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A challenge to the cross-cultural validity of the SF-36 health survey: factor structure in Maori, Pacific and New Zealand European ethnic groups.

This paper reports on a principal component factor analysis of the SF-36 health status questionnaire in the three major ethnic groups in New Zealand (New Zealand Europeans, Maori and Pacific). The SF-36 is hypothesised to have a two-dimensional structure with distinct (weakly correlated) mental and physical health components, and support for this structural model has generally been found cross-nationally. However, in Maori and Pacific models of health mental and physical dimensions are not generally seen as separable, or independently functioning. This raises the possibility that the questionnaire's hypothesised structural model would not be supported among Maori and Pacific ethnic groups. This study evaluated that possibility. The results of the analysis showed a similar factor structure among New Zealand Europeans, and younger Maori (<45 years) to that reported by Ware et al. for Western European countries. Among Pacific people and older Maori (45 years and over), however, the factor structure did not clearly differentiate physical and mental health components. Implications are discussed both specific to the SF-36 (and in particular the use of principal component summary scores), and more generally for the cross-cultural validity of self-reported health status measures.

Activities of Daily Living↗

Inductively coupled plasma optical emission spectrometric determination of minerals in thyme honeys and their contribution to geographical discrimination.

Twenty-four Spanish thyme honey samples were analyzed using inductively coupled plasma optical emission spectrometry (ICP-OES). Twenty-four minerals were quantified for each honey. The elements Al, As, Ba, Ca, Cr, Cu, Fe, K, Li, Mg, Mn, Na, P, Pb, S, Se, Si, Sr, and Zn were detected in all samples; seven elements are very abundant (Ca, K, Mg, Na, P, S, and Si), and six are not abundant (Al, Cu, Fe, Li, Mn, and Zn). Eleven of them are trace elements (As, Ba, Cd, Co, Cr, Ni, Mo, Pb, Se, Sr, and V) at <1 mg kg(-)(1). Classification of thyme honeys according to their origin (coast, mountains) was achieved by pattern recognition techniques on the mineral data. By means of principal component analysis, a good separation by geographical origin is obtained when scores for the two first principal components are plotted. Classification functions of 11 metals (Al, As, Cr, Cu, K, Li, Mg, Na, P, S, and V) were obtained using stepwise discriminant analysis and applied to classify correctly approximately 100% of the honey samples.

Honey↗

Biological aging of sherry wines using pure cultures of two flor yeast strains under controlled microaeration.

Sherry wines obtained after biological aging for an average of 0, 2, and 4 years were inoculated separately with the flor yeast strains Saccharomyces cerevisiae var. capensis and Saccharomyces bayanus and subjected to short, periodic microaeration to a dissolved oxygen concentration of 4 mg L(-1) after formation of the yeast film. A principal component analysis with the acetaldehyde, ethanol, volatile acidity, and glycerol concentrations obtained was performed. The first principal component was found to account for 49.5% of the overall variance and to be defined mainly by glycerol and ethanol. The second component accounted for 38.8% of the variance and was defined by volatile acidity and acetaldehyde. The conditions used in the tests allowed the biological aging of the wines to be substantially shortened. Thus, 42 days after flor-film formation by S. cerevisiae var. capensis, 0- and 2-year-old wines exhibited parameter values similar to those obtained for the wine aged for 4 years. The wines inoculated with S. bayanus exhibited high acetaldehyde concentrations and ethanol levels above 15% (v/v)-sherry wines with alcohol concentrations below 14.5% are undesirable-, so one need not exclude the sequential or simultaneous inoculation of S. bayanus together with S. cerevisiae var. capensis in order to improve the biological aging process.

Acetaldehyde↗

Matching object size by controlling finger span and hand shape.

