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Membrane filter method for the isolation and enumeration of Pseudomonas aeruginosa from swimming pools.

A membrane filter technique using black membrane filters, MacConkey agar and fluorescence under ultraviolet (UV) light was investigated for the quantitative isolation of Pseudomonas aeruginosa from swimming pools. Three thousand four hundred forty-five samples were collected from public swimming pools and enumerated by this method over a 6-month period. Fluorescent cultures were isolated from 222 specimens. Seventy-seven of these fluorescent cultures were selected for biochemical screening, with 75 (97%) being verified as P. aeruginosa. To further assess the specificity and sensitivity of this UV screening technique, a comparative study was made of some morphological and biochemical characteristics of fluorescent pseudomonads obtained from different sources. The sensitivity of the method was unimpaired by either colony types or biochemical variations of P. aeruginosa. The failure of the other two fluorescent species, P. fluorescens and P. putida, to grow and/or fluoresce on MacConkey agar at 37 C illustrates the specificity of this technique. Further studies are needed to compare the viability of P. aeruginosa on MacConkey agar to that of efficacious nonselective media.

Agar↗

A field study of some swimming-pool waters with regard to bacteria, available chlorine and redox potential.

The waters of one indoor bath and three outdoor baths were examined once an hour during 3 days (bath 1) or 6 days, for available chlorine, redox potential, permanganate number, ammonium, nitrate and total nitrogen, total bacterial count at 22 degrees C., total bacterial count at 37 degrees C. and faecal coliform bacteria. The weather, number of swimmers and the chlorine gas addition were continuously registered, and the pH was checked a few times at each bath. In bath 1, an indoor pool with aluminium sulphate precipitation about once a week and with sand filters back-washed every 2 days, less than 10 bacteria/ml. were found in all samples. In bath 2, an outdoor pool with aluminium sulphate precipitation twice a week and with sand filters back-washed twice a week, also few bacteria were found. In bath 3, an outdoor pool with only filtering through sand filters back-washed about every 14 days, high bacterial counts were found every day except the first, when the filters had been newly back-washed. In bath 4, an outdoor pool with only filtering through sand filters back-washed about once a week, high bacterial counts were found now and then during the first 4 days when the weather was warm, but few bacteria were found the last 2 days when the weather was cold and windy, and there were few swimmers.Values from different analyses on the same sample showed relatively good correlation between the redox potential and the free available chlorine. In bath 3 both the redox potential and the available chlorine were weakly correlated to the bacterial count, but in bath 4 there was no such correlation. No other factors were well correlated with the bacterial count either.The bacterial counts at 22 degrees and 37 degrees C. were of the same order. No faecal coliforms were ever found. Use of these bacteria as indicator organism in swimming pools is criticized.The method of using certain minimum values of the free available chlorine as guarantee for a satisfactory bacteriological quality of the swimming pool water is also questioned. The degree of purity of the water is fundamentally connected with the disinfecting power of the available chlorine.Use of certain minimum values of the redox potential, according to these investigations, seems to be a method of somewhat greater accuracy. Provided that the methods of precipitation are performed correctly and filters are being back-washed often enough, then an automatically registering redox potential device, perhaps connected to the chlorine gas pump, ought to constitute a good control of the hygienic quality of a swimming-pool water. This must, however, always be completed by bacteriological examinations, preferably made at high bathing load.

Bacteria↗

[Green hair caused by frequent swimming pool use].

Three patients presented with an acquired green discoloration of their scalp hair. History revealed that all of them swam regularly in private swimming pools. Examination of the hair by atomic emission spectroscopy showed that the green discoloration was caused by an excessively high copper content of the hair. This exogeneous discoloration is characteristically related to the uptake of copper from private swimming pools.

Adolescent↗

[Amoeba of the Acanthamoeba species in swimming pools and lakes of the Lublin area and in people using contact lenses].

Examinations of water for the presence of Acanthamoeba in covered and open air swimming pools and lakes of the city of Lublin were performed in order to determine the places where the invasion of amoebas of Acanthamoeba kind on the eyes can occur. Pathogenic strains of Acanthamoeba were found in all collected water samples. The cultures from the conjunctival sac of regular swimmers practising every day in these pools were taken. Amoebas were found in none of the examined cultures. The observations have shown that contact lenses should be protected from the contamination with swimming pool water. Amoebas were not revealed in the conjunctival sac of the swimmers after bathing.

Adult↗

[Amoebae in swimming-pools: microbiological and epidemiological aspects (author's transl)].

An inquiry was held to determine the occurrence and quantitative importance of "Limax" amoebae in 8 swimming-pools of the North of France. The influence of water flow system, filters and water disinfectants was studied. The connections with the bacterial flora growing at 37 degrees C and the presence of strains able to grow at 41 degrees were analyzed. The significance of their presence in the swimming-pools is discussed from the obtained results.

Amebiasis↗

Interaction between piloting and beacon homing by rats in a swimming pool.

