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PubMed1988

[Effect of the tripeptide melanostatin on the electrical activity of surviving slices of the olfactory cortex in the rat brain].

The effects of melanostatin (MST 10(-5) M) on transmission in the lateral olfactory tract--superficial pyramidal neurons synapses, were investigated in slices of the rat olfactory cortex. Within 3-6 min after MST addition, the EPSP amplitudes were depressed and then increased (within 6-9 to 30 min); spikes and IPSPs appeared during the latter period. The recovery of the FPs to control values at washing was slow (40-60 min). Possible mechanisms of the MST long-term excitatory effect on neural elements of the slices, are discussed.

Animals↗

PubMed1984

Somatically recorded Ca-currents in guinea-pig hippocampal and olfactory cortex neurones are resistant to adenosine action.

Inward membrane currents were recorded in tetrodotoxin-treated neurones of hippocampus and olfactory cortex in vitro after impalement with CsCl-containing microelectrodes to suppress potassium conductances. They were blocked by the Ca2+ -channel blocker Cd2+ but were unaffected by adenosine alone (up to 1 mM), adenosine (20 microM) in the presence of the uptake blocker dipyridamole (1 microM) or the stable analogue cyclohexyladenosine (100 microM). This ineffectiveness of adenosine on Ca-currents was in contrast to its known potent suppression of synaptic transmission in these preparations, and its inhibition of Ca-dependent action potentials.

Adenosine↗

PubMedDate not supplied

[Modification of rat behavioral reactions by peptides secreted by olfactory cortex cells upon their tetanization].

We studied neurotrophic properties of neuropeptide extracts from tetanized slices of the rat olfactory cortex. Two types of the extracts were studied, EP and ED, obtained from perfusates of the tetanized slices after long-term potentiation and from the slices after long-term depression, respectively. The effects of these two extracts were tested on rats using the open field and passive avoidance tests upon intranasal administration of the extracts (ca. 1 ng total protein). EP showed a neurotropic effect combined with a moderate sedative-hypnotic effect, while EP had obvious neurotropic and nootropic properties, affecting mainly the mechanisms of memory consolidation, and had an activating effect combined with a minor, delayed sedative effect. The functional significance of the secreted endogenous neuropeptides and the mechanisms of their effects are discussed.

Administration, Intranasal↗

PubMed1985

Excitatory and inhibitory effects of dopamine on synaptic transmission in the rat olfactory cortex slice.

A study has been undertaken of the effects of dopamine on excitatory transmission at the lateral olfactory tract (LOT)-superficial pyramidal cell synapse of the rat olfactory cortex slice by measuring the effects of bath-applied dopamine on the amplitudes and latencies of the surface field potentials evoked on submaximal LOT stimulation in a total of 32 preparations. In 7 (22%) slices, dopamine had no detectable effects on transmission. In the remaining preparations, dopamine (1-250 microM) depressed transmission in a concentration-dependent manner. This action was unaffected by nadolol (10 microM), phentolamine (10 microM) and picrotoxin (25 microM) but was antagonized by chlorpromazine (10 microM) and trifluoperazine (0.2 and 0.5 microM) and mimicked by bromocriptine (0.01-5 microM) and apomorphine (0.25-25 microM). Investigation of the effects of dopamine on stimulus input-evoked potential output relationships indicated that the inhibitory effect of dopamine on transmission was mediated by a reduction in pyramidal cell excitability. In 6 slices (24% of those sensitive to dopamine) low dopamine concentrations (0.1-1 microM) facilitated transmission at the LOT-superficial pyramidal cell synapse. This excitatory effect was antagonized by nadolol and phentolamine (10 microM) and also by 100 microM 2-amino-5-phosphonovalerate (an antagonist of excitatory amino acid receptors of the N-methyl-D-aspartate type) but was unaffected by chlorpromazine (10 microM) and trifluoperazine (0.2 and 0.5 microM). By a comparison with the effects of noradrenaline on transmission, it is concluded that the excitatory effects of dopamine are mediated either indirectly by the release of noradrenaline or by a direct interaction of dopamine with adrenoceptors.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMed1978

Neuronal mechanisms of the late N-wave induced in vitro in thin sections of the olfactory cortex of rats.