The ability of human subjects to accurately control finger span (distance between thumb and one finger) was studied. The experiments were performed without visual feedback of the hand and were designed to study the dependence of accuracy on object size, shape, distance, orientation and finger configuration. The effects of finger combination and sensory modality used to perceive object size (vision and haptics) were also studied. Subjects were quite proficient at this task; the small errors tended to be predominantly negative, i.e., finger span < object size. The thumb-little finger combination was less accurate than the other finger combinations, irrespective of the sensory modality used. Subjects made larger under-estimating errors when matching the size of cylinders than when matching cubes and parallelepipeds. No effect of viewing distance, object orientation and finger configuration was found. Accuracy in matching object size was not dependent on the sensory modality used. The question of how the individual degrees of freedom of the fingers and thumb contributed to the control of finger span was also addressed. Principal components analysis showed that two components could characterize the hand postures used, irrespective of object size. The amplitude of the first principal component was constant, and the amplitude of the second scaled linearly with object size. This finding suggests that all of the degrees of freedom of the hand are controlled as a unit. This result is discussed in relation to the 'virtual finger' hypothesis for grasping.

Adult↗

Phenotypic responses of wild barley to experimentally imposed water stress.

Responses to water stress within a population of wild barley from Tabigha, Israel, were examined. The population's distribution spans two soil types: Terra Rossa (TR) and Basalt (B). Seeds were collected from plants along a 100 m transect; 24 genotypes were sampled from TR and 28 from B. Due to different soil water-holding capacities, plants growing on TR naturally experience more intense drought than plants growing on B. In a glasshouse experiment, water was withheld from plants for two periods (10 d and 14 d) after flag leaf emergence. A total of 15 agronomic, morphological, developmental, and fertility related traits were examined by analysis of variance (ANOVA). Ten of these traits were significantly affected by the treatment. A high degree of phenotypic variation was found in the population with significant genotypextreatment and soil typextreatment interactions. Principal component analysis (PCA) was performed using combined control and stress treatment data sets. The first three principal components (pc) explained 88.8% of the variation existing in the population with pc1 (47.9%) comprising yield-related and morphological traits, pc2 (22.9%) developmental characteristics and pc3 (18.0%) fertility-related traits. The relative performance of individual genotypes was determined and water stress tolerant genotypes identified. TR genotypes were significantly less affected by the imposed water stress than B genotypes. Moreover, TR genotypes showed accelerated development under water deficit conditions. Data indicate that specific genotypes demonstrating differential responses may be useful for comparative physiological studies, and that TR genotypes exhibiting yield stability may have value for breeding barley better adapted to drought.

Adaptation, Physiological↗

Geographical variation of mandible size and shape in the Japanese wild pig (Sus scrofa leucomystax).

The mandibles of the Japanese wild pigs (Sus scrofa leucomystax) from various geographical locations, which have been stored in the University of Tokyo and National Science Museum, Tokyo were examined. The mandibles from northern localities were larger in size than those from southern localities. The Oita population was significantly smaller than the Honshu populations. The Mie population was smaller than the Hyogo population in length from the angle of the mandible. In the multivariate analysis, the Kyushu populations could be obviously distinguished from the Honshu populations in the principal component charts. However, each Honshu population did not show the locality-specific distribution of the principal component plots.

Animals↗

Individual differences of the contribution of chromatic channels to brightness.

Perceived brightness is considered to be a combined consequence of outputs of the luminance channel and the chromatic channels in the visual system. The differences of logarithmic spectral luminous efficiencies between heterochromatic brightness matching and flicker photometry that were obtained from 16 subjects were examined by using principal component analysis. The luminous-efficiency difference between the two methods is described by only two principal components. Individual characteristics of the contribution of chromatic channels to brightness can be specified by measuring luminous efficiencies at 470 and 660 nm.

Adult↗

Polymorphism of Ag(+)-NORs in cervical smears from women with cervical cancer.

