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[Different composition of the cell wall polysaccharides in Saccharomyces cerevisiae S288 and in an osmosis sensitive mutant].

Determination of the polysaccharide contents and structural studies on the mannan by acetolysis and permethylation analysis shows an altered polysaccharide biosynthesis of the osmotic-sensitive mutant VY 1160 of Saccharomyces cerevisiae S 288. The mutant contains more glucan, less mannan, and less alkali-soluble glycogen. Its mannan is characterized by more short side chains and less long side chains. Its main chain is 1 leads to 6-linked, but its side chains consist of more 1 leads to 3- than 1 leads to 2-linked mannose units.

Carbohydrate Conformation↗

Nonelectrolyte fluxes across gastric mucosa in relation to gastric stimulation. Is gastric juice secreted by osmosis or exocytosis?

The effects of histamine and thiocyanate, added to the serosal bathing solution, on unidirectional fluxes of some nonelectrolytes (thiourea, methylated thiourea derivates, mannitol), and on H+, pepsinogen and mucous secretion were investigated in frog (Rana esculenta) fundic gastric mucosa. Histamine (10(-4) M) increases significantly the outfluxes (serosa to mucosa fluxes) of only thiourea and its derivates (but not mannitol) and the stimulation is the greater the more lipidsoluble the nonelectrolyte is. Influxes (mucosa to serosa fluxes) of the same molecules are not affected. In parallel histamine stimulates H+-secretion but does not modify pepsinogen and mucous secretion. SCN- (10(-2) M) inhibits the histamine effect on thiourea outfluxes and on H+-secretion, while pepsinogen and mucous secretion are not affected. Colchicine (10(-4) M) pretreatment inhibits the histamine effect on outfluxes and H+-secretion. It is concluded that: (1) histamine induces a secretion of nonelectrolytes towards the lumen; (2) such secretion is correlated with the hormone-induced secretion of HCl and fluid; (3) this process is mediated by an exocytotic mechanism.

Animals↗

Local osmosis and isotonic transport.

Osmotically driven water flow, u (cm/s), between two solutions of identical osmolarity, c(o) (300 mM: in mammals), has a theoretical isotonic maximum given by u = j/c(o), where j (moles/cm(2)/s) is the rate of salt transport. In many experimental studies, transport was found to be indistinguishable from isotonic. The purpose of this work is to investigate the conditions for u to approach isotonic. A necessary condition is that the membrane salt/water permeability ratio, epsilon, must be small: typical physiological values are epsilon = 10(-3) to 10(-5), so epsilon is generally small but this is not sufficient to guarantee near-isotonic transport. If we consider the simplest model of two series membranes, which secrete a tear or drop of sweat (i.e., there are no externally-imposed boundary conditions on the secretion), diffusion is negligible and the predicted osmolarities are: basal = c(o), intracellular approximately (1 + epsilon)c(o), secretion approximately (1 + 2epsilon)c(o), and u approximately (1 - 2epsilon)j/c(o). Note that this model is also appropriate when the transported solution is experimentally collected. Thus, in the absence of external boundary conditions, transport is experimentally indistinguishable from isotonic. However, if external boundary conditions set salt concentrations to c(o) on both sides of the epithelium, then fluid transport depends on distributed osmotic gradients in lateral spaces. If lateral spaces are too short and wide, diffusion dominates convection, reduces osmotic gradients and fluid flow is significantly less than isotonic. Moreover, because apical and basolateral membrane water fluxes are linked by the intracellular osmolarity, water flow is maximum when the total water permeability of basolateral membranes equals that of apical membranes. In the context of the renal proximal tubule, data suggest it is transporting at near optimal conditions. Nevertheless, typical physiological values suggest the newly filtered fluid is reabsorbed at a rate u approximately 0.86 j/c(o), so a hypertonic solution is being reabsorbed. The osmolarity of the filtrate c(F) (M) will therefore diminish with distance from the site of filtration (the glomerulus) until the solution being transported is isotonic with the filtrate, u = j/c(F).With this steady-state condition, the distributed model becomes approximately equivalent to two membranes in series. The osmolarities are now: c(F) approximately (1 - 2epsilon)j/c(o), intracellular approximately (1 - epsilon)c(o), lateral spaces approximately c(o), and u approximately (1 + 2epsilon)j/c(o). The change in c(F) is predicted to occur with a length constant of about 0.3 cm. Thus, membrane transport tends to adjust transmembrane osmotic gradients toward epsilonc(o), which induces water flow that is isotonic to within order epsilon. These findings provide a plausible hypothesis on how the proximal tubule or other epithelia appear to transport an isotonic solution.

Animals↗

Osmosis: a microcomputer laboratory teacher.

