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A satellite DNA of the Sparidae family (pisces, perciformes) associated with telomeric sequences.

This paper reports on the isolation and localization of the subtelomeric DraI satellite DNA in the Sparidae family. Gene cloning determined that the DraI satellite DNA is present in only 3 species (Pagrus pagrus, P. auriga, and Pagellus erythrinus) of the 10 Sparidae species analyzed. The results were confirmed by PCR amplification. This satellite DNA is located in a subtelomeric position in all 48 acrocentric chromosomes of these species. However, interstitial loci are also observed. Sequence analysis of monomers of this repetitive family indicates that the satellite DNA is associated with telomeric sequences, (TTAGGG)n, in at least one species, P. erythrinus. This is the first direct demonstration of the existence of the consensus telomere sequences of vertebrates in fish. Likewise, this report also demonstrates that the ends of fish chromosomes have a structure similar to those of most eukaryote chromosomes, viz., telomere sequences and subtelomeric sequences associated by a boundary in which both types of sequences are interspersed. The recent origin of the DraI satellite DNA and its use as a phylogenetic marker is discussed.

Animals↗

The chromosomal complement of the artedidraconid fish Histiodraco velifer (Perciformes: Notothenioidei) from Terra Nova Bay, Ross Sea.

The karyotype of Histiodraco velifer from the Antartic Ocean was analyzed using various banding methods and in situ hybridization with a telomeric probe. A male and a female had a diploid set of 46 chromosomes (6 submetacentric + 40 acrocentric, FN = 52); the nucleolar organizer was CMA3-positive and was located on the short arm of a medium-sized submetacentric pair. All chromosomes stained uniformly with DAPI, whereas C-banding revealed heterochromatic blocks that were mostly located centromerically and telomerically and were resistant to ALUI digestion. The substantial identity of the karyotype of H. velifer with that of the other artedidraconids investigated so far suggests that chromosome changes must have played a less than significant role in the speciation among the lineages of this fish family endemic to Antarctica.

Animals↗

Cytogenetic analysis of gilthead seabream Sparus aurata (Pisces, Perciformes), a deletion affecting the NOR in a hatchery stock.

We have cytogenetically characterized a hatchery stock of gilthead seabream, Sparus aurata. The study included larvae, juveniles and adults. In S. aurata (diploid chromosome number 2n = 48), a pair of NORs is located at the ends of the short arms of the first submetacentric pair of chromosomes. In this stock we discovered a polymorphism which affects the NORs, and, by means of several cytogenetic and molecular techniques, we demonstrate that this polymorphism is due to the complete deletion of one of the two NORs in a high number of individuals. The significance of these cytogenetic characteristics for this species are discussed since they may be the source of aquaculture problems.

Aging↗

The EcoRI centromeric satellite DNA of the Sparidae family (Pisces, Perciformes) contains a sequence motive common to other vertebrate centromeric satellite DNAs.

By means of cloning, sequencing, and fluorescence in situ hybridization, we have determined that the EcoRI satellite DNA family is conserved in the 10 sparid species analyzed here. Its conservation, its chromosomal location at the centromere of each chromosome, and its structural features could make this satellite DNA family an important structural and/or functional element of the centromeres of these species. Monomeric units of this satellite DNA have a consensus length of 187 bp. Its sequence is characterized by a high AT content and the presence of short runs of consecutive AT base pairs. These monomeric EcoRI repeats also contain three to four copies, depending on the species, of a short sequence reflecting the repetitive duplication and subsequent divergence of an ancestral 9-bp sequence in this family. This sequence motive is conserved in some parts of the monomeric units of the different species studied at the same positions, and, precisely, surrounding the area in which the curvature of the monomeric molecule is greatest. The 9-bp sequence motive is similar to other direct-repeat sequences of the centromeric satellite DNAs of other vertebrates, including those of amphibians and mammals.

Animals↗

Fine structure of spermatozoa in the common pandora (Pagellus erythrinus Linnaeus, 1758) (Perciformes, Sparidae).

Scanning and transmission electron microscopy were used to investigate the fine structure of the sperm of the Sparid fish Pagellus erythrinus L. The spermatozoon of pandora has a spherical head lacking an acrosome, a cone-shaped midpiece and a long tail. The midpiece houses a single mitochondrion. The centriolar complex lies inside the nuclear fossa and is composed of a proximal and a distal centriole which are arranged at right angles to each other. The flagellum is inserted medio-laterally into the head, contains the conventional 9+2 axoneme and possesses one pair of lateral fins. On the basis of its ultrastructural organization, the pandora sperm can be regarded as an evolved form of the primitive spermatozoon found in Teleosts. According to the morphological classification proposed by Mattei (1970), the sperm of pandora belongs to a "type I" designation, like that of the other Sparid fish.

