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The combination of fomocaine and diphenhydramine as active constituents of a new burn and wound gel (Herit) guarantees a remarkable antihistaminic effect. The UV-erythema model was a very informative test applied on the dorsal skin of 16 male volunteers.
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Cell extracts from Bacillus brevis (A.T.C.C. 10068), grown with various media, incorporated certain (14)C-labelled amino acids that are normally components of tyrothricin into material that was extracted by ethanol from the precipitate formed by adding acid. When this material was separated by paper and silica-gel thin-layer chromatography and paper electrophoresis (14)C was located in those regions that also contained gramicidin and tyrocidine. From a study of the properties of the system responsible for the incorporation it was deduced that non-tyrothricin materials were present. It was shown that the methods normally used to characterize tyrothricin do not adequately distinguish between tyrothricin and non-tyrothricin materials. However, a method for separating these materials was devised. This involved elution with ethanol from columns of acid alumina followed by gel filtration on Sephadex LH-20 with dimethylformamide-water solvent. The behaviour of gramicidin and tyrocidine on the Sephadex LH-20 column was examined, and it was concluded that the separation was not caused simply by gel filtration of unassociated molecules. Also, tyrocidine molecules with different amino acid compositions seemed to have different affinities for the Sephadex LH-20 column.
Six preparations (four liquid and two solid) were tested in a double-blind crossover design for their anti-bacterial effect on aerobic and anaerobic bacteria in saliva of 12 volunteers. The four liquid preparations contained either tyrothricin, hexetidine, hydrogen peroxide or ethanol and were tested against a rinse with water as control. The two solid preparations, in the form of lozenges, contained tyrothricin in doses of 4 or 10 mg and were tested against parafilm. A single rinse with either tyrothricin or hexetidine resulted in a significant reduction of aerobic and anaerobic bacteria in saliva which lasted for the whole 1 h observation period and was considerably more effective than hydrogen peroxide or ethanol. Similar results were seen with the solid preparations. The lozenge containing 10 mg tyrothricin was the most effective and was the only formulation capable of reducing oral aerobic bacterial counts by a factor of approximately 100.
17 antibiotics, with potential for topical use, were tested for their activity against the human oral flora. Concentrations (mumol/l) required to inhibit 90% of test strains are presented and drug activities are compared. The total cultivable oral flora was susceptible to the tetracyclines including tetracycline itself, minocycline, doxycycline, and oxytetracycline and to erythromycin. On the other hand, actinobolin, kanamycin, neomycin, streptomycin, spiramycin, tyrothricin, vancomycin, clindamycin, and chloramphenicol were ineffective against many of the human oral anaerobic bacteria even at high concentration. Penicillin was effective at high concentrations but could not be recommended because organisms which are not inhibited by low concentrations are penicillinase producers. Carbenicillin was effective against all organisms except Actinobacillus actinomycetemcomitans. The gram-negative organisms involved in adult periodontitis were most susceptible to the tetracyclines, tyrothricin, carbenicillin and clindamycin, while those associated with localized juvenile periodontitis were susceptible to the tetracyclines or erythromycin. These data, combined with the previous findings that some tetracyclines exhibit marked substantivity and collagenase inhibition activity, indicate that tetracycline or minocycline are likely to be good choices in the treatment or prevention of oral diseases.
Okuda, Kiyoshi (Department of Biochemistry and Biophysics, University of Hawaii, Honolulu), Gordon C. Edwards, and Theodore Winnick. Biosynthesis of gramicidin and tyrocidine in the Dubos strain of Bacillus brevis. I. Experiments with growing cultures. J. Bacteriol. 85:329-338. 1963.-A simple chromatographic method was developed for the isolation of gramicidin and tyrocidine from tyrothricin of Bacillus brevis. A Tryptone-yeast extract-glucose medium containing mineral salts gave the best yields of peptides in a stationary culture of the organism. The incorporation of suitable C(14)-labeled amino acids into gramicidin and tyrocidine was studied Several analogues of tyrocidine amino acids (beta-hydroxyglutamic acid, pipecolic acid, beta-2-thienylalanine, p-fluorophenylalanine, and phenyl glycine) selectively reduced tyrocidine synthesis, when added to the nutrient medium. At the same time, the production of gramicidin was augmented. Growth and protein synthesis were not affected. Two analogues in isotopic form, beta-2-thienylalanine and isoleucine, were shown to give rise to high degrees of labeling in tyrocidine.
Forty-eight infants with seborrhea capitis were treated with a preparation containing a polypeptide-fatty acid complex and tyrothricin in a propylene glycol vehicle, to determine if this material was non-toxic and if it was effective in the treatment of infantile seborrheic dermatitis. In this uncontrolled study, the preparation was effective in 45 of 48 infants so treated; a possible mild sensitivity reaction was observed in one infant.
Mass spectra of several model oligopeptides (substance P, [Arg8]-vasopressin, tyrothricin, and the B-chain of bovine insulin) have been obtained with a matrix-assisted laser desorption/ionization (MALDI) source and a custom-designed Fourier-transform mass spectrometer (FTMS). The MALDI source is outside the magnetic field in a separately pumped external chamber, and ions are injected through the fringing fields of the magnet and into the FTMS analyzer cell by a long quadrupole mass filter that is operated in the RF-only mode. A large window on the ion source housing makes it easy to point and focus the laser beam onto the sample probe tip. A good quality mass spectrum is achieved for 0.5 pmol of substance P, and the mass resolution for the B-chain of bovine insulin is 19,000.
Using 12-day old baby mice, strain NMRI, the LD50 of a Herpes simplex virus type 1 was calculated. Intragastric inoculation of the mice was carried out applying a stomach tube. Lethality of the mice was reduced from 67% to 23% by pre-incubation of the virus suspension for 30 min at 37 degrees C in the presence of 0.2 mg tyrothricin/ml. The effect was statistically significant and could be produced only after direct contact between tyrothricin and virus. Under the same conditions there was no effect of lysozyme against virus infectivity.