[Studies of the microflora from the female genital tract. I. Incidence of fungi in the female genital tract in normal and pathological states].
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Candida↗
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[Surgical treatment of abnormalities of the external genital organs in girls affected with adreno-genital syndrome].
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Adrenal Hyperplasia, Congenital↗
Effect of placement of epididymis of orchidectomized rats in the abdomen on spermatozoa in the genital tract & accessory genital organs.
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Abdomen↗
[Genital bowenoid papulosis. Pseudo-Bowen's disease of the genital area].
This report deals with a new disease, which is characterized by multiple reddish papules on the genitalia of young adults. The histological picture shows changes of squamous cell carcinoma in situ. The possible etiology is discussed. It is too early to predict the biological behaviour of these lesions.
Adult↗
[Value of postoperative urodynamic assessment for long-term urinary function results after treatment of genital prolapse. 103 examinations after treatment of genital prolapse].
OBJECTIVE: To evaluate the predictive value of postoperative urodynamic assessment on the apparition or the resurgence of genuine stress incontinence after a surgical procedure for genitourinary prolapse. SUBJECTS: 103 patients operated on for prolapse, with or without an associated surgical procedure for genuine stress incontinence. A review of the results of the urodynamic assessment carried out during the early post operative period was effected. RESULTS: 77.7% of the patients had an associated procedure for stress incontinence during surgery for prolapse. An urodynamic abnormality such as intrinsic sphincter deficiency and/or transmission ratio default was noted in 83.3% of the women who demonstrated incontinency during postoperative assessment, and in 76.7% of the patients without any problem of continence. During long term follow up, only 41.7% of the women who were incontinent shared intrinsic sphincter deficiency, and an abnormal cytometric parameter was noted in 74.4% of continent patients. 86% of the patients who were incontinent in the early postoperative period will remain so, whatever the result of the cystometric evaluation. CONCLUSION: Our results show that there is no correlation between the various cystometric parameters evaluated during the postoperative period, and the symptoms described by the patients. The absence of abnormal urodynamic assessment cannot consistently predict normal bladder function. Only postoperative symptoms can be considered to be predictive of a satisfactory surgical cure.
Adult↗
Genital injuries in women reporting sexual assault.
BACKGROUND: The likelihood of genital injury following sexual assault remains unclear. Genital injury related to sexual assault is often an issue in court proceedings, with the expectation that injuries will be found in 'genuine' cases. Conviction rates are higher when the complainant has genital injuries. OBJECTIVES: To determine the type, frequency and severity of genital and non-genital injuries of women following alleged sexual assault and, in addition, to determine factors associated with the presence of injuries. METHODS: The assault records and forensic examination findings of 153 consecutive women who attended a sexual assault service in Newcastle, Australia, between 1997 and 1999 were reviewed. All of the women were examined within 72 h of the assault. Associations were sought between victim and reported assault variables and the presence of injury using multiple logistic regression analysis. RESULTS: Of the women, 111 (73%) were aged under 30 years and only 4% were over 50 years. Penile-vaginal penetration was the most common type of sexual assault (86%). Non-genital injuries were found in 46% of the women examined (mostly minor) and genital injury in only 22%. Genital injury in the absence of non-genital injury was rare (3%). Independent risk factors for the detection of non-genital injuries were reported threats of violence (OR 5.7, 95% CI; 2.2-14.6). Risk factors for genital injury were the presence of non-genital injury (OR 19, 95% CI; 6.0-63.0), threats of violence (OR 3.7, 95% CI; 1.5-8.9) and being over the age of 40 years (OR 5.6, 95% CI; 1.6-20.3). If the alleged assailant was known to the woman this was protective for both non-genital (OR 0.3, 95% CI; 0.1-0.5) and genital (OR 0.4, 95% CI; 0.2-0.9) injury. CONCLUSIONS: The presence of genital injury should not be required to validate an allegation of sexual assault, particularly in the absence of non-genital injuries.
Adolescent↗
Current diagnostic techniques in genital herpes: their role in controlling the epidemic.