Experiments were done to elucidate properties of the late N-wave which was induced in vitro in thin sections of the olfactory cortex of the rat in response to stimulation of the lateral olfactory tract. The late N-wave decreased in size at a stimulation rate of more than once every 90 sec or at temperatures higher than 27 degrees C. The late N-wave was suppressed in the presence of GABA, picrotoxin or bicuculline or in the Cl-free medium. Penicillin or pentylenetetrazol, which blocked actions of GABA on the presynaptic potential, also suppressed the late N-wave. The late N-wave first appeared at postnatal ages of 18--25 days. The late N-wave reversed in polarity when recorded from the deep layers of the sections or from the cut surface of the sections. Single cells in the deep portions of the sections discharged during the late N-wave. Cells in the superficial layers fired just before or after the late N-wave. In order to explain these observations, a neuronal model for generation of the late N-wave was presented.

Action Potentials↗

PubMed1983

Diazepam increases GABA mediated inhibition in the olfactory cortex slice.

The effect of diazepam on inhibition has been examined using an in vitro preparation of the guinea-pig olfactory cortex. Diazepam (0.03-30 mumol/l) doubled the intensity and duration of the recurrent inhibitory conductance. Diazepam had no effect on single evoked excitatory post-synaptic potential (e.p.s.p.) nor any effects on the action potential or membrane electrical constants. Diazepam (0.003-100 mumol/l) also reduced the multisynaptic e.p.s.p. generated through a recurrent pathway directed at the soma when elicited during the time-course of the inhibitory conductance. Diazepam had a comparitively small effect on the monosynaptic e.p.s.p. generated on the distal dendrite. Pentobarbitone had a similar though more intense effect over a narrow concentration range (10-200 mumol/l). The inhibitory coductance is thought to be GABA-mediated. Diazepam doubled the potency of the GABA analogue, muscimol, when applied via the bathing solution, whereas a modest 50 mumol/l pentobarbitone increased muscimol potency by about four-fold. The mild but selective effect of diazepam contrasts with the more intense and general effects of pentobarbitone and supports the idea that these drugs act through different mechanisms at the GABA receptor/channel complex.

Animals↗

PubMed1988

Effects of the anaesthetic 2,6-diisopropylphenol on synaptic transmission in the rat olfactory cortex slice.

1. The effects of the general anaesthetic 2,6-diisopropylphenol (DIP) on synaptic transmission and the actions of amino acid transmitter candidates have been investigated in rat olfactory cortex slices. 2. On electrical stimulation of the lateral olfactory tract (LOT), DIP (20 to 200 microM) increased the area of those surface field potentials which reflect gamma-aminobutyric acid (GABA)-mediated transmission in a concentration-dependent manner in 6 out of 12 slices. In a series of conditioning experiments, DIP (50 microM) also potentiated GABA-mediated pre- and post-synaptic inhibition. 3. Perfusion of slices with DIP (50 microM) potentiated the reduction in the excitability of the terminals of the LOT produced by exogenous GABA in a picrotoxin-sensitive manner. 4. DIP (50 microM) markedly potentiated the surface depolarizations evoked by GABA, muscimol and 3-aminopropanesulphonic acid. The effect on the response to 3-aminopropanesulphonic acid was observed over a concentration range of DIP of 6.25 to 50 microM and was not blocked by the benzodiazepine receptor antagonist Ro 15-1788. 5. In slices in which GABA-mediated transmission was abolished by picrotoxin (25 microM), DIP (50 microM) had no significant effect on monosynaptically-evoked excitatory transmission but depressed the areas of those field potentials which reflect di-/polysynaptic excitations in a concentration-dependent manner (from between 1.6 and 6.25 to 50 microM). 6. In a series of conditioning experiments DIP (50 microM) abolished the increase in the excitability of the pyramidal cells evoked on stimulation of deep association fibres. 7. DIP (50 microM) had no significant effect on surface depolarizations evoked by N-methyl-D-aspartate, quisqualate and kainate or by the transmitter candidates L-glutamate and L-aspartate. 8. It is concluded that, at clinically relevant concentrations, DIP potentiates GABA-mediated transmission probably by an interaction with the GABA receptor complex and inhibits di-/polysynaptic excitations, possibly by inhibiting the release of excitatory transmitters.

Anesthetics↗

PubMed1978

Association and commissural fiber systems of the olfactory cortex of the rat.

