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Microsystems incorporate a combination of microfluidic, micromechanical, micro-optical and microelectronic components. This article explores the impact the technology is likely to have on diagnostic and monitoring devices during the next decade.
We demonstrate a method for generating flow within a microfluidic channel using an optically driven pump. The pump consists of two counter rotating birefringent vaterite particles trapped within a microfluidic channel and driven using optical tweezers. The transfer of spin angular momentum from a circularly polarised laser beam rotates the particles at up to 10 Hz. We show that the pump is able to displace fluid in microchannels, with flow rates of up to 200 microm(3) s(-1) (200 fL s(-1)). The direction of fluid pumping can be reversed by altering the sense of the rotation of the vaterite beads. We also incorporate a novel optical sensing method, based upon an additional probe particle, trapped within separate optical tweezers, enabling us to map the magnitude and direction of fluid flow within the channel. The techniques described in the paper have potential to be extended to drive an integrated lab-on-chip device, where pumping, flow measurement and optical sensing could all be achieved by structuring a single laser beam.
OBJECTIVES: To develop a new type of optical computer-aided surgery (CAS) device that overcomes some of the restrictions of common systems and to examine its accuracy and usability under laboratory and intraoperative conditions. STUDY DESIGN: Prospective study using laboratory experiments and intraoperative data collection. METHODS: An optical CAS system applying passive optical markers for coordinate determination was developed. Laboratory accuracy measurements were obtained on a Plexiglas model with known coordinates of fiducial markers, before and after predefined table movements. Intraoperative accuracy measurements were recorded from 24 patients undergoing endonasal surgery of the paranasal sinuses with two different referencing techniques (fiducial markers and mouthpiece). RESULTS: The system demonstrated laboratory accuracy to within 0.86 mm (SD = 0.94 mm). After table movements, the accuracy decreased to 1.12 mm (SD = 0.99 mm), 1.05 mm (SD = 0.96 mm), 1.15 mm (SD = 1.04 mm), and 1.54 mm (SD = 1.25 mm), respectively, in four different positions. Intraoperative accuracy was within 1.14 mm (SD = 0.57 mm) (fiducial markers) and 2.66 mm (SD = 1.89 mm) (mouthpiece) (P < .05). One of the main advantages of the new technology was the possibility of using any common instrument or endoscope by adapting a marker array. CONCLUSIONS: Passive-marker technology has been demonstrated to be useful for optical position determination in computer-aided surgery.
PURPOSE: To examine whether objective performance of near tasks is improved with various electronic vision enhancement systems (EVES) compared with the subject's own optical magnifier. DESIGN: Experimental study, randomized, within-patient design. METHODS: This was a prospective study, conducted in a hospital ophthalmology low-vision clinic. The patient population comprised 70 sequential visually impaired subjects. The magnifying devices examined were: patient's optimum optical magnifier; magnification and field-of-view matched mouse EVES with monitor or head-mounted display (HMD) viewing; and stand EVES with monitor viewing. The tasks performed were: reading speed and acuity; time taken to track from one column of print to the next; follow a route map, and locate a specific feature; and identification of specific information from a medicine label. RESULTS: Mouse EVES with HMD viewing caused lower reading speeds than stand EVES with monitor viewing (F = 38.7, P <.001). Reading with the optical magnifier was slower than with the mouse or stand EVES with monitor viewing at smaller print sizes (P <.05). The column location task was faster with the optical magnifier than with any of the EVES (F = 10.3, P <.001). The map tracking and medicine label identification task was slower with the mouse EVES with HMD viewing than with the other magnifiers (P <.01). Previous EVES experience had no effect on task performance (P >.05), but subjects with previous optical magnifier experience were significantly slower at performing the medicine label identification task with all of the EVES (P <.05). CONCLUSIONS: Although EVES provide objective benefits to the visually impaired in reading speed and acuity, together with some specific near tasks, some can be performed just as fast using optical magnification.
An interesting case of systemic lupus erythematosus (SLE) is presented in which the clinical onset of myelopathy strongly suggested demyelinating disease. Later, intracranial hypertension developed on two occasions. Postmortem examination showed the systemic lesions of SLE. In addition, demyelinating lesions were found in the spinal cord and the optic nerve, the neuropathologic picutre of Devic's syndrome, a variant of multiple sclerosis. We question whether some of the cases showing necrotic myelopathy might have an autoimmune pathogenesis.