Genital herpes continues to be a public health problem in both developed and developing countries. Laboratory confirmation of clinical diagnosis is important, particularly as there are other conditions which present similarly to genital herpes, while atypical presentations of genital herpes also occur. Herpes simplex virus type 2 (HSV-2) is the most common cause of genital herpes globally, although HSV-1 genital herpes infections occur also. Type-specific serology has overcome the technical problems posed by earlier cross-reactive HSV serological assays, hence HSV-2 specific antibodies can be identified using both in-house and commercial assays. All HSV-2 serological assays require an appropriately thorough validation, and here an understanding of the natural history of genital HSV infection is important. Validated HSV-2 specific antibody assays have featured in seroepidemiological studies which have emphasised the largely asymptomatic nature of this infection. Subsequent seroepidemiological studies have included a sexual lifestyle questionnaire to identify risk factors for genital HSV-2 infection. This has given rise to serological screening proposals which, it is argued, may arrest the spread of genital herpes in the general population. However, counter arguments to such proposals are important to consider. As regards diagnosis and management of genital herpes in individual patients, situations have been identified where type-specific serology may be of benefit. PCR has become the method of laboratory diagnosis for HSV encephalitis over the past decade, but its role in the diagnosis of genital herpes has been addressed only in recent years. Evaluation of HSV PCR on specimens from genital herpes cases has shown PCR to be more sensitive than virus culture, the traditional "gold standard" of HSV identification. However, questions remain regarding the acceptance of HSV into routine diagnostic settings, particularly concerning sample preparation, although automation and the ability to include diagnosis of other genital infections in a multiplex PCR is an advantage. Such developments should enhance the role of PCR in genital herpes diagnosis and ultimately reduce costs relative to traditional methods such as culture and HSV antigen detection. Finally, the use of type-specific serology and HSV PCR in genital herpes research is noted.
Antibodies, Viral↗
Development of clinically recognizable genital lesions among women previously identified as having "asymptomatic" herpes simplex virus type 2 infection.
STUDY OBJECTIVE: To determine if patients initially identified by history, clinical examination, and serologic status as having asymptomatic herpes simplex virus type 2 infection report clinically recognizable genital lesions after having a detailed instructional session on the clinical signs and symptoms of genital herpes. DESIGN: Prospective follow-up of two groups of women. SETTING: Outpatient clinic of an urban city-county hospital. PATIENTS: Two populations of women were recruited. One group was referred because they had documented asymptomatic genital herpes or were suspected of asymptomatically transmitting genital herpes to a sex partner. The other group was recruited from the hospital's general gynecologic clinic and consisted of women with herpes simplex virus type 2 antibodies who denied a history of genital herpes. INTERVENTION: Patients had a one-on-one interview describing the clinical symptoms of genital herpes. MEASUREMENTS AND MAIN RESULTS: Twelve of nineteen women with herpes simplex virus type 2 antibodies who had documented asymptomatic genital herpes or were suspected of asymptomatically transmitting infection to a sex partner, and 18 of 43 women with herpes simplex virus type 2 antibodies who were recruited from the gynecologic clinic developed clinically recognizable genital lesions during a 5-month follow-up. Symptomatic genital herpes was the most frequent gynecologic complaint identified during follow-up. CONCLUSIONS: Approximately 50% of women with herpes simplex virus type 2 antibodies who do not initially report a history of genital lesions do indeed have clinically symptomatic genital herpes. These women can be accurately counseled and taught to distinguish genital herpes from other genitourinary infections. Identification of symptomatic recurrences and counseling to avoid sexual activity during these episodes may help reduce transmission of genital herpes.
Adolescent↗
[Risk factors for Clamydia infections of the genital organs in adolescent females].