OBJECTIVE: To evaluate Ag(+)-stained (Ag(+)-NOR) polymorphism in four groups of patients with various grades of cervical lesions and in a control group. STUDY DESIGN: Forty-five women were selected, diagnosed and classified on the bases of the Pap smear and colposcopy/biopsy at Hospital de Ginecologia y Obstetricia del Instituto Mexicano del Seguro Social in Monterrey, Mexico. Five categories were considered: (1) inflammatory, (2) low grade squamous intraepithelial lesions (LSILs), (3) high grade squamous intraepithelial lesions (HSILs), (4) invasive cervical cancer, and (5) normal. The cervical smears were stained by the Ag(+)-NOR method. One hundred cells per slide were counted and classified according to the polymorphism of Ag(+)-NOR dots: typical (spherical) and atypical (large, kidney shaped and clustered). The four shapes of Ag(+)-NORs were quantified by percentage and transformed using the arcsine root procedure. RESULTS: Statistical analysis showed a significant decrease in spherical shape according to neoplastic development. The three atypical shapes showed a significant increase in patients with HSIL and invasive carcinoma in respect to LSIL. Principal components analysis grouped the data at five locations in the plane formed by the first two principal components according to the diagnosis. CONCLUSION: These findings suggest the potential diagnostic and prognostic value of the determination of Ag(+)-NOR polymorphism in cervical cytology studies.

Analysis of Variance↗

Partial Cox regression analysis for high-dimensional microarray gene expression data.

MOTIVATION: An important application of microarray technology is to predict various clinical phenotypes based on the gene expression profile. Success has been demonstrated in molecular classification of cancer in which different types of cancer serve as categorical outcome variable. However, there has been less research in linking gene expression profile to censored survival outcome such as patients' overall survival time or time to cancer relapse. In this paper, we develop a partial Cox regression method for constructing mutually uncorrelated components based on microarray gene expression data for predicting the survival of future patients. RESULTS: The proposed partial Cox regression method involves constructing predictive components by repeated least square fitting of residuals and Cox regression fitting. The key difference from the standard principal components of Cox regression analysis is that in constructing the predictive components, our method utilizes the observed survival/censoring information. We also propose to apply the time-dependent receiver operating characteristic curve analysis to evaluate the results. We applied our methods to a publicly available dataset of diffuse large B-cell lymphoma. The outcomes indicated that combining the partial Cox regression method with principal components analysis results in parsimonious model with fewer components and better predictive performance. We conclude that the proposed partial Cox regression method can be very useful in building a parsimonious predictive model that can accurately predict the survival of future patients based on the gene expression profile and survival times of previous patients. AVAILABILITY: R codes are available upon request.

Biomarkers, Tumor↗

Impairment and disability in patients with facial neuromuscular dysfunction.

Integration of impairment measures and disability measures may provide clinicians with an accurate and comprehensive picture of the patient's dysfunction. The purpose of this study was to indicate the usefulness of two new scales of measuring facial impairment and disability in describing characteristics of individuals with facial neuromuscular dysfunction. Fifty-one individuals with unilateral facial neuromuscular dysfunction and a House-Brackmann grade of III or higher were included in the study. The subjects' movement impairments were assessed using the Facial Grading System (FGS). The subjects reported their physical and social function on the Facial Disability Index (FDI). Nine variables were subjected to a confirmatory principal-components factor analysis to indicate important factors in describing patients with facial nerve disorders. The confirmatory principal-components factor analysis identified three factors, impairment, disability, and temporal characteristics of disease, accounting for 72% of the variance in describing individuals with facial neuromuscular dysfunction. Integration of these measures may provide clinicians with an accurate and comprehensive picture of the patient's dysfunction, thus aiding in the determination of intervention and the measurement of clinical outcomes.

Adult↗

Chemical discrimination of arabica and robusta coffees by Fourier transform Raman spectroscopy.

