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Effects of induced charge in the kinestatic charge detector.

The principle of the kinestatic charge detector (KCD) for digital radiography depends on the synchronization of the scan velocity of a parallel plate drift chamber with the cation drift velocity. Compared with line-beam scanners, this motion-compensated imaging technique makes better use of the x-ray tube output. A Frisch grid traditionally has been used within the KCD to minimize unwanted signal contributions from both cations and negative charge carriers during irradiation. In this work the charge induction process in a parallel plate geometry was investigated for the special case of the KCD. In the limit of infinite plates, the cathode charge density due to both cations and negative charge carriers increases quadratically in time for a kinestatically scanned narrow slit. In the KCD the cathode is segmented into an array of narrow electrodes, each aligned with the incident x-ray beam. Our conformal mapping computation determined that the shape of the induced charge signal depends critically on delta x/w, the ratio of electrode width to drift gap. Our conclusion introduces the possibility of eliminating the Frisch grid from the KCD design because the value of delta x/w required for transverse sampling in the KCD is sufficiently low as to allow "self-gridding" to take effect.

Humans↗

Off-axis x-ray spectra: a comparison of Monte Carlo simulated and computed x-ray spectra with measured spectra.

The off-axis x-ray spectra from a constant potential x-ray generator were measured with a high purity germanium spectrometer cooled to liquid nitrogen temperature. The measured spectra were compared with off-axis x-ray spectra calculated using a code based on the semiempirical model developed by Tucker et al. and Monte Carlo simulated x-ray spectra using the EGS4 code system. In this study, both the Tucker model, and the EGS4 code system, were found to produce off-axis bremsstrahlung x-ray spectra which agreed well with the spectra measured at three emerging angles. In the measured and the EGS4 generated spectra the total K-characteristic peaks were in increasing order, as observed in the anode to cathode direction, whereas the Tucker model produced maximum total K-characteristic peaks at the 6 degrees anode side, and lesser amounts at the central axis and the 6 degrees cathode side. Large differences in the total K-characteristic lines is seen among the three different methods. The EGS4 code system was able to produce x-ray spectra for a combination of target materials.

Computer Simulation↗

Oxygen tension changes evoked in the brain by visual stimulation.

Localized changes in oxygen tension were recorded with platinum cathodes placed in the lateral geniculate nucleus in both anesthetized and awake cats. The amplitude of the responses increased with increasing stimulus intensity, but decreased with increasing flash rate. Both increases and decreases in cathode current were produced by steady illumination. The characteristics of the responses suggest that the responses reflect localized variations in blood flow, produced in turn by changes evoked in the tonic neural activity of the lateral geniculate nucleus.

Animals↗

Iontophoretic application of adenine arabinoside monophosphate to herpes simplex virus type 1-infected hairless mouse skin.

Several antiviral agents were applied topically or by iontophoresis to hairless mouse skin inoculated with herpes simplex virus type 1 (HSV-1), and the chemotherapeutic effectiveness was evaluated. Topical application of iododeoxyuridine, arabinoside A, and adenine arabinoside monophosphate (ara-AMP) moderately decreased the average lesion score, number of mice with paralysis, and number of mice dying in HSV-1-infected animals. Also, the mean survival time was moderately prolonged by topical application of those antiviral agents. When ara-AMP was applied by cathodal (-) iontophoresis to the HSV-1-infected skin, the average lesion score, number of mice with paralysis, and number of mice dying were greatly decreased. Furthermore, the mean survival time of mice was highly increased by cathodal (-) iontophoresis of ara-AMP. The therapeutic efficacy of ara-AMP iontophoresis was much superior to the topical application of iododeoxyuridine, arabinoside A, and ara-AMP. These data suggest that ara-AMP iontophoresis would be the method of choice for the management of HSV-1 skin lesions in hairless mice.

Animals↗

Influence of electric fields and pH on biofilm structure as related to the bioelectric effect.