The association and commissural fiber systems arising in the olfactory cortical areas caudal to the olfactory peduncle (the piriform cortex, nucleus of the lateral olfactory tract, anterior cortical nucleus of the amygdala, periamygdaloid cortex and entorhinal cortex) have been studied utilizing horseradish peroxidase as both an anterograde and a retrograde axonal tracer. In the piriform cortex two sublaminae within layer II (IIa and IIb) layer III have been found to give rise to distinctly different projections. Retrograde cell labeling experiments indicate that the association fiber projection from layer IIb is predominatnly caudally directed, while the projection from layer III is predominantly rostrally directed. Cells in layer IIa project heavily to areas both caudal and rostral to the piriform cortex. The commissural fibers from the piriform cortex are largely restricted in their origin to layer IIb of the anterior part of the piriform cortex and in their termination on the contralteral side to the posterior part of the piriform cortex and adjacent olfactory cortical areas. A projection to the olfactory bulb has also been found to arise from cells in layers IIb and III of the ipsilateral piriform cortex, but not in layer IIa. In addition to those from the piriform cortex, association projections have also been found from other olfactory cortical areas. The nucleus of the lateral olfactory tract has a heavy bilateral projection to the medial part of the anterior piriform cortex and the lateral part of the olfactory tubercle (as well as a lighter projection to the olfactory bulb); both the anterior cortical nucleus of the amygdala and the periamygdaloid cortex project ipsilaterally to several olfactory cortical areas. The entorhinal cortex has been found to project to the medial parts of the olfactory tubercle and the olfactory peduncle. The olfactory tubercle is the only olfactory cortical area from which no association fiber systems (instrinsic or extrinsic) have been found to originate. A broad topographic organization exists in the distribution of the fibers from several of the olfactory areas. This is most obvious in the anterior part of the olfactory cortex, in which fibers from the more rostral areas (the anterior olfactory nucleus and the anterior piriform cortex) terminate in regions near the lateral olfactory tract, while those from more caudal areas (the posterior piriform cortex and the entorhinal cortex) terminate in areas further removed, both laterally and medially, from the tract. Projection to olfactory areas from the hypothalamus, thalamus, diagonal band, and biogenic amine cell groups have been briefly described.

Animals↗

PubMed2000

Activation of olfactory cortex in newborn infants after odor stimulation: a functional near-infrared spectroscopy study.

In mammals, perception of smells during the first hours of life is an essential prerequisite for adaptation of the newborn to the new extrauterine world. Functional magnetic resonance studies have shown that olfactory impression is processed in the lateral and anterior orbito-frontal gyri of the frontal lobe. Near-infrared spectroscopy (NIRS) can detect changes in oxygenated [Hb O2], and deoxygenated [Hb H] Hb during cortical activation. The aim of this study was to assess by NIRS olfactory cortex activity in newborn infants receiving olfactory stimuli. Twelve males and 11 females were studied when awake at 6 h to 8 d after birth. NIRS monitoring was carried out using two optodes placed above the left anterior orbito-frontal gyri. Each newborn was exposed for 30 s to two different smell stimuli-mother's colostrum and vanilla-and to a negative control, distilled water. Changes in Hb concentration were measured over the orbitofrontal region. During exposure to vanilla, [Hb O2] increased significantly over the left orbito-frontal area in all babies. The magnitude of the [Hb O2] increase over the illuminated region during colostrum exposure was inversely related to postnatal age. We conclude that monitoring Hb changes by NIRS can be valuable in assessing olfactory responsiveness in infants.

Aging↗

PubMed1980

Convulsants antagonise inhibition in the olfactory cortex slice.

The effects of some gamma-aminobutyric acid (GABA) antagonists and other "convulsants" have been tested on neurones of the isolated olfactory cortex slice preparation of the guinea-pig using single cell intracellular and gross extracellular recording techniques. Bicuculline, picrotoxin, strychnine, leptazol, bemegride, and also theophylline and d-tubocurarine all increased the duration and amplitude of the excitatory postsynaptic potential, thereby producing a seizure-like discharge. These drugs reduced and shortened the peak conductance increase during the inhibitory postsynaptic potential (i.p.s.p.) in normal solution and after the i.p.s.p. had been prolonged by the presence of a barbiturate. The results suggested that these drugs antagonise synaptic inhibition through a common mechanism, perhaps by reducing the effect of neurally released GABA.