INTRODUCTION: Chlamydia trachomatis (Ct) infections are the most common bacterial sexually transmitted diseases (STDs). The highest age-specific rates for chlamydia are found in adolescents. Female adolescents are more susceptible to STDs than older women because their cervical anatomic development is incomplete and especially sensitive to infection by certain sexually transmitted pathogens, and for some other features that characterize sexual behavior and health care behavior of the young people. The aim of this study was to identify risk factors and risk indicators statistically associated with the presence of Ct genital infection in adolescent females. METHOD: The study group comprised 300 sexually active 19 years old girls who were attending the Mother and Child Health Care Institute of Serbia in the period from 1995 to 1997. The participants of this study were interviewed about their sexual behavior, health care behavior and their responsibility level in sexual relationships. Physical examination included microbiological and colposcopic findings. Vaginal and cervical microbiological examinations included the analysis of collected specimens for microscopic and culture analyses. Ct infection of the cervix was identified by direct immunofluorescence staining of smears using monoclonal antibodies. Colposcopic findings were divided in two groups: cervical ectopy and other findings. Data were statistically analyzed using step-wise linear regression. RESULTS: The prevalence of Ct genital infection in the study group was 30.3%. Characteristics in adolescent girls with this infection were mostly as follows: first sexual intercourse was before the age of 17, first sexual partner was older, a great number of sexual partners, (more than 3-25.3% vs 20.1%; more than 4-16.5% vs 10.5%), sexual intercourse during casual contact (44.0% vs 34.4%) and high coital frequency (83.5% vs 72.7%). Among these characteristics, only the coital frequency was significantly associated with the probability of chlamydial genital infection (p < 0.05). Statistical findings about the use of contraceptives among the adolescents indicated that the level of safe sexual practice was low in the majority of girls, despite the presence of chlamydial genital infection. Nevertheless, the girls without Ct genital infection more frequently reported the condom as the method of contraception used for the longest adolescence period (41.6%) in comparison with the adolescent females infected by Ct (17.6%). This difference was statistically significantly associated with the possible manifestation of Ct genital infection (p < 0.05). Healthy adolescent females used condom more frequently at the last intercourse (37.3%) than girls with Ct genital infection (18.2%). Adolescent females often experienced only 0-2 methods of contraception (70.3% of girls infected with Ct and 59.8% of healthy girls). Health care behavior was poor in the examined girls. The suspicion of getting a STD was the reason for their first gynaecological examination in 25.3% of adolescent women with Ct genital infection and in 20.6% of healthy girls. Unwanted pregnancy during adolescence reported 16.5% of girls with Ct cervicitis and 15.8% of healthy girls. The existence of STD previous to examination was noted in 7.9% of infected girls and 7.7% of healthy subjects. Drug abuse reported 23.1% of studied adolescent females with Ct genital infection and 23.0% without it. A large proportion of both the adolescent females and their partners had negative attitudes toward the condom use (54.9% of infected girls and 57.1% of their partners; 32.1% of healthy girls and 35.9% of their partners). The difference between the attitudes toward the condom use among infected and healthy adolescent women and their partners was statistically significant (p < 0.001). Clinical findings of cervical ectopy were more frequent in girls infected with Ct (60.4%) in comparison to healthy girls (15.8%). These differences were also statistically significant (p < 0.001). Associated genital infections (vaginal and/or cervical) were more frequent in adolescent women with Ct cervicitis (56.0%) than in healthy girls (36.8%). The observed differences were statistically significant (p < 0.01). CONCLUSION: Predictors of Ct genital infection in adolescent women, according to the step-wise linear regression analysis, were as follows: the presence of cervical ectopy, negative attitudes of adolescent girls and their partners toward the condom use; associated genital infections; frequent coitus; experience in causal sexual relations and older age of the first sexual partner. However, the possibility that this criterion provides imprecise identification of uninfected adolescent women and separation of only Ct infected women, is limited. Therefore, screening of the presence of Ct genital infections in all sexually active adolescent women is necessary.
Adolescent↗
[Genital herpes with special emphasis on perinatal herpes simplex virus infection].
