Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Migration”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,441 records · Page 80Linked to original sources
PubMed2004

Influence of endograft oversizing on device migration, endoleak, aneurysm shrinkage, and aortic neck dilation: results from the Zenith Multicenter Trial.

BACKGROUND: Generous endograft oversizing has been associated with propensity for aortic neck dilation and subsequent device migration in endografts without suprarenal fixation. Effects of variable oversizing of endografts with suprarenal fixation have been poorly studied. METHODS: Three hundred fifty-one patients underwent endovascular AAA repair (EVAR) in a prospective multicenter trial using the Zenith AAA Endovascular Graft, a fully supported bifurcated 3-piece endograft with barb-enhanced suprarenal stent fixation. Blinded core-laboratory measurement of variables was prospectively recorded at predischarge and at 1, 6, 12, and 24 months after the procedure. Potential influence of endograft oversizing on subsequent aortic neck dilation (minor axis), aneurysm shrinkage (major axis), device migration, endoleak, rupture, open conversion, and death were retrospectively studied. Data are given as mean +/- SEM. RESULTS: Risk of endograft migration (>5mm) at 12 months was 2.3% (6/261). However, patients with endograft oversizing of >30% had a 14% (4/29) migration risk compared with those oversized < or =30% (0.9%, 2/232), P <.002. There was zero device migration by the SVS definition (>10 mm or clinical event). Device oversizing >30% was associated with decreased AAA sac shrinkage (48% vs 77%) and with increased sac enlargement (9.5% vs 0.6%) at 24 months when compared with oversizing of < or =30%, respectively (P =.001). Incidence rate of any endoleak at 12 and 24 months was 8.2% (21/256) and 7.1% (12/169), respectively. Oversizing of endografts by >30% was associated with an increased type II endoleak rate (11 vs 4.7%) that failed to reach statistical significance (P =.27). Aortic neck diameters increased significantly by 6 months (P <.001) but then stabilized through 24 months; the absolute changes at 1 (n = 298), 6 (n = 278), 12 (n = 264), and 24 months (n = 171) were 0.66 +/- 0.10 mm (3.0%), 1.32 +/- 0.11 mm (5.6%), 1.38 +/- 0.12 mm (5.9%), and 1.44 +/- 0.16 mm (6.1%), respectively. Linear regression analysis demonstrated no correlation between endograft oversizing and aortic neck dilation at 12 (P =.86) or 24 months (P =.64). CONCLUSIONS: Device migration and endoleaks were very infrequent after treatment with the Zenith AAA Endovascular Graft. However, endograft oversizing of >30% was associated with an approximately 14-fold increase in device migration (>5 mm) at 12 months and with a approximately 16-fold increased risk of AAA expansion at 24 months. Although further follow-up will be essential to assess whether these early associations continue, avoidance of excessive endograft oversizing is recommended.

Aorta, Abdominal↗
PubMed2005

Absence of proximal neck dilatation and graft migration after endovascular aneurysm repair with balloon-expandable stent-based endografts.