Animals↗

PubMed1978

Excitability increase of neurons in olfactory cortex slices of the guinea pig after penicillin administration.

The mechanism underlying the effects of penicillin on slices of the olfactory cortex of the guinea pig was examined. In a previous report it was shown that penicillin increases the amplitude of the presynaptic action potential, the population EPSP and, more strongly, the population responses of the postsynaptic cells. Moreover, the postsynaptic population responses increased in number and suggested strong repetitive firing. These results were confirmed in the present study. Analysis of stimulus-response relationships suggested that the enhancement of the postsynaptic response was due to an increase in excitability of the postsynaptic neurons by penicillin. The amplitude changes of the presynaptic action potential and the EPSP were probably largely, if not completely, due to an increase in resistance of the bathing fluid. It was found that the changes in population responses paralleled to a large extent changes in cell discharge. In addition, penicillin was found to induce spontaneous firing of the postsynaptic cells. The changes in cell discharge were consistent with an increase in excitability of the postsynaptic cells.

Action Potentials↗

PubMed1980

Inhibitory action of adenosine and adenosine analogs on neurotransmission in the olfactory cortex slice of guinea pig - structure-activity relationships.

The postsynaptic potential (PSP) was recorded from thin slices of the olfactory cortex of the guinea pig. Application of adenosine and adenine nucleotides such as 5'-ATP, 5'-ADP and 5'-AMP in the incubation medium, depressed the amplitude of the PSP without altering the presynaptic fiber potential. The other purine and pyrimidine derivatives had no inhibitory effect. The inhibitory action of adenosine and adenine nucleotides on the PSP were manifest at concentrations of 5 microM-1 mM. Adenosine, 5'-ATP, 5'-ADP and 5'-AMP were equipotent in evoking depression of PSPs. Inhibition occurred within 10-20 sec after administration of the agents and the depressant effect disappeared rapidly after the removal of the compounds from the medium. Theophylline reversed and prevented the inhibition produced by adenosine and adenine nucleotides. To test the structure-activity relationships of these compounds, adenosine analogs and adenine nucleotide derivatives were applied to the medium. The 6-aminopurine riboside (adenosine radical) was found to be essential for inhibitory action on the PSP. Among adenosine analogs, the presence of at least one hydrogen atom in the amino group at the 6-position of the purine, and the OH group at the 2'-position of the ribose was essential for inhibitory activity.

Action Potentials↗

PubMed1990

Effects of non-opioid antitussives on epileptiform activity and NMDA responses in hippocampal and olfactory cortex slices.

Three commonly used antitussive compounds were tested for their ability to block epileptiform activity recorded extracellularly from hippocampal and olfactory cortex slices maintained in vitro. Antitussives were bath-applied to brain slices either before or after epileptiform activity was induced. Dextromethorphan (DM) prevented electrically evoked epileptiform afterdischarges and arrested spontaneous bursting induced by exposure to added NMDA or to Mg2(+)-free medium. In contrast, caramiphen (CM) and carbetapentane (CB) were effective against epileptiform activity induced by Mg2(+)-free medium, but not by NMDA. Atropine was not effective in blocking epileptiform activity at concentrations 10 times the effective concentration of CM, which has known cholinolytic activity. Our results suggest that all these antitussives exert their anticonvulsant action at the DM binding site. Neither cholinolytic activity nor antagonism of the NMDA receptor-channel complex appears to be necessary for antitussives to prevent or arrest epileptiform activity. DM appears to have a separate NMDA-antagonist property in addition to its actions at the DM site. Our neurophysiological evidence supports the hypothesis that these antitussives have anticonvulsant properties independent of any action at the NMDA receptor-channel complex.

Animals↗

PubMed1984

Inhibition of GABA uptake potentiates the conductance increase produced by GABA-mimetic compounds on single neurones in isolated olfactory cortex slices of the guinea-pig.

