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Racial identification from the midfacial skeleton with special reference to American Indians and whites.

Successful approaches to race determination of unidentified human remains have been developed by anatomists and physical anthropologists, but few quantitative methods are available for distinguishing American Indian crania from those of whites. The leading method in use today is particularly ineffective in its placement of American Indian skulls from the western regions of the United States. Recent development and testing of a new metric method suggests a much more effective technique. The method involves six breadth and projection measurements of the midfacial skeleton, the calculation of three indices, and a simple direct reading of results. The method has the additional advantage of use in the autopsy room with minimal dissection of soft tissue required. Based upon a less extensive test of East Asian and Arctic Mongoloid crania, the method appears to be even more effective in separating them from the sharp featured whites. Larger samples of American blacks and Polynesians are presently under study and these also appear to separate quite readily from whites using the same or similar sectioning values.

Cephalometry↗

Pattern of vascular canals in the bone extremities of the pelvic appendicular skeleton in broiler type fowl.

Broiler fowls were reared from hatching till 20 weeks old. Birds were killed throughout the growth period and the bone extremities of the pelvic appendicular skeleton prepared for study. The patterns of vascular canals in these broiler fowls were very similar to earlier reports in laying strain fowls. There was a great range of and variation in the vascular morphology of broiler fowl. The physeal vasculature of the rapidly growing broilers was frequently disrupted, and such areas were often associated with dyschondroplastic lesions. The rapidly growing bone extremities in broiler fowls were susceptible to occlusion of epiphyseal vascular canals. lesions were frequently seen at similar sites in different specimens. Links are suggested between the growth rate, weight and behavioural characteristics of broiler fowls and the development of lesions.

Animals↗

[Morphology and development of the cranium of Felis silvestris f. catus Linné 1758--IV: Visceral skeleton, membrane bones: summary, conclusions and bibliography].

The present last part of the study on morphology and development of the cranium of Felis silvestris f. catus provides a detailed description and discussion of the development of the visceral skeleton and the membrane bones. Finally, there is given a summary with conclusions of the whole investigation. In Felis 2, the Cartilago Meckeli is still in homocontinuous connection with the Malleus. Probably, only the rostral part of the Cartilago will ossify enchondrally, while the caudal part shows some beginning resorption. In this 95 mm Embryo, the Cartilago Reicherti still remains cartilaginous. A small cartilaginous Spence's cartilage lies in dorsolateral position towards the Chorda tympani. All membrane bones of the adult cranium are present in Felis 1. The following development of the osseous skull towards the stage of Felis 2 is characterized by covering the wide fissures of the side wall and by resorption of certain primordial supporting constructions. The interparietal develops from a paired anlage. In Felis 1 and 2, the cartilago within the pterygoid probably serves as a gliding surface for the M. tensor veli palatini. The dental bone contains some largely extended particles of secondary cartilage.

Animals↗

Adamantinoma of the appendicular skeleton--updated.

An updated review of adamantinoma of the appendicular skeleton now provides 195 well-documented cases from the world literature. An additional five new cases are added. Statistically, the tumor remains unusually prevalent in the tibia, but all other major limb bones have been involved, and involvement of several short bones rarely has been reported. The neoplasm is more commonly found in males, but higher earlier age incidence is found in females. The frequent history of preceding trauma may indeed be important in tumor formation. The histogenesis of the tumor is now considered to be epithelial in origin by ultrastructural and immunohistochemical methods. A high incidence of recurrence or metastases is found with inadequate cancer surgery. Known mortalities have indicated severe metastatic disease by aggressive-appearing cells. Previously, early amputation had provided good results, but wide excision or segmental resection with grafting techniques are equally successful. The recent work with allograft replacement of a widely excised segment has shown good early results.

Adolescent↗

Effect of BCG cell-wall skeleton on metastasis of syngeneic tumor in rats, with special reference to lymphocyte trapping.

Oil-attached BCG cell-wall skeleton (BCG-CWS) was demonstrated to have an activity inducing lymphocyte trapping in the draining node in rats. It acts also as a potent adjuvant for the lymphocyte trapping when injected into the growing syngeneic transplantable tumor. Treatment with repeated intratumor injections into the primary tumor resulted in suppression of tumor growth in both primary and metastatic sites. Even when the primary tumor escaped regression, inhibitory effect on metastatic spread was attained by the therapy. The contribution of BCG-CWS to suppression of metastasis especially in the draining node was discussed from the point of lymphocyte trapping in the draining node.