OBJECTIVE: Proximal neck dilatation (PND) and/or endograft migration with the subsequent development of type I endoleak is a significant cause of late endograft failure after endovascular abdominal aortic aneurysm repair (EVAR). Although there are numerous reports examining PND in patients receiving endografts that use self-expanding stents (SES) for proximal fixation, there are no such reports for patients treated with endografts that use balloon-expanding stents (BES). The purpose of this study was to investigate PND and endograft migration after EVAR with BES endografts. METHODS: We retrospectively reviewed all charts and all serial computed tomographic scans available for patients who underwent EVAR with a BES endograft (surgeon-made, aortounifemoral polytetrafluoroethylene graft with a proximal Palmaz stent) between August 1997 and October 2002. Only patients with longer than a 12-month follow-up were analyzed. Neck diameter was measured at the level of the lowest renal artery and at 5 mm below it. PND was defined as neck enlargement of 2.5 mm or more. To assess endograft migration, the distance between the superior mesenteric artery and the cranial end of the BES was measured. Stent migration was defined as a change of 5 mm or more. RESULTS: A total of 77 patients received this device during the study period. The technical success rate was 99%. The 1-, 3-, and 5-year survival was 66%, 48%, and 29.5%, respectively. Complete serial computed tomographic scans were available in 41 of the 48 patients who survived 12 months or longer after the operation. The mean follow-up period for these patients was 31 months (range, 12-66 months). The maximum aneurysm diameter was either unchanged or decreased in 35 patients (85%). The immediate postoperative proximal neck diameter was 19 to 29 mm (median, 24 mm). This was unchanged at the latest follow-up. None of the patients had significant PND. The cranial end of the BES was located in the area between 14 mm proximal and 36 mm distal to the superior mesenteric artery (median, 6 mm). None of the patients developed significant endograft migration. CONCLUSIONS: Neither PND nor endograft migration was observed with the BES endograft. The nature of the SES may be responsible for the observed neck dilatation and device migration after EVAR with SES endografts. This study suggests that BES may be a better fixation method for EVAR.

Aged↗
PubMed2006

An evaluation of centerline of flow measurement techniques to assess migration after thoracic endovascular aneurysm repair.

OBJECTIVE: To validate a means by which migration of thoracic stent grafts can be reliably detected and quantified. METHODS: Patients treated for thoracic aneurysms (without dissections) with either the Cook Zenith TX1/TX2 or the Gore TAG device were retrospectively reviewed. Patients with digital imaging data at a baseline study (discharge or 1-month computed tomographic scan) and a minimum of 6 months' follow-up were evaluated on a three-dimensional workstation. Centerline of flow (CLF) calculations were used to determine length measurements to establish distances from native vascular landmarks (left common carotid artery, left common carotid artery, and celiac artery) to the proximal and distal aspects of the fixation systems of stent grafts. Patients with evidence of fixation system migration (>10 mm of movement) or increasing thoracic aortic lengths (left common carotid artery to celiac artery distance) were subjected to more detailed reviews. RESULTS: Of 194 patients evaluated (133 Zenith and 61 TAG), 46 were treated for dissections and excluded. Fifty-seven patients did not have a digital baseline study and available DICOM data for follow-up imaging at 6 months or later or had died before such follow-up imaging. The remaining 91 patients underwent assessment for device migration. Analyses were conducted on 19 patients at 6 months, on 42 at 12 months, on 12 at 24 months, on 13 at 36 months, and on 5 at 48 months. CLF analysis noted more than 10 mm of caudal movement of the proximal device in 10 patients and cranial movement of the distal device in 3 patients. When this subset was further scrutinized with regard to morphologic changes remote from the prosthesis and in the context of the overall aortic repair (such as elephant trunk grafts), only four patients had movement of the proximal or distal fixation system with respect to the initially deployed location. Two-dimensional axial image analysis identified migration in only one of the four patients with CLF-detected fixation system movement. CONCLUSIONS: The importance of early migration detection cannot be overstated given the potential to avert consequences as evidenced by analyses of counterpart abdominal aortic aneurysm devices. In vivo thoracic device analysis is more complex than that for devices used for infrarenal aneurysms. Distance calculations based on CLF measurements may overestimate the frequency of true migration, yet they serve as a reasonable initial screening tool. The resultant subset of patients then must undergo a more detailed evaluation of device position in the context of the aortic morphology to differentiate true migration from devices that maintain stable fixation system positions.

Algorithms↗
PubMed1999

Endoscopic techniques for retrieval of proximally migrated biliary stents: the Amsterdam experience.

