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PubMed1994

Influence of temperance on short-term mortality among alcohol-dependent men in Sri Lanka.

The influence of temperance on short-term mortality was assessed in a group of alcohol-dependent men admitted to a rehabilitation unit in Sri Lanka. Of the 234 men initially admitted to the programme, 188 were followed up for at least 3 years (mean +/- SD 49.5 +/- 12.6 months) or until death. All 188 were totally abstinent from alcohol on leaving the 6 week residential module of the programme. During follow-up, 115 relapsed to continued heavy drinking, whereas 73 remained either totally abstinent or drank infrequently. There were no significant differences in the age, duration of dependence, number of previous interventions, duration of follow-up or smoking habits between the two groups of patients. The overall mortality for the whole sample population was 9.6%. The mortality among those who relapsed to heavy drinking (13.9%) was more than five times that of those who were abstinent or drank infrequently (2.7%) (P < 0.04). Fifteen of the 16 deaths (93.8%) among those who relapsed to heavy drinking were alcohol-related, and included five suicides and accidents whilst under the influence of alcohol. Temperance significantly reduces short-term mortality in alcohol-dependent men when compared to those who relapse to continued heavy drinking. This difference was primarily due to reduction in the risk of alcohol-related deaths.

Adult↗
PubMed1997

The treatment of Philadelphia inebriates. From temperance reform to "secret cure".

Preventive education in the United States about the dangers of alcohol had failed to create a temperate society by 1840. But in that year, interest in medical treatment of the "habitual drunkard" rekindled in Philadelphia. It was the year an organization of reformed drunkards revitalized American temperance societies by emphasizing practical concern for the inebriate. The author describes Philadelphia's treatment of alcoholics from 1840 through the beginning of the 20th century, when entrepreneurs were marketing secret cures.

Alcoholism↗
PubMed1975

"Tropical pyomyositis." A diagnostic problem in temperate climates.

Pyomyositis is a common disease in tropical countries, but is believed to be rare in temperate climates. Two children who had never left the northeastern part of the United States developed single intramuscular abscesses. Pyomyositis may be more common in temperate climates than has previously been appreciated.

Abscess↗
PubMed1992

Basal-cell carcinoma in temperate and tropical Australia.

The clinical features of patients with basal-cell carcinoma (BCC) living in temperate and tropical Australia were studied. In the temperate zone the patients were considerably older on average, men outnumbered women and there were differences between the sexes in the site of the prevalent BCC. In the tropics the patients were significantly younger on average, the male-to-female ratio approached unity and there were no differences between the sexes with regard to the site of the BCC.

Adult↗
PubMed1998

Two cooperatively social populations of the theridiid spider Anelosimus studiosus in a temperate region

Social spiders are typically found only in the tropics. In laboratory tests and field observations, adult female A. studiosus in two populations in Tennessee, U.S.A., a temperate region, showed inter-attraction (grouping behaviour caused by the presence of conspecifics), greater tolerance towards conspecifics, and cooperation during prey capture and brood care. These are the three classical conditions considered typical of social spiders. The populations also had female-biased sex ratios and limited dispersal. Anelosimus studiosus can thus be regarded as a quasi-social or cooperative spider species. These populations of A. studiosus are the first social spiders to be described from typically temperate regions. Copyright 1998 The Association for the Study of Animal Behaviour Copyright 1998 The Association for the Study of Animal Behaviour.

Journal Article↗
PubMed1997

Characterization of the lysogeny DNA module from the temperate Streptococcus thermophilus bacteriophage phi Sfi21.

