DETERMINATION OF THE NITROGEN FRACTIONS IN ATLAS AND MILO SORGHUM PLANTS.
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Acquiring information about the expression of a gene in different cell populations and tissues can provide key insight into the function of the gene. A high-throughput in situ hybridization (ISH) method was recently developed for rapid and reproducible acquisition of gene expression patterns in serial tissue sections at cellular resolution. Characterizing and analysing expression patterns on thousands of sections requires efficient methods for locating cells and estimating the level of expression in each cell. Such cellular quantification is an essential step in both annotating and quantitatively comparing high-throughput ISH results. Here we describe a novel automated and efficient methodology for performing this quantification on postnatal mouse brain.
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Orthotopic bladder substitution after cystectomy in women is feasible. The rate of continence is comparable to that in men who have been similarly treated. Over continence (which is common) is caused by posterior and caudal displacement of the neobladder. Prevention of this descent can reduce the incidence of this complication. Further follow-up is needed to evaluate the oncological outcome.
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