Mixed species biofilms of Klebsiella pneumoniae, Pseudomonas fluorescens, and Pseudomonas aeruginosa were grown in a flow cell fitted with two platinum wire electrodes. The biofilm growing on the wires reached a thickness of approximately 50 microm after 3 days. When a voltage was applied with oscillating polarity, the biofilm attached to the wire expanded and contracted. The biofilm expanded by approximately 4% when the wire was cathodic but was reduced to 74% of the original thickness when the wire was anodic. The phenomenon was reproduced by alternately flushing the flow cell with media adjusted to pH 3 and pH 10 with no electric current. At pH 10 the biofilm was unaltered, but it became compacted to 69% of the original thickness at pH 3. We explained these phenomena in terms of the molecular interactions between charged acidic groups in the biofilm slime and the bacterial cell walls. Contraction of the biofilm under acidic conditions may be caused by (i) the elimination of electrostatic repulsion from neutralization of negatively charged carboxylate groups through protonation and (ii) subsequent hydrogen bonding between the carboxylic acids and oxygen atoms in the sugars. Electrostatic interactions between negatively charged groups in the biofilm and the charged wire may also be expected to cause biofilm expansion when the wire was cathodic and contraction when the wire was anodic. The consequences of the explanation of the increased susceptibility of biofilm cells to antibiotics in an electric field, the "bioelectric effect," are discussed.

Analysis of Variance↗

Effect of pH on Anaerobic Mild Steel Corrosion by Methanogenic Bacteria.

Methanogens can use H(2) produced by cathodic depolarization-mediated oxidation of elemental iron to produce methane. Thermodynamic consideration of the cathodic depolarization mechanism predicts more oxidation of Fe at lower pH. Methanogenic responses to pH by Methanococcus deltae, Methanococcus thermolithotrophicus, and Methanosarcina barkeri were examined. When grown on H(2)-CO(2), these bacteria had pH optima from 6.2 to 7.0, but when all H(2) was supplied from Fe, methanogenic pH optima were lower, 5.4 to 6.5. Corrosion was monitored with and without cultures and at various pHs; more corrosion occurred when cultures were present, biologically induced corrosion was greatest at the pH optima for methanogenesis from Fe, and corrosion without cultures increased with a drop in pH.

Journal Article↗

BIOELECTROCHEMICAL ENERGY CONVERSION.

The interaction between photosynthetic microorganisms and an inert electrode material was examined. Cathodic polarization values of platinum-bearing marine algae were obtained over a wide current-density range under both illumination and dark conditions. A potential shift of 0.6 v in the cathodic direction occurred upon illumination at a current density of 4.3 mua/cm(2). Similar photo-induced results, involving anodic polarization, were obtained by use of resting cells of Rhodospirillum rubrum supplemented with malate. Appropriate combinations of such bioelectrodes were used to assemble an electrochemical cell capable of light-dependent production of electrical energy.

Electrophysiology↗

Effect of the surface composition of motile Escherichia coli and motile Salmonella species on the direction of galvanotaxis.

We have reported that motile Escherichia coli K-12 placed in an electric field swims toward the anode but that motile Salmonella typhimurium strains swim toward the cathode, a phenomenon called galvanotaxis (J. Adler and W. Shi, Cold Spring Harbor Symp. Quant. Biol. 53:23-25, 1988). In the present study, we isolated mutants with an altered direction of galvanotaxis. By further analyses of these mutants and by examination of E. coli and Salmonella strains with altered cell surface structure, we have now established a correlation between the direction of galvanotaxis and the surface structure of the cell: motile rough bacteria (that is, those without O polysaccharide; for example, E. coli K-12 and S. typhimurium mutants of classes galE and rfa) swam toward the anode, whereas motile smooth bacteria (that is, those with O polysaccharide; for example, wild-type S. typhimurium LT2) swam toward the cathode. However, smooth bacteria with acidic polysaccharide capsules (K1 in E. coli and Vi in Salmonella typhi) swam toward the anode. Measurements of passive electrophoretic mobility of strains representative of each set were made. We propose that the different directions of galvanotaxis of rough (or capsulate) bacteria and of smooth bacteria are explicable if the negative electrophoretic mobility of flagellar filaments is less than that of rough bodies but greater than that of smooth bodies.

