference between the total IgE and the RAST to D. pteronyssinus and D. farinae (p greater than 0.05). The highly significant correlation coefficient between house dust and house dust mites (D. farinae, p less than 0.0001; D. pteronyssinus, p less than 0.00001) was determined by RAST. House dust has been found to be the most important allergen in children in Taiwan. The role of mites as a possible allergen in house dust also showed a highly significant correlation in our study.

Adolescent↗

Correlation between serum IgE and specific IgE antibody titer to house dust mite in children with asthma.

Using the enzyme-linked immunosorbent assay (ELISA) for IgE specific to mites, we evaluated the relationship between total serum IgE levels and IgE antibodies specific to mite in 58 asthmatic children. Our results showed that there was a positive correlation between total serum IgE and IgE antibody specific to Dermatophagoides pteronyssinus (r = 0.57; p less than 0.001) and Dermatophagoides farinae (r = 0.59; p less than 0.001). There was also a significant correlation between D. pteronyssinus-specific IgE and D. farinae-specific IgE (r = 0.68; p less than 0.001). This suggests that there are common allergens between the two species. The close correlation between the ELISA assay and skin test suggests that the former will be useful for the diagnosis of mite allergy in asthma.

Adolescent↗

Atopy and house dust mite sensitization as risk factors for asthma in children.

Asthma is commonly described as an atopic disease in childhood, but some cases of this disorder do not fit this description. The aim of this study was to evaluate the frequency of atopy, asthma, and sensitization to house dust mites in children with allergic symptoms. This study was performed at the Severance Hospital of Yonsei University with patients who visited the allergy clinic for evaluation of nonspecific upper respiratory symptoms, typical symptoms of asthma, or a general health workup. The patients were divided into three age groups: 0-3 years (group 1), 4-7 years (group 2), and 8-12 years (group 3). Of the 1,244 children examined, 844 (67.8%) were atopic and 400 (32.2%) were non-atopic. The frequency of atopy and asthma increased with age. Asthma was diagnosed in the same proportion (64%) of atopic and non-atopic children. As risk factors for asthma symptoms, the positive values of house dust mite (HDM) sensitivity were significantly increased in groups 1, 2, and 3 to 53.5%, 68.9%, and 80.2%, respectively. A significant difference between the percentage of asthmatics sensitized to HDM and that of asthmatics not sensitized to HDM was found only in group 3. In conclusion, asthma is related to atopy with increasing age, and house dust mite sensitization seems to be an important determinant of asthma in older children in Korea.

Animals↗

Effects of the northern fowl mite, Ornithonyssus sylviarum (Canestrini and Fanzago), on fertility and hatchability of eggs from artificially inseminated White Leghorn hens.

Egg production of White Leghorn hens infested with the northern fowl mite, Ornithonyssus sylviarum (Canestrini and Fanzago), was as much as 15% lower than egg production of control hens. However, when these hens were artificially inseminated with semen from mite infested roosters, fertility nor hatchability was affected by the mite infestation.

Animals↗

The effects of the northern fowl mite, Ornithonyssus sylivarium on egg production and body weight of caged White Leghorn hens.

Egg production of caged White Leghorn hens with heavy (greater than 1000) populations of the northen fowl mite, Ornithonyssus sylviarum (Canestrini and Fanzago), was 5 to 15% less than egg production of control hens. Body weight was depressed in hens infested with mites before the hens came into full egg production but not when hens were infested after that time. Feed consumption was not affected by mite populations.

Animals↗

An evaluation of permethrin, carbaryl, and amitraz for the control of northern fowl mites on caged chickens.

In two trials at the University poultry farm, two formulations of the synthetic pyrethroid permethrin (Ectiban 5.0% EC and Ectiban .25% Livestock Dust) were compared against carbaryl and amitraz sprays for the control of northern fowl mite, Ornithonyssus sylviarum (Canestrini and Fanzago), on caged birds. Under continuous reinfestation pressure, birds treated with dust did not support live mites in the vent region until 7 weeks posttreatment in comparison to 4 weeks with the permethrin 0.5% spray. Initially, both carbaryl (.5%) and amitraz (0.5%) sprays were as effective in controlling mites as the permethrin spray but did not provide protection for as long a period. Individual birds were either had dusted or treated with low pressure sprays.

