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Performance of a flat-panel detector in the detection of artificial erosive changes: comparison with conventional screen-film and storage-phosphor radiography.

The purpose of this study was to compare a large-area, direct-readout, flat-panel detector system with a conventional screen-film system, a storage-phosphor system, and a mammography screen-film system with regard to the detection of artificial bone erosions simulating rheumatoid disease, and to assess its diagnostic performance with decreasing exposure dose. Six hundred forty regions were defined in 160 metacarpophalangeal and proximal interphalangeal joint specimens from 20 monkey paws (4 regions per joint). Artificial bone erosions were created in 320 of these 640 regions. Specimens were enclosed in containers filled with water to obtain absorption and scatter radiation conditions similar to those of a human hand. Imaging was performed using a flat-panel system, a speed class 200 screen-film system, a mammography screen-film system, and a storage-phosphor system under exactly matched conditions. Different exposure doses equivalent to speed classes of S=100, 200, 400, 800, 1600, and 3200 were used. In all images the presence or absence of a lesion was assessed by three radiologists using a five-level confidence scale. Receiver operating characteristic (ROC) analysis was performed for a total of 21,120 observations (1920 for each imaging modality and exposure level) and diagnostic performance estimated by the area under the ROC curve (A(z)). The significance of differences in diagnostic performance was tested with analysis of variance. The ROC analysis showed A(z) values of 0.809 (S=200), 0.768 (S=400), 0.737 (S=800), 0.710 (S=1600), and 0.685 (S=3200) for the flat-panel system, 0.770 for the speed class 200 screen-film system, 0.781 (S=200), 0.739 (S=400), 0.724 (S=800), 0.680 (S=1600) for the storage-phosphor system, and 0.798 for the mammography screen-film system. Analysis of variance showed significant differences between different combinations of imaging modalities and exposure doses ( p<0.05). The diagnostic performance of the flat-panel detector system is superior to that of a screen-film system and a storage-phosphor system for the detection of erosive lesions at clinical exposure settings (S=200). Using the flat-panel system the exposure dose can be reduced by 50% to obtain a diagnostic performance comparable to a speed class 200 screen-film system.

Animals↗

Hysterectomized women with ovarian conservation report more severe climacteric complaints than do normal climacteric women of similar age.

OBJECTIVE: Our purpose was to compare the severity of typical climacteric complaints (vasomotor complaints and vaginal dryness) and 21 other complaints, considered atypical for the climacteric, in women with and without a uterus. STUDY DESIGN: A cross-sectional population questionnaire survey was sent to all women aged 39 through 60 years in Ede, the Netherlands. Subjects were 986 hysterectomized women (one or both ovaries present) and 5636 normal women (uterus and both ovaries present). Statistical analysis was performed by cross tabulations, chi 2 analysis, analysis of variance, meta-analysis of variance, and ratios. RESULTS: Hysterectomized women, especially those aged 39 to 41 years, report significantly more vasomotor complaints, vaginal dryness, and atypical complaints than do normal climacteric women of the same age. The higher prevalence of typical climacteric complaints in hysterectomized women largely explains their higher level of atypical complaints. CONCLUSION: Physicians should be alert to typical climacteric complaints after hysterectomy with ovarian conservation, especially in young women, because the literature indicates that hysterectomized women with ovarian conservation are overrepresented with regard to osteoporosis, cardiovascular disease, osteoarthritis, depression, and sexual problems.

Adult↗

Family reactions during infants' hospitalization in the neonatal intensive care unit.

The purpose of this investigation is to assess family stress, coping, perceptions of their infant, and alterations in mood that may result from the hospitalization of their critically ill newborn infant. Eligible patients were those infants hospitalized in the Special Care Nursery (SCN) at Christiana Care Health Services, who were born up to 31 weeks' gestational age. Twenty-seven families (mothers and/or fathers) completed four questionnaires at 2-week intervals during the course of their premature infants' hospitalization. Data were primarily evaluated by using analysis of variance (ANOVA)/multivariate analysis of variance (MANOVA). A score for neonatal acute physiology (SNAP) was obtained in each infant to assess the effect of the severity of neonatal illness on the questionnaire variables. Families with high stress scores on the Parental Stressor Scale had different coping strategies than those with less stress scores. A high level of maternal depressive symptomatology was associated with altered methods of coping, general stress, and perception of infant health. There was no relationship between the SNAP score on the overall level of stress families. Families who completed more than two questionnaires differed from those who only completed two or less questionnaires, although the sample size was too small to assess longitudinal changes in this study population. Level of stress and depressive symptoms are two major influences of how families cope with the current hospitalization of a premature infant. The degree of neonatal illness is not a major contributor to the parents' coping ability. Healthcare providers need to understand these dynamics when supporting families during the hospitalization of their premature infant.

