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PubMedDate not supplied

Genotoxicity study in lymphocytes of offset printing workers.

The potential cytogenetic damage in offset printing workers was evaluated using sister chromatid exchanges (SCEs), chromosome aberrations (CAs) and micronuclei (MN) as biomarkers in peripheral lymphocytes of 26 volunteers (14 workers, 12 controls). The CA, SCE and MN frequency of offset printing workers was significantly higher than in their controls. The replication index (RI) was not affected while the mitotic index (MI) was affected most in the workers. It can be concluded from this study that chronic occupational exposure to printing dyes and thinner may lead to a slightly increased risk of genetic damage among offset printing workers.

Adolescent↗
PubMed2007

Microcontact printing of laminin on oxygen plasma activated substrates for the alignment and growth of Schwann cells.

Microenvironment mimicking biological situation is a vital issue in tissue regeneration. With much progress being made, one of the major challenges remains to develop a convenient method to fabricate the scaffold microenvironment suitable for cell attachment and proliferation. This article demonstrates the efficacy of microcontact printed laminin, an extracellular matrix protein, on three different oxygen plasma treatment substrates-tissue culture polystyrene, poly(methyl methacrylate) films, and chitosan films-for alignment and growth of the Schwann cells in in vitro culturing. Replica molding of polydimethylsiloxane elastomeric stamps, fabricated from patterned SU-8 structure on silicon master, was used to print laminin on the three substrates. Pattern and growth of Schwann cells for low (10(3) cells/cm(2)) and increased cell density (2 x 10(4) cells/cm(2)) on the varied substrates with and without microcontact printed laminin were characterized. Results of in vitro cell culture of Schwann cells showed a high degree of cell orientation on the laminin-micropatterned substrates for both cell densities. However, different cell seeding densities will strongly impact the morphology and orientation of Schwann cells. Microcontact printing proves to be a convenient means to pattern cell-recognition molecules on scaffold for cell-guilded growth in tissue regeneration.

Animals↗
PubMed2000

Computed radiography printing problems: a quantitative, observer-independent solution.

Even though facilities using computed radiography (CR) operate in an electronic environment, the production of hard-copy films is still necessary during the transition period, as well as for particular needs following complete implementation. We have implemented a quantitative technique to match the response of printed CR film with that of previous screen/film combinations. A stepwedge is radiographed using the conventional system. The same stepwedge is then radiographed (same geometry and technique) using the CR system. Following processing and printing, the plot of optical density versus step for the CR system is compared with that of the screen/film system. Adjustments are made to the printing parameters until the response curves are identical. All other translation tables in the system are set to be linear. This has proven to be a valuable technique for us and provides CR printed image quality that is equivalent to that of our previous screen/film combinations.

Computer Peripherals↗
PubMed2004

Inkjet printing for high-throughput cell patterning.

The adaptation of inkjet printing technology to the complex fields of tissue engineering and biomaterial development presents the potential to increase progress in these emerging technologies through the implementation of this high-throughput capability via automated processes to enable precise control and repeatability. In this paper, a method of applying high-throughput inkjet printing to control cellular attachment and proliferation by precise, automated deposition of collagen is presented. The results indicate that commercial inkjet printing technology can be used to create viable cellular patterns with a resolution of 350 microm through the deposition of biologically active proteins. This method demonstrates a combination of off-the-shelf inkjet printing and biomaterials and has potential to be adapted to tissue engineering and colony patterning applications. Adapting this method into the three-dimensional construction of cellular structures for eventual high-throughput tissue engineering using a bottom-up approach is possible.

Animals↗
PubMed2005

Dose-dependent cell growth in response to concentration modulated patterns of FGF-2 printed on fibrin.