Animals↗

Sex ratio and size structure of Micropogonias furnieri (Desmarest, 1823) (Perciformes, Sciaenidae) in Sepetiba Bay, Rio de Janeiro, Brazil.

Sex ratio and size structure constitute basic information in assessing reproductive potential and estimating stock size in fish populations. One hundred fifty-one individuals of Micropogonias furnieri caught by experimental otter trawls, in three zones (inner, central, and outer) of Sepetiba Bay between October 1998 and September 1999 were examined. Males outnumbered females (1.3:1.0) in all zones but no significant differences were detected. Only in the outer zone (5.0:1.0) were male/female rates significantly different according to the chi-square test. No temporal differences were observed in sex ratio. Fish size ranged from 81 to 244 mm total length (TL) with significant differences in 155 to 185 mm TL size classes, where males predominated, and a slightly higher number of females were observed for the smaller size class (TL = 95-150 mm). Size distribution varied according to the zone, with juveniles predominating in the inner and adults in the outer zone. Spatial difference in size structure observed in this study indicates that the inner bay is a rearing ground during the first life-cycle period, and movement toward the sea occurs as fish increase in size.

Animals↗

Meals for two: foraging activity of the butterflyfish Chaetodon striatus (Perciformes) in southeast Brazil.

The banded butterflyfish (Chaetodon striatus) from the tropical and subtropical western Atlantic is a territorial, diurnal forager on benthic invertebrates. It is usually seen moving singly or in pairs, a few meters above the sea floor. We studied the foraging activity of C. striatus on rocky reefs in southeastern Brazil. This fish spent about 11 h and 30 min per day on feeding activities, and preferred colonies of non-scleratinian anthozoans over sandy and rocky substrata while foraging. The lowest feeding rates were recorded in the early morning and late afternoon, but we found no further differences between feeding rates throughout the day. We also found no differences between the feeding rates of paired and single individuals.

Animals↗

Kudoa hypoepicardialis n. sp. (Myxozoa: Kudoidae) and associated lesions from the heart of seven perciform fishes in the northern Gulf of Mexico.

Kudoa hypoepicardialis n. sp. infects the space between the epicardium and the compact myocardium and, in intense infections, the pericardial chamber of man-of-war fish (Nomeus gronovii) (Nomeidae) (the type host), blue runner (Caranx crysos) (Carangidae), Warsaw grouper (Epinephelus nigritus) (Serranidae), Atlantic tripletail (Lobotes surinamensis) (Lobotidae), northern red snapper (Lutjanus campechanus) (Lutjanidae), black drum (Pogonias cromis) (Sciaenidae), and bluefish (Pomatomus saltatrix) (Pomatomidae) in the northern Gulf of Mexico. This is the first report of a Kudoa sp. from the heart of a fish in the Gulf of Mexico, and of these hosts, only the bluefish was previously identified as a host for a species of Kudoa. Spores of the new species varied slightly in size among these hosts but were regarded as conspecific based on their nearly identical (99.9%) small-subunit (SSU) ribosomal DNA (rDNA) sequence. The new species differs both from the 4 nominal species of Kudoa reported from fishes in the Gulf of Mexico and from K. pericardialis, an allopatric species that infects the pericardial cavity, by the combination of having a large spore, a small polar capsule, and a polar filament with a single coil. The new species is morphologically and genetically most similar to K. shiomitsui, an allopatric species that infects the heart and pericardial cavity, but is distinguished from it based on a 4.2% difference in the SSU rDNA sequence. Heart lesions primarily were restricted to the vicinity of plasmodia and included a layer of fibrinous inflammation characterized by lymphocytes, macrophages, and granulomas as well as epithelioid encapsulations around plasmodia. Heavily infected hosts had melanin-like deposits and adipose cells beneath the epicardium. and the epicardium was discontinuous and apparently breached by plasmodia in some regions. Cardiac muscle, gill, liver, spleen, intestine, and kidney were normal.

Animals↗

Karyakartia egyptensis n. sp. (Digenea: Lepocreadiidae) from the Jarbua terapon, Terapon jarbua (Forsskål) (Perciformes: Terapontidae), from the Red Sea and emendation of Karyakartia.

Karyakartia egyptensis n. sp. (Digenea: Lepocreadiidae: Lepocreadiinae) is described from the intestine of the Jarbua terapon, Terapon jarbua (Forsskål), collected from the Red Sea off the coast of Hurghada, Egypt. Karyakartia egyptensis n. sp. differs from K. pambanense in that the former species possesses larger spiniform structures around the perimeter of the mouth (48-54 microm as compared with 25-30 microm), a uroproct rather than ceca that end blindly or form a cyclocoel, and a somewhat smaller egg (60-68 microm as compared with 70-73 microm).

