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At least 1,495 records · Page 83Linked to original sources
PubMed1975

[Detection of pathogenic leptospira in the waste water and sewage sludge of large pig breeding sites].

Sewage effluent and sludge from the purification plant of 8 large piggeries was examined for the presence of pathogenic leptospires. By using the methods of Appelman and Van Thiel it was found that 43.1% of samples of effluent were contaminated with L. pomona and O. tarassovi. Altogether 33 strains of pomona and three mixed cultures of pomona and tarassovi were obtained. The isolated strains were shown to be pathogenic by experimental infection of guinea-pigs, rabbits and pregnant and non-pregnant sows. The average period of survival of pathogenic leptospires in sewage effluent was 24 to 48 hours, with a maximum of 96 hours. Leptospires were killed within 24 hours in decanted sludge, owing to its strong acidity.

Animals↗
PubMedDate not supplied

[Research of beta-galactosidase in the leptospira].

The Authors have investigated on the presence of beta-galactoxidase in the serotypes of Leptospires most frequently employed for the diagnosis of the formes epidemiologic occurrent in Italy. With the two employed methodes it was possible to make evident the enzyme in all the serotypes, but not in L. saxkoebing (Mus 24); L. ballum (Castellon 3), L. doberdò (Percedol 3).

Galactosidases↗
PubMed2002

The construction and expression of recombinant shuttle plasmid with OmpL1 gene from leptospira interrogans serovar Lai strain 017 in Bacille Calmette-Guerin.

OBJECTIVE: To construct recombinant BCG against leptospirosis. METHODS: We amplified the entire open reading frame of the OmpL1 gene from the genome of the leptospire serovar Lai strain 017. Two recombinant plasmids pBQ1 and pBQ2 were constructed by oriented ligation based on the E. coli-BCG shuttle plasmids pMV261 and pMV361 respectively. The recombinant plasmids were transformed into BCG by electroporation. The rBCGs bearing pBQ1 and pBQ2 were induced by high temperature of 45 degrees C. RESULTS: The expressed product, a 35kD protein was detected by SDS-PAGE. The result indicates that pBQ1 and pBQ2 can express OmpL1 in rBCG. CONCLUSION: The technical methods in this study may help detect the immunogenicity and immunoprotection of OmpL1 and develop more safe, highly effective rBCG bearing leptospiral antigen with long-lasting protection.

BCG Vaccine↗