BACKGROUND: Endoscopic retrieval of proximally migrated biliary plastic stents may be technically challenging and sometimes unsuccessful. Despite the widespread use of such stents, there are few reports on the technique for retrieval of migrated stents. METHODS: We reviewed the records of patients who were found to have proximally migrated biliary stents during the last 3 years at a tertiary referral center. Our study analyzed the various techniques used for endoscopic retrieval and evaluated the factors influencing the choice of a particular technique. RESULTS: Endoscopic retrieval of a proximally migrated stent was attempted in 41 patients and was successful in 37 (90%). The retrieval techniques included Dormia basket, balloon, balloon and basket, basket and ball tip catheter, forceps, and Soehendra stent retriever. The Dormia basket technique was successful in the majority of cases. CONCLUSION: Proximally migrated biliary stents can be successfully removed endoscopically in most cases. The choice of a retrieval technique is dependent on several factors including biliary ductal dilatation, depth of stent migration, distal stent impaction and biliary stricture distal to the migrated stent. The placement of an additional stent alongside an irretrievable stent is a satisfactory alternative to retrieval.

Catheterization↗
PubMed2006

Failure of sacral nerve stimulation due to migration of tined lead.

PURPOSE: Stimulation of the sacral nerves is a commonly used treatment for frequency, urgency, urge incontinence, retention and other types of voiding dysfunction. Minimally invasive placement of a percutaneous permanent quadripolar tined lead into the sacral foramen has been described. No lead migration has been reported. We report on our experience with lead migration and the subsequent failure of InterStim in a large cohort of patients with a focus on possible diagnostic and salvage techniques. MATERIALS AND METHODS: Between February 2002 and April 2005 tined lead electrodes were implanted in the S3 foramen in 235 patients using the InterStim system. Patients with a good response during the testing phase (greater than 50% improvement) underwent placement of an implantable pulse generator. Position was confirmed by radiographic evaluation intraoperatively. Sacral radiographs were obtained at the first postoperative visit, after IPG placement and whenever there was a change in symptomatic response. RESULTS: There were 5 patients (2.1%) in whom treatment failed after a successful trial of stimulation due to lead migration. This was seen as early as 3 weeks and as late as 8 months. Migration of the lead occurred between first and second stage implantation in 1 of the 5 cases, and occurred after the second stage in 4 of 5. Anterior migration was noted in 4 patients and posterior migration was noted in 1. CONCLUSIONS: Lead migration after placement of the tined lead can occur and thus sacral radiographs should be routinely used. This complication can be easily resolved without significant morbidity to the patient.

Algorithms↗
PubMed2003

Bothrops lanceolatus (Fer de lance) venom stimulates leukocyte migration into the peritoneal cavity of mice.

The ability of Bothrops lanceolatus venom to induce neutrophil migration into the peritoneal cavity of mice was investigated. Intraperitoneal injection of venom caused dose- and time-dependent neutrophil migration, which peaked with 750 ng of venom/cavity 4h after venom injection. The neutrophil migration was significantly reduced by pretreatment with dexamethasone (0.5 mg/kg, s.c.), an indirect inhibitor of phospholipase A(2) (PLA(2)), and AA861 (0.01 mg/kg, s.c.), a 5-lipoxygenase inhibitor, but in contrast, was not modified by pretreatment with indomethacin (2 mg/kg, s.c.), an inhibitor of the cyclooxygenase pathway, meloxicam (5 mg/kg, s.c.), an inhibitor of the cyclooxygenase-2 pathway, or the PAF inhibitor WEB2086 (40 mg/kg, s.c.). Dexamethasone and AA861 also inhibited the neutrophil migration by 60% when administered immediately after venom injection, and the coadministration of these two drugs caused a 75% reduction in migration. BLV-induced neutrophil migration was not due to contamination by endotoxin since polymyxin B-treated venom retained its activity. Heating the venom (97 degrees C, 2 min) reduced the PLA(2) activity by 64% and this was accompanied by a corresponding reduction (68%) in neutrophil migration. These results suggest that arachidonate-derived lipoxygenase metabolites (possibly leukotriene B(4)) are involved in the chemotaxis observed. Macrophages may be an important source of these metabolites since the migratory response to venom was potentiated in mice pretreated with thioglycollate, but reduced when the peritoneal cavity was washed with sterile saline.