In three experiments, rats in a swimming pool were trained to find a submerged platform with a beacon attached to it. For some rats this beacon unambiguously identified the location of the platform; for others the beacon was made ambiguous by placement of an identical beacon in a different part of the pool. Test trials, in the absence of the platform and the beacons, revealed more persistent searching near the original location of the platform if the beacon attached to the platform had been ambiguous. These results show that learning about the location of the platform, with regard to cues that lie beyond the pool, is influenced by the extent to which an animal can find the platform by relying on other cues. The final experiment shows that this interaction between cues is influenced by an animal's prior experience.

Animals↗

Thirty-six consecutive under 5 year old domestic swimming pool drownings.

Data were collected by the local nurse on each of the 36 drownings in domestic swimming pools of under 5 year olds that occurred between 1 September 1982 and 31 December 1986. The likely effect of New Zealand's 1987, Fencing of Swimming Pools Act in preventing these drowings, had it been in effect, is considered. It is concluded that at least 29 (80%) of the 36 drownings would have been prevented. Examination of the circumstances of the drownings leads to the conclusion that certain alternatives to the fencing law that have been proposed, such as teaching water skills to infants, fencing property boundaries, and use of pool covers, are unlikely to have been as effective.

Child, Preschool↗

Health effects of attending a public swimming pool: follow up of a cohort of pupils in Paris.

OBJECTIVES: This study aimed to determine the health effects of attending a well-kept school swimming pool maintained according to French public health regulations. METHODS: This prospective month long study was carried out on a randomised sample of pupils aged 5 to 18 years who attended a private French school with two swimming pools. The children surveyed, helped by their parents, had to fill in questionnaires about their bathing habits and symptoms during the survey period. Inspections of the pool complex were made and these included physicochemical and bacteriological analyses of the pools' water. PARTICIPATION: The response rates achieved were 70% at primary and middle school levels but only 25% in the high school pupils. Because of this older teenagers were excluded from the final analysis (of 246 children). RESULTS: Compared with non-bathers, bathers experienced fatigue and eye irritation significantly more often (p < 0.001). The eyes were red (38% of bathers) and/or watery (16%) after swimming but this resolved spontaneously within 24 hours. Bathing behaviour (bath duration, head immersion, wearing swimming goggles) did not affect these incidence rates noticeably. There were no differences between bathers and non-bathers with regard to other symptoms, especially otolaryngological ones. This survey does not allow definite conclusions to be made about verrucas because 22% of non-bathers were exempted from swimming because of verrucas that they might have caught previously in a pool. CONCLUSIONS: Except for verrucas, the methodology was adequate and daily self reporting of symptoms was feasible. This college largely recruits pupils from higher social classes and is not therefore representative of schools in Paris.

Adolescent↗

Follicular dermatitis outbreak caused by Pseudomonas aeruginosa associated with a motel's indoor swimming pool.

Fourteen cases of pustular dermatitis occurred in members of a snowmobile club who swam in a motel pool in West Yellowstone, Mont., in February 1978. Survey questionnaires identified seven additional cases in guests at the motel the same weekend. All those with rashes had used the swimming pool and dry sauna on February 17 or 18. Among 56 survey respondents, swimming pool and sauna use were significantly associated with illness (P = .0002 by Fisher's exact test). Pseudomonas aeruginosa serotype 0:11 was isolated from a pustule on the skin of one club member and from four samples of the indoor-outdoor carpet that surrounds the pool. A specific precipitating event for the outbreak was not identified, but disinfection practices at this facility (a single daily chlorination, no measurement of chlorine levels, toleration of grossly cloudy water, soggy poolside carpet) may have established conditions in which P. aeruginosa could grow intermittently and cause disease. These cases are the first documented outbreak of P. aeruginosa dermatitis in which a whirlpool bath has not been implicated.

Hair Diseases↗

A community outbreak of Cryptosporidium infection associated with a swimming pool complex.

A case-control study was conducted to investigate the cause of a sudden increase in cases of cryptosporidiosis notified to the Brisbane Southside Public Health Unit from January to March 1998. Fifty-two eligible cases were identified over a three-week period early in 1998. Thirty-one of these cases and 21 control subjects participated in the study. Swimming in the 2 weeks before onset of illness was identified as a likely risk factor for cryptosporidiosis infection (OR 3.1, CI 0.8-12.6, P = 0.06). Analysis of swimming pool attendance identified swimming at Pool Complex A as a significant risk factor for the acquisition of cryptosporidiosis (OR 8.9, CI 1.5-67.4, P = 0.004). No other potential risk factors were significantly associated with illness. The detection of cryptosporidium oocysts in three of the four pools at Pool Complex A supported the findings of the case-control study. As a response to this outbreak, Queensland Health has developed a Code of Practice outlining measures for the control and prevention of future outbreaks of swimming pool-associated cryptosporidiosis and/or giardiasis.

Adolescent↗

Acute inhalation injury with evidence of diffuse bronchiolitis following chlorine gas exposure at a swimming pool.