Bacterial Capsules↗

Cationic proteins of polymorphonuclear leukocyte lysosomes. I. Resolution of antibacterial and enzymatic activities.

Zeya, H. I. (University of North Carolina, Chapel Hill), and J. K. Spitznagel. Cationic proteins of polymorphonuclear leukocyte lysosomes. I. Resolution of antibacterial and enzymatic activities. J. Bacteriol. 91:750-754. 1966.-A lysosomal fraction from polymorphonuclear (PMN) leukocytes of guinea pig peritoneal exudate was subjected directly to electrophoresis on cellulose acetate paper treated with cetyltrimethyl ammonium bromide. The Iysosomal components resolved into seven bands moving towards the cathode. Assay of the eluted bands showed that the antibacterial activity was distinct from lysosomal enzymes and was associated with three cationic components (bands I, II, and III) which migrated most rapidly towards the cathode, ahead of lysozyme ribonuclease and deoxyribonuclease. Qualitatively, the antibacterial components appeared to be rich in arginine. The antibacterial components were absent in the pherograms of nuclear fractions of PMN leukocytes and in supernatant fractions that remained after lysosomes were removed from cell homogenates by centrifugation at 8,000 x g.

Animals↗

Identification of staphylococcal hemolysins by an electrophoretic localization technique.

A technique for identifying and characterizing staphylococcal hemolysins by first separating them electrophoretically in barbital-buffered agar gel (pH 8.4) at 5 ma/cm for 2 hr and then determining their hemolytic activities by exposing them to human, horse, rabbit, and sheep erythrocytes is described. The alpha-hemolysin produced by a White variant of the Wood 46 strain of Staphylococcus aureus migrated 18 mm towards the cathode, and it lysed horse, rabbit, and sheep erythrocytes, whereas a Clear variant of the Wood 46 strain of S. aureus produced a lysin which migrated similarly to the alpha-hemolysin but lysed only rabbit cells. This latter lysin was tentatively named alpha(1)-lysin. This strain of S. aureus also produced beta-hemolysin which migrated 36 mm towards the cathode and lysed sheep cells. beta-Hemolysin produced by some strains of S. aureus showed considerable tailing during electrophoresis, whereas beta-hemolysin produced by other strains of S. aureus migrated as a well-defined peak. A lysin migrating 11 mm towards the anode was probably delta-lysin. It was, however, not produced in sufficient concentration when the cultures were grown in semisolid medium.

Animals↗

Leukocyte lactate dehydrogenase changes as an indicator of infection prior to overt symptoms.

Lactic acid dehydrogenase (LDH) concentration of rabbit serum and leukocytes was followed during the course of an acute infection with Diplococcus pneumoniae. Control values were obtained prior to infection, and again 4, 24, and 48 hr later. LDH isozymes were characterized by acrylamide gel electrophoresis and quantitated by densitometry. An increase in serum LDH was observed as early as 4 hr after infection. These levels returned to normal in 24 hr and rose again 48 hr after infection. The LDH level of leukocytes, from 10 of 12 infected rabbits, rose rapidly during the 24-hr period after exposure. The levels were two to three times the original preinoculation level for that animal. In six of these rabbits, this LDH elevation occurred 4 hr after inoculation and preceded the onset of fever. Change in the type of leukocyte did not account for the increase in cellular LDH. All infected rabbits showed approximately the same increase in polymorphonuclear leukocytes, but not all developed comparable increases in LDH. The isozyme patterns obtained, when defined amounts of enzyme were applied to the gel for electrophoresis, were characterized for the most part by a three-enzyme pattern. Increasing amounts of enzyme occasionally revealed a fourth, more cathodal, enzyme. The more cathodic enzymes appear to be the most responsive when sudden shifts in enzyme concentration occur within the cell.