Animals↗

A new cystidicolid nematode from Mullus surmuletus (Perciformes: Mullidae) from the western Mediterranean.

Ascarophis valentina n. sp. is described from Mullus surmuletus off the Valencian coast of Spain on the basis of both light and scanning electron microscopy. It can be distinguished from the other members of the genus by the length of the left (long) spicule of the males and by egg morphology. An updated grouping of the species of Ascarophis considered valid is provided with respect to these characters. The new species resembles Ascarophis capelanus, belonging to the group of species possessing eggs with a single polar knob with filaments, but is distinguished by the size of the body, the length of the esophagus (especially in relation to body length), the position of the vulva, and the size of the left spicule. The new species also shows substantial morphological differences compared with the 3 species, Ascarophis mullusi, Ascarophis upenei, and Ascarophis parupenei, previously described from mullid hosts.

Animals↗

Neotropical Monogenoidea: Euryhaliotrema dontykoleos n. sp. (Dactylogyridae) from the gills of the freshwater sciaenid, Pachyurus junki (Perciformes).

Euryhaliotrema dontykoleos n. sp. (Monogenoidea, Polyonchoinea, Dactylogyridae) is described based on specimens collected from the gills of Pachyurus junki (Teleostei, Sciaenidae, Pachyurinae) in the Rio Tocantins and its tributaries, State of Tocantins, Brazil. The new species, the first species of Monogenoidea reported from a pachyurine, is easily differentiated from its congener by the presence of toothlike projections in the border of the vaginal opening and by having a trapezoidal anteromedial projection on the ventral bar. Phylogenetic analysis indicates that the new species is not a member of the clade that includes the Euryhaliotrema spp. from Plagioscion spp., a second lineage of freshwater sciaenids in South America. Euryhaliotrema dontykoleos shares an immediate ancestor with the clade formed by parasites infecting marine Paralonchurus spp., (Sciaenidae), and those of Plagioscion spp.

Animals↗

Neotropical Monogenoidea. 43. Diplectanum monticellii n. sp. (Diplectanidae) from the gills of Cynoscion leiarchus (Perciformes: Sciaenidae) in Brazil.

Diplectanum monticellii n. sp. is described from the gills of Cynoscion leiarchus, a marine Sciaenidae, from Itacuruçá, Rio de Janeiro (type locality); Baía de Guaratuba, Paraná; and Pontal do Sul, Paraná in Brazil. The new species is characterized by the following features: bell-shaped male copulatory organ with a sleevelike base, accessory piece absent, vas deferens looping left intestinal cecum, vaginal aperture sinistroventral, and ventral anchor with elongate superficial and deep roots.

Animals↗

Intraovarian cavity leucocytes of viviparous fish, Neoditrema ransonneti (Perciformes, Embiotocidae).

To obtain basic information on the properties of the intraovarian cavity leucocytes (IOCLs) of the viviparous teleost, Neoditrema ransonneti, morphological characteristics and numerical changes of IOCLs during the reproductive cycle were investigated. In the ovaries of newborn females, leucocytes exuded into the lumen were observed first in November, prior to insemination of semen. These cells were primarily macrophages, neutrophils and lymphocytes. Among them, macrophages were invariably the largest population throughout the reproductive cycle. They began to phagocytize spermatozoa in December, when spermatozoa were first detected in the ovary. The number of IOCLs gradually increased from November in the newborn female. However, this increase is not ascribed to the effect of copulation or the presence of semen, because the number of leucocytes also increased in non-mating fish. While developing embryos were discharged into the ovarian lumen at the latest in January, a number of spermatozoa and spermatozoa-phagocytizing macrophages were seen until March. Even after the extinction of sperm cells, numerous IOCLs remained in the lumen and coexisted with fetuses until their parturition. These results suggest that IOCLs play roles in successful pregnancy, besides elimination of remaining spermatozoa.

Analysis of Variance↗

Evaluation of Melanotaenia duboulayi (Atheriniformes: Melanotaeniidae), Hypseleotris galli (Perciformes: Eleotridae), and larvicide VectoLex WG (Bacillus sphaericus) for integrated control of Culex annulirostris.