Immobilized patterns of unmodified fibroblast growth factor-2 (FGF-2), with varying surface concentrations, were inkjet printed onto physiologically relevant fibrin substrates. Printed patterns were characterized using iodinated FGF-2 to determine FGF-2 surface concentration and retention of FGF-2 binding in vitro. MG-63 cells were uniformly seeded onto patterned substrates. Cells were exposed to defined spatial FGF-2 surface concentrations of 1-22 pg/mm(2). Cell numbers were observed to increase in register with the printed FGF-2 patterns from an initial random uniform cell distribution across the patterned and non-patterned regions. Based on time-lapse image analysis, the primary organizational response of the cells was determined to be proliferation and not migration. Cell counts on and off the FGF-2 patterns over time demonstrated an increase in cell density up to a FGF-2 surface concentration of 14 pg/mm(2). Higher surface concentrations did not result in increased cell density. In addition, the cells on the FGF-2 patterns survived longer than the cells off patterns. Our inkjet printing approach permits the systematic study of cellular responses to defined spatial surface concentrations of immobilized growth factors.

Carbocyanines↗
PubMed2006

Viability and electrophysiology of neural cell structures generated by the inkjet printing method.

Complex cellular patterns and structures were created by automated and direct inkjet printing of primary embryonic hippocampal and cortical neurons. Immunostaining analysis and whole-cell patch-clamp recordings showed that embryonic hippocampal and cortical neurons maintained basic cellular properties and functions, including normal, healthy neuronal phenotypes and electrophysiological characteristics, after being printed through thermal inkjet nozzles. In addition, in this study a new method was developed to create 3D cellular structures: sheets of neural cells were layered on each other (layer-by-layer process) by alternate inkjet printing of NT2 cells and fibrin gels. These results and findings, taken together, show that inkjet printing is rapidly evolving into a digital fabrication method to build functional neural structures that may eventually find applications in neural tissue engineering.

Animals↗
PubMed2005

DNA and protein microarray printing on silicon nitride waveguide surfaces.

Sputtered silicon nitride optical waveguide surfaces were silanized and modified with a hetero-bifunctional crosslinker to facilitate thiol-reactive immobilization of contact-printed DNA probe oligonucleotides, streptavidin and murine anti-human interleukin-1 beta capture agents in microarray formats. X-ray photoelectron spectroscopy (XPS) was used to characterize each reaction sequence on the native silicon oxynitride surface. Thiol-terminated DNA probe oligonucleotides exhibited substantially higher surface printing immobilization and target hybridization efficiencies than non-thiolated DNA probe oligonucleotides: strong fluorescence signals from target DNA hybridization supported successful DNA oligonucleotide probe microarray fabrication and specific capture bioactivity. Analogously printed arrays of thiolated streptavidin and non-thiolated streptavidin did not exhibit noticeable differences in either surface immobilization or analyte capture assay signals. Non-thiolated anti-human interleukin-1 beta printed on modified silicon nitride surfaces reactive to thiol chemistry exhibited comparable performance for capturing human interleukin-1 beta analyte to commercial amine-reactive microarraying polymer surfaces in sandwich immunoassays, indicating substantial non-specific antibody-surface capture responsible for analyte capture signal.

Biosensing Techniques↗
PubMed2004

Post-treatment of banknote printing works wastewater ultrafiltration concentrate.

A novel process of vortex settling and stage-2 ultrafiltration (UF) with alternating feed direction was used to further concentrate the concentrate produced by a stage-1 UF employed for treatment of banknote printing works wastewater. In this post-treatment process, the final concentrate volume for incineration was reduced by 4-5 times while the permeate of the stage-2 UF could be further reused in the banknote printing operation. It was noted vortex settling facilitated settling of the printing ink and the strategy of regularly alternating feed direction in the UF resulted in a higher permeate flux compared to the corresponding flux for operation without alternating feed direction. The hydraulic retention time (HRT) of the vortex settling tank (VST) used in the pilot-scale experiment was 14 min while feed direction to the stage-2 UF was alternated once every hour. Based on the pilot-scale experimental results, a full-scale system was set up. An economic analysis showed that the novel system was a cost-effective option for post-treatment of stage-1 UF concentrate. The treatment system has been successfully implemented at several Chinese banknote printing companies.