Animals↗

A clinical trial of cell-wall skeleton of BCG in chemoimmunotherapy of acute leukemia.

A clinical trial of chemoimmunotherapy using cell-wall skeleton of BCG (BCG-CWS) was conducted in 28 patients with acute leukemia in complete remission. Chemotherapy consisted of monthly intensification therapy for 2 months and bimonthly therafter. Immunotherapy with 200 microgram of oil-attached BCG-CWS mixed with 10(7) autochthonous leukemic cells was given intradermally at either of upper or lower extremities every week, except when the patients were on maintenance therapy. No serious systemic side effect attributable to BCG-CWS was noted. Six patients developed mild and transient temparature elevation. Most importnat side effect was local skin reaction. Indulated papules developed in all patients, resulting in draining ulcerations in 26 patients. Increase of immunological reactivity of the patients receiving BCG-CWS was noted. Skin test response to PPD, Varidase, and candida extract showed definite increase. In vitro lymphocyte blastogenic response to PPD, concanavalin-A, and pokeweed mitogen revealed significant increase.

Adolescent↗

Effect of Nocardia rubra cell wall skeleton on humoral and cellular factors related to immune response in mice and guinea pigs.

The in vitro and in vivo effects of Nocardia rubra cell wall skeleton (N-CWS) on induction of serum ceruloplasmin, hemopexin, haptoglobin, complement and interferon in mice and guinea pigs are described. After intraperitoneal administration of N-CWS to mice, serum hemopexin increased markedly and also induced an increase of haptoglobin, but not of ceruloplasmin. The drug activated complement in vitro but did not affect serum complement level in guinea pigs. Intravenous N-CWS induced serum interferon in mice, although there were strain differences. Addition of N-CWS to mouse spleen cell culture also induced interferon. Intraperitoneal N-CWS increased peritoneal cells in mice: in particular polymorphonuclear leukocytes increased soon after dosing, followed by macrophages and lymphocytes. However, the drug did not affect peripheral leukocyte counts or alter peripheral cell population.

Animals↗

Studies on hormonal regulation of the growth of the craniofacial skeleton: IV. Specific binding sites for glucocorticoids in condylar cartilage and their involvement in the biological effects of glucocorticoids on cartilage cell growth.

Using a whole-tissue binding assay and cell-free binding measurements indicated the presence of a specific steroid receptor for triamcinolone in cartilage cells of neonatal mouse mandibular condyle. Analysis of receptor levels showed that whole-tissue preparations bound 1360 fmol triamcinolone/mg protein. Affinity measurements revealed a dissociation constant of 7.6 X 10(-9) M. There was a close correlation between triamcinolone inhibition of DNA synthesis and steroid occupancy of whole-tissue receptors. The inhibitory effect of triamcinolone upon DNA synthesis could be significantly reduced by "blocking" the respective receptors with cortexolone. All the cartilage cells in the condyle revealed distinct intracellular labeling using [3H] dexamethasone autoradiography. Hence, neonatal condylar cartilage, an active site of endochondral bone formation in the craniofacial skeleton, can be regarded as a genuine target tissue for the biological effects of glucocorticoids.

Animals↗

Antitumor activity of peritoneal exudate cells induced by cell-wall skeleton of Mycobacterium bovis BCG.

Peritoneal exudate cells (PEC) induced by oil-attached cell-wall skeleton of Mycobacterium bovis BCG (BCG-CWS) in ACI/N rats were tested for their effect on both in vivo and in vitro growth of syngeneic fibrosarcoma cells (AMC-60). Treatment of rats with intraperitoneal injections of BCG-CWS induced regression of syngeneic ascites tumor and increased the number of survivals. Whole PEC and adherent PEC from rats injected intraperitoneally with BCG-CWS inhibited the uptake of tritiated thymidine into the fibrosarcoma cells in an in vitro cytostasis test. This in vitro cytostatic effect was more marked as the ratio of effector to target cells increased. In addition, when tumor cells were inoculated subcutaneously with BCG-CWS activated PEC, tumor takes decreased markedly. Oil-stimulated PEC and normal peritoneal resident cells were inactive in inhibition of tumor growth in vivo and in vitro.

Animals↗

Red cell comets: ultrastructure of axial elongation of the membrane skeleton.

