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PubMed1995

The Nebraska "Network of Drug-Free Youth" program.

This paper describes Nebraska's six-year experience with a successful prevention program to form and maintain local drug-free youth groups. More than 4,500 youth were involved at the time of a six-year evaluation survey. Nearly 91% of group members who responded reported being alcohol-free in the previous 30 days and almost all (97%) reported being tobacco-free. Thirty-eight percent of members met one or more of the federal criteria for "high-risk" youth. Sixty-eight percent of responding high-risk youth who previously used alcohol credited group membership with helping them quit or reduce use. The program appears to assist in delaying onset of alcohol, tobacco, and other drug use for youth in the program, and to assist in decreasing or eliminating use of alcohol and tobacco by adolescents who used before participating.

Adolescent↗
PubMed2002

Rural Nebraska elementary school educators teach nutrition concepts.

The purpose of this study was to determine if differences exist in the teaching of nutrition to students in grades one to four in rural (less than 10,000 population); midsized (10,000 to 99,999); and urban (100,000 or more) counties in Nebraska. Surveys were sent to one-fifth of educators teaching grades one to four (n = 1,232); the response rate was 37.7%. Sixty-eight percent of the teachers responded that the teaching of nutrition was of very high or high priority in the elementary curriculum. Nutrition was taught as part of a nutrition/health unit as well as being integrated into other subject areas. The resources the teachers used were not diffrent by county population size. Significant differences (p < .05) were observed among county groups as to the frequency of teaching the recommended intakes of grain products, vegetables, fruits, dairy, and meats, with urban teachers teaching these concepts least often. The majority of the teachers rarely, if ever, taught serving sizes and which food groups are in combination foods, with no differences among county groups. The formal training that the respondents had in nutrition was not diffrent among groups. A larger percentage of teachers in rural and midsized community groups taught food selection concepts more consistently or frequently than did their urban counterparts.

Chi-Square Distribution↗
PubMed2006

The characteristics of successful family physicians in rural Nebraska: a qualitative study of physician interviews.

CONTEXT: In studying physician shortage issues, few studies have utilized individual interviews, allowing rural physicians to voice their own stories. PURPOSE: To explore rural physicians' unique characteristics affecting their decisions and satisfactions with practice in a rural area. METHODS: A qualitative interview study with 11 Nebraska family physicians practicing in rural (frontier) areas. FINDINGS: Previous experience and preference for a rural lifestyle, desire for autonomy, a wide scope of practice, and close relationships with patients defined those physicians choosing rural practice.

Female↗
PubMed1978

Nitrogen fixation (acetylene reduction) associated with roots of winter wheat and sorghum in Nebraska.

Root segments and root-soil cores (6.5-cm diameter) from fields and nurseries of winter wheat and sorghum were tested for N2 fixation by using the acetylene reduction assay. Wheat samples (approximately 1,200) from 109 sites generally had low or no activity (0 to 3.1 nmol of C2H4 produced per h per g [dry weight] of root segments), even after 24 h of incubation. However, a commercial field of Scout 66, located in western Nebraska, exhibited appreciable activity (290 nmol of C2H4 produced per h per g [dry weight] of root segments). Of 400 sorghum lines and crosses, grain sorghums (i.e., CK-60A, Wheatland A, B517, and NP-16) generally exhibited higher nitrogenase activity than forage sorghums or winter wheats. CK-60A, a male sterile grain sorghum, was sampled at four locations and had the most consistent activity of 24 to 1,100 nmol of C2H4 produced per h per core. The maximum rate extrapolated to 2.5 g of N per hectare per day. Numerous N2-fixing bacterial isolates were obtained from wheat and sorghum roots that exhibited high nitrogenase activity. Most isolates were members of the Enterobacteriacae, i.e., Klebsiella pneumoniae, Enterobacter cloacae, and Erwinia herbicola.

Acetylene↗
PubMed2006

Performance of a commercial immunoglobulin M antibody capture assay using analyte-specific reagents to screen for interfering factors during a West Nile virus epidemic season in Nebraska.

In 2003, the Nebraska Public Health Laboratory tested more than 10,371 serum and 516 cerebral spinal fluid specimens. Results showed that without performing the interfering factors screen for specimens in the low positive index value range of >1.1 to <or=3.5, a false positivity rate of 6.5% would have been realized.

Antibodies, Viral↗
PubMed2026

New purple sulfur bacteria genomes from Nebraska salt marsh enrichments.