Adaptation, Psychological↗

Solving the protein sequence metric problem.

Biological sequences are composed of long strings of alphabetic letters rather than arrays of numerical values. Lack of a natural underlying metric for comparing such alphabetic data significantly inhibits sophisticated statistical analyses of sequences, modeling structural and functional aspects of proteins, and related problems. Herein, we use multivariate statistical analyses on almost 500 amino acid attributes to produce a small set of highly interpretable numeric patterns of amino acid variability. These high-dimensional attribute data are summarized by five multidimensional patterns of attribute covariation that reflect polarity, secondary structure, molecular volume, codon diversity, and electrostatic charge. Numerical scores for each amino acid then transform amino acid sequences for statistical analyses. Relationships between transformed data and amino acid substitution matrices show significant associations for polarity and codon diversity scores. Transformed alphabetic data are used in analysis of variance and discriminant analysis to study DNA binding in the basic helix-loop-helix proteins. The transformed scores offer a general solution for analyzing a wide variety of sequence analysis problems.

Amino Acid Sequence↗

Ambulatory care education: how do academic medical centers, affiliated residency teaching sites, and community-based practices compare?

PURPOSE: Little is known about how different ambulatory sites compare as clinical educational settings. The authors used students' log data to compare the educational content and processes in academic medical center-based clinics (AMCs), affiliated residency teaching sites (ARTs), and local community-based practices (CBPs) at one medical school. METHOD: Students recorded their experiences with symptoms, counseling, procedures, and common medical conditions as well as characteristics of the learning process during a required eight-week third-year ambulatory clerkship in family medicine. Descriptive statistics, chi-square for differences in categorical variables, and analysis of variance and multivariate analysis of variance for differences in continuous variables were used to compare the educational settings. RESULTS: Over 9,000 encounters were analyzed; 29.7% occurred in AMCs, 14.8% in ARTs, and 55.5% in CBPs. The proportion of continuity visits was lowest in the AMCs and highest in CBPs (13% versus 22%, respectively; p <.01). Students saw almost 57,000 symptoms and conditions. Of 19 symptoms compared, seven differed significantly among the three settings: back pain, cough, dyspnea, ENT (ears, nose, throat problems), fatigue, knee pain, and vomiting. All but one of these were least likely to occur in ARTs. Procedures were performed more frequently, whereas counseling skills were called upon less frequently in CBPs. Students reported being more likely to work unobserved while conducting physical examinations in ARTs and CBPs. Amount of feedback provided on clinical skills did not differ, but students reported receiving more teaching about patient management in ARTs and AMCs versus CBPs (74%, 72%, and 66% of encounters, respectively; p <.01). CONCLUSIONS: Academic and community settings can complement each other as learning sites for an ambulatory clerkship in family medicine, and common curricular expectations can be achieved. Settings' differences and similarities should be taken into account when developing, implementing, or revising clerkship programs and should be considered with students' interests and previous clinical experiences before assigning students to a teaching site.

Academic Medical Centers↗

Detection of subtle undisplaced rib fractures in a porcine model: radiation dose requirement--digital flat-panel versus screen-film and storage-phosphor systems.