Animals↗
PubMed2000

Hypersensitivity to metallic biomaterials: a review of leukocyte migration inhibition assays.

Metal hypersensitivity is a well-established phenomenon occurring in a variety of domestic and workplace settings. Degradation products of metallic biomaterials may mediate metal hypersensitivity. However, little is known about the short- and long-term pharmacodynamics and bioavailability of circulating metal degradation products in vivo. Mechanisms by which in vivo metal sensitivity reactions occur have not been well characterized and the degree to which metal sensitivity may be a predisposing factor for eliciting an overaggressive immune response remains clinically unpredictable. In vitro leukocyte migration inhibition assays have been used for investigating cell-mediated hypersensitivity reactions to biomaterial and biomaterial degradation products. This review provides a historical and technical summary of four in vitro techniques used for determination of leukocyte migration activity: (1) membrane migration or Boyden chamber, (2) capillary tube, (3) leukocyte migration using agarose technique, and (4) collagen gels. It is difficult to determine which, if any, of these techniques is singularly best suited for the investigation of suspected biomaterial-related symptoms in patients. However, Boyden chamber membrane migration testing is recommended for clinical investigations, principally because a high degree of standardized investigator independent materials and methodologies is necessary for compiling and comparing the results of patients tested at various times over the length of an extended study. Ultimately, in vitro migration inhibition testing has the potential to provide a reliable means for predicting some complications and thus enhancing the outcome for patients receiving metallic implants. Continuing improvements in migration inhibition testing methods, used alone or in combination with other immunologic assays, will likely improve assessment of patients susceptible to biomaterial antigen-induced delayed-type hypersensitivity responses.

Biocompatible Materials↗
PubMed1997

Migration of the uncemented Harris-Galante acetabular cup: results of the einbildroentgenanalyse (EBRA) method.

Seventy primary total hip arthroplasties using the Harris-Galante acetabular cup (Zimmer, Warsaw, IN) were prospectively examined. Over the entire period of 65.4+/-7.8 months, radiologic migration analysis was performed using the Einbildroentgenanalyse (EBRA) method at an accuracy of 1 mm. Although there was clinically no suspicion of prosthetic loosening in any case, in 8 implants (11.4%) migration of more than 1 mm was observed. Cranial migration occurred in 3 cases (1.1, 1.3 and 1.6 mm), medial migration in 3 cases (1.1, 1.1, and 2.9 mm), and lateral migration in 2 cases (1.1 and 1.1 mm). In the other 62 cases, however, no migration was traceable. Compared with measurements of other implant systems by means of the EBRA method published recently by other groups, the migration rate of the Harris-Galante cup was without exception lower and provided excellent midterm implant stability.

Acetabulum↗
PubMed2003

Inhibition of lens epithelial cell migration at the intraocular lens optic edge: role of capsule bending and contact pressure.

PURPOSE: To evaluate the inhibitory effect of a sharp intraocular lens (IOL) optic edge, a sharp capsule bend, and contact pressure between the optic edge and posterior capsule on lens epithelial cell (LEC) migration. SETTING: Department of Ophthalmology, Kyorin University, Tokyo, Japan. METHODS: This in vitro laboratory study evaluated a tumble-polished convex-plano IOL (CP group), an AcrySof IOL (Alcon) with a sharp edge (AS group), a new IOL with a round ridge (RR group), and a new IOL with a sharp ridge (SR group). The 2 new IOLs have high ridges and high angled loops that create firm contact between the ridge and posterior capsule. After sham cataract surgery, an IOL and a capsular tension ring (CTR) were implanted in the capsular bag of rabbit eyes. The extracted capsular bags containing the CTR and IOL were cultured. The inhibitory effect of each IOL on cell migration was analyzed. Furthermore, LEC migration on the posterior capsule was compared in culture between capsules having a sharp right angle and those with gradually curving bends. RESULTS: The inhibitory effect on cell migration was statistically greatest in the SR group followed by the RR, AS, and CP groups. A sharp capsule bend did not inhibit cell migration. CONCLUSIONS: The results suggest that inhibition of cell migration at the optic edge is regulated by the degree of contact pressure between the optic edge and posterior capsule. A sharp capsule bend might indicate strong contact but does not in itself inhibit cell migration.