Australian freshwater fish species Melanotaenia duboulayi and Hypseleotris galii were selected for a small plot field evaluation of an integrated pest management strategy using native fish and VectoLex WG (Bacillus sphaericus) for the control of Culex annulirostris Skuse, the principal freshwater vector of arbovirus Ross River virus in Australia. When tested alone, the level of control afforded by M. duboulayi and H. galii was highly dependent on the prerelease density of mosquito larvae; and even when stocking rates as high as 10 g per pond (>30 kg/ha) were used, larval abundance was too high to attain adequate control from fish alone. In contrast, treatment with VectoLex WG at 500 g/ha resulted in 100% mortality of Cx. annulirostris immatures, but no residual activity was evident. The delayed reduction of Cx. annulirostris immatures in ponds stocked with fish alone, and the recolonization by Cx. annulirostris in ponds after treatment with B. sphaericus, did not occur when both treatments were combined.

Animals↗

[Entobdella hippoglossi (Monogenoidea: Capsalidae) from a perciform fish from the Pacific Ocean and new information on Sessilorbis limopharynx].

A monogenean Entobdella hippoglossi (Capsalidae: Entobdellinae), a common parasite of the flat fishes (Pleuronectidae), is recorded for the first time from an ocean perch Sebastes glaucus (Scorpaenidae) in the north-east part of Pacific ocean. New data on hosts and location of Sessilorbis limopharynx (Capsalidae: Trichopodinae) are also given.

Animals↗

[Feeding habits of cichlid species (Perciformes: Cichlidae) in Caobas lake, Quintana Roo, Mexico].

Feeding habits of seven cichlid species (Archocentrus octofasciatus, A. spilurus, "Cichlasoma" robertsoni, "C." synspilum, "C." urophthalmus, Petenia splendida, Thorichthys meeki) in Lake Caobas, southern Yucatan Peninsula, were studied. Samples were taken with enclosure and cast nets during the dry and rainy seasons of 1995 (day and night). The environment was characterized by measuring temperature, conductivity and pH. All individuals were below 41 mm SL (N = 281). Frequency of occurrence and prey abundance were analyzed. Main prey items were chironomids, mites, copepods, cladocerans, and ostracods. The cichlids fed mainly on zooplankton, with the partial exceptions of P. splendida (piscivore), "C." synspilum and A. spilurus (herbivores). A cluster analysis showed that the most similar trophic spectra were those of T. meeki, "C." robertsoni and "C." salvini, which were also the least diverse. "C." synspilum and A. spilurus had an intermediate distance between their diets and those of other species. The species with the most distinctive feeding composition were P. splendida (with the most diverse and equitable diet) and the omnivore A. octofasciatus (whose diet was the richest one). T. meeki showed quantitative diel, ontogenetic, and seasonal diet changes, but none between sexes. "C." robertsoni, "C." salvini and "C." synspilum differ in food habits in Caobas and in other localities, a fact that underscores the trophic adaptability of cichlids. Trophic overlap between cichlids in Caobas could imply absence of competition, perhaps because resources are abundant in the ecosystem.

Animals↗

The ultrastructure of the pre-meiotic and meiotic stages of spermatogenesis in Plagioscion squamosissimus (Teleostei, Perciformes, Sciaenidae).

Spermatogenesis of 'corvina' P. squamosissimus starts from a stem cell that gives rise to germ cells. These cells are enveloped by Sertoli cells, forming cysts. The germ cells in the cysts are all at the same stage of development and are interconnected by cytoplasmic bridges. Spermatogonia are the largest germ cells. In the cysts, these cells differentiate into primary spermatogonia and secondary spermatogonia. The primary spermatogonia are isolated in the cyst and give rise to the secondary spermatogonia. After several mitotic divisions, they produce spermatocytes I, which can be identified by synaptonemal complexes in the nucleus. The spermatocytes I enter the first phase of meiosis to produce the spermatocytes II. These are not very frequently seen because they rapidly undergo a second phase of meiosis to produce spermatids.

Animals↗

[Length, body weight and sex of the golden Coryphaena hippurus (Perciformes: Coryphaenidae), of the littoral of Sinaloa, Nayarit and Baja California Sur, Mexico].

Dolphin fish population data were recorded from landings in Sinaloa, Nayarit and Baja Califonia Sur, Mexico, in 1997. Of 3,211 organisms, only 2,812 produced complete biometric data. The maximal length and weight were 192 cm and 30 kg. The three sampling sites and the climatic periods also were associated with different size (P < 0.05). The covariate analysis of weight and length relationships indicated significative differences (P < 0.05) for total data and by sex. Condition factor was higher throughout the study period in Mazatlán, and the variation was higher, in the spring. The sexual ratio was in Mazatlán constant, while there were more males in Nayarit in the fall and the spring and in Los Cabos in the spring. There may be population differences, between the central mexican Pacific Coast and the Peninsula of Baja data.

Animals↗