Membrane potential and input conductance were recorded in single neurones in slices of guinea-pig olfactory cortex in vitro. gamma-Aminobutyric acid (GABA) and GABA-mimetic compounds were applied by bath-perfusion. Potency was measured as the concentration required to double the input conductance. The potency of GABA was increased (i.e. the equi-effective concentrations were reduced) by 15.5 +/- 2.3 times (mean +/- s.e. mean) on reducing external [Na+] from 144 to 20 mmol l-1, by replacement with Mg2+. Corresponding potency changes for other agonists were + 10.8 +/- 2.5 for 3-aminopropanesulphonic acid (3-APS); 3.25 +/- 1.06 for isoguvacine and 2.43 +/- 0.69 for muscimol. Nipecotic acid (0.5 mM) produced the following increases in potency: GABA 2.68 +/- 0.02; 3-aminopropanesulphonic acid, 3.11 +/- 0.07; isoguvacine, 1.92 +/- 0.34; muscimol, 2.24 +/- 0.17. The concentration of GABA in the bathing fluid necessary to double input conductance increased with increasing depth of the recording site from the cut surface. The apparent potency fell 10 times for each 60 micron depth increment up to 150 micron. The recording depth also affected the apparent potency of muscimol and 3-APS but to a lesser extent. Reduction of external [Na+] reduced the depth-dependence of both GABA and 3-APS potency. No clear change in the duration of the recurrent inhibitory postsynaptic conductance could be detected in the presence of 0.5 mmol l-1 nipecotic acid. 6 It is suggested that agonist uptake by a Na+-dependent, nipecotic acid-sensitive mechanism severely attenuates the responses of olfactory neurones to exogenous GABA and to its analogues 3-APS, muscimol and isoguvacine, but has little immediate influence on the duration of the GABA-mediated inhibitory postsynaptic conductance.

Animals↗

PubMedDate not supplied

[Effect of hydrocortisone on synaptic transmission in surviving slices of the olfactory cortex from the rat brain].

A study was made of the effect of bath-applied hydrocortisone (10(-4) M) on excitatory transmission of the lateral olfactory tract (LOT) - superficial pyramidal cell synapse of the rat olfactory cortex slice. Population EPSP and IPSP were depressed for 6-12 min., then they were increased (60 min.) by hydrocortisone. Recovery of evoked potentials was long (40-60 min.) at washing of slices hy a normal incubation medium. Habituation of synaptic potentials during LOT repeating stimulation was deteriorated by hydrocortisone application in perfusion solution. The data obtained were considered to be adaptive functions of the nervous system to a stressor.

Animals↗

PubMedDate not supplied

The influence of picrotoxin on the methylation of phospholipids of the olfactory cortex of the brain of rats.

It was demonstrated that a decrease in the intensity of methylation of phosphatidylethanolamine in sections of rat olfactory cortex is observed 15 min following the introduction of picrotoxin (10(-5) M) into the incubation medium. The decrease in the intensity of methylation of phospholipids may be closely linked to the opening of ion channels, since it is known that maximal depolarization of the membranes is observed during this period of the effect of the preparation.

Animals↗

PubMed1990

Effects of coenzymes Q2 and Q10 on the field potential of guinea pig olfactory cortex slices maintained in hypoxia.

The effects of exogenous CoQ2 and CoQ10 on field potentials were studied while brain slice preparations of guinea pig olfactory cortex were incubated in hypoxia. The potential amplitude, which diminished in hypoxia, recovered when the slices were superfused with solutions containing CoQ2 and CoQ10. Adenosine triphosphate (ATP) levels in the slices duplicated the experimental results of the potential amplitude. The effects of these ubiquinones on potentials measured in hypoxia were suppressed when the slices were incubated with a barbiturate solution.

Action Potentials↗

PubMed1995

Patch-clamp study of neurones in rat olfactory cortex slices: properties of a slow post-stimulus afterdepolarizing current (IADP).

Whole-cell recordings were made from neurones in slices of rat olfactory cortex (10-19 days old), using potassium methylsulphate-filled pipettes. Positive commands applied from -60 mV in the presence of oxotremorine-M (10-20 microgramM, 20 of 30 cells) or trans-ACPD (10-50 microgramM, 4 of 9 cells) evoked a slow inward tail current similar to the K+ -mediated tail current (I K,ADP) recorded with sharp electrodes. I ADP was reduced by hyperpolarization, showed a 27% decrease in input conductance at its peak and was depressed by Cd(2+), 4AP or high K+ but unaffected by Cs+, Ba(2+) or TEA (5 mM). IADP was significantly larger in cells between postnatal days 13 and 15 than in younger neurones (10-12 days). These data show that stable whole-cell recordings of I ADP can be made from olfactory neurones in cortical slices, and support our hypothesis that IADP is a slowly reactivating, Ca(2+) -sensitive K+ conductance.

Animals↗