This article deals with the potential of Fourier transform (FT) Raman spectroscopy in discrimination of botanical species of green and roasted coffees. There are two species of commercial importance: Coffea arabica (arabica) and Coffea canephora (robusta). It is recognized that they differ in their lipid fraction, especially in the content of the diterpene kahweol, which is present at 0.1-0.3% dry matter basis in arabica beans and only in traces (<0.01%) in robusta. The visual examination of the Raman spectra of the lipid fraction extracted from arabica, robusta and liberica samples shows differences in the mid-wavenumbers region: arabica spectra have two characteristic scattering bands at 1567 and 1478 cm(-1). The spectrum of the pure kahweol shows the same bands. Principal component analysis is applied to the spectra and reveals clustering according to the coffee species. The first principal component (PC1) explains 93% of the spectral variation and corresponds to the kahweol concentration. Using the PC1 score plot, two groups of arabica can be distinguished as follows: one group with high kahweol content and another group with low kahweol content. The first group includes samples coming from Kenya and Jamaica; the second group includes samples from Australia. The main difference between these coffees is that those from Kenya and Jamaica are well-known for growing at a high altitude whereas those ones from Australia are grown at a low altitude. To our knowledge, the application of Raman spectroscopy has never been used in coffee analysis.

Altitude↗

Social desirability and Wiggin's MMPI content scales.

Using the Minnesota Multiphasic Personality Inventory (MMPI) item pool, Wiggins (1966) developed 13 scales, each with a homogeneous content. The 13 scales, along with marker scales for the 1st 3 MMPI factors, Edward's social desirability (SD), Welsh's repression (R), and Wiggins's social desirability (Sd) respectively were scored in the MMPI. The same scales were scored in an Experimental Multiphasic Personality Inventory (EMPI). A principal-components analysis of the 16 scales when scored in the MMPI resulted in 4 factors. A principal-components analysis of these same scales when scored in the MMPI and when scored in the EMPI were found to be highly congruent. The SD, R, and Sd scales proved to be excellent markers for the 1st 3 factors of the MMPI and also for the 1st 3 factors of the EMPI. Results provide further evidence that the 1st MMPI factor is a social desirability factor rather than a content factor.

Adult↗

Source characterization of atmospheric heavy metals in industrial/residential areas: a case study in Oman.

Total suspended particulate matter (TSP) was collected from two districts: the Sohar industrial estate (SIE) and a residential area in the case study area in Oman. The TSP collected from SIE was taken from 19 different industrial activities, whereas those collected from the residential area were taken from 12 houses. The samples were analyzed for nine heavy metals: chromium (Cr), copper (Cu), manganese (Mn), nickel, lead (Pb), zinc (Zn), cadmium, vanadium, and molybdenum. The results were used to assess the source classes responsible for TSP and heavy metal levels in the atmosphere of the two districts. At each district, principal component analysis was applied to the concentrations of TSP and heavy metals to obtain the number of principal components. At SIE, three groups of elements were determined. The first component contained large factor loadings in Cu and Mn (Cu industrial processes and reinforcement steel production). The second presented most of the variance of Cr, Pb, and Zn (black and galvanized iron pipes production, mechanical industries, and vehicle construction). The third component was composed of Zn and Pb that probably reflected contributions from motor-vehicle tire wear or was associated with municipal incineration. On the other hand, results from the village showed that only one factor was able to explain the main part of the data variance, and the industrial site was polluting air quality in the village with Cu, Cr, Pb, nickel, and Mn.

Air Pollutants↗

Audiological correlates of speech understanding deficits in elderly listeners with mild-to-moderate hearing loss. III. Factor representation.