Phage phi Sfi21, the only temperate Streptococcus thermophilus phage from our phage collection, showed extensive DNA homology with virulent phages from lytic group I. Southern blot hybridizations demonstrated that the phi Sfi21-specific DNA was clustered in an approximately 6.6-kb-long region, the putative lysogeny module. Sequence analysis and database research identified an integrase within this module; orf 203 with homology to an anonymous orf 258 from the temperate lactococcal phage BK5-T; orf 127 and orf 122 with weak homology to the N- and C-terminal parts, respectively, of the cl-like repressor from lactococcal phages Tuc2009 and BK5-T; orf 75 with homology to a repressor protein from lambdoid phage 434 and an anti-repressor ant with homology to phage P1. The molecular arrangement of the predicted orfs in phage phi Sfi21 was very similar to that of the lactococcal phage BK5-T. The transition from phi Sfi21-specific DNA into DNA shared with virulent phages was abrupt and flanked at one side by notable DNA repeats. Sequence analysis identified a holin protein to the left of the lysogeny module. A site-specific deletion of 2.4 kb, which reproducibly transformed phi Sfi21 into a lytic phage, was localized in the lysogeny module. It was flanked at both sides by conspicuous DNA repeats. One repeat region reflected the DNA around the attP site, while the other reflected the putative genetic switch region between repressor and anti-repressor genes. S. thermophilus host Sfi1 transformed with a plasmid containing int and orf 203 showed resistance to superinfection by heterologous phages, but not by the homologous phi Sfi21. Part of the int gene could be deleted without loss of this activity, while a deletion in orf 203 resulted in loss of the phage resistance. We speculate on the possibility of a bipartite immunity system for the control of lysogeny in phi Sfi21.

Amino Acid Sequence↗
PubMed1999

The genetic relationship between virulent and temperate Streptococcus thermophilus bacteriophages: whole genome comparison of cos-site phages Sfi19 and Sfi21.

The virulent cos-site Streptococcus thermophilus bacteriophage Sfi19 has a 37,392-bp-long genome consisting of 44 open reading frames all encoded on the same DNA strand. The genome of the temperate cos-site S. thermophilus phage Sfi21 is 3.3 kb longer (40,740 bp, 53 orfs). Both genomes are very similarly organized and differed mainly by gene deletion and DNA rearrangement events in the lysogeny module; gene replacement, duplication, and deletion events in the DNA replication module, and numerous point mutations. The level of point mutations varied from <1% (lysis and DNA replication modules) to >15% (DNA packaging and head morphogenesis modules). A dotplot analysis showed nearly a straight line over the left 25 kb of their genomes. Over the right genome half, a more variable dotplot pattern was observed. The entire lysogeny module from Sfi21 comprising 12 genes was replaced by 7 orfs in Sfi19, six showed similarity with genes from temperate pac-site S. thermophilus phages. None of the genes implicated in the establishment of the lysogenic state (integrase, superinfection immunity, repressor) or remnants of it were conserved in Sfi19, while a Cro-like repressor was detected. Downstream of the highly conserved DNA replication module 11 and 13 orfs were found in Sfi19 and phiSfi21, respectively: Two orfs from Sfi21 were replaced by a different gene and a duplication of the phage origin of replication in Sfi19; a further orf was only found in Sfi21. All other orfs from this region, which included a second putative phage repressor, were closely related between both phages. Two noncoding regions of Sfi19 showed sequence similarity to pST1, a small cryptic plasmid of S. thermophilus.

Amino Acid Sequence↗
PubMed1990

Evaluation of a temperate-environment test of heat tolerance in prior heatstroke patients and controls.