In order to study intact red cell membrane skeletons, we developed two methods for the electron microscopic study of stress-ruptured and partially opened glutaraldehyde pre-fixed Triton shells of red cell ghosts. Both methods showed continuous networks of fine filaments decorated and apparently crosslinked by elongated particles. Our electron micrographs show a filamentous component that is morphologically consistent with F-actin. Quantitative considerations, including the dimensions of the elongated particles and published estimates of the number of particles in situ, suggest that compact spectrin decamers or dodecamers may exist in intact red cells. Long filaments consistent with F-actin appear to be decorated with the putative spectrin particles.

Actins↗

Protein kinase C phosphorylates a recently identified membrane skeleton-associated calmodulin-binding protein in human erythrocytes.

A membrane skeleton-associated protein with calmodulin-binding activity recently has been purified and characterized from human erythrocytes (Gardner, K. and Bennett, V. (1986) J. Biol. Chem. 261, 1339-1348). This new protein (CaM-BP103/97) has now been identified as a major substrate for protein kinase C in erythrocytes since phosphorylation of both of its subunits (Mr = 103,000 and 97,000) is elevated 3-15-fold in the presence of the phorbol ester, 12-O-tetradecanoylphorbol beta-acetate (TPA), under the following conditions: ghost membranes incubated with protein kinase C purified from rat brain, ghost membranes from erythrocytes pretreated with TPA, and intact erythrocytes metabolically labeled with 32PO4 and stimulated by TPA. The sites of phosphorylation of this protein by exogenous and endogenous protein kinase C are identical since two-dimensional 32P-peptide maps of both subunits labeled by either endogenous or exogenous enzyme are indistinguishable. Each subunit of CaM-BP103/97 accepts up to 3 mol of phosphate/polypeptide chain. In the presence of low calcium concentrations and in the absence of cytosol, the phosphorylation of CaM-BP103/97 is, on a molar basis, equal to or greater than that of proteins 4.1 and 4.9. As a target for both calmodulin and protein kinase C, CaM-BP103/97 is likely to play a key role in the effect of calcium on erythrocyte membrane shape and stability.

Adenosine Triphosphate↗

Calcification of the rabbit fetal skeleton.

The normal development of calcification in the fetal skeleton was studied in New Zealand White rabbits. The calcification process, confirmed by alizarin staining, was followed during days 16-30 of pregnancy. The process starts in the clavicle, most probably between day 15 and day 16. Simultaneously, calcification was observed in the mandible. In most of the bones of the skull, calcification starts during days 18-22. In the pectoral girdle and forelimbs the process starts in the diaphyses of the scapula, humerus, ulna and radius during days 16-19 and in the metacarpals and phalanges during days 21-26, the distal phalanges being the first to be calcified. The pelvic girdle and hindlimb follow a corresponding pattern. Calcification of the ribs commenced between days 18 and 20, and that of the vertebral column between days 19 (the atlas) and 28 (the last caudal vertebrae).

Animals↗

[Composition, structure and various properties of DNA-protein complexes located at the sites of DNA loop attachment to the nuclear skeleton].

The intimate structure of the complexes located at the sites of DNA loops attachment to the nuclear skeleton was analysed. It is shown that: there are at least three components of the attachment site complex: DNA, protein, RNA; protein moiety consists of 7-8 species with Mr 70-17 kDa. Their association with DNA is resistant to ionic detergents, high salt and urea treatments. The DNA-protein complex is also resistant to the SDS-pronase-phenol deproteinisation procedure; the buoyant density of the complex is the same as DNA density. RNase digestion at low ionic strength reduces density of the complex while the same treatment at 0,4 M NaCl has no effect; DNA-protein complexes isolated with urea-high salt treatment are visualised as globular particles 25-35 nm in diameter with DNA loops attached. These particles were not observed after detergent treatment although protein composition of the complex remained the same.

Animals↗

Activation of peritoneal macrophages by oil-attached cell-wall skeleton of BCG and Nocardia rubra.

The effect of oil-attached cell-wall skeletons of BCG (BCG-CWS) and Nocardia rubra (N. rubra-CWS) on macrophage functions was examined regarding phagocytosis of zymosan particles, uptake of 2-deoxy-D-glucose (2-dG), and cytostatic activity against syngeneic tumor cells. A single intraperitoneal injection of either BCG-CWS or N. rubra-CWS in mice resulted in accumulation of a large number of macrophages in the peritoneal cavity. They demonstrated, in vitro, a significant cytostatic effect on target cells as well as increase in phagocytic activity and uptake of 2-dG. These results suggest that one of underlying mechanisms for the antitumor activity of BCG-CWS and N. rubra-CWS is possibly the activated macrophage-mediated system.