A previously healthy 23-year-old man with nonproductive cough and sore throat presented to the hospital a few hours after chlorine gas exposure at a fitness center swimming pool. Initial physical examination and chest radiograph were normal. Thirty-six hours later he developed worsening dyspnea and cough, with development of blood-tinged sputum. Arterial blood gas analysis showed mild hypoxemia and a subsequent chest radiograph demonstrated diffuse tiny nodular opacities. Findings on a thin-section computed tomogram of the chest were consistent with diffuse bronchiolitis. Pulmonary function tests showed a mild obstructive abnormality and he demonstrated substantial bronchodilator response. The patient was treated with oral corticosteroids and an inhaled beta(2) agonist, to which he responded well, with full clinical recovery occurring over 5 months. This manifestation of chlorine gas exposure at a swimming pool is unusual.

Acute Disease↗

Evaluation of disinfectant efficacy against biofilm and suspended bacteria in a laboratory swimming pool model.

Laboratory reactor systems designed to model specific environments enable researchers to explore environmental dynamics in a more controlled manner. This paper describes the design and operation of a reactor system built to model a swimming pool in the laboratory. The model included relevant engineering parameters such as filter loading and turn-overs per day. The water chemistry in the system's bulk water was balanced according to standard recommendations and the system was challenged with a bacterial load and synthetic bather insult, formulated to represent urine and perspiration. The laboratory model was then used to evaluate the efficacy of six chemical treatments against biofilm and planktonic bacteria. Results showed that the biofilm was able to accumulate on coupons and in the filter systems of reactors treated with either 1-3 mg/L free chlorine or 10 mg/L polyhexamethylene biguanide (PHMB). All the treatments tested resulted in at least a 4 log reduction in biofilm density when compared to the control, but shock treatments were the most effective at controlling biofilm accumulation. A once weekly shock dose of 10 mg/L free chlorine resulted in the greatest log reduction in biofilm density. The research demonstrated the importance of studying a biofilm in addition to the planktonic bacteria to assess the microbial dynamics that exist in a swimming pool model.

Bacteria↗

The fate of chlorine and organic materials in swimming pools.

The fate of organic nitrogen and carbon introduced into a swimming pool by pool users has been studied using a 2.2 m(3) model pool. The study made use of a body fluid analogue (BFA), containing the primary endogenous organic amino compounds, and a soiling analogue represented by humic acid (HA). The system was used to examine the effect of organic loading and organic carbon (OC) sources (i.e. amino or HA) on the levels and speciation of the key chlorinated disinfection by-products of trihalomethanes (THMs) and chloramines under operating conditions representative of those employed on a full-scale pool. Results revealed OC, chloramines and THMs to all attain steady-state levels after 200-500 h of operation, reflecting mineralisation of the dosed OC. Steady-state levels of OC were roughly linearly dependent on dose rate over the range of operational conditions investigated and, as with the chloramine levels recorded, were in reasonable agreement with those reported for full-scale pools. THM levels recorded were somewhat lower than those found in real pools, and were dependent on both on pH carbon source: the THM formation propensity for the soling analogue was around eight times than of the BFA. Of the assayed by-products, only nitrate was found to accumulate, accounting for 4-28% of the dosed amino nitrogen. Contrary to previous postulations based on the application of Henry's Law, only insignificant amounts of the volatile by-products were found to be lost to the atmosphere.

Chlorine Compounds↗

Fungi in two swimming pools in Assiut town, Egypt.

50 water samples from 2 public swimming pools were tested for the presence of fungi using the hair baiting technique and plate count methods. Sabouraud's-dextrose agar and Littman-oxgall agar media were used for isolation of the fungi. Of the keratinolytic fungi 8 species were recorded of which three species were dermatophytes (Trichophyton terrestre, 14% of the samples; T. mentagrophytes, 10% and Microsporum gypseum, 6%). On Littman-oxgall agar the following species were recovered: Aspergillus flavus, A. fumigatus, A. niger, Penicillium chrysogeman, Syncephalastrum racemosum, Alternaria alternata and Mucor hiemalis.

Arthrodermataceae↗

Plasma chloroform concentrations in swimmers using indoor swimming pools.

This study evaluated swimmers and visitors who were exposed to chloroform (CHCl3) at three indoor swimming pools in Modena, Italy. Chloroform was measured in plasma samples of 127 subjects present at the pools and in 40 nonexposed subjects. The analyses were performed by head-space gas chromatography. Chloroform was present in all samples collected from the 127 subjects who attended the pools (median = 7.5 nmol/l; range = 0.8-25.1 nmol/l). Agonistic swimmers who trained for competitions showed a significantly higher mean value of plasma CHCl3 than nonagonistic swimmers and visitors. Plasma CHCl3 levels were significantly correlated with (a) CHCl3 concentrations in water and in environmental air, (b) the number of swimmers in the pools, and (c) the time spent swimming. Covariance analysis showed that plasma CHCl3 levels also depended on the intensity of the sport activity (total explained variance = 67.4%).

Air Pollutants↗