It has been reported that scores from a temperate-environment step test describe the heat-tolerance status of prior heatstroke patients (HP). This investigation evaluated the ability of this temperate-environment heat-tolerance test (HTT) to indicate altered heart rate (HR) and rectal temperature (Tre) responses of HP, after 7 days of heat acclimation. On day 1, ten male HP (61 +/- 7 days post-heatstroke) and five control subjects (C) bench-stepped (0.30 m high, 27 steps.min-1) for 15 min (25.8 degrees C dry bulb, 16.2 degrees C wet bulb). On days 2-8, subjects underwent heat acclimation (40.1 degrees C dry bulb, 23.8 degrees C wet bulb; treadmill, 90 min.day-1). Heat acclimation resulted in significant decreases in final HR (152 +/- 5 vs 130 +/- 3 beats.min-1, P less than 0.025) and final Tre (38.62 +/- 0.11 vs 38.13 +/- 0.07 degrees C, p less than 0.01) in HP. One HP but no C was defined heat intolerant, exhibiting inability to adapt to daily exercise in the heat. On day 9, HP repeated HTT, exactly as performed on day 1; mean group HTT scores did not change (day 1 = 39 +/- 6; day 9 = 48 +/- 6, P greater than 0.05). All physical characteristics and physiological responses of HP (days 1, 2, 7, 9) were statistically similar (P greater than 0.05) to those of C. In contrast to heat-acclimation data, HTT scores (score less than or equal to 30) indicated that four HP were heat intolerant on day 1 and two HP were heat intolerant on day 9.(ABSTRACT TRUNCATED AT 250 WORDS)

Acclimatization↗
PubMedDate not supplied

Differences between surgical colorectal conditions seen in the temperate and tropical regions.

Geographic location of a population plays a significant role in the interpretation of symptoms of some diseases of the colon. Because diverticular disease of the colon is very rare in the tropics, frank rectal bleeding, which may be suggestive of bleeding diverticulosis when it occurs in a temperate region, is suggestive of a bleeding ileal typhoid ulcer eroding terminal branches of the superior mesenteric artery. Similarly, symptoms suggestive of ulcerative colitis in a temperate region are suggestive of amebic colitis in the tropics. Anatomic variation also plays a part in the nature of some disease processes. The high incidence of freely mobile cecum and ascending colon and the freely mobile redundant sigmoid colon, seen in a tropical population, play a part in the frequent occurrence of nontumid intussusception, relatively high incidence of sigmoid volvulus, and relatively high incidence of rectal prolapse seen in the area.

Climate↗
PubMed2003

Molecular marker dissection of rice (Oryza sativa L.) plant architecture under temperate and tropical climates.

Rice ( Oryza sativa L.) plants develop vertically with shoot elongation and horizontally with tillering. The purpose of this study was to identify and characterize genomic regions influencing the rice plant architecture by quantitative trait locus (QTL) analysis for the component traits: culm length (CL), panicle length (PnL), panicle number (PnN) and tiller number (TN). For this QTL analysis, 191 recombinant inbred lines (F(7)) derived from a cross of Milyang 23 (M23) and Akihikari (AK) were grown in 1995, 1996 and 1997 (May-Oct) in Joetsu, Japan (temperate climate), and in the 2000 dry season (Jan-Apr), the 2000 wet season (Jun-Oct) and the 2001 dry season in Los Baños, The Philippines (tropical climate). Results showed that rice plant architecture was influenced by 19 genomic regions categorized into five groups. In Group I, two regions (on chrs. 6 and 11) affected shoot elongation (CL and PnL) and tillering (PnN and TN) in opposite directions more significantly in Los Baños than in Joetsu. In Group II, two regions (chrs. 3 and 12) affected shoot elongation, whereas in Group III, five regions [chrs. 1 (two), 2, 3 and 9] affected only culm length (CL). Expressions of four regions of Group III were influenced by either tropical or temperate environments. In Group IV, seven regions (chrs. 1, 2, 4, 5, 6, 8 and 9) controlled panicle development (PnN or PnL), and in Group V, three regions (chrs. 1, 2 and 3) regulated tillering (PnN or TN). Characterizing these 19 genomic regions provided a detailed analysis of rice plant architecture with emphasis on the multiple effect and environmental responsive regions.

Genetic Markers↗
PubMed2004

Microbial community structure and oxidative enzyme activity in nitrogen-amended north temperate forest soils.