Animals↗
PubMed1976

Demonstration of cellular immunity in chronic myeloid leukaemia using leucocyte migration inhibition assay.

Peripheral blood leucocytes from chronic myeloid leukaemia patients in remission were tested for inhibition of migration in presence of solubilized membrane antigens from leukaemic cells in 15 cases. Eight out of 9 autochthonous combinations (88-8%) and 35/49 allogenic combinations (71-4%) showed inhibition of migration. Antigens prepared from relapse leukaemic cell samples in 4 cases showed inhibition of migration of autochthonous as well as allogeneic remission leucocytes. The same batch of CML antigens inhibited migration of normal leucocytes at the level of 22-2%. The difference between inhibition of migration shown by remission leucocytes and normal leucocytes in presence of CML antigens was statistically significant. Solubilized antigens, similarly prepared from normal leucocytes, showed inhibition of migration of remission leucocytes to the extent of 15% only. The difference between the reactivity of CML remission leucocytes to normal and CML antigens was also statistically significant. No enhancement of migration of remission leucocytes was seen with CML antigens.

Antigens, Neoplasm↗
PubMed1978

Cellular immunity to encephalitogenic factor in man as measured by the macrophage migration inhibition test: the effects of serum.

Sensitivity to human encephalitogenic factor (EF) was measured in 70 cancer patients, in 34 patients with various non-malignant diseases and in 18 healthy volunteers, using the macrophage migration inhibition (MMI) test. Sensitization was demonstrated in 44/70 (63%) of the cancer patients, in 11/34 (32%) of the patients with non-malignant conditions and in one (5%) of the healthy individuals. No significant difference was seen in the frequency of demonstrable sensitivity with clinical stage of disease in cancer patients.Autologous serum from cancer patients had the ability to abrogate EF-mediated migration inhibition in 22/30 sensitized individuals. This blocking occurred with a similar frequency in all 3 clinical stages of cancer. Autologous serum from patients with non-malignant disease caused abrogation of EF-mediated migration inhibition in 4/11 sensitized individuals, whilst none of the healthy control individuals showed any significant change in the migration index in the presence of autologous serum. Homologous serum from patients with carcinoma of the breast or lung with and without autologous blocking activity and serum from a healthy individual were tested against lymphocytes from patients with various tumour types with the MMI test. Of 11 patients tested in the absence of serum, 8 (73%) showed significant migration inhibition with EF, whilst serum from patients with carcinoma of the lung or breast with autologous blocking activity abolished migration inhibition with EF in all 8 individuals with the former and in 6 with the latter, regardless of the tumour type from which the lymphocytes under test were derived. Homologous serum from both a carcinoma of the lung and breast without autologous blocking activity did not abolish migration inhibition with EF, except with the latter in one patient with a carcinoma of the lung.

Adolescent↗
PubMed1997

Radiographic assessment of cup migration and wear after hip replacement.