Animals↗

Microbiology subsystem of a total, dedicated laboratory computer system.

The computer system used by the Microbiology Service of the Clinical Pathology Department, Clinical Center, National Institutes of Health is discussed. This microbiology subsystem is a part of a dedicated on-line laboratory computer system used by the entire department. The laboratory computer is connected on-line to a hospital computer which provides patient admission, transfer, and discharge data. Mark sense worksheets and cathode ray tube terminals are used for result entry and correction. Cumulative patient reports are printed. Results for both active and completed accessions can be easily retrieved on cathode ray terminals in the laboratory. All laboratory data are archived on magnetic tape from which a research data base and microfiched laboratory records are generated. The manner in which the system is integrated in the routine operation of the microbiology laboratory is emphasized. In addition, some of the costs, benefits, liabilities, and pitfalls associated with the introduction of the computer in the laboratory are reviewed. Finally, we have presented our concept of some of the future enhancements to our present system and some of the directions in which any future microbiology system might develop.

Computers↗

Detection of Legionella pneumophila capsular-like envelope antigens by counterimmunoelectrophoresis.

The capsular-like envelope of Legionella pneumophila strains Togus 1 (serotype 2) and Philadelphia 1 (serotype 1) was isolated and purified by column chromatography on Sepharose 6B. Antibody raised in rabbits to these two antigenic materials did not cross-react in gel diffusion. Upon electrophoresis followed by gel diffusion, the majority of both envelope materials was found to migrate towards the cathode. A minor antigenic component of each envelope only migrated slightly towards the anode. Using the envelope antigens and the two anti-envelope sera in a counterimmunoelectrophoresis (CIE) assay, positive results were only obtained when the antigenic materials were placed in the cathodal well. The Togus 1 and Philadelphia 1 antigens did not cross-react in CIE. The sensitivity of the CIE assay was poor (15.6 micrograms/ml by carbohydrate content) compared to its sensitivity in other microbial systems. Although CIE may not be a useful diagnostic aid in identifying Legionella species due to its low sensitivity, it may be of value in serotyping the microorganism since we did not see cross-reactivity between the two strains when anti-envelope sera were used.

Animals↗

Carbohydrate deficient serum transferrin in a new systemic hereditary syndrome.

Four patients with a new, inherited, complex developmental deficiency syndrome were studied. The syndrome affects the central and peripheral nervous system, and also the retina, liver, bone, adipose tissue, and genital organs. Abnormalities of glycoproteins, glycopeptide hormones, and lipids have been found in serum from these patients, the most pronounced being increased cathodal forms of transferrin. Isoforms of serum transferrin were therefore analysed qualitatively and quantitatively by isoelectric focusing and isocratic anion exchange chromatography, and the carbohydrate composition was determined in transferrin isolated by immune affinity chromatography. All the patients had about tenfold raised serum concentrations of isotransferrins with higher isoelectric points than normal. Similar findings, though less pronounced, were made in all the fathers and in one of the mothers. Half the transferrin in the patients was constantly present in two principal abnormal cathodal forms in approximately equal amounts. Carbohydrate determinations in purified transferrin showed quantitatively similar deficiencies of sialic acid, galactose, and N-acetylglucosamine, the mannose content being normal. The results suggest that either two or all of the normally four terminal trisaccharides in transferrin may be missing. A defect in the synthesis or catabolism, or both, of this trisaccharide, which is common to many secretory glyco-conjugates, is likely. Apart from providing a quantitative diagnostic method, the present findings may serve as a basis for further studies of the metabolic deficiency in this syndrome.

Biomarkers↗

Electrophoretic separation and differentiation of enzymes from human and from porcine liver.