Large regions of temperate forest are subject to elevated atmospheric nitrogen (N) deposition which can affect soil organic matter dynamics by altering mass loss rates, soil respiration, and dissolved organic matter production. At present there is no general model that links these responses to changes in the organization and operation of microbial decomposer communities. Toward that end, we studied the response of litter and soil microbial communities to high levels of N amendment (30 and 80 kg ha(-1) yr(-1)) in three types of northern temperate forest: sugar maple/basswood (SMBW), sugar maple/red oak (SMRO), and white oak/black oak (WOBO). We measured the activity of extracellular enzymes (EEA) involved directly in the oxidation of lignin and humus (phenol oxidase, peroxidase), and indirectly, through the production of hydrogen peroxide (glucose oxidase, glyoxal oxidase). Community composition was analyzed by extracting and quantifying phospholipid fatty acids (PLFA) from soils. Litter EEA responses at SMBW sites diverged from those at oak-bearing sites (SMRO, BOWO), but the changes were not statistically significant. For soil, EEA responses were consistent across forests types: phenol oxidase and peroxidase activities declined as a function of N dose (33-73% and 5-41%, respectively, depending on forest type); glucose oxidase and glyoxal oxidase activities increased (200-400% and 150-300%, respectively, depending on forest type). Principal component analysis (PCA) ordinated forest types and treatment responses along two axes; factor 1 (44% of variance) was associated with phenol oxidase and peroxidase activities, factor 2 (31%) with glucose oxidase. Microbial biomass did not respond to N treatment, but nine of the 23 PLFA that formed >1 mol% of total biomass showed statistically significant treatment responses. PCA ordinated forest types and treatment responses along three axes (36%, 26%, 12% of variance). EEA factors 1 and 2 correlated negatively with PLFA factor 1 ( r = -0.20 and -0.35, respectively, n = 108) and positively with PLFA factor 3 ( r = +0.36 and +0.20, respectively, n = 108). In general, EEA responses were more strongly tied to changes in bacterial PLFA than to changes in fungal PLFA. Collectively, our data suggests that N inhibition of oxidative activity involves more than the repression of ligninase expression by white-rot basidiomycetes.

Analysis of Variance↗
PubMed2006

Nitrogen fixation and leaching of biological soil crust communities in mesic temperate soils.

Biological soil crust is composed of lichens, cyanobacteria, green algae, mosses, and fungi. Although crusts are a dominant source of nitrogen (N) in arid ecosystems, this study is among the first to demonstrate their contribution to N availability in xeric temperate habitats. The study site is located in Lucas County of Northwest Ohio. Using an acetylene reduction technique, we demonstrated potential N fixation for these crusts covering sandy, acidic, low N soil. Similar fixation rates were observed for crust whether dominated by moss, lichen, or bare soil. N inputs from biological crusts in northwestern Ohio are comparable to those in arid regions, but contribute substantially less N than by atmospheric deposition. Nitrate and ammonium leaching from the crust layer were quantified using ion exchange resin bags inserted within intact soil cores at 4 cm depth. Leaching of ammonium was greater and nitrate less in lichen than moss crusts or bare soil, and was less than that deposited from atmospheric sources. Therefore, biological crusts in these mesic, temperate soils may be immobilizing excess ammonium and nitrate that would otherwise be leached through the sandy soil. Moreover, automated monitoring of microclimate in the surface 7 cm of soil suggests that moisture and temperature fluctuations in soil are moderated under crust compared to bare soil without crust. We conclude that biological crusts in northwestern Ohio contribute potential N fixation, reduce N leaching, and moderate soil microclimate.

Biodegradation, Environmental↗
PubMed2001

Temperature compensation of postsynaptic currents from the extraocular muscle of temperate teleost fishes.

