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Studies on isolation of a toxic substance from human and animal skin burned in vitro.

Attempts to obtain burn toxin from animal and human skin are reported. Mouse skin was burned with a metal stamp by the method of Allgöwer. The human and the skin of various experimental animals was scalded in NaCl solution by the method of Rosenthal. Using the latter method it was possible to obtain the so-called "crude burn toxin" from the human skin. It was highly lethal to mice, rats, rabbits and guinea pigs after toxic. No toxic preparations were obtained from the skin of various experimental animals using the method of burning and processing described by Rosenthal.

Adolescent↗

Meal timing alters circadian rhythm of skin surface temperature in non-human primates.

Circadian rhythms of skin surface temperature were demonstrated in a group of African green vervets. Alteration of the timing of a single daily meal of fixed caloric content resulted in a shift of the skin surface temperature acrophase in 7 of the 8 series. The timing of the individual temperature acrophases in relation to the light-dark cycle and the extent of acrophase shift in response to alteration of meal timing showed considerable variation between the individual animals studied.

Animals↗

[Epidemiological study of arboviruses in the Central African Republic: demonstration of Chikungunya virus during 1978 and 1979].

From January 1978 to May 1979, the Chikungunya virus came out in several places in south of Central African Republic. We isolated one strain of virus from a human serum and 33 from pools of Aedes africanus group collected in two forest galleries situated near the village of Bozo, in the semi-humid savanna. Several serological conversions were observed in patients with fever joint pain and sometimes macular eruption. The intensive activity of Chikungunya virus was confirmed by serological survey in south-east of the country, i. e.: 17% sera were positive by IHA test with high level (greater than or equal to 1 280) according to recent infection. Mosquitoes strains isolated in 1978 seem to proceed from epizootic manifestation, as we observed in 1975 in the same area. Epidemiologic findings of periodic recurrence is discussed.

Aedes↗

[Further studies on the sinus coronarius cordis, vena cordis media and arcus aortae as well as on the ductus (lig.) Botalli in various mammalian hearts].

This study supplements earlier works on the topography of the mammalian heart. The material comprises the hearts of 25 species, 12 of which have not been examined previously. The general morphology of the hearts is briefly dealt with. V. cava cranialis sinistra is preserved in Phascolomys and Macropus. It is presumably present in all Ruminantia, where it has been reduced to the sinus coronarius cordis in Perissodactyla. A particular emphasis is laid on the fact that the paired A. carotides and Aa. subclaviae originate from a single common stem, truncus brachio-cephalicus, in Rangifer, Ovibos, Cervus axis, Bos banteng, Okapi, the Perissodactyla, and in Dicerorhinus sumatrensis and Viscacia. A common origin of the carotids and the A. subclavia dextra is frequently found in most other groups. The fusion seems occur through wandering of the vessels towards the right - i.e. towards A. subclavia dextra. Separate origins of the 4 beffore-mentioned stems occur rarely and only incompletely. Sinus coronarius is of a typical shape. Most probable V. anonyma is always present.

Animals↗

Leucocyte migration inhibition test as an evidence of immunological response to measles virus. III. Statistical analysis.

Two-way analysis of variance with replications was used for comparing the size of leucocyte migration areas in the samples of blood of monkeys before and after immunization of animals with measles virus. The areas in control samples were compared with those with antigen. Size of migration areas differed significantly between control samples and those with antigen only after the immunization of animals. Interaction between individual monkeys and presence or absence of antigen was significant only 28 days after the immunization of animals.

Analysis of Variance↗

A survey of Sarcocystis in nonhuman primates.

Of the 375 wild-caught Old World and New World monkeys examined in this survey, 79 (21%) were infected with Sarcocystis, while 369 laboratory-born animals were negative. Four strucutrally different types of sarcocysts were observed; 2 were probably new species. New host records were recognized for 4 species of Asian macaques, 3 Africican cercopithecids, and the New World Saimiri sciureus.

Animals↗

Morphological changes in the articular cartilage after meniscectomy. An experimental study in the monkey.

The medial meniscus was resected from the right knees of twelve young grivet monkeys that were killed at intervals of twenty-one to 252 days after operation. The knees operated upon and the control knees were investigated radiologically and histologically. Degenerative changes occurred in the medial femoral and tibial condyles. At first there was loss of cells from the superficial layer of the articular cartilage, with a marked decrease in the acid mucopolysaccharide content of the matrix. The chondrocytes in the deeper layer of the non-calcified zone proliferated to form clones before finally degenerating. The acellular cartilage showed splitting, and with progress of the degenerative process there was thinning and erosion of the cartilage. Eventually there was complete loss of articular cartilage with thickening and exposure of the subchondral bone. These degenerative changes were confined to a small area of the articular cartilage and had occurred despite regeneration of the meniscus. The rest of the cartilage looked normal. It is concluded that articular cartilage deprived of the protection of a meniscus may undergo arthritic changes.

Animals↗

Rhesus and African green monkey leukocyte response to steroids, antithymocyte globulin, and 60Co treatments.

Rhesus and African green monkeys were treated with dexamethasome, antithymocyte globulin, 60Co irradiation, or combinations of those immunosuppressive agents, and the leukocyte response was followed. The lymphocyte population could be significantly depressed with 60Co, but dexamethasone was required to maintain a low lymphocyte count. The ability of the immunosuppressed animals to support the growth of transplanted tumor cells was markedly enhanced by the addition of ATG to the treatment schedule, even though ATG had no effect on the numbers of leukocytes in the peripheral circulation.

Adenocarcinoma↗

[Pseudotuberculosis in simians].

Pathologo-anatomic picture of the disease caused by Pasteurella pseudotuberculosis in 20 red monkeys and 2 green marmosets are described. An intestinal form of the infection was observed in the majority of the animals. The main manifestations of pseudotuberculosis in the monkeys were: lesions of the gastro-intestinal tract in the form of grave, occasionally ulcerative enterocolitis; enlargement of the spleen with the presence therein and in the liver of necroses and low granulomatous reaction; enlargement of the lymph nodes, mesenteric in particular, with the characteristic macrophagal reaction in them; impairment of the blood circulation in organs with haemorrhages and necroses, with colonies of microbes in tissues. In the course of bacteriological investigations the pathogen Pasteurella pseudotuberculosis was isolated from the perished animals.

Animals↗