Two genomes from a Winogradsky column enrichment from the Nebraska salt marshes were sequenced. The analysis of whole-genome phylogeny and average nucleotide identity comparisons indicated that these belong to species of purple sulfur bacteria, Halochromatium and Ectothiorhodospira marina, that have not been described before.

Ectothiorhodospira↗
PubMed1978

Physicochemical properties of Nebraska calf diarrhea virus hemagglutinin.

Highly purified Nebraska calf diarrhea virus (NCDV) was prepared by cesium chloride density gradient centrifugation. The effect of temperature, pH, different concentrations of formaldehyde, chloroform, ether, ethyl alcohol, and methyl alcohol on NCDV hemagglutinin and virus morphology was studied. NCDV hemagglutinin was inactivated by temperature, pH 2.0, chloroform, ethyl alcohol, and methyl alcohol.

Alcohols↗
PubMed1991

State-university collaboration in Nebraska: public psychiatry residency training in a rural area.

The shortage of well-trained psychiatrists in the public sector is particularly severe in rural states, where state hospitals are often isolated from the professional resources available in cities. The combined department of psychiatry at the University of Nebraska Medical Center and Creighton University in Omaha has developed a residency training program at a rural state hospital 150 miles away. To bridge the distance, residents attend classes in Omaha once a week and faculty supervisors fly to the hospital once a week. Residents at the remote facility are subject to the same evaluation procedures used for residents at other facilities. The author describes the role of charismatic individuals in initiating the state-university collaboration, addresses the need for a structure to support the relationship in the absence of its founders, and discusses accreditation of residency training programs based in facilities that are remote from the medical school campus.

Academic Medical Centers↗
PubMedDate not supplied

Copper in drinking water, Nebraska, 1994.

In 1993, the levels of copper (Cu) in much of Nebraska's drinking water exceeded the U.S. Environmental Protection Agency's (EPA) action level of 1.3 mg/L. To determine the association of copper with gastrointestinal (GI) illness in August 1994 the authors interviewed persons living in households with 1993 Cu levels > 3 mg/L (51 households), 2-3 mg/L (54 households), and < 1.3 mg/L (43 households). Cases were defined as persons who had experienced the rapid onset of vomiting or nausea with abdominal pain during the preceding two weeks. To validate their exposure index, the authors immediately resampled drinking water in the households of 25 cases and 27 controls matched for age. Reassessment in December 1994 of the exposure and case status of the original cohort showed no association between copper in drinking water in 1993 and case status in 1994. The risk of GI illness seemed no greater with Cu > 3 mg/L (RR = 1.03; 95% CI = 0.43, 2.49) or 2-3 mg/L (RR = 0.50; 95% CI = 0.18, 1.41) than with Cu < 1.3 mg/L. The 1993 Cu concentrations could not be reproduced in August 1994; resampling in December 1994 again showed no association between GI illness and Cu exposure (RR > 1.3-2.9 mg/L, < or = 1.3 mg/L = 0.25, 95% CI = 0.10, 0.64, and RR > or = 3.0 mg/L, < or = 1.3 mg/L = 0.36, 95% CI = 0.09, 1.49).

Case-Control Studies↗
PubMed1992

Autosomal dominant spinocerebellar ataxia: locus heterogeneity in a Nebraska kindred.

SCA1 is an adult-onset autosomal dominant ataxia that is genetically linked to loci on chromosome 6p. A highly informative GT-repeat marker, D6S89, has been closely linked to the SCA1 locus in five large kindreds. We have used this marker to perform linkage analysis in a smaller autosomal dominant ataxia family consisting of five generations designated as the Nebraska kindred. This kindred includes 33 affected (12 living) and 40 first-generation at-risk individuals. We examined eight affected individuals; all had gait and limb ataxia. We analyzed the D6S89 locus by the polymerase chain reaction. Based on the analysis of 31 individuals from this kindred, we statistically excluded linkage to D6S89 for moderate-to-tight linkage (less than 11% recombination). These data clearly demonstrate genetic heterogeneity among the autosomal dominant ataxias. In addition, we obtained linkage data for HLA-A and SCA1 in this kindred. Comparison of HLA-A with D6S89 shows the latter marker to be more powerful. Use of D6S89 and other highly polymorphic markers in this region will greatly facilitate genetic classification of ataxias and make presymptomatic diagnosis of SCA1 feasible.