Miniature end plate currents were recorded from white inferior oblique extraocular muscle fibres of one temperate marine teleost (Aldrichetta forsteri, Family Mugilidae) and two temperate freshwater teleosts (Galaxias fasciatus, Family Galaxiidae and Oncorhynchus mykiss, Family Salmonidae). Miniature end plate currents were digitised and averaged over a temperature range of 5-25 degrees C. For each species, decay of miniature end plate currents was exponential and exhibited a strong temperature dependence. Lower temperatures resulted in prolonged decay phases, which decreased exponentially as a function of absolute temperature. Although values of the exponential time constant tau (tau) obtained for each species at 5 degrees C, 15 degrees C and 25 degrees C were significantly different, at any given temperature, there were no significant differences between tau values for the three species, despite differences in phylogeny (different families) and habitat (marine versus freshwater). At their normal temperature of 15 degrees C, mean values of tau for the three species ranged from 840 micros to 940 micros, and apparent activation energies ranged from -41 kJ mol(-1) deg(-1) to 50 kJ mol(-1) deg(-1). These observations confirm earlier reports that teleost miniature end plate currents are consistently shorter than those of other vertebrates.

Adaptation, Physiological↗
PubMed2003

Transcriptional analysis of the genetic elements involved in the lysogeny/lysis switch in the temperate lactococcal bacteriophage phiLC3, and identification of the Cro-like protein ORF76.

A transcriptional analysis of the lysogeny-related genes of the temperate bacteriophage Lactococcus lactis phiLC3 was performed using Northern blot hybridization during lysogeny and lytic infection by the phage. The lysogeny-related gene cluster was found to contain four promoters (P(1), P(2), Pint and P(173)), while the P(87) promoter directed transcription of orf80 and the putative gene orf87, which are located between the integrase gene and the cell lysis genes. The start sites of the transcripts were determined by primer extension. The divergently oriented lysogenic P(1) and lytic P(2) promoters located in the genetic switch region are responsible for transcription of orf286 which encodes the phage repressor, and the genes orf63 - orf76 - orf236 - orf110 - orf82 - orf57, respectively, while orf173 is transcribed from P(173). orf76 was identified as the gene encoding the Cro-like protein of phiLC3, and it was shown that ORF76 is able to bind specifically to the genetic switch region, albeit with lower affinity than does the phage repressor ORF286. ORF76 also competed with ORF286 for binding to this region. The functionality of P(1) and P(2), and their regulation by ORF286 and ORF76, was investigated using a reporter gene. In general, P(2) was a stronger promoter than P(1), but expression from both promoters, especially P(2), was regulated and modulated by flanking sequences and the presence of orf286 and orf76. ORF286 and ORF76 were both able to repress transcription from P(1) and P(2), while ORF286 was able to stimulate its own synthesis by tenfold. This work reveals the complex interplay between the regulatory elements that control the genetic switch between lysis and lysogeny in phiLC3 and other temperate phages of Lactococcus.

Bacteriophages↗
PubMed2001

Analysis of a regulator involved in the genetic switch between lysis and lysogeny of the temperate Lactococcus lactis phage phi LC3.

Sequencing of a 1.3-kb fragment of DNA from the temperate Lactococceus lactis subsp. cremoris phage phiLC3 revealed a pair of two divergently oriented ORFs, orf63 and orf286. The deduced amino acid sequence of the product of orf286 showed extensive homology to those of repressors of the temperate lactococcal phages rlt, Tuc2009 and BK5-T. A mutant with an amber mutation in orf286 gave rise to a clear plaque phenotype, indicating that this gene is involved in the lytic and lysogenic development of phiLC3. Gel mobility shift assays showed that the partially purified Orf286 protein bound specifically to the 224-bp intergenic region located between orf286 and orf63, and further characterization by DNase I footprinting analysis revealed that Orf286 protects two distinct sites within this region. Sequence analysis of the intergenic region revealed two putative, divergently oriented promoters, P1 and P2; orf286 and orf63 are probably transcribed from P1 and P2, respectively. In vivo analyses of P1 and P2 using beta-galactosidase as a reporter enzyme in L. lactis showed that transcription from P1 was repressed while transcription from P2 was stimulated in the presence of the Orf286 protein. These results suggest a complex role for the Orf286 protein in regulating the genetic switch between lytic and lysogenic growth of phiLC3.

