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PubMed1978

[Determination of microelements in placenta I. Commun.: spectrophotometric determination of copper content of human placentas (author's transl)].

The copper content of human placenta determined by means of the Bathocuproinemethod lies at 1,21 +/- 0,18 ppm in the fresh and 7,06 +/- 1,04 ppm in the dried material. Furthermore an improved generally applicable method for decomposition of biological materials that prevents the volatilization of trace elements is described.

Copper↗

PubMed1978

[Determination of microelements in human placenta II. Commun.: Spectrophotometric determination of zinc content in human placenta (author's transl)].

The zinc content of human placenta is determinaed by means of an improved combined dithizone-zincon-method. The results obtained are given as follows: 11.13 +/- 0.62 ppm in the fresh and 64.98 +/- 3.34 ppm in the dried material.

Female↗

PubMed1983

[Nephritogenic glycoprotein isolated from human placenta (methods of isolation from placenta].

In our experiments, we succeeded in obtaining from human placenta a substance comparable to human renal nephritogenic glycoprotein prepared from GBM (glomerular basement membrane). The procedure was quite similar to that for preparation from the kidney. At first, placental terminal villi were isolated by the successive use of three metal sieves. Then trophoblast basement membranes (TrBM) were isolated from placental terminal villi by ultrasonic disruption. Finally TrBM were made soluble by trypsin digestion, after which they were further digested by pronase. Ouchterlony gel diffusion demonstrated the existence of a common precipitin line between antiserum to human renal nephritogenic glycoprotein prepared from GBM and antiserum to the sample prepared from human placental TrBM, and human renal nephritogenic glycoprotein prepared from GBM and the sample prepared from human placental TrBM. The monosaccharide composition of the sample prepared from human placental TrBM was rich in glucose (glucose, galactose and mannoside in the ratio of 1.00 : 0.74 : 1.00), and the amino acid composition of the sample contained no collagenous components. As a result of fractionation of the sample prepared from human placental TrBM by Bio Gel P200 column chromatography, the activity of renal nephritogenic glycoprotein was found within the void fraction.

Basement Membrane↗

PubMed1978

[Determination of microelements in the human placenta. III. Commun.: atomic-absorption spectrophotometric determination of Zn, Cu, Cd, Mn, Fe, and Mg contents of human placentas (author's transl)].

In human placentas from the West Berlin area is the content on Zn, Cu, Cd, Mn, Fe, and Mg determined by means of the flameless and the flame atom absorption spectrophotometry, respectively.

Cadmium↗

PubMed1977

Water and ion metabolism in placenta. I. water and ion content of rabbit placenta in different periods of gestation.

Total tissue water of rabbit plancenta decreases by 18% between the 9th day and the end of gestation. In the same period intracellular water decreases by 40% and the extracellular compartment swells. Total tissue sodium and chloride content increase by 34% and 27% respectively from the 16th to the 33rd day while potassium decreases by 30%. The intracellular concentration of these ions does not vary greatly during gestation. Moreover the total tissue magnesium content decreases by about 23% at the end of gestation while its intracellular concentration does not show marked changes. Calcium content of rabbit placenta substantially increases during gestation and intracellular calcium shows a fivefold increase from the 16th to the 33rd day of gestation. The results are discussed in terms of relation between water and ion content and placental tissue growth and aging.

Animals↗

PubMed1957