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The morphology of the diencephalon in the Prosimii. II. The Lemuroidea and Lorisoidea. Part II. Epithalamus, subthalamus and hypothalamus.

This study (Part II) deals with the comparative structure of the epithalamus, subthalamus and hypothalamus. The same prosimian species used in the study of the thalamus and metathalamus (Part I) are used here. The epithalamus does not show any remarkable change in the phylogeny of the prosimian diencephalon. In the subthalamus, the nucleus subthalamicus enlarges progressively in size, and shows a very close relationship to the zona incerta, the fields of Forel and the pregeniculate body. The zona incerta is observed to consist of two parts the ventral part relating to the nucleus subthalamicus and the dorsal part ot the nucleus reticularis. The fields of Forel is well differentiated into fields H1 and H2. The hypothalamus is described here having four regions--preoptic, suraoptic, infundibular and mamillary. The preoptic and supraoptic regions appear to be indivisible in all prosimian species, for the constituent nuclei of both regions extend rostrally and caudally in the anterior part of the hypothalamus. The nucleus supraopticus is evidently complicated in structure, as it appears not only to have two separate parts, but also an isthmus that connects these parts. This interlinking strand of cells is termed not the nucleus supraopticus diffuses but the nucleus/area commissuralis postopticus. The nucleus paraventricularis is a massive nucleus, and appears to have a non-neurosecretory part and a neurosecretory part. An interesting feature has been observed in certain prisimian species such as Galago demidovii, Lepilemur and Loris gracilis, the nucleus paraventricularis accessorius which may be regarded as an erratic derivative of either nucleus paraventricularis or nucleus supraopticus. The nucleus tuberalis lateralis makes its first definitive appearance in the Prosimii. The nucleus ventromedialis hypothalami appears to be larger and more clearly defined than the nucleus dorsomedialis hypothalami. From the latter nucleus, a cellular condensation has been observed to stretch lateralwards--nucleus dorsolateralis hypothalami. The hypothalamic areas--anterior, dorsal, lateral and posterior--are not very much different among the prosimian species, although the anterior and posterior hypothalamic areas appear to have more attributes of a nucleus than an area. The mamillary region is very well developed in all prosimian species. The medial mamillary nucleus is clearly divided into several parts. The nucleus mamillaris lateralis is poorly developed, but can be identified lying between the lateral part of the medial mamillary nucleus and the nucleus intercalatus. In regard to the latter nucleus, there is some topographical confusion, but the nucleus intercalatus is easily identified as the bed nucleus of the supramamillary commissure.

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The binding of staphylococcal protein A by the sera of different animal species.

The capacity of purified immunoglobulin or serum to bind (125I)-labeled staphylococcal protein A (SPA) was measured by means of an immunofiltration assay that facilitated the examination of large numbers of sera and required only a minute quantity of each. Sera from 80 species, including humans, laboratory animals, domestic animals, and a variety of African mammals were examined. A wide interspecies variation in the SPA-binding capacity of serum immunoglobulins was confirmed. Only small variations were observed among individuals within the same species with one notable exception. A greater than 10,000-fold variation in SPA-binding capacity was observed among sera from nine goats. Interspecies differences in serum SPA-binding capacity correlated well with taxonomic differences, and the serum SPA-binding capacity correlated well with taxonomic differences, and the serum SPA-binding capacities of African mammals corresponded closely to those of their more common relatives. The sensitivity of antibody detection by indirect (125I)SPA immunoassay was shown to be determined mostly by the SPA-binding capacity of the serum examined. The titer of antibody to influenza A/Texas/1/77(H3N2) virus in sera from several different species, when measured by (125I)SPA immunofiltration assay, was directly proportional to the SPA-binding capacity of the serum and was not proportional to the hemagglutination inhibition titer. An (125I)SPA immunofiltration assay for antibody to Lassa virus in the serum of Mastomys natalensis (which binds SPA only one-thousandth as well as human serum) was at least as sensitive as the standard fluorescent antibody assay.

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Further observations on the occurrence of taste buds in the papilla palatina of primates.