Methods are needed for accurate measurement of acetabular cup migration and wear after hip replacement. The EBRA (Ein Bild Röntgen Analyse) method was recently introduced as computerized method for radiographic assessment of acetabular cup migration. In this study, various standard methods for measuring migration were evaluated and compared to radiostereometry (RSA), which has proved to be highly accurate. A subroutine for wear measurement was developed and added to the EBRA method. Of the standard methods, Nunn's method was the best for migration measurement and Livermore's the best for wear measurement. Measurements with EBRA were better than the standard methods. Pelvic tilt seemed to be the main source of error in measurements. The effect of pelvic tilt was evaluated experimentally. EBRA detected and excluded tilted radiographs, the errors of measurement being smaller with EBRA than with standard methods. The precision of the input procedure, repeated radiographic examination, the intra- and interobserver errors were assessed. Apart from the digital input of the data, EBRA was better than the standard methods. Normal values concerning acetabular cup migration and wear should be obtained from long-term surviving hip replacements, without radiographic signs of loosening. No method of measurement detected evidence of changes in the wear-rate and in migration over time. EBRA showed cold-flow in some cups, but did not provide additional information in the long-term. Nunn's method for migration measurements and Livermore's method for wear are recommended in clinical practice. EBRA is more accurate and should be used for studies of new implant designs that have passed the preclinical and, preferably, radiostereometric analysis. RSA is unsurpassed and is recommended for early clinical follow-up in a limited number of patients.

Acetabulum↗
PubMed1998

Sufentanil inhibits migration of human leukocytes through human endothelial cell monolayers.

UNLABELLED: The interactions between blood and vascular wall cells are essential for understanding pathophysiological processes, e.g., during inflammation. The influence of anesthetics on leukocyte function is well documented. An inhibitory effect of thiopental, midazolam, and ketamine on leukocyte chemotaxis in a Boyden chamber chemotaxis assay (i.e., endothelial cells were not included) has been demonstrated. Little is known, however, about the influence of sufentanil on the inflammatory processes. To reach their targets in the tissue in vivo, leukocytes must interact with endothelial cell monolayers (ECMs). The aim of the current study was to investigate the influence of sufentanil on the migration of leukocytes through an ECM. Human umbilical vein endothelial cells were cultured to achieve a monolayer. Isolated polymorphonuclear leukocytes and ECM were preincubated with different concentrations of sufentanil. The rate of leukocyte migration against the chemotactic protein formyl-methyl-leucyl-phenylalanine was measured (n = 7). Sufentanil significantly reduced the amount of leukocyte migration through ECM to 77%+/-7.8% (P < 0.05 compared with control). Endothelial cells as well as leukocytes contributed to this effect: treatment of both cell types showed an additive effect. Although lower concentrations showed no effect, high concentrations reduced leukocyte migration through ECM to 61%+/-7.1%. IMPLICATIONS: Leukocytes play an important role during inflammation, and anesthetics influence leukocyte functions, e.g., respiratory burst or chemotaxis. The effect of sufentanil on transendothelial leukocyte migration has not been investigated. Therefore, we used a migration assay including endothelial cell monolayers. Sufentanil showed a reducing effect on transendothelial leukocyte migration.

Adult↗
PubMed1979

Significance of migration stimulatory factor in human renal allotransplantation.

Seventy-four recipients of related donor renal allografts were tested for the presence of cellular immunity to specific donor lymphocyte antigens using the direct migration inhibition factor (MIF) assay. Responses on the assay fell into one of the following three statistically distinct groups: (1) greater than 20% inhibition of macrophage migration, (2) nonresponsiveness, +/- 10% of control migration, and (3) greater than 12% stimulation of macrophage migration. Migration stimulation was shown to be reproducible and to correlate well with a very benign post-transplant clinical course. The production of migration stimulatory factor appears to be an immunological response analagous to the production of migration inhibition factor.

Female↗
PubMed2006

Simple rules guide dragonfly migration.

Every year billions of butterflies, dragonflies, moths and other insects migrate across continents, and considerable progress has been made in understanding population-level migratory phenomena. However, little is known about destinations and strategies of individual insects. We attached miniaturized radio transmitters (ca 300 mg) to the thoraxes of 14 individual dragonflies (common green darners, Anax junius) and followed them during their autumn migration for up to 12 days, using receiver-equipped Cessna airplanes and ground teams. Green darners exhibited distinct stopover and migration days. On average, they migrated every 2.9+/-0.3 days, and their average net advance was 58+/-11 km in 6.1+/-0.9 days (11.9+/-2.8 km d-1) in a generally southward direction (186+/-52 degrees). They migrated exclusively during the daytime, when wind speeds were less than 25 km h-1, regardless of wind direction, but only after two nights of successively lower temperatures (decrease of 2.1+/-0.6 degrees C in minimum temperature). The migratory patterns and apparent decision rules of green darners are strikingly similar to those proposed for songbirds, and may represent a general migration strategy for long-distance migration of organisms with high self-propelled flight speeds.

