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At least 145 records · Page 8Linked to original sources
PubMed1976

Electron microscopic observations of FL cells infected with herpes simplex virus. III. Dense bodies.

FL cells infected with the -GCr Miyama strain of herpes simplex virus at an adsorbed multiplicity of approximately 10 were fixed at late stages of infection and examined by electron microscopy. Dense bodies containing electron-dense material and surrounded by a limiting membrane were occasionally observed in the perinuclear disternae and in intranuclear vacuoles. Budding of electron-dense material to the cisternae with acquisition of a limiting membrane at the inner nuclear membrane was also occasionally observed. These findings are in constrast with observations that in cells infected with cytomegalovirus numerous dense bodies and their budding process were observed only in the cytoplasmic area.

Amnion↗
PubMed1978

Cytological analysis of bone marrow present in the bone nodules induced by human FL cells in mice.

Bone marrow cells recovered from bone nodules induced in mice by intramuscular transplantation of human FL cells were compared with the host femoral bone marrow. The precursors and mature forms of all types of blood cells were found in the induced bone marrow. The concentration of lymphocytes was significantly higher, and that of erythropoietic cells significantly lower in the induced bone marrow as compared with heterotopic femoral bone marrow.

Animals↗
PubMed1986

[Cellular ultrastructure of the meningococcus when incubated in a continuous culture of human FL-line amnion].

Some details of the ultrastructure of several meningococcal strains having had contacts with cells in continuous human amnion cell culture FL for 6 hours to 2 days have been defined with greater precision by means of electron microscopy. The study has shown that the contact of meningococci with the tissue culture is accompanied by the appearance of meningococcal forms with the defective cell wall, similar to L-forms: spheroplast, protoplast, gigantic cells and microcells, as well as budding variants. The meningococcal variants with the defective cell wall, appearing in the cell culture, and the forms occurring (in different proportions) in "ripe" meningococcal populations developing in the culture media for a long time and isolated from a human body have been found to have no essential differences in their fine structure. These data indicate that any external influences (meningococci are highly sensitive to such influences) produce sufficiently rapid changes, similar to L-transformation, in the fine structure of these microorganisms.

Amnion↗
PubMed1987

Suppression by herbimycin A of 12-O-tetradecanoylphorbol-13-acetate-induced dissociation of cells and morphological changes in cultured FL and MDCK cells.

Human amnion membrane epithelial (FL) and Madin-Darby canine kidney (MDCK) cells formed colonies which consisted of tightly packed cells. A bundle of actin filaments in the cells at the edge of the colonies ran parallel to the outline of these cells. The cells were attached to the substratum by regions which were observed as black solid lines by interference-reflection microscopy. Treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) for 24 hr induced dissociation of and morphological changes in the cells in the colonies. TPA also disrupted the organization of the actin filaments and the attachment of cells to the substratum. Addition of herbimycin A for 24 hr inhibited the TPA-induced dissociation of the cells in colonies. Treatment with herbimycin A also inhibited TPA-induced modulation of the organization of actin filaments and of the adhesion of cells to the substratum. However, herbimycin A did not inhibit the rapid spreading of cells induced by TPA in the absence of serum. These results suggest that herbimycin A inhibits the dissociation of cells and the morphological changes induced by TPA through suppression of the disruption of a bundle of actin filaments and modulation of adhesion of cells to the substratum.

Actins↗
PubMed1977

Automated sequencing of insoluble peptides using detergent. Bacteriophage fl coat protein.

Peptides which are highly nonpolar and insoluble under moderate conditions of pH and ionic strength cannot be subjected to automated sequence analysis. We report a method for solubilization of one such peptide, bacteriophage fl coat protein, by chemical modification in the presence of sodium dodecyl sulfate. Following this treatment the 50-residue peptide was degraded stepwise in an automated sequenator using a single cleavage Quadrol program with high repetitive yield through residue 47. We also report a modified program using detergent incorporated into dimethylallylamine buffer which permitted sequencing with high repetitive yields for at least the first 18 residues of the unmodified and otherwise highly insoluble coat protein. The presence of detergent caused no observable difficulties in detection of residues by gas chromatography, thin layer chromatography, or amino acid analysis.