Coloring Agents↗
PubMed2004

Biological laser printing: a novel technique for creating heterogeneous 3-dimensional cell patterns.

We have developed a laser-based printing technique, called biological laser printing (BioLP). BioLP is a non-contact, orifice-free technique that rapidly deposits fL to nL scale volumes of biological material with spatial accuracy better than 5 microm. The printer's orifice-free nature allows for transfer of a wide range of biological material onto a variety of substrates. Control of transfer is performed via a computer-aided design/computer-aided manufacturing (CAD/CAM) system which allows for deposition rates up to 100 pixels of biological material per second using the current laser systems. In this article, we present a description of the apparatus, a model of the transfer process, and a comparison to other biological printing techniques. Further, examples of current system capabilities, such as adjacent deposition of multiple cell types, large-scale cell arrays, and preliminary experiments on creating multi-layer cell constructs are presented. These cell printing experiments not only demonstrate near 100% viability, they also are the first steps toward using BioLP to create heterogeneous 3-dimensional constructs for use in tissue engineering applications.

Animals↗
PubMed2003

Interactive decision support system to predict print quality.

Customers using printers occasionally experience problems such as fuzzy images, bands, or streaks. The customer may call or otherwise contact the manufacturer, who attempts to diagnose the problem based on the customer's description of the problem. This study evaluated Bayesian inference as a tool for identifying or diagnosing 16 different types of print defects from such descriptions. The Bayesian model was trained using 1701 narrative descriptions of print defects obtained from 60 subjects with varying technical backgrounds. The Bayesian model was then implemented as an interactive decision support system, which was used by eight 'agents' to diagnose print defects reported by 16 'customers' in a simulated call centre. The 'agents' and 'customers' in the simulated call centre were all students at Purdue University. Each customer made eight telephone calls, resulting in a total of 128 telephone calls in which the customer reported defects to the agents. The results showed that the Bayesian model closely fitted the data in the training set of narratives. Overall, the model correctly predicted the actual defect category with its top prediction 70% of the time. The actual defect was in the top five predictions 94% of the time. The model in the simulated call centre performed nearly as well for the test subjects. The top prediction was correct 50% of the time, and the defect was one of the top five predictions 80% of the time. Agent accuracy in diagnosing the problem improved when using the tool. These results demonstrated that the Bayesian system learned enough from the existing narratives to accurately classify print defect categories.

Adolescent↗
PubMed1994

Mortality in the British printing industry: a historical cohort study of trade union members in Manchester.

A historical cohort study of the printing industry was established after an anecdotal report of a cluster of cases of bladder cancer in a newspaper factory in Manchester. The cohort comprised some 9500 men who were members of one or other of two trade unions (the NGA and NATSOPA) in the Manchester area between 1949 and 1963. During the follow up period (1949-83) 3482 deaths occurred among men born in 1890 or later; follow up was 97% complete. The results of the study do not support the hypothesis of an occupational risk of bladder cancer in the printing industry. The NGA have a standardised mortality ratio (SMR) of 63 (95% confidence interval (95% CI) 31-113) and NATSOPA an SMR of 113 (95% CI 67-178) based on 11 and 18 deaths from bladder cancer, respectively. Men involved in newspaper letterpress printing have a high mortality from lung cancer (SMR = 179, 95% CI 144-218) that is consistent with the findings of previous studies. Increased mortality from cancer of the buccal cavity and pharynx was found for NATSOPA workers in the newspaper industry; editorial workers had an SMR of 1053 (95% CI 128-3803) and clerical workers had an SMR of 638 (95% CI 132-1864). This is consistent with a review of published studies, which strongly suggest that workers in the printing industry have an increased risk of mortality from cancers of the buccal cavity and pharynx. Socioeconomic differences in union composition, rather than occupational factors, may account for the lower mortality in the NGA compared with NATSOPA. The NGA, a craft union, had an all causes SMR of 92 (95% CI 88-97), whereas NATSOPA covered a broader span of occupations and skill levels, and had an all causes SMR of 112 (95% CI 106-117); the NATSOPA and NGA all causes rate ratio was 1.21 (95% CI 1.13-1,29).