PURPOSE: To compare a large-area direct read-out flat-panel detector radiography system with screen-film and storage-phosphor systems with regard to detection of subtle undisplaced rib fractures and to assess the diagnostic performance of the flat-panel system with decreasing exposure level. MATERIALS AND METHODS: Subtle fractures were created artificially in 100 of 200 porcine rib specimens. Specimens were enclosed in containers of water to generate absorption and scatter radiation conditions similar to those of a human chest wall. Imaging was performed with flat-panel, screen-film, and storage-phosphor systems with conditions that were exactly matched. Different exposure levels equivalent to speed classes (S) of 400, 800, 1,600, and 6,400 were used. All images were independently assessed for the presence of fracture by three radiologists with a five-level confidence scale. Receiver operating characteristic (ROC) analysis was performed for a total of 4,200 observations (600 for each imaging system and exposure level). Diagnostic performance was estimated with area under the ROC curve (Az). Significance of differences in diagnostic performance was tested with analysis of variance. RESULTS: ROC analysis yielded mean Az values for the flat-panel system of 0.879 (S = 400), 0.833 (S = 800), 0.765 (S = 1,600), and 0.576 (S = 6,400). Az values were 0.834 (S = 400) for the screen-film system and 0.789 (S = 400) and 0.729 (S = 800) for the storage-phosphor system. Analysis of variance revealed significant differences in diagnostic performance between various combinations of imaging system and exposure levels (P <.05). CONCLUSION: The flat-panel system is superior to the screen-film and storage-phosphor systems for detection of subtle undisplaced rib fractures at clinical exposure settings (eg, S = 400). With the flat-panel system, radiation dose can be reduced by 50% to achieve diagnostic performance comparable to that of a speed class 400 screen-film system.

Animals↗

Normal spinal bone marrow in adults: dynamic gadolinium-enhanced MR imaging.

PURPOSE: To determine the patterns of dynamic enhancement of normal spinal bone marrow in adults at gadolinium-enhanced magnetic resonance (MR) imaging and the changes that occur with aging. MATERIALS AND METHODS: Dynamic contrast material-enhanced MR imaging of the thoracolumbar spine was performed in 71 patients. The maximum percentage of enhancement (Emax), enhancement slope, and enhancement washout were determined from bone marrow enhancement time curves (ETCs). The bone marrow signal intensity on T1-weighted spin-echo MR images was qualitatively classified into three grade categories. Quantitative ETC values were correlated with patient age and bone marrow fat content grade. Statistical analysis included mean t test comparison, analysis of variance, and regression analysis of the correlations between age and quantitative MR parameters. RESULTS: Emax, slope, and washout varied widely among the patients. Emax values were obtained within 1 minute after contrast material injection and ranged from 0% to 430%. Emax values were significantly higher in patients younger than 40 years than in those aged 40 years or older (P <.001). These values decreased with increasing age in a logarithmic relationship (r = 0.71). Emax values decreased as fat content increased, but some overlap among the fat content grades was noted. Analysis of variance revealed that Emax was significantly related to age (younger than 40 years vs 40 years or older) (P <.001) and fat content grade (P <.001) but not significantly related to sex. CONCLUSION: Dynamic contrast-enhanced MR imaging patterns of normal spinal bone marrow are dependent mainly on patient age and fat content.

Adolescent↗

Effects of training and a single session of exercise on lipids and apolipoproteins in hypercholesterolemic men.

To differentiate between transient (acute) and training (chronic) effects of exercise at two different intensities on blood lipids and apolipoproteins (apo), 26 hypercholesterolemic men (cholesterol = 258 mg/dl, age = 47 yr, weight = 81.9 kg) trained three times per week for 24 wk, 350 kcal/session at high (80% maximal O2 uptake, n = 12) or moderate (50% maximal O2 uptake, n = 14) intensity. Serum lipid and apolipoprotein (apo) concentrations (plasma volume adjusted) were measured before and immediately, 24, and 48 h after exercise on four different occasions corresponding to 0, 8, 16, and 24 wk of training. Data were analyzed using three-way repeated-measures multivariate analysis of variance followed by analysis of variance and Duncan's procedures (alpha = 0.05). A transient 6% rise in low-density-lipoprotein cholesterol measured before training at the 24-h time point was no longer evident after training. Triglycerides fell and total cholesterol, high-density-lipoprotein cholesterol (HDL-C), HDL3-C, apo A-I, and apo B rose 24-48 h after exercise regardless of training or intensity. Total cholesterol, HDL3-C, apo A-I, and apo B were lower and HDL2-C was higher after training than before training. Thus exercise training and a single session of exercise exert distinct and interactive effects on lipids and apolipoproteins. These results support the practice of training at least every other day to obtain optimal exercise benefits.

Adult↗

The Brookline Early Education Project: a 25-year follow-up study of a family-centered early health and development intervention.

