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[Studies on arsenic metabolism (XX). Arsenic accumulation in the organs and excretion into the feces and urine of rats chronically poisoned with arsenic (author's transl)].

Male and female rats (60 approximately 80 g) of Wistar strain were randomly divided into two groups and were given milk and cereal diets, respectively. Each group was further divided into two groups; one was given the diet containing 100ppm of arsenic trioxide and the other a diet containing "arsenic compound" (100ppm as arsenic trioxide). Each group included five rats of both sexes. A 6-month feeding of the test diet was followed by provision of a normal diet. The accumulated arsenic was excreted almost 100% from the brain and 20 approximately 30% from organs such as kidney, liver, spleen and lung. The arsenic level persisted in tissues in animals on the cereal diet, as compared with those fed the milk diet. There was no significant difference in the accumulation and excretion of arsenic between the groups given arsenic trioxide or "arsenic compound".

Animals↗

Effects of germinated barley foodstuff in preventing diarrhea and forming normal feces in ceco-colectomized rats.

Germinated barley foodstuff (GBF) derived from the aleurone and scutellum fractions of germinated barley was rich in glutamine and low-lignified hemicellulose. The diarrhea caused by ceco-colectomy could be prevented by feeding GBF to rats. GBF could also increase the protein content and sucrase activity of small intestinal mucosa in this model. This diarrhea-preventive effect of GBF would be based on the water-holding capacity and bulging force under alkaline conditions, e.g. in the small intestine.

Animals↗

Serotype 2 reoviruses from the feces of cats with and without diarrhea.

During a virus survey carried out in the period 1989-90 with 148 fecal samples collected from cats in Japan, three reovirus strains were isolated in feline cell cultures. Two strains (Nos. 114 and 140) were from 48 diarrheal fecal samples and another strain (No. 32/41) was from 100 normal fecal samples. The strains grew in feline and simian cell cultures with producing typical intracytoplasmic inclusion bodies in which virus particles were densely packed. All strains, especially Nos. 32/41 and 140 strains, showed trypsin-dependent growth in vitro. Their ultrastructural and genomic properties were characteristic of genus reovirus in the Reoviridae. All strains agglutinated erythrocytes of human type O but not of bovine. Although they were identified as serotype 2 by hemagglutination-inhibition test with the hyperimmune sera against human reovirus prototype strains, No. 114 strain was typical and the other two strains were atypical serotype 2 reoviruses. Furthermore, from the reason that Nos. 32/41 and 140 strains possessed some common properties though derived from cats in distant locations, they were considered to be reoviruses having been maintained in the cat population. Seroepizootiologic survey revealed that the prevalence of serotype 3 infection was most widespread and serotype 2 was least among three serotypes of reovirus in a cat population.

Animals↗

Experimental infection of pigs with a parvovirus isolated from the diarrheic feces of a pig.

A parvovirus (the H-45 strain) isolated from an outbreak of epizootic diarrhea in swine was examined to observe the infectivity and pathogenecity in swine. The virus infection by intranasal route was demonstrated in each group of 2- and 5-day-old colostrum-deprived pigs, 30- and 100-day-old pigs by virus recovery from the nasal and rectal swabs, and detecting seroconversion. The virus was recovered from rectal swabs up to 14 days after inoculation and from nasal swabs up to 9 days. Uninoculated pigs were infected with the virus by contacting with the inoculated pigs. Between 1 and 5 days after inoculation, the inoculated pigs of 2, 5 and 30 days old developed diarrhea and then all the pigs of 2 days old died, resulting from dehydration. In the pigs died after intranasal inoculation, the virus was recovered mainly from the respiratory and gastrointestinal tracts. One hundred-day-old pigs showed no clinical signs. The pathological change was characterised by congestion and edema with hemorrhage in the epithelium of the small intestines exhibiting additional degeneration and desquamation of the epithelial cells.

Aging↗

Isolation of reovirus type 2 from diarrheal feces of pigs.

Reoviruses designated as OS-320 to OS-324 were isolated from a total of 5 fecal specimens from 3 with diarrhea and 2 apparently healthy pigs aged 3 months. The serotype of these isolates was determined as reovirus type 2 by cross neutralizing tests. Furthermore, the results of hemagglutination test suggested the isolates were different from the other reoviruses. A survey of neutralizing antibody against reoviruses showed type 2 virus was widely prevalent among pigs in Okayama Prefecture. This paper is the first report of the isolation of reovirus type 2 from pigs.

Animals↗

Isolation of Rhodococcus equi from the feces of indigenous animals and soil from the Lower Zambezi National Park and Lochinvar National Park, Zambia.

Rhodococcus equi is an important pathogen in foals; however, its incidence in African indigenous animals is poorly understood. Fecal samples (92 from nine indigenous species) and 43 soil samples were collected from two Zambian National Parks. The presence of R. equi was investigated and 533 isolates were tested for the presence of 15- to 17-kDa antigens (VapA) and a 20-kDa antigen (VapB) by immunoblotting and PCR. R. equi was isolated (10(2)-10(4) colony forming units/g) from 75% of fecal and 74% of soil samples. Neither antigen was detected; however, about 20% of the isolates contained cryptic plasmids of various sizes. There was no evidence of virulent R. equi, but the avirulent form was widespread in the animals and the soil.

Animals↗

Survey of coccidial oocysts and parasite eggs in feces of free-ranging Grus japonensis.

Fecal survey of Eimeria oocysts and parasite eggs was conducted for 219 fecal samples of free-ranging Grus japonensis in Kushiro district in Hokkaido in April 2003. Positive rate and mean oocysts (or eggs) per gram in positive samples were 26% (57/219) and 8.8 (0.2-136) in oocysts of Eimeria reichenowi, 18.3% (40/219) and 320 (100-1,000) in trematode eggs, 0.1% (2/219) and 0.2 (0.2-0.3) in eggs of Nematoda A, and 4.1% (9/219) and 0.8 (0.2-3.6) in eggs of Nematoda B, respectively.

Animals↗