The electrophoretic separations of some human and pig liver enzymes on cellulose acetate and Cellogel were investigated, with reference to their joint occurrence in serum of patients undergoing treatment by extracorporeal pig liver perfusion. In every case it was possible to distinguish between the human and pig enzymes. Pig lactate dehydrogenase isoenzymes occupy a position slightly anodic to the corresponding human bands. The aspartate transaminase band of human is more anodic than that of pig, but their cathodic bands have the same mobility. Alanine transaminase of both human and pig liver extract is shown to exist as two bands each towards the anode. The faster moving human band is more anodic than the corresponding pig band, while the other human band is less anodic. Sorbitol dehydrogenase, alkaline phosphatase, and ornithine carbamoyltransferase all exist as one band each. Human sorbitol dehydrogenase is more cathodic than the pig enzyme, human alkaline phosphatase more anodic than the pig enzyme, while human ornithine carbamoyltransferase is less anodic than the pig enzyme.

Alanine Transaminase↗

Stimulus intensity and site of excitation in human median nerve sensory fibres.

Median nerve sensory fibres were stimulated with minimal and supramaximal stimuli at the base of the third digit in 30 normal subjects. Evoked nerve action potentials were simultaneously recorded from two points on the median nerve just above the wrist. As stimulus voltage was increased from minimal to supramaximal, amplitudes of nerve action potentials increased, latencies decreased, but conduction velocities remained unchanged. The shortening of latencies was interpreted as movement of the effective point of nerve excitation away from the stimulating cathode towards the recording electrodes. Therefore, the effective point of nerve excitation cannot be assumed to be underneath the cathode, but at some distance from it depending on stimulus intensity. Furthermore, the fastest conducting sensory fibres in the human median nerve do have a lower threshold than slower conducting fibres.

Action Potentials↗

Magnetic and electrical stimulation of cervical motor roots: technique, site and mechanisms of excitation.

Cervical motor roots and the brachial plexus were excited transcutaneously with magnetic (MagStim) and electrical stimulation (ElStim) applied dorsally over the spine and over the supraclavicular fossa (Erb's point). The compound muscle action potentials (CMAPs) from the abductor digiti minimi (ADM) and the biceps muscles (BICEPS) could be evoked with either stimulating technique in all 52 subjects tested. With MagStim over the spinous process C7, greater CMAPs were obtained from ADM (p less than or equal to 0.0001, paired t test) and BICEPS (p less than or equal to 0.005) when the inducing current in the coil as viewed from behind was clockwise for the right arm and vice versa. ElStim with the cathode over C7/T1 and the anode directed cranially provided greater CMAPs from the ADM (p less than or equal to 0.0001) and smaller CMAPs from the BICEPS (p less than or equal to 0.01) than with the inverse polarity. MagStim of the cervical roots provided CMAPs which were smaller from ADM (p less than or equal to 0.0001), and greater from BICEPS (p less than or equal to 0.0001), than ElStim (cathode C7/T1), whereas latencies did not differ significantly (p less than or equal to 0.3). When comparing ElStim and MagStim applied over Erb's point, the former yielded greater CMAPs and 0.5 ms longer latencies from both the ADM and BICEPS (p less than or equal to 0.001). From these data and additional studies in four patients, including direct intraoperative root stimulation in one of them, it is concluded that ElStim and MagStim over the spine excite the motor roots at a similar site, that is, within a few cm outside the intervertebral foramina. The site of stimulation is difficult to predict and depends on the placement of the stimulating devices and the intensities used. In contrast, MagStim of the brachial plexus over Erb's point occurs on average about 3.5 cm distal to the site of ElmStim.

Adult↗

The demonstration of transmissible gastroenteritis viral antigens by immunoelectrophoresis and counterimmunoelectrophoresis.

Immunoelectrophoresis of alkaline intestinal extracts, or the supernatants after precipitation of these extracts with 60% ammonium sulfate, prepared from piglets experimentally infected with the DL or Purdue strains of transmissible gastroenteritis virus, revealed up to three antigens. Two antigens migrated towards the anode, and the third migrated towards the cathode. Antigens with anodal or cathodal mobility were also demonstrated in the same materials by counterimmunoelectrophoresis, and the procedure for counterimmunoelectrophoresis was modified to detect both antigens in a single test.

Animals↗