BACKGROUND: Clinicians, scientists, and policy makers are increasingly taking interest in the long-term outcomes of early intervention programs undertaken during the 1960s and 1970s, which were intended to improve young children's health and educational prospects. The Brookline Early Education Project (BEEP) was an innovative, community-based program that provided health and developmental services for children and their families from 3 months before birth until entry into kindergarten. It was open to all families in the town of Brookline and to families from neighboring Boston, to include a mixture of families from suburban and urban communities. The goal of the project, which was administered by the Brookline Public Schools, was to ensure that children would enter kindergarten healthy and ready to learn. OBJECTIVE: Outcome studies of BEEP and comparison children during kindergarten and second grade demonstrated the program's effectiveness during the early school years. The goal of this follow-up study was to test the hypotheses that BEEP participants, in comparison with their peers, would have higher levels of educational attainment, higher incomes, and more positive health behaviors, mental health, and health efficacy during the young adult period. METHODS: Participants were young adults who were enrolled in the BEEP project from 1973 to 1978. Comparison subjects were young adults in Boston and Brookline who did not participate in BEEP but were matched to the BEEP group with respect to age, ethnicity, mother's educational level, and neighborhood (during youth). A total of 169 children were enrolled originally in BEEP and monitored through second grade. The follow-up sample included a total of 120 young adults who had participated in BEEP as children. The sample differed from the original BEEP sample in having a slightly larger proportion of college-educated mothers and a slightly smaller proportion of urban families but otherwise resembled the original BEEP sample. The demographic features of the BEEP and comparison samples were similar. The young adults were asked to complete a survey that focused on the major domains of educational/functional outcomes and health/well-being. The study used a quasi-experimental causal-comparative design involving quantitative analyses of differences between the BEEP program and comparison groups, stratified according to community. Hypotheses were tested with analysis of variance and multivariate analysis of variance techniques. Analyses of the hypotheses included the main effects of group (BEEP versus comparison sample) and community (suburban versus urban location), as well as their interaction. RESULTS: Young adults from the suburban community had higher levels of educational attainment than did those in the urban group, with little difference between the suburban BEEP and comparison groups. In the urban group, participation in the BEEP program was associated with completing >1 additional year of schooling. Fewer BEEP young adults reported having a low income (less than 20000 dollars); the income differences were accounted for largely by the urban participants. The percentage of subjects with private health insurance was significantly lower in the urban group overall, but the BEEP urban group had higher rates of private insurance than did the comparison group. More than 80% of both suburban samples reported being in very good or excellent health; the 2 urban groups had significantly lower ratings, with 64% of the BEEP group and only 41.67% of the comparison group reaching this standard. Overall, suburban participants reported more positive health behaviors, more perceived competence, and less depression. Among the urban samples, however, participation in BEEP was associated with higher levels of health efficacy, more positive health behaviors, and less depression than their peers. CONCLUSIONS: No previous study has focused as extensively on health-related outcomes of early education programs. BEEP participants living in urban communities had advantages over their peers in educational attainment, income, health, and well-being. The educational advantages found for BEEP participants in the early years of schooling included executive skills such as planning, organizing, and completing school-related tasks. It is likely that these early advantages in executive function extended beyond education-related tasks to other activities as participants became responsible for their own lives. The long-term benefits revealed in this study are consistent with the findings of previous long-term studies that indicated that participants in high-quality intervention programs are less likely to cost taxpayers money for health, educational, and public assistance services. The BEEP program appears to have somewhat blunted differences between the urban and suburban groups. The results of this study add to the growing body of findings that indicate that long-term benefits occur as the result of well-designed, intensive, comprehensive early education. The health benefits add a unique and important extension to the findings of other studies.

Adult↗

A comparison of predictors of success on NCLEX-RN for African American, foreign-born, and white baccalaureate graduates.

This retrospective study was concerned with identifying the strongest predictors of success for African American and foreign-born baccalaureate graduates on the NCLEX-RN from the following nine variables--the admission grade point average, medical-surgical nursing grade point average, nursing grade point average, cumulative grade point average, percentile rank on the Mosby Assess Test, age at the time of the licensing examination, number of semesters needed to complete the nursing curriculum, licensed vocational nurse status, and the number of Ds and Fs received in nursing courses--and comparing these with predictors of success for white baccalaureate graduates. Three random samples of 50 African American, foreign-born, and white graduates from four baccalaureate schools from May 1987 through May 1992 were selected from a total population of 1,205. Chi-square, Fisher's exact test, two-way analysis of variance, and discriminant analysis were used to analyze the data. Students in all three ethnic groups with a Mosby Assess Test percentile rank below 21 and a D or F in a nursing course were more likely to fail the NCLEX-RN than those with a higher percentile rank and no Ds or Fs.