INTRODUCTION: The incidence of genital herpes is increasing worldwide and at present herpes simplex virus type 2 is the most common cause of genital ulceration all over the world. CLASSIFICATION: The International Herpes Management Forum (IHMF) was established in 1993, suggesting a new classification of genital herpes: primary genital herpes, non-primary genital herpes, recurrent genital herpes, first episode genital herpes, atypical genital herpes and asymptomatic HSV (herpes simplex virus) infection. DIAGNOSIS: Clinical diagnosis of genital herpes should be confirmed by laboratory techniques, whereas a positive HSV culture is the best test for confirming the clinical diagnosis. Serological testing, including Western blot assay, is not the method of choice for diagnosis genital herpes. THERAPY: Management of patients with genital herpes must include various antiviral drugs (acyclovir, valacyclovir, famiclovir), but also must take into consideration the patients' clinical and emotional issues. Patients with few recurrences are best managed with episodic antiviral therapy, but those with more frequent recurrences may find a long term suppressive therapy more beneficial. Herpes simplex virus is acquired during labor in about 90% of neonatal herpes virus cases with direct contact with infected maternal genital secretions, in 5% of cases in utero (ascending infection or transplacentary) and in another 5% of cases HSV is acquired post partum. Herpes simplex virus infection includes skin infection, eye and mouth manifestations, CNS diseases and disseminated disease with multiorgan involvement. CONCLUSION: In order to reduce the risk of HSV transmission to the infant IHMF has suggested management of pregnant women with primary genital herpes: delivery by Caesarean section between 34th week and term. Acyclovir treatment may reduce the viral load at delivery, but before this can generally be recommended, more data are still required.
Female↗
Sonic hedgehog signaling from the urethral epithelium controls external genital development.
External genital development begins with formation of paired genital swellings, which develop into the genital tubercle. Proximodistal outgrowth and axial patterning of the genital tubercle are coordinated to give rise to the penis or clitoris. The genital tubercle consists of lateral plate mesoderm, surface ectoderm, and endodermal urethral epithelium derived from the urogenital sinus. We have investigated the molecular control of external genital development in the mouse embryo. Previous work has shown that the genital tubercle has polarizing activity, but the precise location of this activity within the tubercle is unknown. We reasoned that if the tubercle itself is patterned by a specialized signaling region, then polarizing activity may be restricted to a subset of cells. Transplantation of urethral epithelium, but not genital mesenchyme, to chick limbs results in mirror-image duplication of the digits. Moreover, when grafted to chick limbs, the urethral plate orchestrates morphogenetic movements normally associated with external genital development. Signaling activity is therefore restricted to urethral plate cells. Before and during normal genital tubercle outgrowth, urethral plate epithelium expresses Sonic hedgehog (Shh). In mice with a targeted deletion of Shh, external genitalia are absent. Genital swellings are initiated, but outgrowth is not maintained. In the absence of Shh signaling, Fgf8, Bmp2, Bmp4, Fgf10, and Wnt5a are downregulated, and apoptosis is enhanced in the genitalia. These results identify the urethral epithelium as a signaling center of the genital tubercle, and demonstrate that Shh from the urethral epithelium is required for outgrowth, patterning, and cell survival in the developing external genitalia.
Animals↗
Isolation of herpes simplex virus from the genital tract during symptomatic recurrence on the buttocks.
OBJECTIVE: To estimate the frequency of isolation of herpes simplex virus (HSV) from the genital tract when recurrent herpes lesions were present on the buttocks. METHODS: Data were extracted from a prospectively observed cohort attending a research clinic for genital herpes infections between 1975 and 2001. All patients with a documented herpes lesion on the buttocks, upper thigh or gluteal cleft ("buttock recurrence") and concomitant viral cultures from genital sites including the perianal region were eligible. RESULTS: We reviewed records of 237 subjects, 151 women and 86 men, with a total of 572 buttock recurrences. Of the 1,592 days with genital culture information during a buttock recurrence, participants had concurrent genital lesions on 311 (20%, 95% confidence interval [CI] 14-27%) of these days. Overall, HSV was isolated from the genital region on 12% (95% CI 8-17%) of days during a buttock recurrence. In the absence of genital lesions, HSV was isolated from the genital area on 7% (95% CI 4%-11%) of days during a buttock recurrence and, among women, from the vulvar or cervical sites on 1% of days. CONCLUSION: Viral shedding of herpes simplex virus from the genital area is a relatively common occurrence during a buttock recurrence of genital herpes, even without concurrent genital lesions, reflecting perhaps reactivation from concomitant regions of the sacral neural ganglia. Patients with buttock herpes recurrences should be instructed about the risk of genital shedding during such recurrences. LEVEL OF EVIDENCE: II-2.
