The effect of sodium iodate on the ciliary body/iris in rabbits.
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A widely used method for analyzing hydrolyzable tannins afer reaction with KIO(3) has been modified to include a methanolysis step followed by oxidation with KIO(3). In the new method, hydrolyzable tannins (gallotannins and ellagitannins) are reacted at 85 degrees C for 20 h in methanol/sulfuric acid to quantitatively release methyl gallate. Dried plant samples can be methanolyzed under the same conditions to convert hydrolyzable tannins to methyl gallate. Oxidation of the methyl gallate by KIO(3) at pH 5.5, 30 degrees C, forms a chromophore with lambda(max) 525 nm, which is determined spectrophotometrically. The detection limit of the method is 1.5 microg of methyl gallate, and with plant samples, relative standard deviations of less than 3% were obtained.
In the Xinjiang province of western China, conventional methods of iodine (I) supplementation (i.e, goiter pills and iodinated salt) used to mitigate I deficiencies were ineffectual. However, the recent addition of KIO3 to irrigation waters has proven effective. This study was conducted to determine the effects of I form and concentration on rice (Oryza sativa L.) growth, I partitioning within the plant, and ultimately to assist in establishing guidelines for incorporating I into the human food chain. We compared IO3- vs. I- in order to determine how these chemical species differ in their biological effects. Rice was grown in 48 L aerated tubs containing nutrient solution and IO3- or I- at 0, 1, 10, or 100 micromoles concentrations (approximately 0, 0.1, 1, and 10 mg kg-1 I). The IO3- at 1 and 10 micromoles had no effect on biomass yields, and the 100 micromole treatment had a small negative effect. The I- at 10 and 100 micromoles was detrimental to biomass yields. The IO3- treatments had more I partitioning to the roots (56%) on average than did the I- treatments (36%), suggesting differences in uptake or translocation between I forms. The data support the theory that IO3- is electrochemically or biologically reduced to I- prior to plant uptake. None of the treatments provided sufficient I in the seed to meet human dietary requirements. The I concentration found in straw at 100 micromoles IO3- was several times greater than seed, and could provide an indirect source of dietary I via livestock feeding on the straw.
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The electroretinogram (ERG) was recorded in 15 ICR albino mice before and 2, 4 and 24 h after injection of 40 mg/kg of NaIO3 and the results were compared with the corresponding ultrastructural findings. Both a- and b-wave amplitudes decreased at 2 h after injection and increased at 4 h to the levels before injection and further increased at 24 h after injection. The b-wave threshold intensity increased by 2.1 log units at 2 h after injection. Thereafter, it decreased once at 4 h and significantly increased at 24 h by 3.4 log units. Electron microscopic examination showed swollen cytoplasmic organelles of the retinal pigment epithelial cells at 4 h after injection. It was thus demonstrated that an early effect of NaIO3 on the electrical response of the mouse retina was more conspicuous than histopathological changes.
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