OBJECTIVE: The objective of the study was to determine the major factors that underlie auditory/audiological performance measures in an elderly population, with particular emphasis on finding those factors responsible for speech understanding under specific conditions of interference. DESIGN: Audiological status and auditory performance of a group of elderly (60- to 81-yr-old) and normal-hearing young (18- to 30-yr-old) individuals was determined through a test battery. When present, the hearing loss of elderly subjects was symmetrical in the two ears and, at most, moderate. The battery included tests of speech intelligibility on the word and sentence levels, with and without the presence of interfering speech. In addition to pure-tone and speech reception thresholds, perception of spectrally or temporally distorted speech as well as auditory resolution of frequency, time, and space were tested. Two tests received special consideration: the Speech Perception In Noise Test and the Modified Rhyme Reverberation Test. Taking the overall results as well as various subsets of the results, principal component analyses were conducted to identify major factors underlying auditory performance. RESULTS: The factors extracted by the principal component analyses present a portrayal of the auditory performance profile in which effects of interference, high-frequency hearing, and basic auditory function play a major role. Interference factors include general susceptibility to noise as well as segregation of concurrent speech sounds on the basis of temporal dissimilarities and spatial separation. Comparison of factors extracted from various subsets of tests indicate that factors underlying the decline of the "cocktail party effect" in the elderly are addressed mostly by tests specifically designed to assess speech understanding in spatially distributed babble or in a reverberant environment. CONCLUSIONS: Factor analysis of test measures obtained from a group of elderly individuals with normal hearing or mild-to-moderate hearing loss led to two main findings. First, it portrayed hearing loss as a component of different factors rather than as a factor on its own. Second, the independence of measures of speech understanding in babble or reverberation from other measures suggests that such tests should become an integral part of audiological test batteries designed to assess auditory functions in aging.

Adolescent↗

Bacterial vaginosis: risk factors among Kenyan women and their male partners.

OBJECTIVES: To simultaneously examine associations of bacterial vaginosis (BV) with potential risk factors in both the female and her male partner. STUDY DESIGN: We recruited women 18-45 years of age and their male partners from clinics in Nairobi, Kenya. All underwent face-to-face standardized interview physical examination, human immunodeficiency virus (HIV)-1 and syphilis serologic testing, endocervical cultures for Neisseria gonorrhoeae, and vaginal swabs for diagnosis of BV by Gram stain and trichomoniasis by culture. RESULTS: Of 219 women, 97 (44%) had BV. BV was significantly associated by univariate analyses with women's own risk factors (young age, being unmarried, early sexual debut, more than 1 sexual partner, lifetime, rectal sex, trichomoniasis, HIV infection, and by principal components analysis, with low socioeconomic status [SES]) and also with male partners' characteristics (HIV infection, and by principal components analysis, low SES, and poor hygiene). In multivariate analysis including risk factors from both genders, the odds of having BV was 5.7 times higher if either partner was HIV seropositive, 13.2 times higher if the female had trichomoniasis, 2.5 times higher if the female had more than 1 sex partner ever, and decreased with increasing age of the female. CONCLUSION: In this population, characteristics of males and of females were independently associated with BV. Close association of male hygiene and male HIV status precluded distinguishing the influence of male hygiene on partner's BV.

Adolescent↗

A backpropagation network model of the monaural localization information available in the bat echolocation system.

The information echolocating bats receive is a combination of the properties of the sound they emit and the sound they receive at the eardrum. Convolving the emission and the external ear transfer functions produces the full spectral information contained in the echolocation combination. Spatially dependent changes in the magnitude spectra of the emission, external ear transfer functions, and the echolocation combination of Eptesicus fuscus could provide localization information to the bat. Principal component analysis was used to reduce the dimensionality of these complex spectral data sets. The first eight principal component weights were normalized, rotated, and used as the input to a backpropagation network model which examined the relative directionality of the emission, ear, and the echolocation combination. The model was able to localize more accurately when provided with the directional information of the echolocation combination compared to either the emission or ear information alone.

Animals↗

Parallel distributed network characteristics of the DSCT.