Adolescent↗
Increasing prevalence of genital herpes in developing countries: implications for heterosexual HIV transmission and STI control programmes.
BACKGROUND: The recognition that sexually transmitted infections (STI) facilitate HIV transmission among heterosexuals has led to a rejuvenated focus on improving STI control as a component of HIV prevention programmes in developing countries. While efforts so far have focused mainly on all STI, there is increasing evidence that genital ulcers facilitate a considerable proportion of HIV transmission among heterosexuals and that this effect has been underestimated. This paper focuses on the epidemiology of genital herpes in developing countries past and present. OBJECTIVES: To review the scientific literature about the epidemiology of genital herpes in developing countries and discuss the implications of the findings for STI control and HIV prevention programmes. SEARCH METHODOLOGY: A Medline search for June 1966 to August 1999 using the keywords, genital herpes, STD and developing countries, and genital ulcers in MeSH and free text. Abstract books from recent international AIDS conferences and other international STD conferences were reviewed. The annual reports of the medical officers of heath for Harare 1982-1998 and Durban 1989-1997 were reviewed to detect trends in genital herpes diagnoses. FINDINGS: Genital herpes, formerly regarded as a minor STI in most developing countries, has now emerged as a leading cause of genital ulceration in many countries where syphilis and chancroid were more prevalent previously. This increased recognition of genital herpes reflects both a change in the pattern of STI epidemiology through a decline in syphilis and chancroid as a response to HIV control programmes and improved techniques for diagnosing herpetic infection. Countries with significant heterosexual HIV epidemics also appear to have rapidly increasing numbers of genital herpes cases. CONCLUSIONS: The emergence of this herpes epidemic must be addressed through innovative strategies that will be viable, sustainable, acceptable, and effective in developing countries. In countries where genital herpes is a significant problem, local adaptation of WHO treatment algorithms should be made. STI service providers should be trained about issues around the transmission of herpes and how best to advise clients about dealing with, and recognising, recurrences. The effectiveness of antiviral treatment for genital herpes should be investigated in core groups at high risk of HIV.
Developing Countries↗
Epidemiology of genital herpes infections.
Much has been learned in the last decade about the epidemiology of genital herpes infections, including new information about seroprevalence and the risk of transmission of genital herpes to sex partners and at delivery. Unfortunately, the type-specific serologic assays now routinely used in these studies are not widely available, and commercially available assays that claim to be type-specific are not. Thus, most clinicians still do not have access to reliable type-specific assays. In cross-sectional seroprevalence studies, detection of HSV-2 antibody is positively associated with increasing age, lower levels of income or education, increased numbers of sexual partners, black or Hispanic race, female gender, male homosexual activity, and HIV infection. In addition, studies cited in this review have clarified the clinical spectrum of genital herpes infection in persons who have transmitted genital herpes to a sex partner, have shed virus asymptomatically, or are found to have HSV-2 antibody. Ten percent to 40% of these individuals are aware that they have genital herpes, whereas the remaining 60% to 90% are not. Among the latter, at least half have a history of recurrent genital lesions typical of genital herpes or can be taught to recognize typical, symptomatic episodes within 6 months if examined promptly after the onset of any unexplained genital symptoms. The remainder, about a third of the total, have no history of genital herpes and remain asymptomatic despite a careful history and follow-up examinations; in women in this group, asymptomatic shedding of HSV can be identified. Atypical lesions appear to play an important but as yet incompletely defined role. Most persons who transmit genital herpes to a sex partner or at delivery do not have a history of lesions at the time of transmission of HSV infection, suggesting that asymptomatic shedding or atypical, unrecognized lesions are responsible for most cases of transmission. In heterosexual couples, the risk of acquisition of HSV-2 infection from a sex partner with genital herpes is lowest in men (less than 5%), higher in HSV-1 seropositive women (less than 10%), and highest (about 30%) in women without antibody to HSV-1 or HSV-2. The risk of transmission to infants exposed to asymptomatic shedding at delivery is low (about 3%) in women with or without a history of genital herpes if HSV antibody of the same type is present in cord blood.(ABSTRACT TRUNCATED AT 400 WORDS)
Ethnicity↗
Epidemiology of genital chlamydial infections in patients with chlamydial conjunctivitis; a retrospective study.