Animal Migration↗
PubMed1977

Leukocyte migration agarose test for the assessment of human neutrophil chemotaxis. II. Variables in the attraction assay.

Optimal conditions for the attraction assay of human neutrophil leukocytes were studied, using the leukocyte migration agarose test (LMAT). Larger migration areas were obtained toward the attractant wells when they were filled with zymosan-activiated serum (ZAS) in advance than when both the attractant and the cells were added simultaneously. Chemotactic indices (CI; the ratio between the migration rate toward attractant and that toward the control substance) decreased when the number of applied neutrophil leukocytes increased, whereas migration differentials (MD; the difference between the migration rate toward attractant and that toward the control substance) were not markedly affected. Both the spontaneous and the directed migration rates, and CI and MD of neutrophil leukocytes obtained from the same donor on different days varied markedly. When tested repeatedly, however, the neutrophil leukocytes of different donors migrated on the average rather equally toward inactivated serum, as they also did toward ZAS, and the variation of the means of CI and MD was insignificant.

Adult↗
PubMed1980

Leucocyte migration inhibition test and tuberculin hypersensitivity. "Escape" and some other factors influencing the clinical use.

Tuberculin hypersensitivity was evaluated in 71 Mantoux-positive and 58 Mantoux-negative persons by the leucocyte migration inhibition test. The capillary tube technique discriminated these two groups, but variability of results and overlapping of the groups were considerable. The shorter the incubation period, the better discrimination between the groups. Only after short incubation (2 h) was the correlation between the intensities of Mantoux reactions and migration inhibitions evident. The observed variability in migration areas, similar in the Mantoux-positives and controls, was the result of technical causes and variations in cells' ability to migrate. Further, in Mantoux-positive persons the migration progression (per min) increased during the first 2 h, but slowed down later on, more in controls than in chambers with antigen. Smaller migration areas in controls (early intervals) tended to produce lower migration indices than the larger ones. Finally, it was possible, using the standard score zeta, to calculate whether each individual result differed significantly from controls. By this procedure the intensity of cell-mediated immunity in individual patients could be estimated.

Adult↗
PubMed1990

Evaluation of the effect of azapropazone on neutrophil migration in anaesthetized swine using a multichamber blister suction technique.

1. The purpose of this study was to determine the in vivo inhibitory efficacy of azapropazone on neutrophil migration. The effects of azapropazone given at a dose of 100 mg kg-1 i.v. every 2 h on the neutrophil migration into skin inflammation sites (blister fluid) as well as into an autologous serum (+/- chemoattractant) placed above the blister surface (2nd chamber) were determined. 2. Azapropazone treatment schedule maintained blood levels at 70-100 micrograms ml-1 throughout the time course (360 min) of the experiment. 3. Azapropazone significantly inhibited (48 +/- 6%) neutrophil migration into the blister fluid (as evident from the decrease in myeloperoxidase activity). 4. Azapropazone significantly inhibited the neutrophil migration into the autologous serum either with (65 +/- 5%) or (35 +/- 6%) without the chemoattractant, formyl-methionyl-leucyl-phenylalanine (FMLP). The chemoattractant, FMLP, markedly increased neutrophil migration into the autologous serum by approximately 1.5 to 2 times the non-FMLP treated group. Azapropazone was more efficacious in inhibiting the neutrophil migration in the presence of FMLP than in its absence. 5. We conclude that azapropazone is an effective inhibitor of neutrophil migration into topically inflamed sites in anaesthetized swine.

Anesthesia↗