Animals↗

Effects of the northern fowl mite, Ornithonyssus sylviarum (Canestrini and Fanzago), on egg quality of White Leghorn hens.

Interior egg quality and shell thickness of eggs from hens infested with the northern fowl mite, Ornithonyssus sylviarum (Canestrini and Fanzago), were compared with eggs from mite free hens. Egg shells of mite infested hens were significantly thicker (P greater than or equal to .05) but within the normal biological range. There were no differences in either Haugh units or yolk color between the two groups of hens.

Animals↗

Control of the northern fowl mite on inanimate objects by fumigation: field studies.

Field studies were conducted to determine the efficacies of methyl bromide and sulfur dioxide as fumigants for northern fowl mite, Ornithonyssus sylviarum (Canestrini and Fanzago), on egg flats and egg cases. Both fumigants killed the mites within 24 hr; however, sulfur dioxide had a repulsive irritating odor, which was retained in the cardboard egg flats and egg crates and persisted for several days after exposure to the gas. When used in accordance with the manufacturer's recommendations for fumigating enclosed trailers and vans to control insects that attack food products, methyl bromide gave excellent control of northern fowl mites infesting inanimate materials.

Animals↗

A new in-cage treatment system for control of northern fowl mites on laying hens.

Commercially available plastic and other types of flexible tubing were tested to determine their relative permeability to an emulsifiable concentrate (EC) of permethrin acaricide. Bioassays employing northern fowl mites, Ornithonyssus sylviarum (Canestrini and Fanzago), indicated that .3 mg permethrin/cm2 surface area penetrated vinyl plastic tubing that had been filled with 42.5% active ingredient EC for 14 days. Vinyl tubing circuits were installed in wire poultry cages, either in the form of suspended loops or woven through the rear cage wall, and permethrin EC was introduced by means of a small pump. Significant reductions in northern fowl mite populations on hens caged therein were apparent after 48 days posttreatment. This approach has merit as a long-term, labor-economical form of mite control on caged poultry.

Animals↗

Cultures of house-dust mites on animal skin scales.

The main constituent of one of the most suitable media for growing house-dust mites (D. pteronyssinus) are human skin scales. In this study it is shown that skin scales of a number of other animals are also accepted as food by these mites, with evidence of allergen production. Production of house-dust mite allergen for diagnosis and treatment should not be done on non-human animal skin scales, because of their own high allergenicity.

Allergens↗

Efficacy of a pour-on formulation of doramectin against lice, mites, and grubs of cattle.

OBJECTIVE: To determine effectiveness of a pour-on formulation of doramectin against Damalinia bovis, Haematopinus eurysternus, Linognathus vituli, Solenopotes capillatus, Chorioptes bovis, Sarcoptes scabiei, Hypoderma bovis, and Hypoderma lineatum. ANIMALS: Cattle of various ages with naturally acquired or artificial infestations with 1 or more species of lice, mites, or grubs. PROCEDURE: In 10 louse and 6 mite studies, cattle were treated with doramectin (500 microg/kg, topically) on day 0, and parasite counts were performed approximately weekly from days 0 to 35. In 6 grub studies, cattle expected to harbor Hypoderma spp were treated before emergence of warbles. After warbles began to emerge, they were counted every 2 weeks, and grubs were collected and identified by species. RESULTS: Burdens of D bovis, H eurystemus, L vituli, and S capillatus on doramectin-treated cattle were 0 by 28 days after treatment. Burdens of C bovis and S scabiei decreased to 0 in naturally infested cattle and approximately 0 in artificially infested cattle by day 14 to 15. In grub studies, 107 of 136 control cattle had warbles, whereas 2 of 136 doramectin-treated cattle had 1 warble each, which represented a cure rate of 98.5%. CONCLUSION AND CLINICAL RELEVANCE: One topical application of doramectin was highly efficacious against common species of lice, mites, and grubs known to affect performance, health, and appearance of cattle.

Administration, Topical↗