OBJECTIVE: To determine how often chlamydial conjunctivitis is accompanied by a genital chlamydial infection and if there is a correlation between the dominant hand and the eye first infected. METHODS: We retrospectively studied the records of 65 patients with chlamydial conjunctivitis who were referred to the Outpatient Department of Sexually Transmitted Diseases (STD) of the University Hospital Rotterdam by ophthalmologists of the Eye Hospital Rotterdam. The patients have recently been asked by letter if they were left- or right-handed. RESULTS: Twenty of the 37 men (54%) had a positive chlamydial urethral culture. Seventy per cent of these men had no genital symptoms. Eight of the 37 men (22%) had a non-specific urethritis (NSU). Twenty of the 27 women examined (74%) had a positive chlamydial cervical culture. Sixty per cent of these women had no genital symptoms. Eight women with a genital chlamydial infection also had another genital infection. Five women without a genital chlamydial infection had another genital infection. Two women had no genital infection at all. A correlation between the eye infected and left- or right-handedness of the patient could not be found. CONCLUSIONS: A considerable percentage of the patients with a chlamydial conjunctivitis had a concomitant genital chlamydial infection. The majority of them had no genital symptoms. Since patients with chlamydial conjunctivitis and/or their partners possibly have a concomitant genital chlamydial infection, we recommend referral of both patients and sexual partners to an STD clinic for routine examination and systemic treatment when indicated.
Adolescent↗
Seroepidemiological and socioeconomic studies of genital chlamydial infection in Ethiopian women.
OBJECTIVE: To measure the prevalence of chlamydial genital infection in Ethiopian women attending gynaecological, obstetric and family planning clinics; to identify the epidemiological, social and economic factors affecting the prevalence of infection in a country where routine laboratory culture and serological tests for chlamydial species are unavailable; to determine the risk factors for genital chlamydial infection in those with serological evidence of other sexually transmitted diseases. SUBJECTS: 1846 Ethiopian women, outpatient attenders at two teaching hospitals and a mother and child health centre in Addis Ababa, Ethiopia. SETTING: Gynaecological outpatient department, antenatal, postnatal and family planning clinics. METHODS: Sera were tested for type-specific anti-chlamydial antibodies using purified chlamydial antigens (C. trachomatis A-C (CTA-C), C. trachomatis D-K (CTD-K), Lymphogranuloma venereum (LGV1-3), and C. pneumoniae (CPn)), in a micro-immunofluorescence test. The genital chlamydia seropositivity was analysed against patient's age, clinic attended, ethnic group, religion, origin of residence, age at first marriage and first coitus, income, number of sexual partners, duration of sexual activity, marital status/profession, obstetric and contraceptive history, and seropositivity for other sexually transmitted diseases. RESULTS: Overall exposure to chlamydia species was found in 84%, genital chlamydial infection in 62%, and titres suggestive of recent or present genital infection in 42% of those studied. Genital chlamydial infection was highest (64%) in family planning and lowest (54%) in antenatal clinic attenders. Exposure to genital chlamydia species was influenced by ethnic group and religion. Those married and sexually active under 13 years of age had greater exposure (69%) to genital chlamydial infection than those first sexually active aged over 18 (46%). Prevalence of infection was highest in those with more than five sexual partners (78%) and in bargirls (84%). The lowest income groups had a higher prevalence (65%) of genital chlamydial infection than the wealthiest (48%). Multivariate analysis showed the most important factors to be age at first coitus, religion, prostitution and present age of the woman in that order. Risk for genital chlamydial infection was increased in those with seropositivity for syphilis, gonorrhoea, HSV-2 but not HBV infection. CONCLUSION/APPLICATION: Chlamydial genital infections are highly prevalent in both symptomatic and asymptomatic Ethiopian women. The high prevalence of infection reported reflects a complexity of socioeconomic factors: very early age at first marriage and first coitus, instability of first marriage, subsequent divorce and remarriage or drift into prostitution, all of which are influenced by ethnic group, religion and poverty--together with transmission from an infected group of prostitutes by promiscuous males to their wives, lack of diagnostic facilities and inadequate treatment of both symptomatic and asymptomatic men and women. The problem of chlamydial disease in Ethiopia needs to be addressed urgently in the context of control of STD.
