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The hypermineralization of diseased root surfaces.

Root surfaces which have lost their attachment due to chronic inflammatory periodontal disease present significant increases in calcium, magnesium, phosphorus, and fluoride. The clinical significance of this effect is not known in regards to wound healing, but it may be helpful in control of cervical sensitivity.

Adolescent

Maternal diabetes and changes in the hard tissues of primary teeth. III. A histologic and microradiographic study.

According to clinical studies, infants of diabetic mothers (IDM) seem to run a higher risk of mineralization disturbances in the enamel than normal healthy infants. In order to evaluate this observation at the histologic level, exfoliated primary incisors were collected from 74 IDM, representing two types of care during gestation and the perinatal period, and from 52 healthy children. The width of the neonatal line was evaluated in the enamel and the dentin by study of bucco-lingual undemineralized sections. Pre- and postnatal enamel was classified according to discolorations, deviations of prisms and hypoplasia. Microradiographic studies included measurements of the neonatal lines and classification of the degree of mineralization in the pre- and postnatal dental hard tissues. Irrespective of treatment, the IDM showed a significantly higher incidence of widened neonatal lines and also an increased frequency of postnatal disturbances compared to the controls. The observations are discussed against the background of the neonatal hypocalcemia reported in IDM.

Amelogenesis Imperfecta

Enamel structure in deciduous teeth from low-birth-weight infants.

Ground sections of deciduous teeth from 64 infants with birth weights below 2000 g and from 43 healthy full-term infants were examined in polarized light and by microradiography. The deciduous enamel displayed various changes, which were more frequent and pronounced in the low-birth-weight group. The main findings were diffuse areas of increased porosity and distinct subsurface lesions in the postnatal enamel. Five teeth with enamel hypoplasia from the low-birth-weight group were also examined. The hypoplasias were all located along the neonatal line. The postnatal enamel seemed to be more susceptible to disturbances in the mineralization than the prenatal enamel. The subsurface lesions showed an arrest in the very late stages of enamel maturation, which frequently occurred in the cervical deciduous enamel. Enamel hypoplasia is considered to be a result of severe neonatal hypocalcemia.

Dental Enamel

A method for histological examination of undecalcified teeth.

A method is presented for histological examination of undecalcified ground sections of tooth roots affected with periodontal disease. The roots were placed in Karnovsky's fixative overnight, postfixed in 2% buffered osmic acid, and dehydrated in an ascending series of ethanol. The specimens were then infiltrated with propylene-oxide and Epon-Araldite resin, embedded in Epon-Araldite, and sections were prepared using a cutting and grinding system. The resulting ground sections were 8-12 microns thick. The sections were allowed to air dry at room temperature. When thoroughly dried, a coverglass was applied using resinous mounting medium DPX. The specimens were examined by phase-contrast microscopy. The method is useful for simultaneous examination of mineralized dental tissue and bacterial morphotypes covering the root surface of teeth involved with periodontal disease.

Histological Techniques

A radiographic study of rostral tooth development in young Friesian cattle.

Twenty-nine British Friesian cattle fed the same diet, slaughtered at various weights and including bulls, steers (castrated males) and females (heifers) were examined. The stage of their rostral tooth development was recorded both visually and radiographically. Insufficient animals were present in each sex and slaughter weight treatment group to allow any comparison between them. When the data for each sex were compared, no statistically significant differences were seen between the mean ages at which bulls, steers and heifers attained each stage of dental development as determined from their radiographs. The results for all 29 cattle were then combined. This combined data showed a relationship between age and those stages of tooth formation which could be differentiated by examination of the X-ray films. As most phases of tooth formation occurred within well defined limits, it is suggested that radiographs of rostral tooth development might be used to estimate the age of cattle.

Age Factors

Mandibular cheek tooth development and its relationship to age in young Friesian cattle.

The oral and intra-osseous development of each of the six manidibular cheek teeth found in the permanent dentition of cattle was studied. The animals used were all British Friesians, of varying sex, and slaughtered at different weights at ages between 13 and 22 months. The various stages of development observed in each tooth together with the mean age and age range at which they were seen are recorded. Significant differences were found between the ages at which several of the developmental stages were seen in most of the teeth and indicated that tooth formation in the Friesian cattle studied tended to fall within well defined age limits.

Age Determination by Teeth