Black or African American↗

Towards AIDS prevention and education at the University of Malawi.

It is important to develop community orientated approaches to health promotion. Malawi has one of the highest rates of AIDS/HIV infection and one of the lowest percentages of students in tertiary education, in the world. We surveyed the opinions of 175 undergraduate university students on developing prevention and education regarding HIV/AIDS. Descriptive statistics, analysis of variance and factor analysis revealed several important findings: there were no consequential differences between the responses of male and female students; information on self protection was rated as most important for AIDS/HIV education; the primary factor for AIDS/HIV prevention also related to protection, but this was in conjunction with a desire for "sex-perpetuation". These findings should contribute to the development of health promotion initiatives, directed at AIDS/HIV, which reflect the values and concerns of the university student community. Such an approach acknowledges the role of students as a major 'stakeholder' in the university community, rather than dissempowering them as simply the recipients of 'appropriate AIDS/HIV prevention/education'.

Acquired Immunodeficiency Syndrome↗

Paracervical block diminishes cramping associated with cryosurgery.

BACKGROUND: The choice of treatment method for cervical intraepithelial neoplasia can be dictated by the lesion size, by comfort of the operator with the technique, by the cost of the procedure, and by patient comfort with the procedure. The purpose of this research was to compare the usual method of cryosurgery (no anesthetic block) with a method using a paracervical block to reduce the pain and cramping associated with cryosurgery. METHODS: A prospective trial was designed and conducted in a colposcopy clinic. Of the 85 women enrolled in the study, all were immediately given 550 mg of naproxen sodium orally; 40 received no block and 45 received a paracervical block before the cryosurgery procedure. After the procedure, a trained interviewer elicited pain and cramping scores using a visual analog scale. Chi-square, Fisher's exact test, Mann-Whitney U, Wilcoxon signed-ranks test, Friedman's two-way analysis of variance, and multivariate analysis of variance with covariates were used to analyze the data. RESULTS: Each part of the double-freeze cryosurgical procedure was ranked according to the participants' perceptions of pain and cramping. The cramping after the first freeze was significantly less for women receiving the paracervical block than for the women undergoing the usual procedure (z = -2.44, P = .014). Including the discomfort from the injection itself, the women who received a paracervical block perceived less cramping overall during cryosurgery than the women with no block (z = -2.35, P = .019). The paracervical block did not decrease the pain from cryosurgery according to the participants' rankings of perceived pain. CONCLUSIONS: A paracervical block is effective in reducing the cramping from cryosurgery.

Cervix Uteri↗

Components-of-variance models for random-effects ROC analysis: the case of unequal variance structures across modalities.

RATIONALE AND OBJECTIVES: Several of the authors have previously published an analysis of multiple sources of uncertainty in the receiver operating characteristic (ROC) assessment and comparison of diagnostic modalities. The analysis assumed that the components of variance were the same for the modalities under comparison. The purpose of the present work is to obtain a generalization that does not require that assumption. MATERIALS AND METHODS: The generalization is achieved by splitting three of the six components of variance in the previous model into modality-dependent contributions. Two distinct formulations of this approach can be obtained from alternative choices of the three components to be split; however, a one-to-one relationship exists between the magnitudes of the components estimated from these two formulations. RESULTS: The method is applied to a study of multiple readers, with and without the aid of a computer-assist modality. performing the task of discriminating between benign and malignant clusters of microcalcifications. Analysis according to the first method of splitting shows large decreases in the reader and reader-by-case components of variance when the computer assist is used by the readers. Analysis in terms of the alternative splitting shows large decreases in the corresponding modality-interaction components. CONCLUSION: A solution to the problem of multivariate ROC analysis without the assumption of equal variance structure across modalities has been provided. Alternative formulations lead to consistent results related by a one-to-one mapping. A surprising result is that estimates of confidence intervals and numbers of cases and readers required for a specified confidence interval remain the same in the more general model as in the restricted model.