Adolescent↗
PubMed2004

Androgenic and estrogenic activity in water bodies receiving cattle feedlot effluent in Eastern Nebraska, USA.

Studies reveal that surface waters worldwide are contaminated with hormonally active agents, many released from sewage treatment plants. Another potential source of aquatic hormonal contamination is livestock feedlot effluent. In this study, we assessed whether feedlot effluent contaminates watercourses by measuring a) total androgenic [methyltrienolone (R1881) equivalents] and estrogenic (17beta-estradiol equivalents) activity using the A-SCREEN and E-SCREEN bioassays and b) concentrations of anabolic agents via gas chromatography-mass spectroscopy and enzyme-based immunoassays. Water samples were collected over 3 years from up to six sites [all confluent with the Elkhorn River, Nebraska, USA: a feedlot retention pond (site 1), a site downstream from site 1 (site 2), a stream with intermediate livestock impact (site 3), and three sites with no observable livestock impact (sites 4-6)] and two sources of tap water. In 1999, samples from site 1 contained 9.6 pM R1881 equivalents and 1.7 pM 17beta-estradiol equivalents. Site 2 samples had estrogen levels similar to those in site 1 samples but lower androgen levels (3.8 pM R1881 equivalents). Androgen levels in site 3 samples were similar to those in site 2 samples, whereas estrogen levels decreased to 0.7 pM 17beta-estradiol equivalents. At site 6, androgen levels were approximately half those found at site 3, and estrogen levels were comparable with those at site 3. Sampling in later years was limited to fewer sites because of drought and lack of permission to access one site. Instrumental analysis revealed estrone but no significant levels of resorcylic acid lactones or trenbolone metabolites. Tap water was devoid of hormonal activity. We conclude that feedlot effluents contain sufficient levels of hormonally active agents to warrant further investigation of possible effects on aquatic ecosystem health.

Androgens↗
PubMed2006

A partnership between the University of Nebraska College of Medicine and the community.

Over the last 20 years, older people have served as teachers for students as part of the formal curriculum in geriatrics at the University of Nebraska Medical Center. In recent years, we have supported a more in-depth, longitudinal experience that connects medical students with elders in the community. The program was initiated as a special project of our Aging Interest Group and is called the Senior Companionship Program (SCP). Although the SCP is optional, when combined with the required experiences for medical students, it triples the contact time that students have with older adults in community settings. The student-run SCP is fostering positive attitudes towards older adults. Comments from participating students indicate that the SCP is a valuable experience, and well worth their time commitment. We describe the program structure, changes to the program based upon the input of participants, as well as the program's strengths, limitations, and prospects.

Adult↗
PubMed1993

Health care for Nebraska's medically indigent.

In this study, several alternatives for the provision of health care to the medically indigent of Nebraska were analyzed quantitatively and qualitatively. These alternatives were: expansion of county medical assistance programs, state-purchased health insurance policies, Medicaid expansion, revenue pool to redistribute charity-care losses, all-payer rate system, mandated employer-purchased health insurance, and charity-care districts. Under four future scenarios, alternatives were ranked on the basis of program costs, a sensitivity analysis, and qualitative criteria. This analysis found that state-purchased health policies would result in lower program costs than either an increase in county assistance programs or employer-mandated health insurance. Medicaid expansion would reach fewer than one-third of the state's medically indigent. A revenue pool and all-payer rate system are the least costly alternatives but depend on the continuing good will of providers.

Budgets↗
PubMed2003

Resistance status of house flies (Diptera: Muscidae) from southeastern Nebraska beef cattle feedlots to selected insecticides.

The status of resistance to three insecticides (permethrin, stirofos, and methoxychlor), relative to a laboratory-susceptible colony, was evaluated in field populations of house flies, Musca domestica L., collected from two beef cattle feedlots in southeastern Nebraska. Topical application and residual exposure to treated glass surfaces were suitable methods for determining the resistance status of house flies to permethrin, stirofos, or methoxychlor. However, in most cases, residual exposure was more sensitive in resistance detection (i.e., higher resistance ratios). The field populations tested were moderately resistant to permethrin (RR = 4.9-fold and RR = 7.3-fold, for topical application and residual exposure, respectively) and extremely resistant to stirofos and methoxychlor (not accurately quantifiable because of low mortality at the highest possible concentrations or doses). Probable explanations for the resistance status of these house fly populations and implications for global feedlot fly management are discussed.