Bacteriolysis↗
PubMed2005

Phenology of temperate trees in tropical climates.

Several North American broad-leaved tree species range from the northern United States at approximately 47 degrees N to moist tropical montane forests in Mexico and Central America at 15-20 degrees N. Along this gradient the average minimum temperatures of the coldest month (T (Jan)), which characterize annual variation in temperature, increase from -10 to 12 degrees C and tree phenology changes from deciduous to leaf-exchanging or evergreen in the southern range with a year-long growing season. Between 30 and 45 degrees N, the time of bud break is highly correlated with T (Jan) and bud break can be reliably predicted for the week in which mean minimum temperature rises to 7 degrees C. Temperature-dependent deciduous phenology-and hence the validity of temperature-driven phenology models-terminates in southern North America near 30 degrees N, where T (Jan)>7 degrees C enables growth of tropical trees and cultivation of frost-sensitive citrus fruits. In tropical climates most temperate broad-leaved species exchange old for new leaves within a few weeks in January-February, i.e., their phenology becomes similar to that of tropical leaf-exchanging species. Leaf buds of the southern ecotypes of these temperate species are therefore not winter-dormant and have no chilling requirement. As in many tropical trees, bud break of Celtis, Quercus and Fagus growing in warm climates is induced in early spring by increasing daylength. In tropical climates vegetative phenology is determined mainly by leaf longevity, seasonal variation in water stress and day length. As water stress during the dry season varies widely with soil water storage, climate-driven models cannot predict tree phenology in the tropics and tropical tree phenology does not constitute a useful indicator of global warming.

Greenhouse Effect↗
PubMed2005

A new small temperate DNA phage BcP15 isolated from Burkholderia cepacia DR11.

A Burkholderia cepacia DR11 strain was isolated during the survey of microorganisms from coastal water of deltaic Sunderbans. This strain always released temperate phage BcP15 into culture supernatant. UV irradiation of the strain also induced phage induction. The phage titer was 2.3 x 10(8). New temperate phage BcP15 has unusual structure. It has a hexagonal head, 65 nm in diameter and a tail 200 nm long, attached with single thick wavy tail fiber (424-705 nm). Phage DNA is double stranded 11.9 kb long. Southern hybridization result indicated that the phage DNA was in lysogenic state into the B. cepacia DR11 genome. SDS-PAGE of phage protein showed two major bands of molecular weight 20 kDa and 40 kDa.

Bacteriophages↗
PubMed2006

New temperate DNA phage BcP15 acts as a drug resistance vector.

This study was designed to determine the role of a new temperate DNA phage BcP15 in relation to drug resistance. The multidrug resistant Shigella flexneri NK1925 was isolated from a patient of Infectious Diseases Hospital, Kolkata, India. This strain contained five plasmids ranging in size from 3 to 212 kb. After curing of five plasmids, this strain became sensitive to antibiotics. A plasmidless multidrug-resistant strain Burkholderia cepacia DR11 was isolated during the survey of microorganisms from coastal waters of deltaic Sunderbans. This strain always released a temperate phage BcP15 into culture supernatant. Turbid plaque formation was observed on the lawn of a plasmidless version (Pl(-)35) of Shigella flexneri NK1925. A few distinct clones (Pl(-)35R) appeared within the region of each plaque after 18 h incubation. S. flexneri NK1925, Pl(-)35, and Pl(-)35R clones showed the same PFGE band pattern of XbaI-digested chromosomal DNA. However, Pl(-)35R clones were resistant to co-trimoxazole, trimethoprim, and eryth- romycin, to which B. cepacia DR11 was also resistant. Southern hybridization results indicated that these three antibiotic resistances in Pl(-)35R clones were due to a BcP15 phage lysogen in the Pl(-)35 version of S. flexneri NK1925.

Anti-Bacterial Agents↗