We have developed a method for the quantitative 3-dimensional profiling of micron sized channel networks within optically transparent "lab-on-a-chip" microreactor devices. The method involves capturing digitised microscope images of the channel network filled with an optically absorbing dye. The microscope is operated in transmission mode using light filtered through a narrow bandpass filter with a maximum transmission wavelength matching the wavelength of the absorbance maximum of the dye solution. Digitised images of a chip filled with solvent and dye solution are analysed pixel by pixel to yield a spatially resolved array of absorbance values. This array is then converted to optical path length values using the Beer-Lambert law, thereby providing the 3D profile of the channel network. The method is capable of measuring channel depths from 10 to 500 microm (and probably even smaller depths) with an accuracy of a few percent. Lateral spatial resolution of less than 1 microm is achievable. It has been established that distortion of the measured profiles resulting from a mismatch in refractive index between the dye solution and the glass of the microreactors is insignificant. The method has been successfully used here to investigate the effects of thermal bonding and etch time on channel profiles. The technique provides a convenient, accurate and non-destructive method required to determine channel profiles; information which is essential to enable optimisation of the operating characteristics of microreactor devices for particular applications.
An instrument using an electro-optical Fourier method for measuring the modulation transfer function (MTF) of low power telescopes and telemicroscopes is described. Because these devices are afocal, or nearly so, relay optics are needed to form real images at the detection section of the apparatus. The system is capable of measuring the MTF in monochromatic and white light, at any target azimuth, across the field of view, and through focus. The target system contains 14 square-wave gratings with spatial frequencies that range from 2.5 to 156 cpd. Images of these gratings are scanned across a slit. The output data are fed to a first-order recursive digital Butterworth bandpass filter for MTF analysis. The apparatus is diffraction limited at f/31.4. Therefore, it negligibly affects the measurement of the MTF of telescopes and telemicroscopes tested with exit pupils of up to 6.4 mm.
We demonstrate, for the first time to our knowledge, optical bistability on a highly integrated silicon device, using a 5-microm-radius ring resonator. The strong light-confinement nature of the resonator induces nonlinear optical response with low pump power. We show that the optical bistability allows all-optical functionalities, such as switching and memory with microsecond time response and a modulation depth of 10 dB, driven by pump power as low as 45 microW. Silicon optical bistability relies on a fast thermal nonlinear optical effect presenting a 500-kHz modulation bandwidth.
OBJECTIVES: To assess the feasibility of reshaping cartilage using radiofrequency (RF) heating, and to examine the effects of this process on tissue biophysical properties (optical and thermal) and cellular viability. METHODS: Mechanically deformed porcine septal cartilage was reshaped using 2 RF-generating devices. We performed dynamic measurements of tissue thermal and optical properties while heating cartilage with one of these devices. Cellular viability was assessed immediately and 7 days after treatment. RESULTS: A characteristic change in the diffuse transmittance of light through the cartilage occurred during heating. Change in transmittance has been shown to accompany the onset of stress relaxation in cartilage. Peak radiometric surface temperature during heating was 88.6 degrees C. Specimens retained their user-specified curved shape for the observed period of 14 days. Chondrocyte viability in RF-heated tissue was 19% and 14% of that in untreated control specimens at days 0 and 7 after treatment, respectively. CONCLUSIONS: Radiofrequency heating has been shown to effectively reshape cartilage while maintaining cellular viability, illustrating a novel application for a widely used technology.
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BACKGROUND: In the treatment of critically ill patients, blood volume (BV) measurement requires injection of some tracer substance and subsequent blood sampling to analyze the tracer concentration. To obviate both the sampling and laboratory analysis, techniques of pulse oximetry have been adapted to the noninvasive optical measurement in the patient's nose or finger of the arterial concentration of an injectable dye. METHODS: The authors report the clinical accuracy of a new noninvasive bedside BV measurement test that uses pulse spectrophotometry (the pulse method). The device detects pulsatile changes of tissue optical density of a nostril or a finger spanned by a probe emitting two infrared wavelengths (805 and 890 nm). After a peripheral or central intravenous injection of indocyanine green, the arterial dye concentration is continuously computed by reference to the previously measured blood hemoglobin concentration. Three types of tests of its accuracy are described here. RESULTS: In 10 healthy volunteers, the authors compared BV determined by the pulse method with an (131)I-labeled human serum albumin method. Three subject data sets were excluded because of motion artifact, a low signal:noise ratio, or both. For the other seven volunteers, the bias+/-SD of pulse spectrophotometric BV values were 0.20+/-0.24 l (or 4.2+/-4.9%) for the nose probe and 0.34+/-0.31 l (or 7.3+/-6.9%) for the finger probe, with a mean BV of 5 l. In 30 patients who underwent cardiac surgery, the pulse method was compared with a standard indocyanine green method using intermittent blood sampling. In three patients, the BV could not be determined by the pulse method because of motion artifact, low signal:noise ratio, or both. In 27 patients, the bias+/-SD of the BV by the pulse method was -0.23+/-0.37 l (-5.3+/-8.7%) for the nose and -0.25+/-0.5 l (-4.2+/-8.4%) for the finger. Patient BV ranged from 2.51 to 7.13 l (mean, 4.48 l). In 10 additional patients before cardiac surgery, BV was measured by the pulse method before and shortly after removal of 400 ml blood. The pulse method recorded a decrease of BV of 480+/-114 mL Three days after venesection, the mean BV was 117+/-159 ml less than the predonation control. CONCLUSIONS: In most patients, the pulse method provides bedside measurement of BV without blood sampling (except for hemoglobin determination), with an estimated error less than 10%. In 10-30% of tests the method failed because of motion distortion of the record during the 10-min data collection period or because of insufficient pulse amplitude in the test tissue.