Animals↗
PubMed2000

Stylocephalus occidentalis n. sp. (Apicomplexa: Eugregarinida: Stylocephalidae) from Trimytis pruinosa (Coleoptera: Tenebrionidae) in the Nebraska Sandhills.

Stylocephalus occidentalis n. sp. (Apicomplexa: Eugregarinida) is described from Trimytis pruinosa (Coleoptera: Tenebrionidae) collected from Keith County in the Sandhills of western Nebraska. Measurements are means in micrometers. Developing trophozoites solitary; epimerite a complex of terminal epimerite and intercalating diamerite; epimerite shallowly ovoid to transversely elliptoid, with transverse basal constriction at junction with diamerite, length 0.5-1 times width, approximately 3-4 times that of diamerite; width approximately equal to that of diamerite; diamerite roughly cylindrical to spindle-shaped, without significant anterior taper, little or no evidence of longitudinal folds, length approximately twice width. Association late, frontal, isogamontic. Gamont protomerite depressed ovoid to very broadly ovoid, length 27.3, width 35.1, anterior distance to widest point 15.4. Protomerite-deutomerite septum clearly marked and constricted, width 34.6. Deutomerite often with distinct marginal crenulation, narrowly obovoid to very narrowly obovoid, length 356.5, maximum width 57.6, anterior distance to widest point 26.3, equatorial width 35.1, +/-12.5, 29. Total length 381.5. Nucleus ellipsoid, length 32.5, width 18.8; with 0 or 2 polysomal endosomes. Gametocysts roughly spherical; diameter 205.0; wall desiccating to become paper-like, slightly papillated, dehiscing by simple rupture, releasing oocysts in coiled chains, epispore packet absent, gametocyst residuum present. Oocysts dark brown to black, axially asymmetric, broadly deltoid, gibbous in lateral aspect, slightly keeled in dorsal aspect; length 9.8, height 7.9; with slight terminal protuberances and 2 central, spherical residua.

Animals↗
PubMed2000

Sarcocystis campestris from naturally infected 13-lined ground squirrels, Spermophilus tridecemlineatus tridecemlineatus, from Nebraska.

Grossly visible sarcocysts were seen in the skeletal muscles of 1 of 12 13-lined ground squirrels, Spermophilus tridecemlineatus tridecemlineatus, collected in Nebraska. The tissue cyst wall was up to 5.0 microm thick and contained spikelike projections. Transmission electron microscopy of tissue cysts revealed they were similar to Sarcocystis campestris Cawthorn, Wobeser, and Gajadhar, 1983, previously known only from experimental infections in Richardson's ground squirrel Spermophilus richardsonii. Prominent electron-dense bodies were observed lining the microfilaments present in the spikelike projections of the sarcocyst wall. This is the first report of S. campestris in a natural intermediate host and the first report of this parasite outside of Saskatoon, Canada.

Animals↗
PubMed2002

Clitellocephalus americanus n. gen., n. sp. (Apicomplexa: Eugregarinida: Gregarinidae) from Cratacanthus dubius (Coleoptera: Carabidae: Harpalinae) in the Nebraska sandhills and Clitellocephalus ophoni n. comb. (Apicomplexa: Eugregarinida: Gregarinidae) from Ophonus pubescens (Coleoptera: Carabidae: Harpalinae) in Sète, France.

Clitellocephalus americanus n. gen., n. sp. (Apicomplexa: Eugregarinida: Gregarinidae) is described from Cratacanthus dubius (Coleoptera: Carabidae) collected from Keith County in the Sandhills of western Nebraska. Clitellocephalus ophoni n. comb. is redescribed using original type material from Ophonus pubescens (Coleoptera: Carabidae) collected in Sète, France. Clitellocephalus n. gen. is distinguished by a deltoid epimerite with an internal anterior obconoid structure and a basal toroidal vacuole, which is retained in gamonts. Protomerites are broadly elliptical to cylindrical; deutomerites are narrowly obovate. Association is precocious, caudofrontal, and biassociative, with the satellite protomerite engulfing the posterior end of the primite deutomerite to form an interlock. Gametocysts are spherical. Sporoducts are present but reduced and irregular in number. Oocysts are dolioform, dehiscing in chains. The species described herein are differentiated by their overall size and relative proportion of cellular structures.

Animals↗