Amino Acid Sequence↗
PubMed1986

Membrane hyperpolarization following chronic exposure of FL-cells to low doses of ouabain.

The transmembrane potential (TMP) of FL-cells (monolayer cultures) in Eagle's medium with ouabain concentrations from 10(-4) to 10(-11) M was measured by means of glass microelectrodes over 96 h. High ouabain concentrations caused total depolarization of the cell membrane with subsequent cell death after 24 to 48 h. A level of 10(-7) M ouabain was tolerated by the cells over 96 h, and the TMP was decreased about 50% vs. controls in ouabain-free medium. Ouabain at concentrations from 10(-11) to 10(-8) M increases TMP by 20-40%, which decayed after 24 to 48 h. The low glycoside concentrations are assumed to cause, if only part of the glycoside receptors (Na+ pumps) is blocked, an activation of the still active pumps (turnover) and enhanced formation of transport enzymes and their incorporation into the membrane (replacement) so that the overall result is a stimulation of the cell membrane transport systems.

Cell Line↗
PubMed1980

Transcription of bacteriophage fl. The major in vivo RNAs.

We have analyzed eight major phage-specific mRNA species which are synthesized following infection of Escherichia coli with bacteriophage fl. The approximate half-lives of these RNAs appear to be inversely proportional to their lengths. Three species have the properties of primary transcripts. They are labeled very rapidly with [3H]uracil, and they co-migrate by sodium laruyl sulfate-urea polyacrylamide gel electrophoresis with the three major RNAs transcribed from replicative form DNA in vitro using [gamma-32P]GTP as the labeled ribonucleoside triphosphate. At least three of the remaining RNAs synthesized in vivo arise by some type of processing reaction. The processed species contain, as determined by oligonucleotide analysis, the information for the gene VIII protein and the 3'-terminal oligonucleotide expected for a transcription termination event at the rho-independent site following gene VIII. The two primary transcripts which could be analyzed also have this same 3'-terminal oligonucleotide. These results suggest that the processed mRNAs are the 3'-terminal cleavage products of primary transcripts which had terminated at the rho-independent site.

Coliphages↗
PubMed1980

Inhibitory activity of 2-aminooxazole derivatives against Coxsackie B1 virus in FL cells.

The antiviral activity of three-2-aminooxazoles was studied against coxsackie B1 virus in FL cells. The virus-induced cytopathic effect was nearly completely suppressed by two of the test compounds as revealed by spectrophotometric turbidity measurements. The plaque reduction amounted to 95--100 per cent. The compounds had no effect on extracellular virus or on absorption and penetration. The inhibition of infectious virus yield in one-step growth experiments reached 94--99.9 per cent. One of the compounds showed no inhibition of cellular RNA synthesis, whereas viral RNA synthesis was completely suppressed. The other two exerted only a small influence on cellular RNA synthesis. However, the results suggested an inhibition of uridine transport.

Amnion↗
PubMed1983

[Antigen isolation from Rickettsia prowazekii cultured in FL cells and its use in the macrophage migration inhibition reaction].

An antigen obtained from R. prowazekii cultivated in hydrocortisone-treated FL-cells possesses activity determined in the complement fixation test and the macrophage migration inhibition test. Such antigen can be used in the macrophage migration inhibition test with macrophages obtained from animals immunized with the preparations of rickettsial egg cultures.

Amnion↗
PubMedDate not supplied

[Complex of single-stranded phage DNA and protein--a product of bacteriophage fl gene 3. Distribution of gene 3 protein in the DNA molecule].

