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Results for “Karyotype analysis”

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PubMed1977

Karyotype analysis of teratocarcinomas and embryoid bodies of C3H mice.

Karotype and capacity for differentiation were determined in four transplantable teratomas, and their embryoid bodies, derived from C3H mouse embryos. An apparently normal karyotype was retained by one tumour and one subline that were able to differentiate into a wide range of tissues, but some chromosomal alterations were found in the two tumours and one subline that showed almost identical restrictions in their capacity for differentiation. Trisomy for chromosome 11 was shared by all three restricted tumours; two of the tumours had similar length changes in the same two chromosome (1 and 14) while the third was generally trisomic for four other chromosomes.

Animals↗
PubMed1994

Karyotypic analysis of seven established human esophageal carcinoma cell lines.

Fifty metaphases from each of seven esophageal carcinoma cell lines (TE-1, TE-4, TE-5, TE-6, TE-9, HCE-6, and HCE-8) were karyotypically analyzed for both numerical and structural chromosomal abnormalities. The modes of numerical variation found in the TE cell lines were as follows: 1) a wide range of variation in chromosome numbers (45-100)/cell with a modal number of 52 (TE-1); and 2) a narrow range of variation at the hypertetraploidy level (TE-4), hypotriploidy level (TE-5 and TE-6) and hypertriploidy level (TE-9). The distribution of chromosomal number/cell in cell lines HCE-6 and HCE-8 was extremely diverse. It was difficult to find a modal number in the range of 94-117 in the HCE-6 line or in the range of 45-52 in the HCE-8 line. The structural changes were extensive in all of these cell lines. Chromosomes most frequently involved in the structural changes were 1, 2, 3, 6, 7 and 9. Most of these abnormalities resulted from simple deletion, duplication, insertion, inversion or translocation. A few of them were complicated rearrangements. No breakpoint was shared by all of the cell lines.

Chromosome Aberrations↗
PubMed1986

Karyotypic analysis of diploid or near diploid metastatic Harvey ras transformed rat embryo fibroblasts.

The hypothesis of tumor progression proposed by Nowell [P. C. Nowell, Science (Wash. DC), 194: 23-28, 1976] states that one mechanism for the development of the metastatic phenotype could be the induction of chromosomal instability. We have developed a new experimental system for studying the induction of the metastatic phenotype using early passage fibroblasts which become metastatic in nude mice after transformation with the Harvey ras oncogene [R. J. Muschel et al., Am. J. Pathol., 121: 1-8, 1985; R. Pozzatti et al., Science (Wash. DC), 232: 223-227, 1986]. Since the early passage fibroblasts themselves are diploid, we reasoned that this might be a system in which karyotypic change after tumor formation or metastasis might easily be evaluated. Thus, we performed cytogenetic analysis on multiple metastases and tumors which had been derived from cells transformed with the cellular Harvey ras1 oncogene and compared their karyotypes. The karyotypes of the cells isolated from 5 tumors and 14 metastases were, as far as we could determine, identical to those of the injected cells. This could easily be evaluated because of the two clones studied one was diploid; the other has a trisomy of chromosome 4 without any other detectable abnormality. These results suggest that in this system using nude mice, selection for a necessary or even advantageous chromosomal aberration does not occur during tumor formation or metastasis. Furthermore, they indicate that the presence of the ras gene itself does not induce chromosomal rearrangements or aneuploidy and that a cell can be both tumorigenic and metastatic yet remain diploid.

Animals↗
PubMed2000

Karyotypic analysis of intersexuality in Chinese from Taiyuan.

AIM: To analyze the kayrotypic patterns of 33 cases of intersexuality in Chinese from Taiyuan, China in order to further clarify its mechanism of development and the interrelationship between karyotype and phenotypic sex. METHODS: High-resolution GTG-banding chromosome technique was used to analyze the karyotype patterns. RESULTS: In these patients, 57.58% were male pseudohermaphrodites (46,XY), 18.18%, female pseudohermaphrodites (46, XX), 12.12%, true hemaphrodites, and 12.12%, other karyotypes. Although testes can be seen in 88.8% of karyotypes with Y chromosome, 73.68% of the patients were of female social sex. In 42.82% of patients the social sex is in conformity with their karyotypes. There were 2 cases of male pseudohermaphrodites, where the sex chromosome was normal, but abnormalities were found in chromosomes 9, 13, or 14. CONCLUSION: Sex chromosomes determine the direction of gonadal and sex differentiation, while the development of the normal gonad and external genitalia should have the participation of many autosomal chromosomes as well.

Adolescent↗
PubMed1995

Karyotype analysis of anuran trypanosomes by pulsed-field gradient gel electrophoresis.

The chromosomes of 12 species and isolates of anuran trypanosomes were investigated by pulsed-field gradient gel electrophoresis. Twelve to 16 chromosomes ranging from 0.45 to 2.2 megabase pairs were found in each of these trypanosomes. Minichromosomes were not observed in any of the examined isolates. Results indicate that different species of anuran trypanosomes display distinct karyotype patterns, and that isolates from the same region are similar. Our findings also reveal that most chromosome profiles of these trypanosomes are in accordance with isoenzyme and random amplified polymorphic DNA analysis data.

Animals↗
PubMed2003

Karyotype analysis in Hyacinthella dalmatica (Hyacinthaceae) reveals vertebrate-type telomere repeats at the chromosome ends.

Chromosome analysis of three different populations of Hyacinthella dalmatica (Lallem.) Trinajstić, an endemic species of the coastal region of southeastern Europe, showed a unique chromosome number, 2n = 2x = 20, and bimodal karyotype with one large and nine smaller pairs of chromosomes. Staining with fluorochromes CMA3 (chromomycin A3) and DAPI (4,6-diamidino-2-phenylindole) revealed heterochromatic regions associated with NORs, centromeres, and several interstitial heterochromatic bands on the longest chromosome pair. Double-target FISH with two ribosomal DNA probes revealed one locus of 5S rRNA genes in the pericentromeric region of chromosome pair 3 and one locus of 18S-5.8S-26S rRNA genes on the short arm of chromosome pair 4 in all plants and populations analyzed. Southern hybridization analysis and FISH experiments demonstrated that the distal ends of H. dalmatica chromosomes contain the vertebrate telomere (5'-TTAGGG-3') repeat type rather than the Arabidopsis (5'-TTTAGGG-3') heptamer, and so suggest that this Asparagales species along with Aloe and Othocallis contains the vertebrate-type telomere repeat.

Animals↗