1. Taste buds occur in the epithelium of the papilla of Galago crassicaudatus and demidovi, Perodicticus potto and Microcebus murinus. The taste buds are found single or in groups of 2 or 3. A taste area ocurs in Galago crassicaudatus and Perodicticus potto. 2. The pattern of the distribution of the taste buds in the species studied is basically the same as found previously in Nycticebus and Tupaia. The taste buds are found in the oral part of the papilla palatina exclusively, far oral to the orifices of the ductus nasopalatinus and vomeronasalis. 3. From the orientation of the taste buds it can be concluded that they taste substances entering the sulcus papillae palatinae, because the buds point at the entrances and at the lumen of that sulcus. Since substances may approach the anterior part of the papilla from the oral direction the taste buds in this area point rostrally. In Galago the anteriormost taste buds are situated in the median plane pointing at the slit between the epithelial laminae of the labial folds. 4. Many taste buds were found in the process of disintegration.

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The morphology of the diencephalon in the Prosimii. II. The Lemuroidea and Lorisoidea. Part I. Thalamus and metathalamus.

This study (Part I) covers the comparative structure of the thalamus and metathalamus in the prosimian superfamilies, Lorisoidea and Lemuroidea. Several lemuroid specimens, namely Lemus, Cheirogaleus, Microcebus, Avahi and Daubentonia, and lorisoid specimens such as Galago, Perodicticus and Loris have been used in this extensive study. The anterior nuclear group does not show much difference among these species, although attention is focussed on the differentiation of the principal anterior nucleus into anteromedial and anteroventral parts; the latter increases in structural complexity. The anterodorsal nucleus has regressed into a small, flattened cap-line structure covering the dorsal surface of the anteroventral nucleus. The midline thalamic nuclei are more or less the same throughout the entire prosimian primate scale, though the gradual fusion of the nucleus rhomboidalis with the nucleus centralis medialis is observed from Microcebus to Daubentonia. The medial thalamic nuclear region is dominated by the nucleus mediodorsalis which is clearly differentiated into dorsomedial magnocellular and ventrolateral parvocellular protions. A third division consisting of rather large, darkly staining cells have been observed in the caudal region of the nucleus mediodorsalis. The nuclei centrum medianum and parafascicularis are both large in size, but is not completely demarcated from each other. The dorsolateral thalamic nuclei--the nuclei laterales dorsalis, intermedius and posterior, are well developed, but cannot be distinguished from one another. The pulvinar shows a remarkable phylogenetic development; it does not only enlarge, but is differentiated into several distinct parts. The ventrolateral thalamic nuclear group is comparably larger in structural size than the dorsolateral thalamic nuclei. The nuclei ventrales anterior and lateralis are not clearly demarcated from each other, but on both cyto- and myelo-architectonic grounds, are separate entities. The nucleus ventralis posterior is differentiated into lateral, medial and inferior parts. The posterior thalamic nuclei show the beginnings of regression. The nucleus pretectalis remains comparatively large in size and divided into medial and lateral parts. The nucleus posterior thalamicus is either absent or involuted into other nuclei. The nucleus limitans makes its first appearance in the Prosimii. The lateral geniculate body is very variable in size in all respects among the prosimian species, that is, in the dorsoventral shift along the lateral surface of the thalamus, the cellular differentiation of the lateral geniculate nucleus into 5 or 6 layers, the topographical position of the pregeniculate nucleus and the degree of inversion. The medial geniculate body is shown to be clearly subdivided on cyto-architectonic grounds into dorsomedial and ventrolateral parts; a magnocellular protion can be seen on the dorsal region of this nucleus, and appears to be closely related to the nucleus suprageniculatus.

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Cytomegalovirus in the parotid gland of a slow loris, Nyctecibus coucang.

Sporadic enlarged cells with both nuclear and cytoplasmic viruses were found in the intralobular ducts of the parotid salivary gland of an adult female slow loris; these ducts are homologous to striated ducts in the salivary glands of other primates. The duct cell nuclei contained reticular inclusions and virions in all stages of development. Cytoplasmic virions were, in almost every case, confined to vacuoles; only a very few were free in the cytosol. The viruses conformed in ultrastructure to that of cytomegaloviruses described in other species. This may be the first observation by electron microscopy of in situ cytomegaloviruses in the salivary glands of a nonhuman primate.

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