A computer simulation of osmotic processes has been developed. Several experimental setups are graphically simulated and may be freely selected by the user. The simulation analyzes the basic factors which determine the osmotic pressure and volume flow of a solution (e.g. concentration, temperature, activity coefficient, Staverman's coefficient and external pressure). The program was written in Turbo Pascal to operate in IBM-PCs or compatibles. High quality graphical display of results maintains the students' interest. A user's guide and continuous help for the available options simplify its use. Computer simulation seems to be a very well suited aid for teaching osmotic phenomena, improving both theoretical knowledge and the ability of the students to empirically demonstrate some of the basic laws of osmotic phenomena.

Animals↗

Pulsing reverse osmosis as the mechanism underlying fluid exchange.

Problems inherent in the existing theories of fluid exchange are discussed. An alternative theory based on the interaction between the pulsing of the capillary pressure and the osmotic gradient between plasma and interstitial fluid is offered. Theoretic values for plasma and interstitial osmolalities are calculated. These are similar to the pressure found in the Bowman's capsule in the kidney. The theory offers a possible explanation for the pulsatile nature of the blood pressure, the formation of oedema and the stasis of fluid exchange in shock.

Animals↗

Biofiltration pretreatment for reverse osmosis (RO) membrane in a water reclamation system.

Biofouling control is considered as a major challenge in operating membrane systems. A lab-scale RO system was setup at a local water reclamation plant to study the feasibility of using biofiltration as a pretreatment process to control the biofouling. The biological activity in the RO system (feed, product, reject streams) was tested using the standard serial dilution plating technique. Operational parameters such as differential pressure (DP) and permeate flowrate of the system were also monitored. Effects of biofilter on AOC and DOC removals were investigated. Biofiltration was found to be a viable way of assimilable organic carbon (AOC) and dissolved organic carbon (DOC) removals, with removal efficiencies of 40-49% and 35-45% at an empty bed contact time (EBCT) of 30 min. It was also found that using the biofiltration as a pretreatment reduced the rate of biofouling. It took only about 72 h for biofouling to have a significant impact on the performance of the RO membrane, when the system was operated without using biofiltration as pretreatment. There was, however, a five times increase in operational length to more than 300 h when biofiltration was used. This study presented the suitability of the biofilter as an economical and simple way of biofouling control for RO membrane.

Biofilms↗

Electro-oxidative abatement of low-salinity reverse osmosis membrane concentrates.

The present study encompasses the application of electrolysis as novel treatment technique for the abatement of low-salinity concentrates generated from the filtrative treatment of water and wastewater. Four different materials have been tested as anode for a number of brine samples in a one-compartment electrolytic cell in galvanostatic mode. It was found that PbO(2) and SnO(2) anodes initiated electrochemical precipitation through an increase of the pH. Boron-doped diamond (BDD) and RuO(2) anodes successfully oxidised the pollutants in the brine and a linear removal of total ammonia nitrogen (TAN) and chemical oxygen demand (COD) was observed during the first phase of oxidation. Oxidation was predominantly achieved through indirect hypochlorite bulk oxidation; the higher oxidation rate and extent for the BDD anode was attributed to the higher selectivity and activity of the latter. Overall performance of the BDD electrode was higher than for RuO(2): higher rates for TAN (17.9 vs. 13.5mg/Ah) and COD (74.5 vs. 20.0mg/Ah) removal as well as higher overall current efficiencies (35.2% vs. 14.5%). Extensive colour removal was observed for both anodes (>90% decrease in absorbency at 455 nm).

Electrolysis↗

Removal of biological and non-biological viral surrogates by spiral-wound reverse osmosis membrane elements with intact and compromised integrity.

The removal of bacteriophage MS2 and fluorescent-dyed polystyrene microspheres with intact and purposely compromised spiral-wound RO membrane elements was investigated. MS2 rejection with intact membrane elements was >99.9995%. A model developed for data evaluation revealed that the advective passage of MS2 through imperfections of intact membrane elements was <2 x 10(-5)% of the overall product water flow produced. The advective passage of MS2 and microspheres through a pinhole induced in one of the elements was 0.05-0.1% of the overall product water flow. Prolonged testing of both intact and compromised elements resulted in increased MS2 rejection corresponding to advective MS2 passage through membrane imperfections of <3 x 10(-7)% of the overall product water flow. The permeate flow rate obtained with an element with a larger pinhole was 5-13% greater than that of the intact element, and the corresponding rejection of MS2 and microspheres was similar to that observed for sodium chloride. The use of a cracked o-ring in the connection of the permeate tube to the element vessel end-cup resulted in advective passage of MS2 through the crack of <0.0001% of the overall permeate flow.

Fluorescent Dyes↗