Adult↗
PubMed2005

Occupational exposure to volatile organic compounds and mitigation by push-pull local exhaust ventilation in printing plants.

The extensive use of multiple organic solvents in offset lithographic printing causing high emissions of volatile organic compounds (VOCs) indeed poses a serious risk to printing workers' health. In this study, indoor air quality (IAQ) assessments were carried out in seven printing plants and the main objectives were to understand the effect of VOC emissions on IAQ and develop effective mitigation measures to protect workers. The thorough gas chromatography/mass spectrometry (GC/MS) measurements showed that although a variety of VOCs were presented in the indoor air, none of them was found close to individual 8-h time-weighted average (TWA) of the occupational exposure limit (OEL). The additive effect was also found below the critical value of unity. However, short-term personal exposure to total volatile organic compounds (TVOCs) was exceedingly high when a print worker carried out blanket and ink roller cleaning procedures. Therefore, the occupational health risk was mainly due to repeated short-term exposures during intermittent VOC-emitting procedures rather than long-term exposure to background VOCs. Push-pull local exhaust ventilation (LEV) was identified as an effective mitigation measure. Computational fluid dynamics (CFD) analysis was conducted to study the push-pull LEV operation. It was found that there existed a threshold LEV air flow rate for an abrupt reduction in the worker's exposure to VOCs. The reduction was less sensitive when the LEV airflow was further increased beyond the threshold. These phenomena, consistent with experimental results reported by other investigators, were explained by detailed CFD analysis showing the competition between the general ventilation and the push-pull LEV to become the dominating driving force for the resultant local flow pattern.

Air Pollutants, Occupational↗
PubMed2005

Books, printing and medicine in the Renaissance.

The history of the medical book in the Renaissance is only just beginning: there still are enormous gaps in our knowledge. Some general points may be emphasised: doctors, surgeons and apothecaries were often literate; the culture of the doctor was founded at least as much upon the book as upon practical experience. Much is known about the history of the printing press: but in focusing on expensive, luxury books such as Vesalius' Fabrica we often leave out average products such as the many other anatomy books printed in the 16th century. The most significant feature of printing is perhaps the increase of the amount and variety of what was available and accessible to readers. Looking specifically at one type of book, the plague treatise, the amalgamation of public and private allowed by the printing press becomes apparent. Knowledge was disseminated from universities to the general public: plague texts are scattered in many different private and public libraries, and any attempt at a general survey is bound to be provisional.

Books↗
PubMed1987

Computer-based system for maintaining and printing a hospital formulary.

Use of a pharmacy department personal computer (PC) with database management and word processing (WP) systems to maintain a hospital formulary is described. Data input and formatting were accomplished with IBM-PC/XT-compatible equipment, the Nutshell (Leading Edge) database management system, and the Wordperfect (WordPerfect Corporation) WP system. Computer-generated pages were photocopied and placed in loose-leaf binders; reduced copies were made for pocket-size binders. The cost of an outside printing service was eliminated, and the loose-leaf format made it possible to replace only the pages with revisions. The large binders were prominently placed and clearly marked to encourage use by physicians; the number of copies needed was reduced because individual copies were no longer provided to each physician. Excluding first-year one-time costs, the annual formulary maintenance cost (for 250 copies) was projected to be approximately one sixth the cost incurred previously, when 1300 bound formularies were printed annually. Maintaining and printing the hospital formulary on a PC facilitated the updating of the formulary and reduced printing costs.

Computer Systems↗
PubMed2002

Early prints depicting eyeglasses.

Much of the history of eyeglasses has been gleaned from studies of paintings and prints that illustrate them. A few prints from the first century of printing include spectacles and are reproduced in this article. In addition to showing their form and method of use, these prints also illustrate their symbolic value.