Animals↗

Induction of a tumor necrosis factor-like activity by Nocardia rubra cell wall skeleton.

Nocardia rubra cell wall skeleton (N-CWS) stimulated adherent cells harvested from the peritoneal cavities of thioglycollate-treated mice to produce cytotoxic activity. Depletion of macrophages from the adherent cells by 2-chloroadenosine or silica abrogated the production of this cytotoxic activity, whereas treatment of the adherent cells with anti-Thy-1.2 antibody and complement did not. This suggested that macrophages were the producer cells of the activity. Cytotoxic activity became detectable as early as 2 h after N-CWS treatment and reached peak activity at 9 h, then declined to a lower level, indicating rapid onset without persistent effects. N-CWS-induced cytotoxic factors have a fairly narrow temperature range, pH optimum for storage, and are sensitive to pronase and trypsin. By using column chromatography, N-CWS-induced cytotoxic factors were compared in detail with tumor necrosis serum obtained from Bacillus Calmette-Guérin endotoxin-treated mice. Both toxins were found to be nearly identical with respect to their behavior in ion-exchange, gel filtration, and concanavalin A affinity columns. N-CWS also induced human peripheral blood lymphocytes to release cytotoxic activity. Monocytes predominantly participated in production of this activity as confirmed by treatment with monoclonal antibody and complement. The cytotoxic activity was completely neutralized by anti-human tumor necrosis factor antiserum, but not by anti-human lymphotoxin antiserum. The fact that human peripheral blood lymphocytes release tumor necrosis-like factors after stimulation with N-CWS might account for the antitumor effects of this agent.

Animals↗

Partial inhibition of the growth of Mycobacterium lepraemurium in C3H mice immunized with cell wall skeletons.

C3H mice stimulated with M. lepraemurium cell wall skeletons (LM-CWS) were challenged with viable M. lepraemurium, and delayed-type hypersensitivity (DTH) to picryl chloride was measured. In one type of experiment the mice were challenged at the time when stimulation of cell-mediated resistance by means of LM-CWS was undertaken. The major purpose was to investigate principles pertaining to immunotherapy. In contrast to the loss of a detectable DTH response to picryl chloride, development of murine leprosy was partially suppressed. In the second type of experiment the mice were stimulated with oil-attached LM-CWS seven weeks before challenge with M. lepraemurium. Findings were: a) that nonspecific DTH as measured by sensitization and challenge with picryl chloride was activated before the infection with M. lepraemurium and b) that the DTH which developed was associated with partial protection against the growth of lepromata. The murine leprosy which developed in C3H mice stimulated with LM-CWS was progressive after some delay.

Animals↗

[Benign and malignant tumors of the thoracic skeleton after percutaneous radiotherapy of Hodgkin's and non-Hodgkin's lymphomas].

Disorders of the skeleton in the thoracic area after percutaneous irradiation of malignant lymphomas are relatively rare. The most common site for pathologic changes is the clavicula. Local overdosage and, in case of malignant alterations, an additional chemotherapy might be the reasons. Among our patients seven lesions occurred, three of them were malignant: two osteogenic sarcomas and one chondrosarcoma.

Adult↗

Properties of interferon induced by purified protein derivative of tuberculin in mice sensitized with BCG or cell-wall skeleton of BCG.

Mice sensitized with either BCG or cell-wall skeleton of BCG (BCG-CWS) produced interferon in blood after stimulation with specific antigen, purified protein derivative of tuberculin (PPD). Both BCG-infected normal (C57BL/6) and thymic nude (BALB/c, nu/nu) mice showed enhanced activity to produce interferon by stimulation with E. coli endotoxin. However, detectable interferon was not produced in athymic nude mice sensitized with BCG or BCG-CWS by stimulation with PPD. Immune-induced interferon (I-IF) produced by BCG-CWS and PPD in mice was different in biological and physicochemical properties from virus-induced interferon. I-IF showed about 100 times more potent L-cell growth inhibitory activity than virus-induced L-cell interferon (L-IF). Both I-IF and L-IF showed macrophage-activating activity, which renders resting macrphages cytotoxic to L1210 leukemia cells. Antiviral and macrophage-activating activity of interferon preparation was not separated physicochemically in this study.

Animals↗