Upon a 50% isopropanol treatment of phage fl in a 1 M NaCl solution protein A (gene 3 product)--DNA complex is precipitated while protein B (gene 8 product) was still solubilized. After such a treatment the DNA--protein complex containing 10--40% of protein A and less than 0.0025% of protein B was obtained. Evidence was obtained that there was no non-specific rearrangement of protein A during the isolation procedure. The complex was treated with endonuclease R.HAC III, followed by electrophoresis of the resulted fragments and estimation of the [14C] protein A (labeled with [14C]histidine) throughout the gel. The maximal radioactivity coincided with the DNA bands, being proportional to the DNA content in the respective bands. The data obtained indicate that protein A is iniformly arranged along the DNA molecule.

Chemical Phenomena↗
PubMed1980

Isolation and characterization of the C and D proteins coded by gene IX and gene VI in the filamentous bacteriophage fl and fd.

The C and D proteins from bacteriophage fd and fl have been purified and characterized. Since the DNA sequence is known, the amino acid composition of these purified proteins indicated that they are coded for by the phage, the C protein being the product of Gene IX and the D protein specified by Gene VI. The molecular weights of the C and D proteins were calculated from the DNA sequences to be 3,650 and 12,350, respectively. These values are close to the molecular weights observed after polyacrylamide gel electrophoresis of the proteins in sodium dodecyl sulfate. Since the C and D proteins can be selectively labeled with radioactive cysteine and arginine, it was possible to estimate the number of the C and D protein molecules relative to the number of A protein molecules which had been accurately determined in previous studies. Based on these results, the average phage particle contains 5 A, 5 D, and 10 C protein molecules.

Amino Acids↗
PubMed1981

Structure of the filamentous bacteriophage fl. Location of the A, C, and D minor coat proteins.

The location within the virion of the A, C, and D minor coat proteins of the filamentous bacteriophage fl has been analyzed. The A protein is present in approximately 5 copies/particle and is located at the tip of normal length phage, miniphage, and fl/pBR322 chimeric phage, a longer than normal length phage. The mole ratios of the A, C, and D proteins are the same for each type of particle, consistent with a model of phage organization in which the minor coat proteins are clustered near or at the ends of the phage. Normal length phage were fragmented by passing them through a French press, and those fragments that contained the A protein were separated from those that did not by treating the mixture with anti-A protein antibody. Analysis of the protein compositions of the two populations of fragments showed that the A and D proteins were found together in one population of fragments and that most, it not all, of the C protein was found in the other. These results show that the D protein is located near or at the A protein end of the phage and that the C protein is located in a region near or at the opposite end. Treatment of the virion with proteases which lowered the infectivity of the phage resulted in particles in which only the A protein was cleaved to any detectable extent. These particles remained resistant to the action of micrococcal nuclease.

Capsid↗
PubMed2003

Determination of methamphetamine and amphetamine in abusers' plasma and hair samples with HPLC-FL.

This paper describes highly sensitive HPLC methods for the determination of amphetamine (AP) and methamphetamine (MP) in abusers' plasma and hair samples. AP and MP were derivatized with the fluorescent reagent, DIB-Cl, to yield a highly fluorescent DIB-derivatives of AP and MP, which were then analyzed by HPLC with fluorescence detection at excitation and emission wavelengths of 325 and 430 nm, respectively. The separation was achieved on an ODS column with isocratic mobile phases composed of acetoniltrile and citrate buffer (55:45, v/v) for plasma samples and of acetonitrile-methanol-citrate buffer (45:20:37.5, v/v/v) for hair samples. The limits of detection were less than 0.87 ng/mL and 0.12 ng/mg in plasma and hair samples, respectively, for both AP and MP. The methods were then applied to the determination of MP and its metabolite AP in plasma obtained from two cases of illegally ingested MP and in one of the cases' hair received later. Case I was treated with dialysis; samples before and after dialysis were analyzed by the described method. After dialysis for 5 h, the total plasma levels of AP and MP decreased from 720 to 190 ng/mL. For case II, MP and AP levels were monitored for 3 days after digestion. Total plasma levels decreased from 57 ng/mL in the day of digestion to 11 ng/mL after 3 days. In hair samples, AP and MP could also be detected in very low concentrations.