Adult↗
Molecular epidemiology of herpes simplex virus type 1 genital infection in association with clinical manifestations.
The antigenic types of herpes simplex virus (HSV), HSV-1 and HSV-2 are considered to be the etiology of genital herpes. Symptoms of primary HSV-1 and HSV-2 genital infections are similar, however, recurrence of the infection is less frequent after the HSV-1-related genital infection. We determined genotypes of 79 HSV-1 strains isolated from genital lesions in women (43 from primary and 36 from recurrent infections), by analyzing restriction fragment length polymorphism of the HSV-1 strains. Each proportion of genotypes of F1, F12, and F41 in strains isolated from recurrent genital lesions was higher than the corresponding proportion in strains from primary genital lesions. Genotypes of HSV-1 strains isolated two or more times from recurrent genital lesions of each of three subjects were genotype F1, thereby supporting the hypothesis that the F1 genotype is closely associated with recurrence. While the possibility of a genotype preference at the site of infection was not ruled out, genotypes of more than half the number of HSV-1 strains from genital lesions were the same as those from non-genital (mainly oral-facial) lesions analyzed in our foregoing studies, thus indicating that most HSV-1 genotypes are apparently shared by genital and non-genital lesions.
Adolescent↗
A comparison between detailed and simple histories in the diagnosis of genital herpes complicating pregnancy.
OBJECTIVE: All women seropositive for herpes simplex virus-2 are at risk for asymptomatic viral shedding at the onset of labor and neonatal transmission of the virus. Unfortunately, only 20% to 35% of seropositive adults give a history consistent with genital herpes. We evaluated whether more detailed questioning during pregnancy might elucidate symptoms predictive of seropositivity and therefore better identify women at risk for herpes simplex virus shedding at delivery. STUDY DESIGN: During pregnancy 201 randomly selected women were asked in-depth questions about recurrent genital symptoms and whether they "currently have or have ever had genital herpes." An assessment was made whether the patient had a history compatible with genital herpes. This assessment and the "yes/no" history of genital herpes obtained by physicians at the initial prenatal visit were both compared with herpes simplex virus serologic studies by Western blot. RESULTS: Of 201 patients interviewed, 177 gave no history of genital herpes. Of these, 30.4% were seropositive for herpes simplex virus-2. Detailed histories on these 177 patients indicated that among the 159 subjects without suggestive symptoms or with somewhat suggestive symptoms the corresponding rates of seropositivity were 28% and 30%. Among the 18 (10.2%) subjects with highly suggestive symptoms, only 50% were seropositive. The positive predictive values for recurrent genital symptoms to predict herpes simplex virus-2 seropositivity ranged from 30% to 57%. CONCLUSION: A detailed history of genital symptoms is no better at identifying an herpes simplex virus-2 seropositive patient than is simply asking if she has ever had genital herpes. Serologic screening is a more accurate method of identifying women with past genital herpes or those who are at risk for acquiring genital herpes during pregnancy.
Adolescent↗