1. We examined the functional organization of the dorsal spinocerebellar tract (DSCT) and found that it is similar to that of a parallel distributed network having widespread connectivity among parallel elements. The prevailing view is that the DSCT provides receptor-specific information to the cerebellum regarding muscle and cutaneous inputs from the hindlimbs, but that view does not consider the convergent inputs to DSCT neurons from multimodal polysynaptic pathways. 2. Spontaneously active DSCT neurons respond to peripheral stimulation with changes in their firing probability. We characterized the temporal patterns of poststimulus excitability changes for a large number of neurons using principal component analysis. The response of each neuron was represented by a response vector in three-dimensional principal component space, in which similar vectors represent responses having a similar waveform for their poststimulus activity patterns. 3. We compared the responses of large populations of DSCT units to two types of stimuli: small (3-8 deg) passive rotations of the foot at the ankle of an intact limb (234 cells) and stretch or contraction of an isolated muscle group (gastrocnemius-soleus, 168 cells). Most of the cells tested had significant responses (P < 0.05) to both types of stimuli (40-78% responded to muscle stimulation and 88% to foot rotation), and they exhibited similar patterns of poststimulus activity. Long-lasting inhibitory responses and excitatory responses with a range of peak times (< 10- > 60 ms) were prevalent in all cases. The population response to each stimulus was characterized by the relative incidence of response types among the units in a representative sample of the population. 4. The time course of excitability changes induced in DSCT cells by the stimuli could have been determined primarily by the presynaptic circuitry or by postsynaptic factors intrinsic to the DSCT cells. The evidence presented suggests that the selection of response waveforms and their distribution among the DSCT cells was determined presynaptically. We found that individual cells were capable of diverse responses to different stimuli. 5. Sample groups of 7-30 cells were selected at random and also on the basis of the similarity of their responses to one type of stimulus. The distributions of response types among the cells of the sample groups were compared to the distributions for the entire population recorded for each stimulus.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗

Identification of Macrosomic, normal and intrauterine growth retarded neonates using the modified Neonatal Growth Assessment Score.

OBJECTIVE: Separation of Macrosomic, normal and intrauterine growth retarded (IUGR) neonates using the modified Neonatal Growth Assessment Score (NGAS). METHODS: A sample of 23 neonates with evidence of growth acceleration and 74 neonates classified as normal or IUGR in a previous study was used in this investigation. The prenatal growth of all neonates had been studied longitudinally with ultrasound. From the data collected, Rossavik models describing the growth trajectories of head circumference (HC), abdominal circumference (AC), thigh circumference (ThC), femur diaphysis length, head cube (A) and abdominal cube (B) were specified. These models were used to predict birth weight (WT), crown-heel length (CHL), HC, AC and ThC at birth. Actual birth measurements of WT, CHL, HC, AC and ThC were evaluated using age-specific normal size curves and compared to predicted measurements by means of the growth potential realization index (GPRI). GPRI values were evaluated by comparison to previously established normal ranges and used to calculate Neonatal Growth Assessment Scores (NGAS(5)). NGAS(5) values, together with assessments of anatomical measurements and GPRI values, were used to establish macrosomic, normal and IUGR groups. Principal components analysis was applied to the sets of GPRI values available for each neonate to provide a principal component score for separating macrosomic and normal neonates (m(2)NGAS(51)) or one to separate macrosomic, normal and IUGR neonates (m(3)NGAS(51)) using linear discriminant analysis. The groups identified by these multivariate methods were compared to the original classification and their characteristics evaluated. RESULTS: The m(2)NGAS(51) and a boundary value of 207.5% separated macrosomic and normal neonates with an accuracy of 97.3%. The m(3)NGAS(51) and boundary values of 210.0 and 182.5% separated macrosomic, normal and IUGR neonates with an accuracy of 96.9%. No single GPRI value or anatomical measurement could achieve these levels of accuracy. All normal infants were AGA but only 45.5% of the IUGR group were SGA and only 60.9% of the macrosomic group were LGA. Thirteen different types of IUGR and eleven different types of macrosomia were identified based on GPRI values. CONCLUSION: The modified NGAS accurately separates macrosomic, normal and IUGR neonates although growth abnormalities are expressed in different ways in different individuals.

Adult↗