Adult↗
PubMed2005

Determination of zolmitriptan enantiomers in rat liver microsomes by chiral high performance liquid chromatography with fl uorescence detection.

A selective chiral high performance liquid chromatographic method was developed and validated to separate and quantify the enantiomers of a new potent selective 5-HT(1B/1D) receptor partial agonist, S-zolmitriptan, and its antipode in rat liver microsomes induced with beta-naphtho flavone. S- and R-zolmitriptan were extracted from rat hepatic microsomal incubates with chloroform/isopropanol (75:25, v/v), and were separated on a narrow-bore enantioselective normal phase Chiralpak AD-H column (250 x 0.46 mm) with hexane-isopropanol-triethylamine (72/28/0.25, v/v/v) as mobile phase and fluorescence detection with emission at 350 nm and excitation at 291 nm. The calibration curves were linear for R- and S-zolmitriptan concentration over the range 0.1-5.0 microg/mL (r = 0.9996 and 0.9999), and the limits of quantitation were 0.1 microg/mL. The metabolism and interaction of the enantiomers of zolmitriptan in treated hepatic microsomes were investigated using chiral HPLC. There was significant difference between the disposition of the S- and R-zolmitriptan when racemic zolmitriptan or single enantiomers of zolmitriptan were incubated for 5, 10 and 20 min, suggesting that the metabolism of zolmitriptan in rat liver microsomes is enantioselective. In addition, there was also a significant difference between the IC(50) of R- to S-zolmitriptan and S- to R-zolmitriptan (IC(50S/R)/IC(50R/S) = 45.2). This indicated that the disposition process favored the S-form of zolmitriptan.

Animals↗
PubMed2003

Protective effect of Astragali radix extract on interleukin 1beta-induced in fl ammation in human amnion.

The aim of this study was to investigate the effect of Astragali radix extract on interleukin (IL)-6 and tumour necrosis factor (TNF)-alpha production and prostaglandin E(2) (PGE(2)) and leukotriene C(4) (LTC(4)) release from IL-1beta-stimulated human amnion. Primary monolayer cultures of amnion cells were established from women undergoing elective caesarean section before the onset of labour. Production of both IL-6 and TNF-alpha was stimulated in a concentration-dependent manner by proinflammatory cytokine IL-1beta (0.01-10 ng/mL). Astragalus extract inhibited IL-6 production by approximately 75% from cells under IL-1beta-stimulated conditions. Treatment of amnion cells with IL-1beta (0.01-10 ng/mL) resulted in a significant increase in PGE(2) release. Incubation of the cells with the extract for 24 h significantly inhibited IL-1beta-induced PGE(2) production. A concentration-dependent increase in LTC(4) production by amnion cells occurred in response to IL-1beta. Astragalus extract blocked the effect of IL-1beta in LTC(4) production in human amnion. These results indicate that Astragali radix has a broad antiinflammatory effect in human amnion and may be considered a promising agent to protect preterm labour.

Amnion↗
PubMed2003

Antioxidant principles from the needles of red pine, Pinus densi fl ora.

Antioxidant activity of Pinus densiflora Sieb. et Zucc. (Pinaceae) was evaluated for potential to inhibit hydroxyl radicals, inhibit total reactive oxygen species generation in kidney homogenates using 2',7'-dichlorodihydro fluorescein diacetate (DCHF-DA) and scavenge authentic peroxynitrites. The methanolic extract of P. densiflora showed strong antioxidant activity in the tested model systems and thus fractionated with several solvents. The antioxidant activity potential of the individual fraction was in the order of ethyl acetate > n-butanol > water > dichloromethane fraction. The ethyl acetate soluble fraction exhibiting strong antioxidant activity was further puri fi ed by repeated silica gel and Sephadex LH-20 column chromatographies. An active lignan (+)-isolarisiresinol xylopyranoside, as well as two active flavonoids [kaempferol 3-O-beta-galactopyranoside and its 6"-acetyl derivative], were isolated.

Antioxidants↗