[Regeneration and budding in Salmacina (Annelida, Polychaeta)].
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Nereis garwoodi n. sp. is described on the basis of eight syntype specimens (six atokous and two heteronereis) collected in Bahía Chetumal, Mexican Caribbean coast, and the variability in the paragnath numbers in the pharynx is established using 180 specimens; paragnath numbers are I:10(SD = 1.9); II:30 (SD = 2.6); III:41 (SD = 5.2); IV:29 (SD = 3.5), V:1, VI:4, VII-VIII: > 30. Its eyes are big and its longest tentacular cirri reaches setiger 11. A revised key to species of Nereis recorded from the Grand Caribbean Sea is included.
Prototroch formation was studied in the polychaete Nereis virens using light, scanning electron, and confocal laser microscopy. Cell lineage of trochoblasts was followed and chronology of their appearance was determined. The prototroch ciliary ring is formed by twelve descendants of micromere lm(2). The remaining four primary trochoblasts have no cilia and, together with descendants of accessory trochoblasts, become anterior supporting cells of the prototroch. Posterior supporting cells are formed by secondary trochoblasts, which are derived from the second micromere quartet 2m. The results obtained make it possible to analyze one of the ancient programs of animal development.
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The reproduction of Nereid Perinereis cultrifera in the English Channel and the Atlantic is of an epitokous type. The same Nereid in the Mediterranean (Bay of Alger) has been described as atokous (Marcel, 1962) and since that time epitoky in mediterranean specimens has been considered to be an accidental phenomenon. However, in the present study polychaetes collected at Salammbô (near Tunis) show that those P. cultrifera reproduce exclusively by epitoky.
The presence in the marine worm Nereis diversicolor of a low molecular mass protein with the capacity to bind cadmium has been previously demonstrated. Poly(A)(+)-mRNA were extracted from coelomocytes of Nereis diversicolor and were translated either in vitro, using a rabbit reticulocyte lysate, or in vivo into Xenopus laevis oocytes. Analysis of synthesized polypeptides by enzyme-linked immunosorbent assay (ELISA) and by Western blotting, using a specific monoclonal anti-MP II antibody, showed that this metalloprotein was translated both in in vitro and in vivo translation systems, with an apparent molecular mass of 11-13 kDa. Two other products, with 26.5 and 28 kDa molecular mass, cross-reacted with the monoclonal anti-MP II antibodies. The present work confirms that coelomocytes are sites of important synthesis of MP II-mRNA.
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High specific and nonspecific cholinesterase activities have been demonstrated in the whole nervous system of Nereis. These might reveal the existence of a cholinergic nervous system whose control would be exerted in different sites (central nervous centers, muscular junctions...).
Meiotic maturation is triggered by ionophores A 23187 and X 537 A, even in absence of extracellular calcium. CaMg free medium alone initiates 100% maturations while Ca free SW triggers varying percentages of maturation and Mg free SW has no effect.