Analysis of Variance↗

Interpretation of computed tomography does not correlate with laboratory or pathologic findings in surgically confirmed acute appendicitis.

BACKGROUND: Computed tomography (CT) is used increasingly to diagnose acute appendicitis, despite variable technique and interpretation. We hypothesized that CT interpretation would not reflect actual clinical-pathologic findings in all demographic patient groups. METHODS: A prospective university hospital database of 625 consecutive patients (1995-1999), all of whom were operated on for appendicitis (261, or 41.8%, within 24 hours of discretionary CT), was reviewed. CT and pathology data were obtained from final, written reports. CT criteria included free fluid or air, appendiceal visualization, mesenteric fat stranding, and blurred pericecal fat. Appendix pathology included acute, gangrenous, and perforated organs. Statistics were performed with the Fisher exact test (coordinate data) and univariate analysis of variance (continuous data); multivariate analysis of variance for independent effects on dependent variable (positive CT or pathology; P <.05). RESULTS: The mean age was 35 +/- 1 years with 46.6% being female patients. CT was done more often in women and after 1997 (both P <.05). The sensitivity and specificity of CT were 96.1% and 16.1%, respectively. The positive predictive value (PPV) and accuracy rate (A) were 90%, and 88%, respectively. After CT, the incidence of finding a normal appendix was lower (19.3% vs 12.3%, P <.05), especially if the white blood cell count (WBC) was normal (< or = 11K/microL, 6.1% vs 23.2%, P <.001). If the WBC was < or = 11K/microL with positive CT, PPV/A was 73. 7%/71.3%, whereas with WBC > 11K/microL and positive CT, PPV/A was 99.4%/93.3%. Multivariate analysis of variance showed that none of the individual variables used by the radiologist to determine a positive CT scan correlated with outcome determined by surgical pathology. A healthy appendix was predicted by a CT interpreted as negative and younger age (both P <.05), and especially by lower WBC (P <.0001), but not by gender or surgeon. CONCLUSIONS: Although the negative appendectomy rate was decreased by CT, there was no correlation between CT findings and pathologically proved disease. Other factors such as more precise patient selection by clinical criteria may also be improving outcome. A positive CT scan in a patient with a normal WBC should be interpreted with caution.

Acute Disease↗

Covariance analysis of laboratory variance in steady-state serum phenytoin concentrations.

Inpatients (n = 57) on long term prophylaxis with 2 oral phenytoin preparations were followed up via monthly checks of serum drug concentrations. Duplicate serum aliquots were submitted to 2 laboratories, and covariance analysis was used to estimate laboratory error. The laboratory-associated variance of examinations using the 'EMIT' or 'TDX' systems was 7.1 to 10 (mg/L)2, while that of chromatographic assays was 37.1 (mg/L)2. Laboratory errors were distributed in a non-normal fashion. The ratio of the maximum rate of metabolism (Vmax) and the Michaelis-Menten constant (Km) tended to remain constant between individuals. A linear analytical model showed little more residual variance than one based on Michaelis-Menten pharmacokinetics.

Administration, Oral↗

Cephalometric predictors of long-term stability in the early treatment of Class III malocclusion.

The aim of this study was to examine the differences in the early craniofacial morphology of Class Ill malocclusions. Lateral cephalograms of 45 subjects with a Class III malocclusion and an anterior crossbite in the deciduous or mixed dentition were examined before treatment, after treatment, and during the long-term retention stage. The anterior crossbites of all patients were corrected after a series of orthodontic treatments. After a mean follow-up period of 5.7 years, all the subjects were reevaluated and divided into three groups according to the final occlusal status: good, fair, and poor occlusal stability. Twenty cephalometric variables on the pretreatment lateral cephalograms were analyzed by one-way analysis of variance and discriminant analysis to identify the key determinants for discriminating among the three groups. Among the 20 variables, 11 showed statistical significance. Generally, the subjects with a smaller gonial angle and a more hypodivergent skeletal pattern had good prognosis after the early treatment of Class III malocclusion. When the AB to mandibular plane angle and N-perpendicular to point A were selected in discriminant analysis, the AB to mandibular plane angle was the most significant variable. Discriminant analysis showed a relatively high degree of correct classifications of the patients with early Class III malocclusion. In particular, discriminant analysis showed the highest accuracy (93.3%) when predicting a poor prognosis.

Adolescent↗