We report on the characterization of nanometer-scale resonators. Each device incorporates one multiwalled carbon nanotube (MWNT) as a torsional spring. The devices are actuated electrostatically, and their deflections, both low frequency and on resonance, are detected optically. These are some of the smallest electromechanical devices ever created and are a demonstration of practical integrated MWNT-based oscillators. The results also show surprising intershell mechanical coupling behavior in the MWNTs.
An essential component in the execution of image-guided surgery is a hand-held probe whose spatial position is tracked during the procedure and displayed on a three-dimensional operative workstation. This paper describes an experiment performed in order to compare the accuracy of a mechanically linked pointing device (FARO surgical arm) and an optical position tracker (OPTOTRAK) against a "gold standard."
Inflammatory optic neuropathies are common in clinical practice. Monosymptomatic optic neuritis has important implications for the development of multiple sclerosis. In the patient presenting with monosymptomatic optic neuritis, MR imaging provides critical prognostic information concerning the development of MS. The ONTT provided valuable information regarding the natural history and therapy of optic neuritis. Oral prednisone alone is contraindicated in the treatment of optic neuritis because of its association with increased recurrence rate of optic neuritis. Intravenous methylprednisolone remains a viable treatment option to slightly increase the rate of recovery and provide a degree of short-term protection against the subsequent development of MS. Other inflammatory optic neuropathies include sarcoidosis, neuroretinitis, and Devic's disease with each possessing distinct clinical characteristics and treatment approaches.
Accurate targeting of diseased and healthy tissue has significantly been improved by MRI/CT-based navigation systems. Recently, intraoperative MRI navigation systems have proven to be powerful tools for the guidance of the neurosurgical operations. However, the widespread use of such systems is held back by the costs, the time consumption during operation, and the need for MR-compatible surgical devices. Raman spectroscopy is a nondestructive optical technique that enables real-time tissue identification and classification and has proved to be a powerful diagnostic tool in a large number of studies. In the present report, we have investigated the possibility of distinguishing different brain structures by using a single fiber-optic probe to collect Raman scattered light in the high-wavenumber region of the spectrum. For the Raman measurements, 7 pig brains were sliced in the coronal plain and Raman spectra were obtained of 11-19 anatomical structures. Adjacent brain structures could be distinguished based on their Raman spectra, reflecting the differences in their biochemical composition and illustrating the potential Raman spectroscopy holds as a guidance tool during neurosurgical procedures.
UNLABELLED: Studies comparing efficacy of fibre optic phototherapy to conventional phototherapy are performed mostly in term infants and give conflicting results. This randomized prospective study compares efficacy of fibre optic phototherapy using the Ohmeda Biliblanket device to conventional fluorescent phototherapy in preterm infants. A total of 124 preterm infants with a nonhaemolytic hyperbilirubinaemia were evaluated. Stratification at randomisation was performed according to birth weight ( < 1000 g, 1000-1500 g or 1500-2000 g). Fifty-six infants received fibre optic and 68 conventional phototherapy. Efficacy was assessed by comparing the required duration of phototherapy. Median duration of phototherapy was 118 h and 114 h in the fibre optic and conventional groups respectively, the difference in which was not statistically significant. The median durations were also not significantly different within the separate weight groups. The number of infants requiring exchange transfusions was similar in both treatment groups. CONCLUSION: The efficacy of fibre optic phototherapy in preterm infants is comparable to conventional phototherapy.
A radar-based proximity warning system was evaluated by researchers at the Spokane Research Laboratory of the National Institute for Occupational Safety and Health to determine if the system would be effective in detecting objects in the blind spots of an off-highway dump truck. An average of five fatalities occur each year in surface mines as a result of an equipment operator not being aware of a smaller vehicle, person or change in terrain near the equipment. Sensor technology that can detect such obstacles and that also is designed for surface mining applications is rare. Researchers worked closely with the radar system manufacturer to test and modify the system on large, off-highway dump trucks at a surface mine over a period of 2 years. The final system was thoroughly evaluated by recording video images from a camera on the rear of the truck and by recording all alarms from the rear-mounted radar. Data show that the system reliably detected small vehicles, berms, people and other equipment. However, alarms from objects that posed no immediate danger were common, supporting the assertion that sensor-based systems for proximity warning should be used in combination with other devices, such as cameras, that would allow the operator to check the source of any alarm.