Eyeglasses↗
PubMed1995

A comparison of educational interventions. Multimedia textbook, standard lecture, and printed textbook.

OBJECTIVE: To compare the instructional effectiveness and efficiency of a pediatric multimedia textbook (MMTB) with that of a standard lecture and of a printed textbook in a prospective, interinstitutional study. DESIGN: Randomized, prospective cohort. SETTINGS: An urban and a rural medical school affiliated with tertiary care hospitals. POPULATION: Third- and fourth-year medical students from June 1992 to June 1993. INTERVENTIONS/OUTCOME MEASURES: Students were randomized to one of four treatment groups: (1) computer-aided instruction with MMTBs (n = 39), (2) traditional lecture (n = 39), (3) printed textbook (n = 39), or (4) a control group (n = 62). Only the control group was pretested. Following their randomized instruction, all groups were tested via a 26-question multiple-choice test. Statistical analysis was accomplished by analysis of variance of mean post-test scores. The amount of time that students spent with each educational intervention was recorded. RESULTS: Three hundred two students were eligible for the study, 267 entered the study, and 179 completed the study. The instructional effectiveness of the MMTB was greater than that of the lecture (P < .05), and it was the same as that of the printed textbook. All instructional methods were more effective than the control group (P < .05). The instructional efficiency of the MMTB was equal to that of the lecture and of the printed textbook. The subjective response to the MMTB instruction was positive. CONCLUSION: The MMTBs constitute an educationally sound alternative instructional method and have a promising future in medical education.

Adult↗
PubMed2000

Design and fabrication of cast orthopedic implants with freeform surface textures from 3-D printed ceramic shell.

Three-dimensional printing is a solid freeform fabrication process, which creates parts directly from a computer model. The parts are built by repetitively spreading a layer of powder and selectively joining the powder in the layer by ink-jet printing of a binder material. 3D printing was applied to the fabrication of sub-millimeter surface textures with overhang and undercut geometries for use in orthopedic prostheses as bony ingrowth structures. 3D printing is used to fabricate ceramic molds of alumina powder and silica binder, and these molds are used to cast the bony ingrowth surfaces of Co-Cr (ASTM F75) alloy. Minimum positive feature sizes of the ceramic mold and, therefore, minimum negative feature sizes of castings were determined to be approximately 200 x 200 x 175 microm and were limited by the strength of ceramic needed to withstand handling. Minimum negative feature sizes in the ceramic mold and, therefore, minimum positive features in the casting were found to be approximately 350 x 350 x 175 microm and were determined by limitations on removal of powder from the ceramic and the pressure required to fill these small features with molten metal during casting. Textures were designed with 5 layers of distinct geometric definition, allowing for the design of overhung geometry with overall porosity ranging from 30-70%. Features as small as 350 x 350 x 200 microm were included in these designs and successfully cast.

Ceramics↗
PubMed2006

Covalent microcontact printing of proteins for cell patterning.

We describe a straightforward approach to the covalent immobilization of cytophilic proteins by microcontact printing, which can be used to pattern cells on substrates. Cytophilic proteins are printed in micropatterns on reactive self-assembled monolayers by using imine chemistry. An aldehyde-terminated monolayer on glass or on gold was obtained by the reaction between an amino-terminated monolayer and terephthaldialdehyde. The aldehyde monolayer was employed as a substrate for the direct microcontact printing of bioengineered, collagen-like proteins by using an oxidized poly(dimethylsiloxane) (PDMS) stamp. After immobilization of the proteins into adhesive "islands", the remaining areas were blocked with amino-poly(ethylene glycol), which forms a layer that is resistant to cell adhesion. Human malignant carcinoma (HeLa) cells were seeded and incubated onto the patterned substrate. It was found that these cells adhere to and spread selectively on the protein islands, and avoid the poly(ethylene glycol) (PEG) zones. These findings illustrate the importance of microcontact printing as a method for positioning proteins at surfaces and demonstrate the scope of controlled surface chemistry to direct cell adhesion.

Aldehydes↗