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PubMed1985

PZ-peptidase activity from ejaculated boar spermatozoa.

Protein constituents of the boar spermatozoon were fractionated in three components, the hypotonic soluble fraction, the detergent-soluble fraction, and the detergent-insoluble fraction. When all these fractions were assayed spectrophotometrically using the PZ-peptide as substrate, a high value of PZ-peptidase specific activity was observed in the first fraction. Electrophoretic analysis at pH 8.3 of the protein content from the hypotonic soluble fraction revealed the existence of multiple molecular forms capable of hydrolysing the PZ-peptide. The major form was characterized by a surprisingly high value of electrophoretic mobility, index of the presence of numerous negatively charged residues. Biochemical and ultrastructural analyses showed that the hypotonic soluble fraction did not contain intrinsic, and specifically acrosomal, sperm enzymes.

Animals↗
PubMed2001

Use of computer-assisted sperm motility assessment and multivariate pattern analysis to characterize ejaculate quality in Mohor gazelles (Gazella dama mhorr): effects of body weight, electroejaculation technique and short-term semen storage.

Subjective and objective semen assessments were performed on 18 male Mohor gazelles (Gazella dama mhorr). Sperm motility assessments combined with sperm plasma membrane and acrosomal integrity evaluations were undertaken as part of a captive breeding programme. The primary objective was to test methodology for short-term preservation of gazelle semen for artificial insemination (storage in N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulphonic acid-Tris diluent (TEST) for up to 96 h at 17 degrees C). However, the secondary objective was to investigate phenotypic and genotypic influences on semen quality within this small population, which was established in 1971 with only 12 genetic founders. Sperm motility was measured by computer-assisted semen assessment and the data were analysed using a pattern analysis technique to detect and quantify naturally occurring sperm subpopulations within the semen samples. Four sperm subpopulations distinguishable by their motion characteristics were detected. The relative frequencies of two subpopulations (population 2: highly motile, non-linear; and population 4: poorly motile, non-linear) in fresh semen were correlated with the maximum voltage used during electroejaculation. The frequency of subpopulation 2 was negatively correlated with maximum voltage (r = -0.875, P < 0.0001) and the frequency of subpopulation 4 was positively correlated (r = 0.727, P < 0.005). The frequencies of all subpopulations varied significantly among the animals sampled (chi-squared = 2577.6, degrees of freedom = 54, P < 0.0001) and subpopulation 4 was also correlated with body weight (r = -0.59, P < 0.005). Semen stored at 17 degrees C retained motility, plasma membrane and acrosomal integrity for 48 h, but these measures decreased thereafter. The frequency of a sperm subpopulation showing uncoordinated but active motility increased significantly over the first 48 h and then decreased.

Animals↗